FastQCFastQC Report
Thu 26 May 2016
SRR936174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319577
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98020.29527858519323397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70350.21192459159706192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48860.14718742779577038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14400.047.742947100-101
GGTATCA89000.037.446891
CGTACAT20200.036.539284100-101
TCGTATC5850.035.103706100-101
CGGTATC11650.034.998898100-101
TACGCAG10800.034.170998100-101
GTATCAA177550.031.6088541
CGAGTAC14250.030.492832100-101
GCGTATC4350.030.103926100-101
CCTGTCG7850.029.56870796-97
AGGTATC26800.028.096062100-101
CCGTATC7350.027.939686100-101
AGTATCA37350.027.80959100-101
ACGTATC5550.027.348715100-101
TCGAGTA6650.026.852785100-101
GGGTATC17700.026.567062100-101
GCGTACA6450.026.301159100-101
CCGTACA9550.026.17795100-101
TATACGT4450.026.08026596-97
TCGTACA11650.026.057573100-101
Produced by FastQC (version 0.11.5)