##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936176_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3836501 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3667383378761 39.0 36.0 41.0 29.0 41.0 2 36.23127010784045 39.0 36.0 41.0 29.0 41.0 3 36.08733661218908 39.0 36.0 41.0 28.0 41.0 4 35.984269259932425 39.0 35.0 41.0 27.0 41.0 5 35.916174399537496 39.0 35.0 41.0 27.0 41.0 6 35.82617181645463 39.0 35.0 40.0 27.0 41.0 7 35.69598678587599 39.0 35.0 40.0 27.0 41.0 8 35.565716000074026 39.0 35.0 40.0 27.0 41.0 9 35.74544174496501 39.0 35.0 40.0 27.0 41.0 10-11 35.97215509653197 39.0 35.0 41.0 28.5 41.0 12-13 35.89933913740671 39.0 35.0 41.0 27.5 41.0 14-15 35.75006627132379 39.0 35.0 41.0 27.0 41.0 16-17 35.611686012853895 39.0 35.0 41.0 27.0 41.0 18-19 35.46828920414722 39.0 35.0 41.0 26.0 41.0 20-21 35.29498584778161 39.0 35.0 41.0 25.5 41.0 22-23 35.06016510878011 39.0 34.5 41.0 24.5 41.0 24-25 34.86119917602002 39.0 34.0 41.0 24.0 41.0 26-27 33.92468527963371 38.0 33.0 40.0 23.0 40.5 28-29 33.937114704257866 38.0 33.0 39.5 23.0 40.5 30-31 34.35008748857358 38.0 34.0 40.0 23.0 41.0 32-33 34.230492315784616 38.0 34.0 40.0 22.5 41.0 34-35 33.98144220475897 38.0 33.0 40.0 22.0 41.0 36-37 33.67272783194896 38.0 33.0 40.0 20.5 41.0 38-39 33.36659328382815 37.0 33.0 40.0 19.5 41.0 40-41 33.01143007652024 37.0 32.0 40.0 18.0 41.0 42-43 32.64578296734446 36.0 32.0 39.0 16.0 41.0 44-45 32.29294753213931 36.0 32.0 39.0 13.0 41.0 46-47 31.886920269276615 35.0 31.0 39.0 9.5 40.0 48-49 31.46590330616361 35.0 31.0 38.0 7.5 40.0 50-51 31.009375730646234 35.0 31.0 37.0 4.5 39.0 52-53 30.521993999219603 35.0 30.0 37.0 2.0 39.0 54-55 30.05808704337624 34.5 30.0 36.0 2.0 39.0 56-57 29.616221395485105 34.0 29.0 36.0 2.0 37.5 58-59 29.187568438011617 34.0 29.0 35.0 2.0 37.0 60-61 28.823225251342304 34.0 29.0 35.0 2.0 36.5 62-63 28.451156405276578 34.0 29.0 35.0 2.0 36.0 64-65 28.07911753444089 34.0 28.0 35.0 2.0 36.0 66-67 27.735051808926936 34.0 27.0 35.0 2.0 35.0 68-69 27.369380328585866 33.5 26.5 35.0 2.0 35.0 70-71 26.92243348822273 33.0 25.5 35.0 2.0 35.0 72-73 26.448642135112173 33.0 25.0 35.0 2.0 35.0 74-75 25.884433628454676 33.0 24.0 35.0 2.0 35.0 76-77 24.13162227769522 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9145079517257884 1101 2 0.8729223468114498 1101 3 0.9442962673654662 1101 4 0.8548015768343689 1101 5 0.9155532926638514 1101 6 0.9716034273098941 1101 7 0.9508527623576768 1101 8 0.8472498345619215 1101 9 0.7452907557206672 1101 10-11 0.8601406338976503 1101 12-13 1.0363022897949605 1101 14-15 1.0739012103977714 1101 16-17 1.0444520437877287 1101 18-19 1.0051236021216496 1101 20-21 1.092392299298453 1101 22-23 1.0510755880711073 1101 24-25 1.2737860236205663 1101 26-27 1.0857167361842457 1101 28-29 1.1156528080580301 1101 30-31 1.1994003728231633 1101 32-33 1.216273443704658 1101 34-35 1.2824979300228065 1101 36-37 1.1851926015659444 1101 38-39 1.276052998380834 1101 40-41 1.1534396012768156 1101 42-43 1.1782790651329194 1101 44-45 1.1203356409566467 1101 46-47 1.1974752713095462 1101 48-49 1.0925631590142189 1101 50-51 0.9997638351870854 1101 52-53 1.126062337879624 1101 54-55 1.1462216797070504 1101 56-57 1.2552394511753242 1101 58-59 1.344789955173308 1101 60-61 1.1862870351800332 1101 62-63 1.174656225031864 1101 64-65 1.1371035360076966 1101 66-67 1.1354925050883296 1101 68-69 1.0104321713147542 1101 70-71 1.0621698181961676 1101 72-73 1.0241319185212667 1101 74-75 1.223796577109912 1101 76-77 1.4417136970560342 1102 1 0.8954990228872859 1102 2 0.8571443264112233 1102 3 0.9621683396935481 1102 4 0.8859960520220724 1102 5 0.8954935053407098 1102 6 0.9239906203343295 1102 7 0.9040571235792996 1102 8 0.9893030561227505 1102 9 0.9257493869312157 1102 10-11 1.0124336964742966 1102 12-13 1.0771707015983765 1102 14-15 1.0325058737342019 1102 16-17 0.9630164002233812 1102 18-19 0.9722183270775275 1102 20-21 1.0069915858887484 1102 22-23 0.862028558368138 1102 24-25 0.9330780168304642 1102 26-27 0.855370743692248 1102 28-29 1.1038563092993954 1102 30-31 1.0753759830352436 1102 32-33 1.0311818558071977 1102 34-35 0.9856705093653275 1102 36-37 1.0244807996455734 1102 38-39 1.0416748201929238 1102 40-41 1.1550178520021888 1102 42-43 1.0394371220262286 1102 44-45 0.945431453974507 1102 46-47 0.9777592891373139 1102 48-49 0.8092367740720974 1102 50-51 0.8646136377655296 1102 52-53 0.9868920131809915 1102 54-55 1.0384965331365024 1102 56-57 0.9859966450971314 1102 58-59 1.0404957559270969 1102 60-61 0.9854352808203082 1102 62-63 0.8695920630265377 1102 64-65 1.057166314566519 1102 66-67 1.007791606222007 1102 68-69 0.8150502652993801 1102 70-71 0.9439125307982437 1102 72-73 0.9655026709666004 1102 74-75 0.8099209826018203 1102 76-77 0.8931489293254842 1103 1 0.514132249767961 1103 2 0.43969369398183744 1103 3 0.6053326779328003 1103 4 0.5257194786721158 1103 5 0.34803399986061123 1103 6 0.5056708240911831 1103 7 0.5716645611356554 1103 8 0.41672563246870453 1103 9 0.54079513523115 1103 10-11 0.6077163408589499 1103 12-13 0.6388824998819587 1103 14-15 0.5452629497227122 1103 16-17 0.43608417667996946 1103 18-19 0.454150353042877 1103 20-21 0.6441992152754281 1103 22-23 0.5044185702587427 1103 24-25 0.49073569309702236 1103 26-27 0.6326550154015891 1103 28-29 0.6408169661206884 1103 30-31 0.7604550681410984 1103 32-33 0.7496272937299651 1103 34-35 0.800826093100703 1103 36-37 0.7712615347077758 1103 38-39 0.6821876609701576 1103 40-41 0.8187859887693065 1103 42-43 0.745930434907244 1103 44-45 0.7441346234856994 1103 46-47 0.6759985992665953 1103 48-49 0.7356659818559592 1103 50-51 0.6386635567585266 1103 52-53 0.7457485391840564 1103 54-55 0.7418371289600607 1103 56-57 0.7600240222305246 1103 58-59 0.6848795099819398 1103 60-61 0.7128231013906969 1103 62-63 0.6799893386048126 1103 64-65 0.5689948635173785 1103 66-67 0.6490368329158827 1103 68-69 0.659220006400286 1103 70-71 0.6820020767638795 1103 72-73 0.7390801987981632 1103 74-75 0.831865707447438 1103 76-77 0.9840971602834507 1104 1 0.5687870683772474 1104 2 0.6079769928828256 1104 3 0.6036576563923504 1104 4 0.5933488635933841 1104 5 0.5267267823873851 1104 6 0.4211767576009109 1104 7 0.5653654534167458 1104 8 0.3943672687382431 1104 9 0.46764639997820723 1104 10-11 0.5397125128329137 1104 12-13 0.5853582947974445 1104 14-15 0.7806688606167071 1104 16-17 0.7724679754859238 1104 18-19 0.6331580909555967 1104 20-21 0.4891528876968536 1104 22-23 0.5793564463494647 1104 24-25 0.5883047439435884 1104 26-27 0.3451366487851928 1104 28-29 0.48233856137665754 1104 30-31 0.683524093319491 1104 32-33 0.6424245276724463 1104 34-35 0.6822684569588162 1104 36-37 0.6346309052271124 1104 38-39 0.5908280526803082 1104 40-41 0.5889178121667413 1104 42-43 0.7376951146725332 1104 44-45 0.6919299010973674 1104 46-47 0.5331500289836875 1104 48-49 0.473061522809175 1104 50-51 0.2941193610255475 1104 52-53 0.14798160061978294 1104 54-55 0.07140880350986123 1104 56-57 0.2051543194124399 1104 58-59 0.12865546431757124 1104 60-61 0.1478831510343248 1104 62-63 0.1746172333847369 1104 64-65 0.14372670051201197 1104 66-67 0.10677262974606805 1104 68-69 0.0043025399735547865 1104 70-71 -0.031080330549745128 1104 72-73 -0.062355345651479155 1104 74-75 -0.09155486760282017 1104 76-77 -0.004550397702335118 1105 1 0.07786301831959008 1105 2 0.46072289276686007 1105 3 0.23880798335213882 1105 4 0.24417122443236394 1105 5 0.16768133626815285 1105 6 0.33135133559049024 1105 7 0.3323352892878688 1105 8 0.18487621505511243 1105 9 0.2682023999952037 1105 10-11 0.4067343163641013 1105 12-13 0.4947065232380581 1105 14-15 0.38052033021112663 1105 16-17 0.2746302976736388 1105 18-19 0.34917477226387916 1105 20-21 0.2489346267940178 1105 22-23 0.2541552044875033 1105 24-25 0.3924464961577314 1105 26-27 0.2330500973748073 1105 28-29 0.4480804058542347 1105 30-31 0.6308561204047862 1105 32-33 0.687934278270184 1105 34-35 0.6007976659224212 1105 36-37 0.6431058513877019 1105 38-39 0.5949912687314054 1105 40-41 0.6948736427616495 1105 42-43 0.6312393058193635 1105 44-45 0.4169034023314211 1105 46-47 0.4879014244600235 1105 48-49 0.3463077164518893 1105 50-51 0.26946880634965353 1105 52-53 0.29564111060349063 1105 54-55 0.05407969766421061 1105 56-57 0.15347003757803535 1105 58-59 0.20400301336971083 1105 60-61 0.3667904210467796 1105 62-63 0.18327416443857913 1105 64-65 0.16309094754655362 1105 66-67 0.22776491616944838 1105 68-69 0.09454984162759672 1105 70-71 -0.11120869181440085 1105 72-73 -0.504316999762974 1105 74-75 -0.6088787014854731 1105 76-77 -0.5789487073393538 1106 1 0.18551871717627932 1106 2 0.2757991097825254 1106 3 0.201122829092391 1106 4 0.21946175495212827 1106 5 0.28280841549505453 1106 6 0.22371089697038826 1106 7 -0.12227266500818956 1106 8 0.1303187453974175 1106 9 0.10160513856265396 1106 10-11 -0.0644490080044946 1106 12-13 0.20901412776005657 1106 14-15 0.1253195786523449 1106 16-17 0.17755879213802928 1106 18-19 0.21997901347693016 1106 20-21 0.294089706195237 1106 22-23 0.26082137139452755 1106 24-25 0.13286707929070474 1106 26-27 0.00781132580183197 1106 28-29 0.17780344514587654 1106 30-31 0.3680471969735777 1106 32-33 0.24926218000810252 1106 34-35 0.26197290236562765 1106 36-37 0.28509145831012717 1106 38-39 0.47673871213047647 1106 40-41 0.394052492577174 1106 42-43 0.4550752526124242 1106 44-45 0.3572529467032268 1106 46-47 0.30686078173032527 1106 48-49 0.22170261440416894 1106 50-51 0.17428563710057077 1106 52-53 0.19801937127948221 1106 54-55 0.1755944838108725 1106 56-57 0.3154551284842064 1106 58-59 0.16803620894663496 1106 60-61 -0.09602112966227949 1106 62-63 -0.14444779373609862 1106 64-65 -0.048338332505377224 1106 66-67 -0.13523804038339904 1106 68-69 -0.10732310856472083 1106 70-71 -0.27604205868354725 1106 72-73 -0.29634392731570713 1106 74-75 -0.20037203992023578 1106 76-77 -0.15122708740702606 1107 1 -0.07152775156550462 1107 2 -0.012550259045994494 1107 3 0.1900056135791246 1107 4 0.07356021027145943 1107 5 0.167945741415771 1107 6 -0.04439153602513812 1107 7 0.134082582150711 1107 8 0.04827316819345384 1107 9 -0.014574549505049106 1107 10-11 0.058697805669169156 1107 12-13 0.06507001218572128 1107 14-15 -0.056199424233852824 1107 16-17 -0.07630063405778031 1107 18-19 0.016931759585020245 1107 20-21 -0.14831392901106 1107 22-23 -0.18195230796192163 1107 24-25 -0.12161351375875995 1107 26-27 -0.2790679754951313 1107 28-29 -0.1400731340884036 1107 30-31 0.11255099686444225 1107 32-33 0.14272728984332161 1107 34-35 0.06660757105600368 1107 36-37 0.09705482901244977 1107 38-39 0.15468253644425545 1107 40-41 0.002410857358036367 1107 42-43 -0.024723134502401933 1107 44-45 -0.07847888946330528 1107 46-47 -0.02255173737059124 1107 48-49 -0.014741049432807074 1107 50-51 -0.02633488068370582 1107 52-53 0.03566067256360839 1107 54-55 -0.013557052700473804 1107 56-57 -0.025119742563610714 1107 58-59 -0.08548603689905931 1107 60-61 -0.017468329062211296 1107 62-63 -0.05054196579439463 1107 64-65 -0.019663976878327105 1107 66-67 -0.07582237889143073 1107 68-69 -0.1427281271094536 1107 70-71 -0.02041973768009342 1107 72-73 -0.046256041830044836 1107 74-75 -0.24243632375126012 1107 76-77 -0.18602951591129013 1108 1 0.09136827519269275 1108 2 0.3334726034077917 1108 3 0.30839902253627116 1108 4 0.3399377802452861 1108 5 0.23343501211851958 1108 6 0.24605686414572148 1108 7 0.30835237357534595 1108 8 0.2966099636376569 1108 9 0.2747947990569344 1108 10-11 0.2935744566647358 1108 12-13 0.2942844770843678 1108 14-15 0.21000029805522757 1108 16-17 0.24573891331237974 1108 18-19 0.19876409509764414 1108 20-21 0.21982316084010733 1108 22-23 0.22367777302782343 1108 24-25 0.285307102102756 1108 26-27 0.1249508673853228 1108 28-29 0.2655209460498398 1108 30-31 0.387680043394937 1108 32-33 0.29782782787344075 1108 34-35 0.3220895789782716 1108 36-37 0.36137676775743444 1108 38-39 0.3605154744059149 1108 40-41 0.4288212559344231 1108 42-43 0.34150803200866875 1108 44-45 0.2243344292517051 1108 46-47 0.3112394618647265 1108 48-49 0.37712995829885543 1108 50-51 0.3312172979417376 1108 52-53 0.3914858190366388 1108 54-55 0.44664691038456894 1108 56-57 0.48087413833513537 1108 58-59 0.43687710073341535 1108 60-61 0.4711180444589722 1108 62-63 0.31997010196929665 1108 64-65 0.23844191919156188 1108 66-67 0.31039952131351356 1108 68-69 0.2487732370967244 1108 70-71 0.3819382659179027 1108 72-73 0.4033374094237594 1108 74-75 0.469588656317832 1108 76-77 0.5464571655128232 1109 1 -0.05011674173019287 1109 2 -0.08260333165567602 1109 3 -0.1652534690829981 1109 4 0.03564410224826986 1109 5 -0.12316060891644298 1109 6 -0.14889667190352185 1109 7 -0.11079819975806515 1109 8 -0.020791552696017845 1109 9 0.0038682642937217793 1109 10-11 -0.0728698000465684 1109 12-13 -0.05159618562555579 1109 14-15 0.20989927192026414 1109 16-17 0.16360735603015542 1109 18-19 0.07093294191996335 1109 20-21 0.08478498345382235 1109 22-23 0.031221775223229997 1109 24-25 -0.01614371928948799 1109 26-27 -0.09786449289190102 1109 28-29 0.16325330616025013 1109 30-31 0.2148011886627188 1109 32-33 0.2589280576372701 1109 34-35 0.27666023808300366 1109 36-37 0.31126645959409416 1109 38-39 0.3837572698999665 1109 40-41 0.3776498370485939 1109 42-43 0.4129645840445306 1109 44-45 0.3812038028627711 1109 46-47 0.36390959202836726 1109 48-49 0.33485233686570837 1109 50-51 0.2227323133041672 1109 52-53 0.36620157373280904 1109 54-55 0.3889636777634813 1109 56-57 0.4345756498260194 1109 58-59 0.5484914741561404 1109 60-61 0.42249891433300135 1109 62-63 0.31306911565288686 1109 64-65 0.4429648229328933 1109 66-67 0.47948417879321426 1109 68-69 0.44571258913043366 1109 70-71 0.5173544505468648 1109 72-73 0.4101622562030265 1109 74-75 0.40679317841444274 1109 76-77 0.5818446969237492 1110 1 -0.2729203286208204 1110 2 -0.21887084067965645 1110 3 -0.4432839421785175 1110 4 -0.33408193818875276 1110 5 -0.38842019068002287 1110 6 -0.30192720862888933 1110 7 -0.35414150007004963 1110 8 -0.6468824863596225 1110 9 -0.5083942245901483 1110 10-11 -0.4753149422134513 1110 12-13 -0.3638539149971365 1110 14-15 -0.403734211019561 1110 16-17 -0.4938186666414097 1110 18-19 -0.45528668946471385 1110 20-21 -0.5750971511696221 1110 22-23 -0.5098139379171513 1110 24-25 -0.4147188943860982 1110 26-27 -0.4948761554786216 1110 28-29 -0.2904837890712244 1110 30-31 -0.19420559020626627 1110 32-33 -0.2187395853657108 1110 34-35 -0.23689794530024244 1110 36-37 -0.1414243701238007 1110 38-39 -0.24801377261647417 1110 40-41 -0.23673339633498358 1110 42-43 -0.25552246880947393 1110 44-45 -0.30379500344137256 1110 46-47 -0.34157516047954317 1110 48-49 -0.3501989640591603 1110 50-51 -0.36083231450960795 1110 52-53 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-0.6730334181031168 2314 14-15 -0.6375463188983375 2314 16-17 -0.8050478467520108 2314 18-19 -0.784288673742985 2314 20-21 -0.7596007809545071 2314 22-23 -0.5816510411312379 2314 24-25 -0.7308681671583344 2314 26-27 -0.7015372493050407 2314 28-29 -0.888664805336596 2314 30-31 -0.8688429991517026 2314 32-33 -1.0284294155831475 2314 34-35 -0.987350777313516 2314 36-37 -1.5325779356567395 2314 38-39 -1.1495370672746716 2314 40-41 -1.0512957424077456 2314 42-43 -0.9261527328653969 2314 44-45 -0.8509081296187802 2314 46-47 -0.7652932677471078 2314 48-49 -0.7912412277605085 2314 50-51 -0.6453354173852297 2314 52-53 -0.5113027342815357 2314 54-55 -0.5661209582645093 2314 56-57 -0.490753244934087 2314 58-59 -0.5464334619900626 2314 60-61 -0.42313862891328213 2314 62-63 -0.516666880995885 2314 64-65 -0.5071339751964921 2314 66-67 -0.4243224915865582 2314 68-69 -0.4231799242502454 2314 70-71 -0.4072288758190439 2314 72-73 -0.3659178988040246 2314 74-75 -0.3059223991681925 2314 76-77 -0.3335944697118336 2315 1 -1.0453210858542974 2315 2 -1.0305508343845489 2315 3 -0.9592012391903708 2315 4 -0.8702984793730053 2315 5 -0.9097937196512262 2315 6 -0.9957164942626235 2315 7 -1.3311796296705793 2315 8 -1.1667628995428103 2315 9 -0.8482238885250553 2315 10-11 -1.0043645529009027 2315 12-13 -1.0372803125560424 2315 14-15 -0.8740873793025301 2315 16-17 -0.7591706530745626 2315 18-19 -0.8796577830262606 2315 20-21 -0.7747153534976619 2315 22-23 -0.8800584336677986 2315 24-25 -1.0464268529739158 2315 26-27 -0.7620323213818168 2315 28-29 -0.8457777227579655 2315 30-31 -0.8725275364140543 2315 32-33 -0.8384107549965094 2315 34-35 -0.9867784960371964 2315 36-37 -1.0453222797414625 2315 38-39 -1.0161897580956563 2315 40-41 -1.0777826109484963 2315 42-43 -1.1304467763097037 2315 44-45 -1.1732918287307932 2315 46-47 -0.9939075615826845 2315 48-49 -0.9160978379691862 2315 50-51 -0.9853264883490453 2315 52-53 -1.0822979218368722 2315 54-55 -1.087312041939061 2315 56-57 -1.2077834113774237 2315 58-59 -0.9879346228669981 2315 60-61 -1.1454287354372603 2315 62-63 -1.14189644309997 2315 64-65 -1.020227268162163 2315 66-67 -1.1541466481010332 2315 68-69 -1.2030409947173872 2315 70-71 -1.146588682411398 2315 72-73 -1.0632163733709241 2315 74-75 -1.0876198386791067 2315 76-77 -1.0349706666784826 2316 1 -2.211200621878824 2316 2 -1.8443244163264012 2316 3 -2.078458465525401 2316 4 -3.0237771958780613 2316 5 -2.3480827371479194 2316 6 -2.2963612936127547 2316 7 -1.951445681772448 2316 8 -1.8780008182506123 2316 9 -2.1013274033479874 2316 10-11 -1.9187050439393687 2316 12-13 -2.532743311880594 2316 14-15 -3.6769961348312563 2316 16-17 -2.801708874501898 2316 18-19 -2.385014168523547 2316 20-21 -2.178110502355338 2316 22-23 -2.6677516614120833 2316 24-25 -2.5058559495053174 2316 26-27 -2.492811919565945 2316 28-29 -3.0317553901793772 2316 30-31 -3.2398031278840485 2316 32-33 -3.3303054786106543 2316 34-35 -3.192394627914311 2316 36-37 -3.2762954030037434 2316 38-39 -3.339482511900968 2316 40-41 -3.041234264461412 2316 42-43 -3.201368960109157 2316 44-45 -3.0629427699888367 2316 46-47 -2.9311315179408055 2316 48-49 -2.739913652540565 2316 50-51 -2.8479835535627807 2316 52-53 -2.8985098659066892 2316 54-55 -3.076290492526656 2316 56-57 -2.8069873195786776 2316 58-59 -2.8878778040476725 2316 60-61 -2.7476967533087056 2316 62-63 -2.802736876915695 2316 64-65 -2.7937855130620477 2316 66-67 -2.6298734779385455 2316 68-69 -2.678539920949415 2316 70-71 -2.55830884299413 2316 72-73 -2.5490542826537954 2316 74-75 -2.5642574975283203 2316 76-77 -3.106391922571863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 110047.0 3 8776.0 4 10485.0 5 12937.0 6 14883.0 7 15964.0 8 17645.0 9 17955.0 10 19978.0 11 21487.0 12 18733.0 13 17580.0 14 17851.0 15 18663.0 16 19336.0 17 20294.0 18 21206.0 19 22549.0 20 24207.0 21 26033.0 22 29075.0 23 32803.0 24 37946.0 25 44350.0 26 52139.0 27 61080.0 28 71459.0 29 84712.0 30 100502.0 31 120405.0 32 146799.0 33 183908.0 34 242195.0 35 324510.0 36 461591.0 37 632110.0 38 591155.0 39 161889.0 40 1264.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.842332753011497 26.632914335582765 27.971964173489727 22.552788737916007 2 22.658759826201745 26.73873851328877 28.012795046728872 22.589706613780614 3 22.776576456802626 26.99168807537952 27.54248831155966 22.689247156258187 4 22.534697934978507 26.93287643840952 27.803658583321578 22.728767043290393 5 22.702085216827307 27.00737443286628 27.850189105401626 22.44035124490479 6 23.02099721453551 26.810949469301885 27.676411664800565 22.491641651362034 7 22.923252073808527 26.57611142554507 27.82893448775059 22.67170201289582 8 22.664582768473522 26.820656141155858 27.79266690303877 22.722094187331844 9 22.65638951536606 26.61371497068556 27.855039192371496 22.874856321576882 10-11 22.923608091211232 26.856599684852807 27.70368811899257 22.516104104943388 12-13 22.98180942199646 26.72643767641036 27.620969292523167 22.670783609070014 14-15 22.785490029654532 26.87862861349427 27.68824996559073 22.647631391260465 16-17 23.077411333740066 26.6845243673287 27.700281016361405 22.53778328256983 18-19 22.841404839645204 26.655313768810164 27.619984228465665 22.883297163078968 20-21 22.937740910961303 26.79660451180701 27.54802056937916 22.717634007852524 22-23 23.028797336770992 26.842642425790775 27.28331610594 22.845244131498234 24-25 22.985381099583876 26.889033960597565 27.333821559886445 22.791763379932114 26-27 23.016896121944676 26.98488960677211 27.209226664761886 22.788987606521324 28-29 23.085419111047738 27.03006038039414 27.176456051711874 22.70806445684625 30-31 22.983103919244783 26.876606983682098 27.25405223496101 22.886236862112117 32-33 22.918361627944865 27.143419466258266 27.179888559418686 22.75833034637818 34-35 23.057107095044934 26.954982117740723 27.268363188366617 22.719547598847726 36-37 23.058989291263046 26.9996115867074 27.337557219273638 22.60384190275591 38-39 22.925941814022973 26.94357230088568 27.441688040646994 22.68879784444435 40-41 22.81107969968247 26.89729540343584 27.379763303320786 22.911861593560907 42-43 22.813384049410505 27.000771273333562 27.237419361398192 22.948425315857744 44-45 22.86706036259583 26.895178719563607 27.397422588225723 22.84033832961484 46-47 22.876434237543002 26.72004225415268 27.361349651027833 23.042173857276484 48-49 22.850111993292916 26.81024540504241 27.29166536320655 23.047977238458124 50-51 22.807948836311315 26.956948648458738 27.181107219496415 23.053995295733532 52-53 22.85890283982984 26.887737874697056 27.237609395960906 23.0157498895122 54-55 22.82287950834076 26.85346012073517 27.2320937389682 23.09156663195587 56-57 22.861223104920708 26.99781263934958 27.159143684998167 22.98182057073155 58-59 22.976195953975655 26.992696122973864 27.133199916211304 22.89790800683918 60-61 23.001912295132186 26.877040856895928 27.084994805387797 23.03605204258409 62-63 22.81818196039415 26.817731953172135 27.332893755015654 23.031192331418065 64-65 22.91905732055317 26.827643625966534 27.191033879620154 23.062265173860148 66-67 22.934423299752886 26.872516189617347 27.21400406625676 22.97905644437301 68-69 22.913373282844994 26.713504744428203 27.315576372479004 23.0575456002478 70-71 22.762567420355893 26.804854761146572 27.347986184841766 23.084591633655762 72-73 22.87152318018983 26.613708539546906 27.340238469374405 23.17452981088886 74-75 22.730236449803083 26.55951417479299 27.284560819777866 23.42568855562606 76-77 22.803815452389145 26.482945787593753 27.435752428089856 23.277486331927246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3053.0 1 1906.5 2 816.0 3 866.5 4 861.0 5 964.0 6 1118.0 7 1235.5 8 1400.5 9 1499.0 10 1591.5 11 1781.5 12 1985.5 13 2092.0 14 2219.0 15 2545.0 16 3000.0 17 3256.0 18 3639.5 19 4553.0 20 5975.0 21 8147.0 22 9427.0 23 11325.0 24 15675.0 25 21721.5 26 25316.0 27 29802.5 28 40618.0 29 52226.0 30 57505.0 31 65390.0 32 80283.5 33 95149.5 34 109819.0 35 116631.0 36 124729.0 37 139073.5 38 147850.5 39 150381.0 40 154782.0 41 163136.5 42 171112.0 43 175134.0 44 176922.5 45 181108.5 46 184891.0 47 186746.0 48 187216.0 49 186875.0 50 183396.5 51 175969.0 52 171679.0 53 169360.0 54 159622.5 55 144132.5 56 127657.0 57 119253.0 58 111067.0 59 92832.5 60 74170.0 61 65556.0 62 58058.0 63 43140.5 64 30900.0 65 26079.0 66 22408.0 67 16704.0 68 12357.5 69 8757.0 70 7470.0 71 6285.5 72 4319.0 73 3063.5 74 2590.0 75 2291.5 76 1681.5 77 1106.5 78 843.0 79 688.0 80 462.5 81 328.0 82 215.5 83 167.0 84 144.5 85 113.0 86 97.0 87 90.0 88 81.5 89 74.0 90 69.5 91 64.0 92 55.5 93 46.0 94 44.0 95 46.0 96 49.0 97 50.0 98 44.0 99 87.0 100 137.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003232111760168966 2 0.008575522331416048 3 0.011625176169639992 4 0.019653324735220974 5 0.01420565249429102 6 0.013449755389090216 7 0.017828745515770753 8 0.019314474308751644 9 0.017672353011246443 10-11 0.021712492711457653 12-13 0.021021759149808642 14-15 0.008471260661733178 16-17 0.006451190811627575 18-19 0.012407138692261517 20-21 0.010699853851204522 22-23 0.014492372085918914 24-25 0.02215560480760985 26-27 0.013866802067821694 28-29 0.008393064409471025 30-31 0.008223639196236362 32-33 0.009800596950189769 34-35 0.011377554704143176 36-37 0.009930924037293357 38-39 0.01682522694507313 40-41 0.01288934891454479 42-43 0.01659063818828667 44-45 0.018636773455812994 46-47 0.01733350258477712 48-49 0.014518437503339632 50-51 0.027277459330780837 52-53 0.032216855932006794 54-55 0.026039352003296753 56-57 0.015626217743720125 58-59 0.017242273623804608 60-61 0.02074807226689111 62-63 0.026247875342662493 64-65 0.021308478741436532 66-67 0.02050045080139429 68-69 0.030235884208032266 70-71 0.056353432463591174 72-73 0.06754852924578933 74-75 0.07880878957153928 76-77 0.07642380387754363 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3836501.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.67808816829516 #Duplication Level Percentage of deduplicated Percentage of total 1 75.83907924247157 45.259312576345415 2 13.561043136542516 16.185942559132766 3 4.772390772744585 8.54421471928229 4 2.118854693043522 5.057967887490291 5 1.1078002621465544 3.3055700858621284 6 0.6630374051408208 2.374128283372292 7 0.4042755217446392 1.6888473161672075 8 0.2938195691750413 1.4027672123838895 9 0.20461718665680745 1.0990046255448118 >10 0.9557064576230792 9.978783992422098 >50 0.0557864833815143 2.26855800463643 >100 0.02240574899268248 2.2614375960198516 >500 0.001008184004664266 0.39011430830617705 >1k 1.7533633204447642E-4 0.18335083303433725 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.6065417420717473E-5 2.6065417420717473E-5 2 2.6065417420717473E-5 0.0 0.0 5.2130834841434946E-5 2.6065417420717473E-5 3 2.6065417420717473E-5 0.0 0.0 1.0426166968286989E-4 2.6065417420717473E-5 4 2.6065417420717473E-5 0.0 0.0 6.255700180972194E-4 2.6065417420717473E-5 5 7.819625226215242E-5 0.0 0.0 0.0011468783665115688 2.6065417420717473E-5 6 1.0426166968286989E-4 0.0 0.0 0.0025022800723888774 2.6065417420717473E-5 7 1.0426166968286989E-4 0.0 0.0 0.005108821814460624 2.6065417420717473E-5 8 1.0426166968286989E-4 0.0 0.0 0.008523391496574613 5.2130834841434946E-5 9 1.0426166968286989E-4 0.0 0.0 0.011729437839322862 5.2130834841434946E-5 10 1.3032708710358735E-4 2.6065417420717473E-5 0.0 0.015144007521436851 5.2130834841434946E-5 11 2.3458875678645724E-4 2.6065417420717473E-5 0.0 0.019627259317800256 5.2130834841434946E-5 12 2.3458875678645724E-4 2.6065417420717473E-5 0.0 0.02463181946257801 5.2130834841434946E-5 13 2.3458875678645724E-4 2.6065417420717473E-5 0.0 0.03234718301911038 5.2130834841434946E-5 14 2.3458875678645724E-4 2.6065417420717473E-5 0.0 0.042121714551879436 5.2130834841434946E-5 15 2.3458875678645724E-4 2.6065417420717473E-5 0.0 0.0534080402950501 5.2130834841434946E-5 16 2.3458875678645724E-4 2.6065417420717473E-5 0.0 0.0641469922723857 5.2130834841434946E-5 17 2.3458875678645724E-4 2.6065417420717473E-5 0.0 0.07426037423162407 5.2130834841434946E-5 18 2.606541742071747E-4 2.6065417420717473E-5 0.0 0.0850253916263804 5.2130834841434946E-5 19 3.388504264693271E-4 2.6065417420717473E-5 0.0 0.09740646490122119 1.3032708710358735E-4 20 3.388504264693271E-4 2.6065417420717473E-5 0.0 0.11096048195999428 1.3032708710358735E-4 21 3.388504264693271E-4 2.6065417420717473E-5 0.0 0.12495761111491956 1.3032708710358735E-4 22 4.1704667873147957E-4 2.6065417420717473E-5 0.0 0.14049259989766716 1.3032708710358735E-4 23 4.43112096152197E-4 2.6065417420717473E-5 0.0 0.15670528953335344 1.5639250452430484E-4 24 4.43112096152197E-4 2.6065417420717473E-5 0.0 0.17385633419618554 1.5639250452430484E-4 25 4.43112096152197E-4 2.6065417420717473E-5 0.0 0.1908249209370726 1.5639250452430484E-4 26 4.43112096152197E-4 2.6065417420717473E-5 0.0 0.20873186270510552 1.824579219450223E-4 27 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.2274989632480221 1.824579219450223E-4 28 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.24710015714840164 1.824579219450223E-4 29 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.26696200522298835 1.824579219450223E-4 30 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.2883877783428181 1.824579219450223E-4 31 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.3099960093845929 1.824579219450223E-4 32 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.33410652049875655 1.824579219450223E-4 33 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.36097996585951625 1.824579219450223E-4 34 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.38863537374289747 1.824579219450223E-4 35 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.4183499496025154 1.824579219450223E-4 36 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.44996730093384574 1.824579219450223E-4 37 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.4817410447697003 1.824579219450223E-4 38 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.5134626577707134 2.0852333936573978E-4 39 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.5466699995647075 2.0852333936573978E-4 40 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.583891415641492 2.0852333936573978E-4 41 4.43112096152197E-4 5.2130834841434946E-5 0.0 0.6233023267816169 2.0852333936573978E-4 42 5.213083484143494E-4 5.2130834841434946E-5 0.0 0.6671964897181051 2.0852333936573978E-4 43 5.213083484143494E-4 5.2130834841434946E-5 0.0 0.7106996713932826 2.3458875678645724E-4 44 5.213083484143494E-4 5.2130834841434946E-5 0.0 0.7604846186668529 2.3458875678645724E-4 45 5.213083484143494E-4 5.2130834841434946E-5 0.0 0.8097221921745883 2.3458875678645724E-4 46 5.213083484143494E-4 5.2130834841434946E-5 0.0 0.8673006992569532 2.3458875678645724E-4 47 5.213083484143494E-4 5.2130834841434946E-5 0.0 0.9215949637443076 2.606541742071747E-4 48 5.213083484143494E-4 5.2130834841434946E-5 0.0 0.9803985454454462 2.606541742071747E-4 49 5.213083484143494E-4 5.2130834841434946E-5 0.0 1.0411570334531386 2.867195916278922E-4 50 5.213083484143494E-4 5.2130834841434946E-5 0.0 1.1055646798997316 2.867195916278922E-4 51 5.213083484143494E-4 5.2130834841434946E-5 0.0 1.172761326010341 2.867195916278922E-4 52 5.213083484143494E-4 5.2130834841434946E-5 0.0 1.2433986072204855 2.867195916278922E-4 53 5.213083484143494E-4 5.2130834841434946E-5 0.0 1.3199527381851328 2.867195916278922E-4 54 5.213083484143494E-4 5.2130834841434946E-5 0.0 1.4026322422436486 2.867195916278922E-4 55 5.213083484143494E-4 5.2130834841434946E-5 0.0 1.4926621940148068 2.867195916278922E-4 56 5.995046006765019E-4 5.2130834841434946E-5 0.0 1.5855854071196644 2.867195916278922E-4 57 5.995046006765019E-4 5.2130834841434946E-5 0.0 1.68278334868152 3.909812613107621E-4 58 6.255700180972194E-4 5.2130834841434946E-5 0.0 1.786002401667561 3.909812613107621E-4 59 6.255700180972194E-4 5.2130834841434946E-5 0.0 1.888882604227133 3.909812613107621E-4 60 6.255700180972194E-4 5.2130834841434946E-5 0.0 1.9925187038919057 3.909812613107621E-4 61 6.777008529386542E-4 5.2130834841434946E-5 0.0 2.102462634572492 3.909812613107621E-4 62 6.777008529386542E-4 5.2130834841434946E-5 0.0 2.219261770034727 3.909812613107621E-4 63 6.777008529386542E-4 5.2130834841434946E-5 0.0 2.3418213627469404 4.43112096152197E-4 64 6.777008529386542E-4 5.2130834841434946E-5 0.0 2.472304842355052 4.43112096152197E-4 65 6.777008529386542E-4 5.2130834841434946E-5 0.0 2.610894666781007 4.43112096152197E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 150 5.569673E-7 21.303427 52 TCGCGCT 390 0.0 20.932987 37 ATTCGCG 395 0.0 20.669899 56 CGATCGT 400 0.0 19.52076 1 CTCGCGC 460 0.0 19.290545 36 CGCGGAA 395 0.0 17.976871 59 CCTCGCG 495 0.0 17.9254 35 AACGCGA 205 5.751954E-7 17.318956 53 AATTCGC 495 0.0 16.495237 55 ACGCGAA 195 6.8550853E-6 16.384901 54 CGCGCTT 505 0.0 16.166912 38 GACGTAA 440 0.0 16.132017 31 ACGATCG 320 1.1332304E-9 15.533141 59 CAATTCG 535 0.0 15.261948 54 ATCGTTC 610 0.0 15.128256 3 TCGCGGA 495 0.0 15.062048 58 GTCGGGT 590 0.0 15.0487175 66 GTACGAC 190 1.00614016E-4 14.946849 19 AGCGCGA 390 8.0035534E-11 14.5637865 23 TAATTCG 470 0.0 14.347947 35 >>END_MODULE