FastQCFastQC Report
Thu 26 May 2016
SRR936175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306387
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86060.260284110722671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG5200.028.67220356
AATTCGC5400.026.95125255
CAATTCG5550.026.2252254
CGGTTAC4200.025.3525334
CGATCGT6100.024.4400311
CGCTCCG6450.023.66451525
CGCGGAA5800.023.2575659
TCGCGCT6550.022.76163337
CTCGCGC6500.022.38993336
CGCGCTT6900.022.12017338
TCGCGGA6300.021.41107658
ATCGTTC7250.021.0510273
CCTCGCG7100.020.99872635
AACTCGA7500.019.40431413
ACGCTCC8450.019.32485424
GATCGTT8050.018.9595742
ACTCGAG8150.018.7277914
GCTCCGC8550.018.6825126
GACGCTC8500.018.37618823
CGTTCGG1401.6770003E-417.74731311
Produced by FastQC (version 0.11.5)