FastQCFastQC Report
Thu 26 May 2016
SRR936177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834328
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53880.14052005983838628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39590.103251469357864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12650.035.527794100-101
GGTATCA74850.034.421251
CGGTATC10300.028.896458100-101
CGTACAT16700.028.515787100-101
GTATCAA133900.028.3511491
TACGCAG10750.026.025627100-101
ACGTATC5750.025.36355100-101
GCGTATC4700.024.697247100-101
TCGTACA9800.023.992905100-101
TCGAGTA5100.023.9274100-101
GCGTACA8300.023.308647100-101
ATCGTAC4150.022.94930398-99
CCTGTCG9950.022.72951796-97
ATCAACG166000.022.5429523
TCAACGC166750.022.369624
CCGTACA8300.022.232864100-101
CGAGTAC13900.022.054857100-101
CGCATAA2850.021.930891100-101
CAACGCA170850.021.83285
TATCAAC172350.021.7477572
Produced by FastQC (version 0.11.5)