##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936175_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3306387 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.51976764970344 39.0 36.0 41.0 30.0 41.0 2 36.37806312449208 39.0 36.0 41.0 29.0 41.0 3 36.289853244644384 39.0 36.0 41.0 29.0 41.0 4 36.16536358266591 39.0 36.0 41.0 29.0 41.0 5 36.08828639841616 39.0 35.0 41.0 29.0 41.0 6 35.987053239684286 39.0 35.0 40.0 29.0 41.0 7 35.81750986802211 39.0 35.0 40.0 28.0 41.0 8 35.63790657294503 39.0 35.0 40.0 27.0 41.0 9 35.537212975976495 38.0 35.0 40.0 27.0 41.0 10-11 35.749080038120155 39.0 35.0 40.0 27.5 41.0 12-13 35.91782329170783 39.0 35.0 41.0 28.5 41.0 14-15 35.857880217893424 39.0 35.0 41.0 28.0 41.0 16-17 35.685688184716426 39.0 35.0 41.0 27.0 41.0 18-19 35.52350057630882 39.0 35.0 41.0 27.0 41.0 20-21 35.30899513577812 39.0 35.0 41.0 26.0 41.0 22-23 35.03588675493825 39.0 34.5 41.0 24.5 41.0 24-25 34.79327858475127 39.0 34.0 41.0 24.0 41.0 26-27 33.84945576546242 38.0 33.0 39.5 22.5 40.5 28-29 33.87600407937728 38.0 33.0 39.5 23.0 40.5 30-31 34.238565539968555 38.0 34.0 40.0 22.5 41.0 32-33 34.085000787869056 38.0 33.5 40.0 22.0 41.0 34-35 33.816790200300204 38.0 33.0 40.0 20.5 41.0 36-37 33.52253759163703 37.5 33.0 40.0 19.5 41.0 38-39 33.24124036297022 37.0 33.0 40.0 18.5 41.0 40-41 32.862776045272376 36.5 32.0 40.0 17.0 41.0 42-43 32.506836918969256 36.0 32.0 39.0 13.5 41.0 44-45 32.13868264664723 35.5 31.5 39.0 11.5 40.5 46-47 31.75113469778341 35.0 31.0 38.5 8.0 40.0 48-49 31.29058410282886 35.0 31.0 37.5 7.0 40.0 50-51 30.853115954061032 35.0 30.5 37.0 2.0 39.0 52-53 30.404382638813907 35.0 30.0 36.5 2.0 39.0 54-55 29.9445075546208 34.5 30.0 36.0 2.0 38.5 56-57 29.498519985712502 34.0 29.0 35.5 2.0 37.0 58-59 29.07735119936051 34.0 29.0 35.0 2.0 37.0 60-61 28.73047377696561 34.0 29.0 35.0 2.0 36.5 62-63 28.390607330599835 34.0 29.0 35.0 2.0 36.0 64-65 28.021050923560974 34.0 27.5 35.0 2.0 36.0 66-67 27.648226901448623 34.0 27.0 35.0 2.0 35.0 68-69 27.2872062465767 33.5 26.5 35.0 2.0 35.0 70-71 26.876134735589027 33.0 25.5 35.0 2.0 35.0 72-73 26.397963698744277 33.0 25.0 35.0 2.0 35.0 74-75 25.82082269861332 33.0 23.5 35.0 2.0 35.0 76-77 24.129390782143773 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8978664923791726 1101 2 0.9042378905507391 1101 3 0.8759056817864561 1101 4 0.979621836928672 1101 5 1.1140700095798408 1101 6 0.9287591347106243 1101 7 1.042144710164834 1101 8 1.0311505995818564 1101 9 1.1278572984295252 1101 10-11 1.0000266691052815 1101 12-13 1.094680559948408 1101 14-15 0.9976730677173151 1101 16-17 1.0897241859724716 1101 18-19 1.1360920718720777 1101 20-21 1.1112447195257928 1101 22-23 1.1592319051623363 1101 24-25 1.1577769519252783 1101 26-27 0.9883123749574168 1101 28-29 1.0701999153540669 1101 30-31 1.0269416645396845 1101 32-33 1.2388932587030226 1101 34-35 1.2213093902287326 1101 36-37 1.2141825255962928 1101 38-39 1.223202844986929 1101 40-41 1.1177662415536176 1101 42-43 1.0599263099497591 1101 44-45 1.0173700194140807 1101 46-47 0.9822072716096812 1101 48-49 1.1497904618527492 1101 50-51 1.118367586724883 1101 52-53 1.1286408415042004 1101 54-55 1.2401190656891465 1101 56-57 1.2141138839107555 1101 58-59 1.0822265738108179 1101 60-61 1.0208890134283024 1101 62-63 1.008158895935491 1101 64-65 1.1118759059325463 1101 66-67 1.0449128503852343 1101 68-69 0.8503818955341949 1101 70-71 0.9623632540922316 1101 72-73 0.945357220915156 1101 74-75 0.917532780892337 1101 76-77 0.9288179783450765 1102 1 0.8267662783145013 1102 2 0.9612557124816945 1102 3 0.8856388592706921 1102 4 0.9203760718021954 1102 5 0.9232123052698142 1102 6 0.848120680118285 1102 7 0.7239802563537552 1102 8 0.7948996021580186 1102 9 0.7387979009989252 1102 10-11 0.7078690765153439 1102 12-13 0.8656928747757959 1102 14-15 0.7562290617165672 1102 16-17 0.8061121731628162 1102 18-19 0.9376943653898664 1102 20-21 0.8663313523725265 1102 22-23 0.7872992962998424 1102 24-25 0.8881372688318478 1102 26-27 0.7754724341295542 1102 28-29 0.7909864703659508 1102 30-31 0.9260260528665896 1102 32-33 0.9103662533684656 1102 34-35 0.8850511055426225 1102 36-37 0.9603792366836856 1102 38-39 0.9962560238102114 1102 40-41 1.0165883637873776 1102 42-43 0.9589045647376366 1102 44-45 0.9616715471665529 1102 46-47 0.6611827019106755 1102 48-49 0.6795339556030804 1102 50-51 0.8322476577633147 1102 52-53 0.8491997906659137 1102 54-55 0.9632798473443636 1102 56-57 0.9490111405094694 1102 58-59 0.9702071442689046 1102 60-61 0.8046902044274482 1102 62-63 0.834714196299327 1102 64-65 0.9458404790738939 1102 66-67 0.9028715149239197 1102 68-69 0.8393353962348797 1102 70-71 0.8549922798067939 1102 72-73 0.9893105531584503 1102 74-75 1.0034247380260126 1102 76-77 1.3348305402074203 1103 1 0.5783239480690625 1103 2 0.37818197276929766 1103 3 0.4635959147293036 1103 4 0.5088328952145815 1103 5 0.5972335843847034 1103 6 0.5608295109250392 1103 7 0.5847520844718801 1103 8 0.707250619402032 1103 9 0.6307159942962031 1103 10-11 0.686578078439986 1103 12-13 0.6284837751929544 1103 14-15 0.5958906782448281 1103 16-17 0.6210841461261936 1103 18-19 0.6889900648861058 1103 20-21 0.7423467706995979 1103 22-23 0.7482149541919583 1103 24-25 0.6932271294917669 1103 26-27 0.5559969385574419 1103 28-29 0.5328030596406634 1103 30-31 0.7246154321493918 1103 32-33 0.6644614156582307 1103 34-35 0.7616282205122431 1103 36-37 0.7698254736888828 1103 38-39 0.7097944631113222 1103 40-41 0.775365123644157 1103 42-43 0.8733608505978552 1103 44-45 0.8729874307960088 1103 46-47 0.7031797751897528 1103 48-49 0.7401498763080845 1103 50-51 0.8113718150293003 1103 52-53 0.841048572574433 1103 54-55 0.7918315482821363 1103 56-57 0.8801051844833125 1103 58-59 0.6455134820262387 1103 60-61 0.6809782878811248 1103 62-63 0.6678125126029748 1103 64-65 0.6024025477682216 1103 66-67 0.5077770997625315 1103 68-69 0.4592158492133258 1103 70-71 0.5151707263847385 1103 72-73 0.6164507691472565 1103 74-75 0.4508093349958422 1103 76-77 0.7091756145616017 1104 1 0.5018180467688609 1104 2 0.43326259972558745 1104 3 0.41543916554888227 1104 4 0.34722562901759346 1104 5 0.5470287319424827 1104 6 0.3287364108809001 1104 7 0.4872248016980194 1104 8 0.6976544785794587 1104 9 0.5120136064849206 1104 10-11 0.38927291568673894 1104 12-13 0.5005670285058059 1104 14-15 0.4862383967344499 1104 16-17 0.6325426584527136 1104 18-19 0.49117184447431583 1104 20-21 0.4533748991636415 1104 22-23 0.4127311756078029 1104 24-25 0.5623757367983941 1104 26-27 0.4174538925903093 1104 28-29 0.4899449300647518 1104 30-31 0.6639165248080587 1104 32-33 0.5839157433345363 1104 34-35 0.6262769390052298 1104 36-37 0.48646682861258483 1104 38-39 0.6204492749536854 1104 40-41 0.7468186578541776 1104 42-43 0.6635888331719215 1104 44-45 0.5425466274240307 1104 46-47 0.5622230586913872 1104 48-49 0.6032365106970197 1104 50-51 0.6082794023190168 1104 52-53 0.6421964032611953 1104 54-55 0.6574571484357783 1104 56-57 0.5891966066242205 1104 58-59 0.6464343493472207 1104 60-61 0.524294597909126 1104 62-63 0.6772690387368208 1104 64-65 0.6044906612726457 1104 66-67 0.6008942008983169 1104 68-69 0.46044795410586303 1104 70-71 0.42285867344655514 1104 72-73 0.3669610050308023 1104 74-75 0.4769815087565341 1104 76-77 0.4437638796837895 1105 1 0.2817721981214589 1105 2 0.40437006095028494 1105 3 0.4275203980282072 1105 4 0.5423760526050785 1105 5 0.40600427987286736 1105 6 0.194907332946336 1105 7 0.31495477301413644 1105 8 0.4464338274896136 1105 9 0.2796262065652968 1105 10-11 0.44967399373194183 1105 12-13 0.45947997790145934 1105 14-15 0.3531827503384761 1105 16-17 0.38864225316889645 1105 18-19 0.4665129434426376 1105 20-21 0.48530543960172423 1105 22-23 0.41050300943981455 1105 24-25 0.3597858902075117 1105 26-27 0.18303966681907013 1105 28-29 0.28704819202192766 1105 30-31 0.3255954427279164 1105 32-33 0.4486732157041331 1105 34-35 0.4795907565640576 1105 36-37 0.5142985683068062 1105 38-39 0.45857755842783376 1105 40-41 0.5459389837082256 1105 42-43 0.4617352006672135 1105 44-45 0.3852862142655695 1105 46-47 0.2847523079482528 1105 48-49 0.27835357280420325 1105 50-51 0.3783670646725241 1105 52-53 0.3437543298870871 1105 54-55 0.36397821554758636 1105 56-57 0.4644502140732456 1105 58-59 0.5848722088108644 1105 60-61 0.24397478000211947 1105 62-63 0.06149022185319453 1105 64-65 0.22951201554862877 1105 66-67 0.25157281945156384 1105 68-69 0.07800283662887253 1105 70-71 -0.1262945990047193 1105 72-73 -0.3531602953917279 1105 74-75 -0.48177626623366265 1105 76-77 -0.42605923369643506 1106 1 0.18358341611974538 1106 2 0.21453541559668565 1106 3 -0.027284451741991234 1106 4 0.12771219080617868 1106 5 0.013517733501316798 1106 6 0.14005469896439848 1106 7 0.39528093298815037 1106 8 0.2646924284431691 1106 9 0.20004994779087326 1106 10-11 0.10976787661644494 1106 12-13 0.08422643111016015 1106 14-15 0.18279767014037418 1106 16-17 0.46118609150116185 1106 18-19 0.3522259346115604 1106 20-21 0.12574489479625583 1106 22-23 0.1906570767026352 1106 24-25 0.16481863548411724 1106 26-27 0.06069633398485763 1106 28-29 0.2168910073302186 1106 30-31 0.20255713189980895 1106 32-33 0.21431323776342737 1106 34-35 0.10618291586221318 1106 36-37 0.17964738487025755 1106 38-39 0.10862397211636932 1106 40-41 0.24469287128932393 1106 42-43 0.17022941705932482 1106 44-45 0.13209350888551086 1106 46-47 0.31606068303140233 1106 48-49 0.11018413768067958 1106 50-51 0.09852197879594726 1106 52-53 0.03075270816949427 1106 54-55 0.15384151915488786 1106 56-57 0.09179984313193756 1106 58-59 -0.1072517216696518 1106 60-61 -0.06553425767785725 1106 62-63 -0.08921608852653051 1106 64-65 -0.07922635852811055 1106 66-67 -0.0010728017063712514 1106 68-69 -0.14409576576475658 1106 70-71 -0.24464214077703872 1106 72-73 -0.13273516318738032 1106 74-75 -0.38480745443226994 1106 76-77 -0.4709317995858733 1107 1 0.23578747172529546 1107 2 0.19034830843611417 1107 3 0.06920004776308986 1107 4 0.21690480704233295 1107 5 0.12732480842454663 1107 6 -0.12047897208216085 1107 7 -0.006581350212556458 1107 8 0.17709595037372594 1107 9 0.027015512556694432 1107 10-11 0.1271636063063113 1107 12-13 0.24059846000307772 1107 14-15 0.2646074425222622 1107 16-17 0.25353405575478405 1107 18-19 0.29900548473306543 1107 20-21 0.1956565378896684 1107 22-23 0.1868292633594919 1107 24-25 -0.10960489995154177 1107 26-27 -0.055602042059554435 1107 28-29 0.06981169781158059 1107 30-31 0.25890981126527635 1107 32-33 0.33569833352280654 1107 34-35 0.3688611100725083 1107 36-37 0.4302458253933068 1107 38-39 0.3611329311652085 1107 40-41 0.38831414524401 1107 42-43 0.38171356934129363 1107 44-45 0.3710584417250402 1107 46-47 0.32925446894451227 1107 48-49 0.37598328404600423 1107 50-51 0.3094080367887386 1107 52-53 0.3092908869832627 1107 54-55 0.3364774649184419 1107 56-57 0.2727865285598625 1107 58-59 0.27331416099821837 1107 60-61 0.37960157185003496 1107 62-63 0.42739547089699315 1107 64-65 0.3823592402794205 1107 66-67 0.44634802301481 1107 68-69 0.24808947845971474 1107 70-71 0.18373611755577102 1107 72-73 0.20192258066959212 1107 74-75 0.1891987010124936 1107 76-77 0.3914670927031416 1108 1 0.0685565462567368 1108 2 0.10051157693076362 1108 3 0.1307901879564639 1108 4 0.06005408023613512 1108 5 0.14410594932098775 1108 6 0.10506710905332994 1108 7 -0.08987291998175095 1108 8 0.16646577124108575 1108 9 -0.0014065458161525157 1108 10-11 -0.20550812006121788 1108 12-13 0.016585311991811125 1108 14-15 0.09868711177887235 1108 16-17 0.05128182786393154 1108 18-19 -0.004773667136078075 1108 20-21 -0.19137031698344487 1108 22-23 -0.13709074289001677 1108 24-25 -0.14409739753285322 1108 26-27 -0.3156490335806552 1108 28-29 -0.14860393077512413 1108 30-31 0.006676512184071726 1108 32-33 -0.023899173883464186 1108 34-35 -0.012402372948649543 1108 36-37 0.03736846605239208 1108 38-39 -0.0020021604442206353 1108 40-41 0.06319291072507127 1108 42-43 0.08846845027006367 1108 44-45 -0.05091460124636171 1108 46-47 -0.08668599243770814 1108 48-49 -0.04116982298289429 1108 50-51 -0.07211323859239016 1108 52-53 -0.08248499591050518 1108 54-55 6.118774943821848E-4 1108 56-57 -0.031725138196939895 1108 58-59 -0.05497389342060899 1108 60-61 -0.11269770765336773 1108 62-63 -0.14078670237072188 1108 64-65 -0.13637727669942024 1108 66-67 -0.15113594758844684 1108 68-69 -0.07127799884073838 1108 70-71 -0.06214070697116014 1108 72-73 -0.1100175922736284 1108 74-75 -0.20592247341498293 1108 76-77 -0.0820746378106989 1109 1 0.13253187683257295 1109 2 0.07250632102944365 1109 3 -0.020308647047095008 1109 4 0.11177196418065449 1109 5 0.0714264575148249 1109 6 0.16900866491116062 1109 7 0.015671685779388156 1109 8 0.32082363519837287 1109 9 0.29689315159939156 1109 10-11 0.2628167464592437 1109 12-13 0.28801652723392124 1109 14-15 0.3974270371968913 1109 16-17 0.4068988410684682 1109 18-19 0.3264894610505422 1109 20-21 0.3409460734707963 1109 22-23 0.37266506127213717 1109 24-25 0.19368101255699344 1109 26-27 0.20714080439323368 1109 28-29 0.4314689856023719 1109 30-31 0.5716868296664188 1109 32-33 0.5614398224984782 1109 34-35 0.4293475738446091 1109 36-37 0.4458880230021194 1109 38-39 0.4425591846223611 1109 40-41 0.46742770516195975 1109 42-43 0.42123293407208706 1109 44-45 0.4501141396506583 1109 46-47 0.25252622383152357 1109 48-49 0.3528714839598912 1109 50-51 0.375251176734853 1109 52-53 0.38330699809332813 1109 54-55 0.5680736380362568 1109 56-57 0.514037522695638 1109 58-59 0.5830464917037119 1109 60-61 0.3144229282366666 1109 62-63 0.39124676698682137 1109 64-65 0.3840129369603673 1109 66-67 0.4842224808230249 1109 68-69 0.4639791054167297 1109 70-71 0.6517973788813975 1109 72-73 0.606215555067422 1109 74-75 0.5474041049747491 1109 76-77 0.5714687002418408 1110 1 -0.27843059021821404 1110 2 -0.3871381773260083 1110 3 -0.39218809373351604 1110 4 -0.28613130822167676 1110 5 -0.06077450534698414 1110 6 -0.4556483850998774 1110 7 -0.4401393090761374 1110 8 -0.4045703647693273 1110 9 -0.3730012673549581 1110 10-11 -0.3611137609721595 1110 12-13 -0.32164510740613395 1110 14-15 -0.35825821680590764 1110 16-17 -0.43011566825062175 1110 18-19 -0.29446736268975826 1110 20-21 -0.4589155801896325 1110 22-23 -0.5298669817856947 1110 24-25 -0.4130643584031972 1110 26-27 -0.44392240786163484 1110 28-29 -0.3236201320494416 1110 30-31 -0.33862167728767645 1110 32-33 -0.16055706605465758 1110 34-35 0.05527077996271146 1110 36-37 0.012092757640921548 1110 38-39 -0.04435946019360415 1110 40-41 0.046158346094891556 1110 42-43 0.07275694191709192 1110 44-45 -0.10013837382062007 1110 46-47 -0.1849391222945087 1110 48-49 -0.005586663132230996 1110 50-51 -0.03154811520463241 1110 52-53 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-0.44366073645132786 2314 16-17 -0.9478112359350064 2314 18-19 -0.6433096409661729 2314 20-21 -0.5068763795483306 2314 22-23 -0.36385417553579913 2314 24-25 -0.34976427967033175 2314 26-27 -0.35941115381336175 2314 28-29 -0.3419811417993941 2314 30-31 -0.4676961739533141 2314 32-33 -0.538298500047496 2314 34-35 -0.5722660386946572 2314 36-37 -0.5557678094368228 2314 38-39 -0.5617110789766642 2314 40-41 -0.45825035189466234 2314 42-43 -0.32596809357823986 2314 44-45 -0.25551196466725656 2314 46-47 -0.2671271199376619 2314 48-49 -0.33055886531311174 2314 50-51 -0.21874699699085554 2314 52-53 -0.14204896135682077 2314 54-55 -0.14025118101573852 2314 56-57 -0.022354117061212264 2314 58-59 -0.021099924252524715 2314 60-61 -0.013336284762530681 2314 62-63 0.011890032658957495 2314 64-65 -0.06171664321525938 2314 66-67 -0.019449986490723603 2314 68-69 0.0774908793399085 2314 70-71 0.18678095959756646 2314 72-73 0.10589982364378514 2314 74-75 0.047296856274932964 2314 76-77 0.07530719604172376 2315 1 -1.4941454465264314 2315 2 -1.3799065984198648 2315 3 -1.281145618608889 2315 4 -1.1871258263458415 2315 5 -1.2168242232709048 2315 6 -1.3558155312562263 2315 7 -1.2974654254145648 2315 8 -1.2310699284543034 2315 9 -1.1670700638574445 2315 10-11 -1.172386351984585 2315 12-13 -1.297934013919651 2315 14-15 -1.5201039061731052 2315 16-17 -1.4227338189556775 2315 18-19 -1.3193613789537508 2315 20-21 -1.3394321338039745 2315 22-23 -1.2435809187749953 2315 24-25 -1.4185388543817723 2315 26-27 -1.4223959769908276 2315 28-29 -1.4365144986305651 2315 30-31 -1.5199747297909738 2315 32-33 -1.5216321480840023 2315 34-35 -1.3879554405671968 2315 36-37 -1.5261567982876798 2315 38-39 -1.4710154617165614 2315 40-41 -1.5751030802531147 2315 42-43 -1.6666792493072684 2315 44-45 -1.6312242756191608 2315 46-47 -1.5588108330259303 2315 48-49 -1.451435630961793 2315 50-51 -1.3802849854659023 2315 52-53 -1.3874269552657417 2315 54-55 -1.4419029530437122 2315 56-57 -1.5059199242473014 2315 58-59 -1.3604580858300466 2315 60-61 -1.4251480977753523 2315 62-63 -1.503894855399814 2315 64-65 -1.5840278849107037 2315 66-67 -1.409970622233086 2315 68-69 -1.4539285604517396 2315 70-71 -1.4253267989632121 2315 72-73 -2.895138434558099 2315 74-75 -3.418466258258402 2315 76-77 -3.0317267279107796 2316 1 -3.2328152845268576 2316 2 -3.2793673676540607 2316 3 -2.6770947375755156 2316 4 -2.6527363972602203 2316 5 -3.2824700890197533 2316 6 -2.917246525025014 2316 7 -3.250551457130875 2316 8 -3.21449183324593 2316 9 -2.8876379614593333 2316 10-11 -2.7693064132712806 2316 12-13 -2.7932395037153626 2316 14-15 -2.892425696980993 2316 16-17 -4.1359107181521395 2316 18-19 -3.885241565251036 2316 20-21 -3.223589731555805 2316 22-23 -3.289658436634184 2316 24-25 -3.4111767234357124 2316 26-27 -2.9901301080617273 2316 28-29 -3.2853645557710465 2316 30-31 -3.533530992729311 2316 32-33 -3.5729390229233147 2316 34-35 -3.5296041254445143 2316 36-37 -3.543366485270795 2316 38-39 -3.599695969902733 2316 40-41 -3.441494558668282 2316 42-43 -3.714177922964538 2316 44-45 -3.620260288239109 2316 46-47 -3.2274955097178157 2316 48-49 -3.3736961202150724 2316 50-51 -3.8065154377004724 2316 52-53 -4.874796605869172 2316 54-55 -3.917366875778491 2316 56-57 -3.9756602880144456 2316 58-59 -4.092239895392492 2316 60-61 -3.8101107521252366 2316 62-63 -3.7814891371907997 2316 64-65 -3.819852624880653 2316 66-67 -3.804646489334285 2316 68-69 -3.8134436763005333 2316 70-71 -3.7286931088176765 2316 72-73 -3.646947982145196 2316 74-75 -3.665868409831507 2316 76-77 -3.5909435466796076 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85600.0 3 7627.0 4 9565.0 5 11596.0 6 13457.0 7 14033.0 8 16346.0 9 17155.0 10 20981.0 11 23069.0 12 18410.0 13 15671.0 14 15475.0 15 16159.0 16 16168.0 17 17450.0 18 18076.0 19 19392.0 20 20252.0 21 22135.0 22 24801.0 23 27751.0 24 32142.0 25 37775.0 26 45052.0 27 52944.0 28 62275.0 29 73245.0 30 87911.0 31 105536.0 32 128387.0 33 162629.0 34 216538.0 35 288360.0 36 402561.0 37 542098.0 38 486417.0 39 130242.0 40 1106.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.771326387771424 26.501198393753107 28.604255416969643 22.123219801505826 2 22.36432278377823 26.69499536139806 28.579807395930917 22.36087445889279 3 22.634336509542084 26.909014792495796 28.07385993606041 22.382788761901708 4 22.174778168125783 26.86024486052436 28.558157853184113 22.406819118165743 5 22.550122777437988 26.7711076466841 28.445526926643883 22.233242649234025 6 22.591536174370177 26.663349535853747 28.46512626774388 22.279988022032203 7 22.46489194204845 26.407581676807734 28.52118885677425 22.606337524369568 8 22.376848788225836 26.791404477081894 28.25285996126824 22.578886773424035 9 22.34652790570837 26.522424481121078 28.490765856310173 22.640281756860382 10-11 22.891809361494623 26.697352653041595 28.12343123415353 22.287406751310257 12-13 22.98902572139559 26.372011694967114 28.049030413055092 22.589932170582205 14-15 22.543366150961088 26.711295615746412 28.194561650257853 22.550776583034647 16-17 22.80904721720641 26.482876163341107 28.314459759059467 22.393616860393017 18-19 22.736892064681875 26.489477261610585 28.063721152953214 22.709909520754326 20-21 22.637434674750555 26.762029064905292 28.02997152922531 22.570564731118843 22-23 22.814587846949756 26.846577072956173 27.555344676438366 22.783490403655705 24-25 22.912579479422355 26.869145626553987 27.487088256927162 22.731186637096492 26-27 22.82143102935827 26.95771075234581 27.373637834268987 22.847220384026937 28-29 23.094474536104066 26.894901171394416 27.374816721502132 22.635807570999386 30-31 22.766382062819332 26.8411483444622 27.498641910931138 22.89382768178733 32-33 22.81986168020933 27.192229845151346 27.335709896554317 22.652198578085013 34-35 22.94028691163235 26.76177756173963 27.446229968809742 22.851705557818274 36-37 22.87759647866193 26.911061531462643 27.687630670873947 22.523711319001478 38-39 22.797315119543125 26.870689550866324 27.676425926822183 22.655569402768368 40-41 22.5898888223267 26.786074289752815 27.619721571094136 23.00431531682635 42-43 22.618827236115422 27.169870904863803 27.336888758214773 22.874413100806006 44-45 22.74557566492447 26.81228574790448 27.631211590401268 22.81092699676979 46-47 22.56543610126727 26.877751823885692 27.64695036389985 22.909861710947187 48-49 22.784127176198325 26.91370834782872 27.43065660308865 22.871507872884305 50-51 22.604553474193736 27.103371939267852 27.32285712254304 22.969217463995367 52-53 22.652262589448096 26.975891107427273 27.40073080354806 22.971115499576566 54-55 22.69033480568431 26.983321204501188 27.38229448663275 22.944049503181756 56-57 22.628723356465574 27.234218071886968 27.289939947729735 22.847118623917723 58-59 22.76366335703721 27.174529702345097 27.212961474303874 22.84884546631382 60-61 22.70020048597619 27.040997907413445 27.13713437400504 23.12166723260533 62-63 22.755324671552984 26.939098898944685 27.296043388767277 23.009533040735054 64-65 22.683792476712078 26.902799996127758 27.264143979748155 23.149263547412012 66-67 22.626240929848418 26.997581292911633 27.443900063076974 22.932277714162975 68-69 22.7394737363635 26.761026179749837 27.434025671891344 23.065474411995325 70-71 22.550985470201983 26.612347930207708 27.52238749063263 23.314279108957674 72-73 22.747945316605282 26.527194588324747 27.435259916844046 23.289600178225925 74-75 22.556160087369808 26.569604576259927 27.326810564711817 23.547424771658452 76-77 22.423843767930677 26.47429417365681 27.67166794585717 23.430194112555345 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8272.0 1 5016.0 2 1720.0 3 1761.0 4 1842.0 5 1890.5 6 2002.5 7 2199.0 8 2415.0 9 2498.0 10 2571.5 11 2742.5 12 2974.0 13 3108.0 14 3333.5 15 3701.0 16 4194.0 17 4545.0 18 4954.0 19 6047.0 20 7680.0 21 10077.5 22 11526.0 23 13409.5 24 17335.0 25 22357.0 26 25337.0 27 28430.0 28 36723.0 29 45174.0 30 48425.0 31 55012.0 32 68037.0 33 80335.0 34 91533.5 35 96872.0 36 103580.0 37 114889.0 38 122160.0 39 124830.0 40 128116.0 41 135984.5 42 146228.5 43 151890.0 44 150763.0 45 148605.5 46 151418.0 47 156699.0 48 158137.0 49 159474.0 50 158622.0 51 151886.5 52 147340.0 53 145688.5 54 137393.5 55 121665.0 56 105020.5 57 97461.0 58 93023.0 59 80001.0 60 65848.5 61 60280.0 62 53026.0 63 38935.0 64 28015.5 65 23933.0 66 20958.5 67 16212.0 68 12380.0 69 8803.0 70 7286.0 71 6320.5 72 4624.0 73 3589.0 74 3285.0 75 2828.5 76 1886.0 77 1101.0 78 802.0 79 687.5 80 484.0 81 337.0 82 244.0 83 209.0 84 176.5 85 136.0 86 103.0 87 78.0 88 81.5 89 77.0 90 56.0 91 43.0 92 44.5 93 48.5 94 45.5 95 41.0 96 42.0 97 49.5 98 55.5 99 120.5 100 187.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00892212557090262 2 0.013126110161938091 3 0.013731000031151828 4 0.015273469197646857 5 0.012249019851578172 6 0.00958750442703773 7 0.015787625586478535 8 0.017330094752973563 9 0.01687642735106326 10-11 0.019462331541951986 12-13 0.015742258846287504 14-15 0.007167944950182782 16-17 0.005353275342541572 18-19 0.016513493429535016 20-21 0.021670179564582125 22-23 0.01962867625598576 24-25 0.017496439467007338 26-27 0.022970692783391658 28-29 0.008604558389565408 30-31 0.008196257727846136 32-33 0.010661183944892114 34-35 0.011689496722555466 36-37 0.01122070707391482 38-39 0.014124178446140757 40-41 0.01126607381410585 42-43 0.013700755537691142 44-45 0.012400242318881606 46-47 0.022335558420717237 48-49 0.022290191680526206 50-51 0.03485677871344159 52-53 0.03804757277354405 54-55 0.027295655348269877 56-57 0.02067211128037946 58-59 0.015984214793973 60-61 0.020248688371929842 62-63 0.025133174065830766 64-65 0.024528284196617033 66-67 0.027280533101539536 68-69 0.04030078753636522 70-71 0.05127953866259455 72-73 0.08211379974576478 74-75 0.08252210040748406 76-77 0.08666559601159815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3306387.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.185686625563505 #Duplication Level Percentage of deduplicated Percentage of total 1 73.21849949001853 36.74520670600051 2 13.723936405018197 13.774903433828117 3 5.2796509326495045 7.948887215949364 4 2.551390068530802 5.121730497554473 5 1.4215426688861177 3.5670547452792944 6 0.8873421832434735 2.6719126042740187 7 0.6070291911100897 2.1324923730334184 8 0.4072934967966767 1.6352243035894374 9 0.2953935157562285 1.3342073771668987 >10 1.4182979987758253 12.808574999056821 >50 0.11528030302489994 4.032658571250348 >100 0.07046405768730288 6.345507514266583 >500 0.002788526112217595 0.907202823871523 >1k 0.0010305422574463168 0.7126196821796218 >5k 6.0620132790959813E-5 0.26181715269970224 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8606 0.260284110722671 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.1171145422480792E-4 0.0 3 0.0 0.0 0.0 3.93178414988929E-4 0.0 4 0.0 0.0 0.0 7.561123365171712E-4 0.0 5 0.0 0.0 0.0 0.0016332026468770897 0.0 6 0.0 0.0 0.0 0.0032059163068328058 0.0 7 0.0 0.0 0.0 0.007349411910946904 0.0 8 0.0 0.0 0.0 0.01188608593004993 0.0 9 0.0 0.0 0.0 0.01572713659955716 0.0 10 0.0 0.0 0.0 0.02111065643555942 0.0 11 0.0 0.0 0.0 0.027189799621157473 0.0 12 0.0 0.0 0.0 0.0351743458947788 0.0 13 0.0 0.0 0.0 0.04603211904716538 0.0 14 0.0 0.0 0.0 0.05961189661101377 0.0 15 0.0 0.0 0.0 0.07461316536751444 0.0 16 0.0 0.0 0.0 0.08997736804554336 0.0 17 0.0 0.0 0.0 0.10476692534781924 0.0 18 0.0 0.0 0.0 0.1193447711958703 0.0 19 0.0 0.0 0.0 0.1347694628608206 0.0 20 0.0 0.0 0.0 0.15318835937837888 0.0 21 0.0 0.0 0.0 0.1715165224155551 0.0 22 0.0 0.0 0.0 0.1926876678380359 0.0 23 0.0 0.0 0.0 0.21446370312973043 0.0 24 0.0 0.0 0.0 0.23620949392796428 0.0 25 0.0 0.0 0.0 0.25901384199732214 0.0 26 0.0 0.0 0.0 0.28393530460892813 0.0 27 0.0 0.0 0.0 0.3089777451943768 0.0 28 0.0 0.0 0.0 0.3345948311555786 3.0244493460686847E-5 29 0.0 0.0 0.0 0.36136120786828646 3.0244493460686847E-5 30 0.0 0.0 0.0 0.3892466308390397 3.0244493460686847E-5 31 0.0 0.0 0.0 0.41924916835204107 3.0244493460686847E-5 32 0.0 0.0 0.0 0.45288104508032484 3.0244493460686847E-5 33 0.0 0.0 0.0 0.48820661344240707 3.0244493460686847E-5 34 0.0 0.0 0.0 0.5257400298271194 3.0244493460686847E-5 35 0.0 0.0 0.0 0.5650578713260124 3.0244493460686847E-5 36 0.0 0.0 0.0 0.6070372282494457 3.0244493460686847E-5 37 0.0 0.0 0.0 0.6512546776889698 3.0244493460686847E-5 38 0.0 0.0 0.0 0.693445746066628 3.0244493460686847E-5 39 0.0 0.0 0.0 0.7402037329568498 3.0244493460686847E-5 40 0.0 0.0 0.0 0.7866895194059256 3.0244493460686847E-5 41 0.0 0.0 0.0 0.8410086296613192 3.0244493460686847E-5 42 0.0 0.0 0.0 0.8945111385932741 3.0244493460686847E-5 43 0.0 0.0 0.0 0.9532761893873887 3.0244493460686847E-5 44 0.0 0.0 0.0 1.0185740507690115 3.0244493460686847E-5 45 0.0 0.0 0.0 1.0865031830817142 3.0244493460686847E-5 46 0.0 0.0 0.0 1.1583943440377669 3.0244493460686847E-5 47 0.0 0.0 0.0 1.2310416173303367 3.0244493460686847E-5 48 0.0 0.0 0.0 1.3061991835801434 3.0244493460686847E-5 49 0.0 0.0 0.0 1.3840485097479516 3.0244493460686847E-5 50 0.0 0.0 0.0 1.4651339967160528 3.0244493460686847E-5 51 0.0 0.0 0.0 1.5540225629970115 3.0244493460686847E-5 52 0.0 0.0 0.0 1.648143426646669 3.0244493460686847E-5 53 0.0 0.0 0.0 1.7477990325996322 3.0244493460686847E-5 54 0.0 0.0 0.0 1.857526054875004 3.0244493460686847E-5 55 0.0 0.0 0.0 1.972182929584468 3.0244493460686847E-5 56 0.0 0.0 0.0 2.093765793296429 3.0244493460686847E-5 57 0.0 0.0 0.0 2.2174657715506383 6.048898692137369E-5 58 0.0 0.0 0.0 2.3518723004899305 6.048898692137369E-5 59 0.0 0.0 0.0 2.486006628988077 6.048898692137369E-5 60 0.0 0.0 0.0 2.6169955301663115 6.048898692137369E-5 61 0.0 0.0 0.0 2.7615642089083945 6.048898692137369E-5 62 0.0 0.0 0.0 2.911697874447244 6.048898692137369E-5 63 0.0 0.0 0.0 3.074322515785357 6.048898692137369E-5 64 0.0 0.0 0.0 3.2401228289368427 6.048898692137369E-5 65 0.0 0.0 0.0 3.420954655338289 6.048898692137369E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCGCG 520 0.0 28.672203 56 AATTCGC 540 0.0 26.951252 55 CAATTCG 555 0.0 26.22522 54 CGGTTAC 420 0.0 25.352533 4 CGATCGT 610 0.0 24.440031 1 CGCTCCG 645 0.0 23.664515 25 CGCGGAA 580 0.0 23.25756 59 TCGCGCT 655 0.0 22.761633 37 CTCGCGC 650 0.0 22.389933 36 CGCGCTT 690 0.0 22.120173 38 TCGCGGA 630 0.0 21.411076 58 ATCGTTC 725 0.0 21.051027 3 CCTCGCG 710 0.0 20.998726 35 AACTCGA 750 0.0 19.404314 13 ACGCTCC 845 0.0 19.324854 24 GATCGTT 805 0.0 18.959574 2 ACTCGAG 815 0.0 18.72779 14 GCTCCGC 855 0.0 18.68251 26 GACGCTC 850 0.0 18.376188 23 CGTTCGG 140 1.6770003E-4 17.747313 11 >>END_MODULE