FastQCFastQC Report
Thu 26 May 2016
SRR936173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399350
Sequences flagged as poor quality0
Sequence length77
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6646616.643545761863027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5638714.119694503568297No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13070.3272818329785902No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7480.18730436960060098No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6220.15575309878552648No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG4430.11093026167522224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGA252.2289617E-456.79208416
TCTAGCG252.2303418E-456.7849734
CGAATGC200.00657328653.24258419
CAATACG200.00657328653.24258414
AATACGA200.00657328653.24258415
CTAGCGG200.00657654353.2359125
TACCGAG305.481801E-447.32673617
TCTACCG400.002256582535.49505615
CGGCCGT400.002257969735.4906129
ATAGTTG450.00401175831.56301560
GGACATC500.006719101728.40315853
GCATCTC500.006731443628.3924873
CTCGAAC657.318486E-427.30730623
GAGTACT958.417459E-626.1509746
CGAACTC750.001686038723.66633225
GGGTAAT1003.5750703E-421.2943653
GGCCTCG850.00348801220.88205725
GGAACTC1205.1209405E-520.7054481
TAAGAGG900.00485628119.72194323
ATCTAGT900.00485980119.71947515