FastQCFastQC Report
Thu 26 May 2016
SRR936176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3836501
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG1505.569673E-721.30342752
TCGCGCT3900.020.93298737
ATTCGCG3950.020.66989956
CGATCGT4000.019.520761
CTCGCGC4600.019.29054536
CGCGGAA3950.017.97687159
CCTCGCG4950.017.925435
AACGCGA2055.751954E-717.31895653
AATTCGC4950.016.49523755
ACGCGAA1956.8550853E-616.38490154
CGCGCTT5050.016.16691238
GACGTAA4400.016.13201731
ACGATCG3201.1332304E-915.53314159
CAATTCG5350.015.26194854
ATCGTTC6100.015.1282563
TCGCGGA4950.015.06204858
GTCGGGT5900.015.048717566
GTACGAC1901.00614016E-414.94684919
AGCGCGA3908.0035534E-1114.563786523
TAATTCG4700.014.34794735