##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936177_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3834328 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.39202592996739 39.0 36.0 41.0 29.0 41.0 2 36.250819178745274 39.0 36.0 41.0 29.0 41.0 3 36.158584242140996 39.0 36.0 41.0 29.0 41.0 4 36.043476979538525 39.0 36.0 41.0 28.0 41.0 5 35.97081000895072 39.0 35.0 41.0 28.0 41.0 6 35.8760713741756 39.0 35.0 40.0 27.0 41.0 7 35.71688102843575 39.0 35.0 40.0 27.0 41.0 8 35.54297910872518 39.0 35.0 40.0 27.0 41.0 9 35.46076757126673 38.0 35.0 40.0 27.0 41.0 10-11 35.68162895819033 39.0 35.0 40.0 27.0 41.0 12-13 35.870707461646475 39.0 35.0 41.0 28.0 41.0 14-15 35.8253082417571 39.0 35.0 41.0 27.0 41.0 16-17 35.667504710082184 39.0 35.0 41.0 27.0 41.0 18-19 35.53085455913005 39.0 35.0 41.0 26.5 41.0 20-21 35.35692225599897 39.0 35.0 41.0 26.0 41.0 22-23 35.13425351195829 39.0 35.0 41.0 25.0 41.0 24-25 34.93353385521531 39.0 34.0 41.0 24.0 41.0 26-27 34.0153168951639 38.0 33.0 40.0 23.5 40.5 28-29 34.052495769793296 38.0 33.5 40.0 23.0 40.5 30-31 34.425278432100754 38.0 34.0 40.0 23.0 41.0 32-33 34.27257109981201 38.0 34.0 40.0 23.0 41.0 34-35 34.00687199947422 38.0 33.0 40.0 22.0 41.0 36-37 33.71718577544748 38.0 33.0 40.0 20.5 41.0 38-39 33.43472480705876 37.0 33.0 40.0 20.0 41.0 40-41 33.06354920601471 37.0 32.0 40.0 18.5 41.0 42-43 32.71434290441506 36.0 32.0 39.0 17.5 41.0 44-45 32.353015052442046 36.0 32.0 39.0 14.0 41.0 46-47 31.970646225362046 35.0 31.0 39.0 11.5 40.0 48-49 31.50449479022139 35.0 31.0 38.0 8.0 40.0 50-51 31.062615665639456 35.0 31.0 37.0 6.5 39.5 52-53 30.607366140820503 35.0 30.0 37.0 2.0 39.0 54-55 30.14710752444757 34.5 30.0 36.0 2.0 39.0 56-57 29.69582088438965 34.0 29.5 36.0 2.0 37.5 58-59 29.264752389466942 34.0 29.0 35.0 2.0 37.0 60-61 28.907852953633597 34.0 29.0 35.0 2.0 36.5 62-63 28.56352025700462 34.0 29.0 35.0 2.0 36.0 64-65 28.198538700914476 34.0 28.5 35.0 2.0 36.0 66-67 27.81955964643609 34.0 27.0 35.0 2.0 35.5 68-69 27.465199247430057 34.0 27.0 35.0 2.0 35.0 70-71 27.04374912631366 33.0 26.0 35.0 2.0 35.0 72-73 26.562067720862693 33.0 25.0 35.0 2.0 35.0 74-75 25.98684084929615 33.0 24.5 35.0 2.0 35.0 76-77 24.294930558888026 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8596041275441095 1101 2 0.8205973215151303 1101 3 0.8297227220818968 1101 4 0.8241647380871058 1101 5 0.9610284642031033 1101 6 0.9120384455933817 1101 7 0.8625990979724634 1101 8 0.9487273502038107 1101 9 1.085569595552215 1101 10-11 0.9463584492786907 1101 12-13 0.944803017966187 1101 14-15 1.0846341684913838 1101 16-17 1.202821891606341 1101 18-19 1.1119394779534915 1101 20-21 0.9409811158638206 1101 22-23 1.026364913798595 1101 24-25 1.072222341081968 1101 26-27 1.0484273643323334 1101 28-29 0.9980205882203919 1101 30-31 1.1501393891724447 1101 32-33 1.190145543285439 1101 34-35 1.1159921539643278 1101 36-37 1.0966952683897944 1101 38-39 1.1589071535087214 1101 40-41 1.103431132920413 1101 42-43 1.0067982306121692 1101 44-45 1.0251672776446412 1101 46-47 0.9416291730783968 1101 48-49 1.1397984624993178 1101 50-51 0.9624039738138102 1101 52-53 1.0847876568228259 1101 54-55 1.0432807237577997 1101 56-57 1.0885941810512882 1101 58-59 0.9408232398534686 1101 60-61 0.8663118751467884 1101 62-63 0.785273052525234 1101 64-65 0.7827681380220746 1101 66-67 0.8791340809560211 1101 68-69 0.9116590648448728 1101 70-71 0.924857789366456 1101 72-73 0.9754149963032965 1101 74-75 0.9812361927754125 1101 76-77 1.1327462249766604 1102 1 0.7302288696305084 1102 2 0.7840315483307236 1102 3 0.6030865066075464 1102 4 0.8038872230527758 1102 5 0.8201595329970601 1102 6 0.8515577062333364 1102 7 0.6259442431691298 1102 8 0.8677726178080079 1102 9 0.8159174485519003 1102 10-11 0.7268532550726405 1102 12-13 0.7211320844232816 1102 14-15 0.8392884947167403 1102 16-17 0.7248590149365768 1102 18-19 0.8678996004537609 1102 20-21 0.8138407227687239 1102 22-23 0.7518648604771343 1102 24-25 0.8219810904386335 1102 26-27 0.7002970350838922 1102 28-29 0.7106710764595334 1102 30-31 0.7421520947498763 1102 32-33 0.6688723078781322 1102 34-35 0.8442471395211655 1102 36-37 0.7416469139365276 1102 38-39 0.737724825400889 1102 40-41 0.7630114060654023 1102 42-43 0.7825587279855029 1102 44-45 0.8329455751134986 1102 46-47 0.654123753994881 1102 48-49 0.7285975356331882 1102 50-51 0.7445094355544981 1102 52-53 0.5858000401531456 1102 54-55 0.5699459472674668 1102 56-57 0.5993367172055599 1102 58-59 0.6200242177227402 1102 60-61 0.47483481844520625 1102 62-63 0.5891420753050909 1102 64-65 0.6209570571264607 1102 66-67 0.5752792579137314 1102 68-69 0.6602135895188006 1102 70-71 0.6536000577542467 1102 72-73 0.6897756835474134 1102 74-75 0.7776251415543491 1102 76-77 0.9015942031658639 1103 1 0.3280954369452189 1103 2 0.26126452113688714 1103 3 0.37557627034559005 1103 4 0.39544804601813155 1103 5 0.5162443509647119 1103 6 0.46105720194690036 1103 7 0.4574607340882082 1103 8 0.3510607911621193 1103 9 0.34893946027843725 1103 10-11 0.37377837794541335 1103 12-13 0.4503513906907344 1103 14-15 0.4472388149762594 1103 16-17 0.48593440575856306 1103 18-19 0.5524410573146881 1103 20-21 0.5361380861786031 1103 22-23 0.5079712423348681 1103 24-25 0.5130921482358772 1103 26-27 0.24408989002251502 1103 28-29 0.2582302266239864 1103 30-31 0.5078291463300957 1103 32-33 0.3502228358735593 1103 34-35 0.5389017973966901 1103 36-37 0.5047490940345156 1103 38-39 0.3909216344720221 1103 40-41 0.29454826601678263 1103 42-43 0.3118677897116626 1103 44-45 0.39757369209289806 1103 46-47 0.18031790615390264 1103 48-49 0.2341814729812377 1103 50-51 0.2181679489632451 1103 52-53 0.16476739136983198 1103 54-55 0.24111623990413023 1103 56-57 0.31838463194351974 1103 58-59 0.227894379019137 1103 60-61 0.2764640117374455 1103 62-63 0.24786721849985938 1103 64-65 0.17450404420317867 1103 66-67 0.17746504794993712 1103 68-69 0.18586822105100254 1103 70-71 0.263020006046073 1103 72-73 0.29281071640129497 1103 74-75 0.3543506761356028 1103 76-77 0.4639718008055027 1104 1 0.4229199716816652 1104 2 0.41409483061713104 1104 3 0.3374598271901874 1104 4 0.3779773193307818 1104 5 0.2146061737206395 1104 6 0.1520472494907068 1104 7 0.4810386843989036 1104 8 0.4769967693203512 1104 9 0.36821818591273825 1104 10-11 0.38081565857746114 1104 12-13 0.4440252300446801 1104 14-15 0.34704611457836876 1104 16-17 0.37605636129151065 1104 18-19 0.4633412108392392 1104 20-21 0.2179724023406706 1104 22-23 0.32652743102186577 1104 24-25 0.2552782679868173 1104 26-27 0.034660196341825156 1104 28-29 0.08296334955690554 1104 30-31 0.19260187492564285 1104 32-33 0.31102509455145366 1104 34-35 0.33786397322774775 1104 36-37 0.15067388804791193 1104 38-39 0.23275333592609826 1104 40-41 0.271417189003337 1104 42-43 0.25032969668447436 1104 44-45 0.09289927472729431 1104 46-47 -0.001323530520693339 1104 48-49 0.09383308104904486 1104 50-51 0.06690368839506178 1104 52-53 0.06856668745820116 1104 54-55 0.13168385987960463 1104 56-57 0.08699810691284071 1104 58-59 -0.07107921377322413 1104 60-61 -0.15406552483690206 1104 62-63 -0.07201651653888419 1104 64-65 -0.12834858431710572 1104 66-67 -0.009241400484128803 1104 68-69 -0.07164134121374488 1104 70-71 -0.18573919412191486 1104 72-73 -0.25955937760730663 1104 74-75 -0.3176586956225975 1104 76-77 -0.2512841249332105 1105 1 0.21335053245847746 1105 2 0.2530497962367946 1105 3 0.246692528373174 1105 4 0.42449142740156276 1105 5 0.32038671010194975 1105 6 0.16500219911331016 1105 7 0.13405186330732022 1105 8 0.31284563979485114 1105 9 0.3709216884682718 1105 10-11 0.3339562807029921 1105 12-13 0.28935502269617075 1105 14-15 0.19778291234091228 1105 16-17 0.19759586468955348 1105 18-19 0.34169426054475593 1105 20-21 0.4556220081465412 1105 22-23 0.4339849921639498 1105 24-25 0.42165965381182247 1105 26-27 0.5171714142777191 1105 28-29 0.5346629035056409 1105 30-31 0.4863638227272489 1105 32-33 0.6015636354599252 1105 34-35 0.6447528390097759 1105 36-37 0.5624981123906352 1105 38-39 0.5477927020160678 1105 40-41 0.6531109573499592 1105 42-43 0.28939450875905237 1105 44-45 0.17140083044104415 1105 46-47 0.16327684967602352 1105 48-49 0.22094969159270406 1105 50-51 0.30544779627920704 1105 52-53 0.2392686116374847 1105 54-55 0.2708609179675854 1105 56-57 0.33211300577145053 1105 58-59 0.294364211303229 1105 60-61 0.11210549969458228 1105 62-63 0.05904070226618785 1105 64-65 0.3583125367032949 1105 66-67 0.42173396671497443 1105 68-69 0.028485568768154224 1105 70-71 -0.1934217805260161 1105 72-73 -0.12075291799587617 1105 74-75 -0.26210225134167686 1105 76-77 0.086969636374711 1106 1 -0.034326848283420475 1106 2 -0.0752994192091947 1106 3 -0.3004617486198171 1106 4 -0.04556525233253694 1106 5 -0.26349126893732233 1106 6 0.012251179426804981 1106 7 0.08690647745225988 1106 8 0.11594173724578383 1106 9 -0.08917031194631164 1106 10-11 -0.19614212641154438 1106 12-13 -0.09944923102744241 1106 14-15 -0.047286062566776366 1106 16-17 0.0494510895286453 1106 18-19 -0.0019621919826633416 1106 20-21 -0.16763520180422375 1106 22-23 -0.08093573445032831 1106 24-25 0.08317403306813986 1106 26-27 0.08869160071726157 1106 28-29 0.12003052919585144 1106 30-31 0.016306888960635035 1106 32-33 -0.007131414767378885 1106 34-35 -0.15668569629972495 1106 36-37 -0.0359326271691387 1106 38-39 -0.12588653214703527 1106 40-41 -0.06306689995692949 1106 42-43 -0.11923583938742155 1106 44-45 -0.2448983781968721 1106 46-47 -0.035108189434822634 1106 48-49 -0.08383940553509817 1106 50-51 -0.1147617348174208 1106 52-53 -0.16278331057572615 1106 54-55 -0.06660577837007864 1106 56-57 -0.05852345744565923 1106 58-59 -0.14805627143983813 1106 60-61 -0.20003275800818798 1106 62-63 -0.2267424444040529 1106 64-65 -0.1447391558645279 1106 66-67 -0.15583244754498793 1106 68-69 -0.36440327371746406 1106 70-71 -0.46896347945481054 1106 72-73 -0.4838687690149506 1106 74-75 -0.5573031453328952 1106 76-77 -0.4573388414015085 1107 1 0.19920992929250758 1107 2 0.22615061355755728 1107 3 0.2058645170480915 1107 4 0.19009410610262023 1107 5 0.2487340502274975 1107 6 -0.0380820875434722 1107 7 0.0716139408074099 1107 8 0.22531116378603144 1107 9 0.1785859531409173 1107 10-11 0.2770052037197672 1107 12-13 0.31001548871308415 1107 14-15 0.3219963454167001 1107 16-17 0.28565949855734374 1107 18-19 0.2932694150816886 1107 20-21 0.20596339710576927 1107 22-23 0.22390140646331957 1107 24-25 0.08127646444459202 1107 26-27 0.06177878932935954 1107 28-29 0.2954343069127958 1107 30-31 0.4543476441722092 1107 32-33 0.4940501587006665 1107 34-35 0.5431272428370519 1107 36-37 0.5553694574603512 1107 38-39 0.4597290681482775 1107 40-41 0.43870727485994365 1107 42-43 0.5333919302332291 1107 44-45 0.4359974608395305 1107 46-47 0.2970871211857542 1107 48-49 0.4436333441351543 1107 50-51 0.3674586693335904 1107 52-53 0.5408942396320704 1107 54-55 0.4936570608073474 1107 56-57 0.5197165113637041 1107 58-59 0.5624411196625374 1107 60-61 0.5778962376699148 1107 62-63 0.5389581213409969 1107 64-65 0.5367461737524728 1107 66-67 0.6268342481555145 1107 68-69 0.48050782052636976 1107 70-71 0.47056007528226473 1107 72-73 0.48553060385417623 1107 74-75 0.40197741692809785 1107 76-77 0.2861404699158818 1108 1 0.2519853060130899 1108 2 0.3069277258350027 1108 3 0.197938910803515 1108 4 0.3493924612975974 1108 5 0.21537215042798863 1108 6 0.25773462654274226 1108 7 0.16390691899668042 1108 8 0.34247211009294176 1108 9 0.2552975515447571 1108 10-11 0.10266166244216635 1108 12-13 0.21608622964428292 1108 14-15 0.24009661681536443 1108 16-17 0.2768580255499913 1108 18-19 0.23501051780440463 1108 20-21 0.09215637601892723 1108 22-23 0.1680529432687834 1108 24-25 0.2068998754933915 1108 26-27 -0.008513036253965822 1108 28-29 0.1924806101456582 1108 30-31 0.23701981967086994 1108 32-33 0.2077270425694877 1108 34-35 0.31493689769199307 1108 36-37 0.35707414569462514 1108 38-39 0.24983113478758412 1108 40-41 0.25742940981352547 1108 42-43 0.22784653946775535 1108 44-45 0.11786315196211206 1108 46-47 0.16844040938495652 1108 48-49 0.22174683903522308 1108 50-51 0.2765506427672193 1108 52-53 0.16703386361543338 1108 54-55 0.31092240939825544 1108 56-57 0.18286705526163516 1108 58-59 0.17489070423244968 1108 60-61 0.2794468265375052 1108 62-63 0.3402530376278534 1108 64-65 0.24761976691803156 1108 66-67 0.10300465212454668 1108 68-69 0.14847251077866375 1108 70-71 0.05439450363063969 1108 72-73 0.06733859081356641 1108 74-75 0.04428705929967691 1108 76-77 0.001287990032931674 1109 1 -0.08928097039726168 1109 2 -0.019454008760448005 1109 3 -0.1690269081441116 1109 4 0.05949405556572174 1109 5 0.06591619014644579 1109 6 0.04119134026534965 1109 7 -0.15322232449666018 1109 8 0.03909001595920358 1109 9 0.09757256841556483 1109 10-11 -0.03380538625243901 1109 12-13 0.017558589797708635 1109 14-15 0.08113436948193709 1109 16-17 0.12046019119906504 1109 18-19 -0.01406783419576385 1109 20-21 0.049049330829504356 1109 22-23 0.04579485233265501 1109 24-25 0.0052040460373987685 1109 26-27 0.03696777068685719 1109 28-29 0.10616743553705277 1109 30-31 0.1666713052112314 1109 32-33 0.07960329409852562 1109 34-35 0.2112058752930892 1109 36-37 0.26146305444650153 1109 38-39 0.22508756892656834 1109 40-41 0.3381895004040061 1109 42-43 0.37850003003191546 1109 44-45 0.2840995706571334 1109 46-47 0.1050752927326073 1109 48-49 0.3025913731582115 1109 50-51 0.2664509600228975 1109 52-53 0.26359692235133636 1109 54-55 0.25235940564551385 1109 56-57 0.37616843154445334 1109 58-59 0.32592188182990967 1109 60-61 0.19302257593000505 1109 62-63 0.24843503244827048 1109 64-65 0.2993012492066569 1109 66-67 0.2977606836980513 1109 68-69 0.20941768841936437 1109 70-71 0.20695522243232034 1109 72-73 0.08661305083672843 1109 74-75 -0.05080348177897065 1109 76-77 0.0041706681479318775 1110 1 -0.36386875488011583 1110 2 -0.38221295215012674 1110 3 -0.5716576379663891 1110 4 -0.32469125063661863 1110 5 -0.3128654107772135 1110 6 -0.5093314363338877 1110 7 -0.505547745138955 1110 8 -0.5151358989688362 1110 9 -0.5195764101606244 1110 10-11 -0.45082120557692207 1110 12-13 -0.4532185274337266 1110 14-15 -0.5600546866367679 1110 16-17 -0.6273416446271014 1110 18-19 -0.4688389859142532 1110 20-21 -0.6082516312905071 1110 22-23 -0.7836519996665956 1110 24-25 -0.645564884026939 1110 26-27 -0.5323690674324482 1110 28-29 -0.48296287516741643 1110 30-31 -0.42253933584449044 1110 32-33 -0.32635655162950883 1110 34-35 -0.17850203318455726 1110 36-37 -0.20166662680724556 1110 38-39 -0.19018201596306739 1110 40-41 -0.2172558743513875 1110 42-43 -0.1713671809023296 1110 44-45 -0.18422291084332443 1110 46-47 -0.17587460845467007 1110 48-49 -0.12333051437552456 1110 50-51 -0.21822280317574894 1110 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2314 6 -0.6020823566855569 2314 7 -0.5941283268898943 2314 8 -0.5506609631587196 2314 9 -0.5287677477480983 2314 10-11 -0.4607331894997344 2314 12-13 -0.6385596982513633 2314 14-15 -0.7597481491055973 2314 16-17 -1.245895669785078 2314 18-19 -0.8514125125552638 2314 20-21 -0.6140934675843965 2314 22-23 -0.5411331164008928 2314 24-25 -0.6488793054685758 2314 26-27 -0.5739078279623868 2314 28-29 -0.7076264260770202 2314 30-31 -0.6943952286454618 2314 32-33 -0.8400233808918074 2314 34-35 -0.7277430072011128 2314 36-37 -0.8687153723954637 2314 38-39 -0.8434510938334654 2314 40-41 -0.6841705774311535 2314 42-43 -0.6441203892514551 2314 44-45 -0.7255393775340195 2314 46-47 -0.5711363197643493 2314 48-49 -0.5529105221493005 2314 50-51 -0.4360489057917043 2314 52-53 -0.39643174052083197 2314 54-55 -0.4308075559387028 2314 56-57 -0.40363901757968534 2314 58-59 -0.4637808286498277 2314 60-61 -0.4789004929951268 2314 62-63 -0.3729335105530609 2314 64-65 -0.39968010125479836 2314 66-67 -0.39557399275945215 2314 68-69 -0.3180281521217836 2314 70-71 -0.16891519987226644 2314 72-73 -0.20632967905279642 2314 74-75 -0.2627285245631583 2314 76-77 -0.2518445938217049 2315 1 -1.4472375446955539 2315 2 -1.300435474316437 2315 3 -1.2312810493958892 2315 4 -1.0897732057623912 2315 5 -1.0838790943683776 2315 6 -1.0660438444734055 2315 7 -1.200155164076243 2315 8 -1.1544469758662714 2315 9 -1.1245348033626712 2315 10-11 -1.1951849303895017 2315 12-13 -1.2903857560778036 2315 14-15 -1.5435437673979635 2315 16-17 -1.4618004471191384 2315 18-19 -1.2501058590744591 2315 20-21 -1.2946053138247677 2315 22-23 -1.291092952352507 2315 24-25 -1.4305471160996959 2315 26-27 -1.3712020718925473 2315 28-29 -1.3020567495196715 2315 30-31 -1.4570157778058288 2315 32-33 -1.4343066050909883 2315 34-35 -1.3799600204386095 2315 36-37 -1.579344426704857 2315 38-39 -1.5110077749772124 2315 40-41 -1.495165660946185 2315 42-43 -1.6384398582312087 2315 44-45 -1.5100181647503774 2315 46-47 -1.4529458153055899 2315 48-49 -1.3048663972753474 2315 50-51 -1.1957310176959233 2315 52-53 -1.2595788061464397 2315 54-55 -1.3407616177276473 2315 56-57 -1.3812607585691303 2315 58-59 -1.2176202329346317 2315 60-61 -1.2847131472635063 2315 62-63 -1.3886607974247127 2315 64-65 -1.336753553570567 2315 66-67 -1.2532547778563696 2315 68-69 -1.4011833164413119 2315 70-71 -1.6287298658062141 2315 72-73 -2.941582884870275 2315 74-75 -3.4341790911929877 2315 76-77 -3.200802930146576 2316 1 -3.5210493598032713 2316 2 -3.3221082659955243 2316 3 -2.785945800333316 2316 4 -2.7854831817348185 2316 5 -3.4818794445986327 2316 6 -3.0199286331797097 2316 7 -3.3020620185867173 2316 8 -3.2494102698254252 2316 9 -2.797940544190986 2316 10-11 -2.8923507768394074 2316 12-13 -2.980736829003284 2316 14-15 -3.114553601377885 2316 16-17 -4.1798874760939775 2316 18-19 -4.079345918741531 2316 20-21 -3.4748680078407865 2316 22-23 -3.6216472793416727 2316 24-25 -3.548088140920896 2316 26-27 -3.358726903431144 2316 28-29 -3.41976906549003 2316 30-31 -3.68619749511884 2316 32-33 -3.7494480891235753 2316 34-35 -3.691568980845254 2316 36-37 -3.592864839668792 2316 38-39 -3.600619025878629 2316 40-41 -3.4988591625618497 2316 42-43 -3.714662264272306 2316 44-45 -3.559531040625643 2316 46-47 -3.227514658031904 2316 48-49 -3.373006090572648 2316 50-51 -3.820822294950826 2316 52-53 -4.953350742468313 2316 54-55 -4.061916939100065 2316 56-57 -3.7775134106733965 2316 58-59 -3.8708363998155484 2316 60-61 -3.709193496129185 2316 62-63 -3.7535075383133716 2316 64-65 -3.5213464767130134 2316 66-67 -3.7262573830763195 2316 68-69 -3.76362813408565 2316 70-71 -3.494765053471877 2316 72-73 -3.3998166749707366 2316 74-75 -3.4812979235412485 2316 76-77 -3.4663042576935403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 109195.0 3 8678.0 4 10724.0 5 12368.0 6 14253.0 7 15421.0 8 17299.0 9 17350.0 10 19431.0 11 21425.0 12 18584.0 13 17285.0 14 17504.0 15 18307.0 16 18967.0 17 19707.0 18 21149.0 19 22253.0 20 23848.0 21 25829.0 22 27885.0 23 32426.0 24 37535.0 25 43762.0 26 51202.0 27 60458.0 28 71360.0 29 84233.0 30 100193.0 31 120920.0 32 146949.0 33 184709.0 34 244275.0 35 326785.0 36 463362.0 37 631167.0 38 588620.0 39 167466.0 40 1444.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.885428618601317 26.594074544338675 27.99146463957508 22.529032197484934 2 22.66876067971434 26.728141130427957 28.049393458739363 22.553704731118344 3 22.750062992758572 27.01846796394362 27.576560329325623 22.654908713972187 4 22.553378740737497 26.913825052116263 27.811096017044473 22.72170019010177 5 22.700450489810834 26.990967511053576 27.86847189505421 22.440110104081377 6 23.017536117118894 26.78775434829558 27.715369872475037 22.47933966211049 7 22.928899738868388 26.566639686631632 27.851143243118457 22.65331733138152 8 22.715472942305787 26.8577286457328 27.76886839208485 22.657930019876567 9 22.618927442984656 26.635610190885423 27.809057712718992 22.93640465341093 10-11 22.98803890749434 26.853863692299146 27.644044189276556 22.51405321092996 12-13 22.955548877922965 26.74646176768412 27.611658624814606 22.686330729578312 14-15 22.76518654369483 26.907315594740684 27.692332370325516 22.63516549123897 16-17 23.102263748247125 26.674402588482216 27.68412817582608 22.53920548744458 18-19 22.847248479217306 26.650154050940433 27.615739059109572 22.88685841073269 20-21 22.93737478609291 26.778180954964732 27.555707145540296 22.72873711340206 22-23 23.032744089881614 26.890531615561265 27.2615080898644 22.815216204692724 24-25 23.0100959880551 26.91193556347268 27.32482401668196 22.753144431790258 26-27 23.032862835695212 27.002719265568736 27.207172871621655 22.757245027114394 28-29 23.11672513082382 26.976911417999034 27.151886567797945 22.754476883379198 30-31 22.97082433594515 26.86887239719112 27.270321751557063 22.88998151530667 32-33 22.935169375919756 27.118705296802364 27.18657356038163 22.759551766896248 34-35 23.04603528696005 26.966152192866055 27.26436397853073 22.723448541643165 36-37 23.046422616973373 27.012630077644907 27.34910523690981 22.591842068471912 38-39 22.959770564667583 26.959303660114355 27.395715955093692 22.68520982012437 40-41 22.827502830045958 26.881354561721082 27.371852388976126 22.919290219256833 42-43 22.795147203206888 27.043000739746066 27.189567493742416 22.97228456330463 44-45 22.87940794075234 26.904129591342073 27.369711374246812 22.846751093658767 46-47 22.877422845375627 26.72479773861678 27.377167980641758 23.020611435365833 48-49 22.852301985428195 26.84404045463201 27.224522164223686 23.079135395716108 50-51 22.825037846101868 26.948459512008675 27.143139468673994 23.08336317321546 52-53 22.862883100490265 26.87630500524546 27.22248442994866 23.038327464315618 54-55 22.7678595303286 26.889670383221357 27.243690707204543 23.098779379245496 56-57 22.849291791427202 27.027355213974243 27.157444853744366 22.965908140854186 58-59 22.982179941468857 27.0061537390065 27.12755128191919 22.884115037605458 60-61 22.988463620297246 26.849544479297776 27.09701906209037 23.064972838314606 62-63 22.804082961503624 26.895953247544675 27.281117868278372 23.018845922673332 64-65 22.936719903123596 26.838880370058682 27.159932153126075 23.06446757369165 66-67 22.923009777401653 26.92816000722107 27.219507219374172 22.92932299600311 68-69 22.94481317586356 26.739074480876646 27.306476014324492 23.009636328935297 70-71 22.818989491235957 26.75572163028382 27.338234248433206 23.087054630047017 72-73 22.856065588190805 26.605468666901672 27.339301741411415 23.199164003496108 74-75 22.736591772329078 26.57469763646736 27.26841330648819 23.420297284715378 76-77 22.76374675508582 26.511950550705492 27.426138640222707 23.298164053985975 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3037.0 1 1957.5 2 863.5 3 910.5 4 972.0 5 1032.5 6 1116.0 7 1274.5 8 1467.5 9 1525.0 10 1597.5 11 1749.5 12 1969.5 13 2110.0 14 2267.5 15 2577.0 16 3064.5 17 3400.0 18 3688.5 19 4633.5 20 6027.0 21 8011.0 22 9258.0 23 11203.0 24 15549.5 25 21742.5 26 25534.0 27 29747.5 28 40470.0 29 52139.5 30 57300.0 31 65249.5 32 80317.5 33 94934.5 34 109706.0 35 116979.0 36 124841.5 37 138741.0 38 147657.0 39 150536.0 40 154767.0 41 163013.0 42 171364.0 43 175700.0 44 177713.0 45 181530.5 46 184487.0 47 185967.0 48 186295.0 49 186060.5 50 182516.5 51 175789.5 52 172372.0 53 169669.5 54 159572.5 55 144134.0 56 127639.0 57 119188.0 58 111146.0 59 92823.5 60 74169.5 61 65796.0 62 58145.0 63 43291.5 64 30833.5 65 25578.0 66 22147.5 67 16533.5 68 12201.5 69 8749.0 70 7445.0 71 6424.5 72 4456.0 73 3052.0 74 2596.0 75 2332.0 76 1694.0 77 1117.0 78 914.0 79 722.0 80 440.0 81 309.0 82 240.0 83 212.0 84 185.0 85 136.0 86 111.5 87 109.0 88 104.0 89 91.0 90 71.5 91 60.0 92 62.0 93 58.0 94 53.0 95 48.5 96 43.0 97 51.0 98 63.0 99 126.0 100 185.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010301674765434778 2 0.014109382400253709 3 0.01444842486088827 4 0.015439472053512376 5 0.01306617482907044 6 0.010745037983187667 7 0.016404439056856897 8 0.017421566438760586 9 0.017395486249481003 10-11 0.020173026407756457 12-13 0.016534840003254808 14-15 0.008697743124740502 16-17 0.0069503704430085265 18-19 0.018086611265389917 20-21 0.023159208080268563 22-23 0.020772870761186838 24-25 0.018934217416976325 26-27 0.02451537792280681 28-29 0.009258467194251508 30-31 0.008997665301455692 32-33 0.011214481390220138 34-35 0.012401130002441106 36-37 0.012101207825725917 38-39 0.014813547510802414 40-41 0.012205528582844242 42-43 0.015126509782157396 44-45 0.013626898898581445 46-47 0.02404593451577434 48-49 0.02342000997306438 50-51 0.03574289940766674 52-53 0.03883340183729718 54-55 0.02799708319163097 56-57 0.021320554736058053 58-59 0.016756521612131252 60-61 0.020942391991504116 62-63 0.02691475533652833 64-65 0.025819387386785898 66-67 0.02871428839681947 68-69 0.04153270142773388 70-71 0.05347742811778231 72-73 0.08375652787137668 74-75 0.08396516938561333 76-77 0.08692527086884586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3834328.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.69258401842193 #Duplication Level Percentage of deduplicated Percentage of total 1 75.85085219850146 45.277333677279536 2 13.611763160862933 16.250426322373425 3 4.706429012612727 8.428167277863716 4 2.0839607138090956 4.9758800000056 5 1.144304146499796 3.415323570378384 6 0.6556523343744383 2.3482549233912375 7 0.42201121253164386 1.7633657832532887 8 0.2821837757067495 1.347542299200854 9 0.21516052468902275 1.1559138927702515 >10 0.949495728531238 9.967277291801386 >50 0.05453205194855494 2.229950992554674 >100 0.02251673190623865 2.2839119514936628 >500 9.632683473566319E-4 0.3736714314093232 >1k 1.75139678818545E-4 0.18298058622459956 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.216037855916343E-5 0.0 2 0.0 0.0 0.0 1.564811356774903E-4 0.0 3 0.0 0.0 0.0 2.3472170351623544E-4 2.6080189279581715E-5 4 2.6080189279581715E-5 0.0 0.0 4.955235963120526E-4 2.6080189279581715E-5 5 5.216037855916343E-5 0.0 0.0 0.0010692877604628504 2.6080189279581715E-5 6 5.216037855916343E-5 0.0 0.0 0.002399377413721518 2.6080189279581715E-5 7 5.216037855916343E-5 0.0 0.0 0.005294278423755089 2.6080189279581715E-5 8 5.216037855916343E-5 0.0 0.0 0.007928377540992841 7.824056783874515E-5 9 1.0432075711832686E-4 0.0 0.0 0.011005839875983484 7.824056783874515E-5 10 1.3040094639790858E-4 2.6080189279581715E-5 0.0 0.014839627700081997 1.3040094639790858E-4 11 1.82561324957072E-4 2.6080189279581715E-5 0.0 0.01898637779553549 1.3040094639790858E-4 12 1.82561324957072E-4 2.6080189279581715E-5 0.0 0.0246718590584843 1.3040094639790858E-4 13 1.82561324957072E-4 2.6080189279581715E-5 0.0 0.03270455735659547 1.3040094639790858E-4 14 2.3472170351623544E-4 2.6080189279581715E-5 0.0 0.04157182171165325 1.3040094639790858E-4 15 2.3472170351623544E-4 2.6080189279581715E-5 0.0 0.05231685969484092 1.564811356774903E-4 16 2.3472170351623544E-4 2.6080189279581715E-5 0.0 0.06267069483883486 1.564811356774903E-4 17 2.3472170351623544E-4 2.6080189279581715E-5 0.0 0.07378085547193668 1.564811356774903E-4 18 2.8688208207539885E-4 2.6080189279581715E-5 0.0 0.0840042896695327 1.564811356774903E-4 19 3.65122649914144E-4 2.6080189279581715E-5 0.0 0.09547957295254866 1.564811356774903E-4 20 3.9120283919372574E-4 2.6080189279581715E-5 0.0 0.10836318645666203 1.564811356774903E-4 21 3.9120283919372574E-4 2.6080189279581715E-5 0.0 0.1225247292354749 2.0864151423665372E-4 22 4.433632177528892E-4 2.6080189279581715E-5 0.0 0.13898132867089097 2.0864151423665372E-4 23 4.694434070324709E-4 2.6080189279581715E-5 0.0 0.15494240450999497 2.0864151423665372E-4 24 4.955235963120526E-4 2.6080189279581715E-5 0.0 0.1713207633775723 2.0864151423665372E-4 25 4.955235963120526E-4 2.6080189279581715E-5 0.0 0.19020282041598946 2.0864151423665372E-4 26 4.955235963120526E-4 2.6080189279581715E-5 0.0 0.20851111329025582 2.0864151423665372E-4 27 4.955235963120526E-4 2.6080189279581715E-5 0.0 0.22762789203218922 2.0864151423665372E-4 28 4.955235963120526E-4 2.6080189279581715E-5 0.0 0.24666643020628387 2.0864151423665372E-4 29 4.955235963120526E-4 2.6080189279581715E-5 0.0 0.26591360989461515 2.0864151423665372E-4 30 4.955235963120526E-4 2.6080189279581715E-5 0.0 0.2865169594254847 2.0864151423665372E-4 31 4.955235963120526E-4 2.6080189279581715E-5 0.0 0.3092067240987208 2.0864151423665372E-4 32 5.216037855916343E-4 2.6080189279581715E-5 0.0 0.334634908646313 2.0864151423665372E-4 33 5.216037855916343E-4 2.6080189279581715E-5 0.0 0.36034997527598056 2.0864151423665372E-4 34 5.216037855916343E-4 2.6080189279581715E-5 0.0 0.3877863343981005 2.0864151423665372E-4 35 5.216037855916343E-4 2.6080189279581715E-5 0.0 0.4165267029841996 2.0864151423665372E-4 36 5.216037855916343E-4 2.6080189279581715E-5 0.0 0.4481619725803322 2.0864151423665372E-4 37 5.216037855916343E-4 2.6080189279581715E-5 0.0 0.4810751714511643 2.0864151423665372E-4 38 5.216037855916343E-4 2.6080189279581715E-5 0.0 0.5134667665364048 2.3472170351623544E-4 39 5.216037855916343E-4 2.6080189279581715E-5 0.0 0.5460409229466023 2.3472170351623544E-4 40 5.216037855916343E-4 2.6080189279581715E-5 0.0 0.5814056596097151 2.6080189279581716E-4 41 5.216037855916343E-4 2.6080189279581715E-5 0.0 0.6195609765257433 2.8688208207539885E-4 42 5.476839748712161E-4 2.6080189279581715E-5 0.0 0.6603243123697294 2.8688208207539885E-4 43 5.476839748712161E-4 2.6080189279581715E-5 0.0 0.7069556908016216 2.8688208207539885E-4 44 5.476839748712161E-4 2.6080189279581715E-5 0.0 0.7573165363004939 2.8688208207539885E-4 45 5.476839748712161E-4 2.6080189279581715E-5 0.0 0.8074948204744091 3.129622713549806E-4 46 5.737641641507977E-4 2.6080189279581715E-5 0.0 0.8636976283719077 3.129622713549806E-4 47 5.737641641507977E-4 2.6080189279581715E-5 0.0 0.9212826863012241 3.129622713549806E-4 48 5.737641641507977E-4 5.216037855916343E-5 0.0 0.981710484862015 3.129622713549806E-4 49 6.520047319895428E-4 5.216037855916343E-5 0.0 1.0443290193222907 3.129622713549806E-4 50 6.520047319895428E-4 5.216037855916343E-5 0.0 1.10796468116447 3.129622713549806E-4 51 6.520047319895428E-4 5.216037855916343E-5 0.0 1.1762165365091353 3.390424606345623E-4 52 6.520047319895428E-4 5.216037855916343E-5 0.0 1.2453029579107473 3.390424606345623E-4 53 6.520047319895428E-4 5.216037855916343E-5 0.0 1.3218483134463197 3.390424606345623E-4 54 6.520047319895428E-4 5.216037855916343E-5 0.0 1.4057743625480137 3.65122649914144E-4 55 6.520047319895428E-4 5.216037855916343E-5 0.0 1.493038675877494 3.65122649914144E-4 56 7.041651105487063E-4 5.216037855916343E-5 0.0 1.588127045990849 3.65122649914144E-4 57 7.30245299828288E-4 5.216037855916343E-5 0.0 1.6852235906787316 3.9120283919372574E-4 58 7.30245299828288E-4 5.216037855916343E-5 0.0 1.789700828932736 4.433632177528892E-4 59 7.30245299828288E-4 5.216037855916343E-5 0.0 1.8906572416339968 4.433632177528892E-4 60 7.30245299828288E-4 5.216037855916343E-5 0.0 1.9975599374910022 4.433632177528892E-4 61 8.867264355057784E-4 5.216037855916343E-5 0.0 2.109756911771763 4.433632177528892E-4 62 8.867264355057784E-4 5.216037855916343E-5 0.0 2.227535046558354 4.433632177528892E-4 63 8.867264355057784E-4 5.216037855916343E-5 0.0 2.349929374847431 4.433632177528892E-4 64 8.867264355057784E-4 5.216037855916343E-5 0.0 2.4828340194161793 4.433632177528892E-4 65 8.867264355057784E-4 5.216037855916343E-5 0.0 2.618972607455596 4.433632177528892E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 380 0.0 19.614223 4 CGCGGAA 350 0.0 19.271074 59 CGCGCTT 410 0.0 19.047436 38 TAACGCG 95 0.0066409013 18.687037 52 ATTCGCG 385 0.0 18.44194 56 TCGCGCT 400 0.0 17.749674 37 CGATCGT 460 0.0 16.975513 1 AATTCGC 440 0.0 16.94309 55 CAATTCG 440 0.0 16.138172 54 ACGCGAA 155 3.6398103E-4 16.034056 54 TCGCGGA 450 0.0 15.777284 58 ATTCGTC 450 0.0 15.77502 34 CGTTACG 230 1.9169129E-6 15.433089 11 GACGCAT 280 5.7771103E-8 15.216189 48 CTCGCGC 470 0.0 15.104331 36 CCTCGCG 475 0.0 14.944949 35 GACGCGA 365 4.6020432E-10 14.597533 69 TCGAACT 1245 0.0 14.540953 24 CGAACTC 1275 0.0 14.198257 25 GATTCGT 505 0.0 14.056948 33 >>END_MODULE