FastQCFastQC Report
Thu 26 May 2016
SRR936176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3836501
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54900.1430991416397389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38570.10053431499170729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12100.039.35555100-101
GGTATCA71800.033.1558721
CGTACAT18450.031.295128100-101
GTATCAA131000.029.3939461
CGGTATC9900.027.958838100-101
TCGAGTA6250.026.667318100-101
CCTGTCG10050.026.05588796-97
CCGTACA9200.025.880552100-101
ACGTATC5350.023.92137100-101
ATCAACG162950.023.185073
TCAACGC163250.023.1786064
TACGCAG9700.023.012346100-101
CGGACTA2500.022.619602100-101
TATCAAC169100.022.5186522
CAACGCA169550.022.2813935
GCGTACA8300.022.232328100-101
TCGTATC6400.021.856936100-101
TCGTACA11500.021.739662100-101
TCGTATA5950.021.50913100-101
CGCGTAC1951.2732926E-1121.364798-99
Produced by FastQC (version 0.11.5)