FastQCFastQC Report
Thu 26 May 2016
SRR936175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306387
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96670.29237351828445973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70250.2124675665613251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48580.1469277492320167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13650.045.566914100-101
GGTATCA85750.038.0268941
CGTACAT20950.037.218018100-101
CGGTATC12200.034.88287100-101
TACGCAG11000.034.630062100-101
TCGTATC5600.033.480236100-101
GTATCAA176700.031.8902451
ACGTATC4950.030.06082100-101
CCGTACA10700.029.482079100-101
AGTATCA37350.029.481335100-101
CGAGTAC14550.029.044327100-101
GCGTATC4650.028.800205100-101
TCGGTAT5550.028.41966100-101
ACCGTAC3000.027.77577698-99
AACGTAT3350.027.53888598-99
AGGTATC27750.026.918245100-101
CCTGTCG7750.026.8759296-97
GCGTACA7550.026.409723100-101
GACGCAG11050.025.855024100-101
TCGAGTA6450.025.376925100-101
Produced by FastQC (version 0.11.5)