FastQCFastQC Report
Thu 26 May 2016
SRR936174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319577
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85860.2586474120046018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG4850.025.6178556
CGATCGT5050.023.8967291
CGGTTAC3900.021.8425884
CAATTCG5400.021.69482854
CTCGCGC5600.021.55070536
TCGCGCT5500.021.2987737
TCGCGGA5600.020.9206458
CGCGGAA5700.020.55423259
AATTCGC5750.020.3733555
AACTCGA6750.019.45784413
CGCTCCG6300.019.15560325
CGCGATA950.006648733318.6831320
GACGTAT6000.017.75031339
TATGCGA1000.00893677217.74897623
ACTCGAG7800.017.2925714
CGCGCTT7000.017.24264138
CGCGTAC1250.001601886217.04312562
GCGAACG1250.001605606917.0372225
ATACGTA2501.4368197E-817.0369631
TCCGCGT3601.8189894E-1216.7629240
Produced by FastQC (version 0.11.5)