FastQCFastQC Report
Thu 26 May 2016
SRR936177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834328
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC3800.019.6142234
CGCGGAA3500.019.27107459
CGCGCTT4100.019.04743638
TAACGCG950.006640901318.68703752
ATTCGCG3850.018.4419456
TCGCGCT4000.017.74967437
CGATCGT4600.016.9755131
AATTCGC4400.016.9430955
CAATTCG4400.016.13817254
ACGCGAA1553.6398103E-416.03405654
TCGCGGA4500.015.77728458
ATTCGTC4500.015.7750234
CGTTACG2301.9169129E-615.43308911
GACGCAT2805.7771103E-815.21618948
CTCGCGC4700.015.10433136
CCTCGCG4750.014.94494935
GACGCGA3654.6020432E-1014.59753369
TCGAACT12450.014.54095324
CGAACTC12750.014.19825725
GATTCGT5050.014.05694833
Produced by FastQC (version 0.11.5)