##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936173_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 399350 Sequences flagged as poor quality 0 Sequence length 77 %GC 24 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.73649430324277 39.0 35.0 41.0 31.0 41.0 2 36.49608864404657 39.0 35.0 41.0 31.0 41.0 3 36.322504069112306 39.0 35.0 41.0 31.0 41.0 4 36.13179166144985 39.0 35.0 41.0 31.0 41.0 5 35.91076499311381 38.0 35.0 40.0 31.0 41.0 6 35.56259171153124 38.0 35.0 40.0 30.0 41.0 7 35.044129209966194 37.0 35.0 40.0 30.0 41.0 8 34.56532615500188 35.0 35.0 40.0 29.0 41.0 9 34.24693376737198 35.0 34.0 40.0 28.0 41.0 10-11 34.07630274195568 35.0 34.0 40.0 27.0 41.0 12-13 33.71686615750595 35.0 33.5 40.0 24.0 41.0 14-15 33.37404782772005 35.0 33.0 40.0 22.0 41.0 16-17 32.93355327407036 35.0 33.0 39.5 20.5 41.0 18-19 32.42485664204332 35.0 33.0 39.0 16.5 41.0 20-21 31.715810692375108 35.0 33.0 39.0 4.5 41.0 22-23 30.775288593965193 35.0 31.5 39.0 2.0 41.0 24-25 30.117295605358706 35.0 30.5 39.0 2.0 41.0 26-27 29.11940778765494 34.0 27.5 38.0 2.0 40.0 28-29 29.195284837861525 35.0 27.5 38.0 2.0 40.0 30-31 29.49520721171904 35.0 29.5 38.0 2.0 41.0 32-33 29.32691749092275 35.0 29.0 38.0 2.0 41.0 34-35 29.09322899712032 35.0 28.0 38.0 2.0 41.0 36-37 28.864074120445725 35.0 27.0 37.0 2.0 40.0 38-39 28.593933892575436 35.0 27.0 37.0 2.0 40.0 40-41 28.257296857393264 35.0 26.0 36.0 2.0 40.0 42-43 27.964507324402156 35.0 26.0 36.0 2.0 40.0 44-45 27.65896832352573 35.0 25.5 35.0 2.0 40.0 46-47 27.34304369600601 35.0 24.0 35.0 2.0 39.0 48-49 26.936971328408667 35.0 22.5 35.0 2.0 39.0 50-51 26.5620245398773 34.5 20.0 35.0 2.0 38.5 52-53 26.157091523726052 34.0 15.5 35.0 2.0 37.5 54-55 25.724166771002878 34.0 4.5 35.0 2.0 37.0 56-57 25.26513960185301 33.0 2.0 35.0 2.0 36.5 58-59 24.797871541254537 33.0 2.0 35.0 2.0 36.0 60-61 24.380830098910728 33.0 2.0 35.0 2.0 35.5 62-63 23.947592337548514 33.0 2.0 35.0 2.0 35.0 64-65 23.484966821084264 33.0 2.0 35.0 2.0 35.0 66-67 22.969659446600726 33.0 2.0 35.0 2.0 35.0 68-69 22.490659822211093 32.5 2.0 35.0 2.0 35.0 70-71 21.993768624014024 31.5 2.0 35.0 2.0 35.0 72-73 21.472124702641793 31.0 2.0 35.0 2.0 35.0 74-75 20.944280706147488 31.0 2.0 35.0 2.0 35.0 76-77 20.16241517465882 28.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.2962930802383212 1101 2 1.246135612273541 1101 3 1.4994262473167481 1101 4 1.3826354628028454 1101 5 1.3940134105991575 1101 6 1.1828862121769461 1101 7 1.156644219033609 1101 8 1.221799599191172 1101 9 1.2661612612776452 1101 10-11 0.9892422584964535 1101 12-13 0.9861597726848714 1101 14-15 1.4252309340417142 1101 16-17 1.6975952943429107 1101 18-19 1.260370475317437 1101 20-21 1.3558552232497583 1101 22-23 1.2484908355786004 1101 24-25 0.6842105141925359 1101 26-27 0.6975299400526893 1101 28-29 0.601923866758959 1101 30-31 0.7086592899084323 1101 32-33 0.9259739401541864 1101 34-35 0.7650681000486692 1101 36-37 0.8793000957616002 1101 38-39 0.9653723958065399 1101 40-41 0.6369467412869732 1101 42-43 0.511577354964988 1101 44-45 0.8865415494287525 1101 46-47 0.9310654994290068 1101 48-49 1.0858469226098748 1101 50-51 0.986912303819846 1101 52-53 1.0735462124266704 1101 54-55 0.8884800421369263 1101 56-57 0.9748741543319923 1101 58-59 1.1061581555120164 1101 60-61 1.0646861531938754 1101 62-63 0.6185564642120305 1101 64-65 0.9750561378895846 1101 66-67 1.0617763934920461 1101 68-69 1.415397376715827 1101 70-71 1.344721668195234 1101 72-73 1.2492201589277414 1101 74-75 0.9027440508548068 1101 76-77 0.9232327341102398 1102 1 0.19454889419181143 1102 2 0.562996077389819 1102 3 0.4790774101074433 1102 4 0.27798430001215024 1102 5 0.2893622478084623 1102 6 0.6916071424095023 1102 7 0.2264116608940725 1102 8 0.2014507619818744 1102 9 0.05685893569624767 1102 10-11 -0.04709495080586379 1102 12-13 0.44255512152207643 1102 14-15 0.6069169805533434 1102 16-17 0.9010836664359303 1102 18-19 1.0699634985732516 1102 20-21 0.8994598744125462 1102 22-23 0.9621536262762724 1102 24-25 1.0257802816343968 1102 26-27 1.1568322656340868 1102 28-29 0.984191308619426 1102 30-31 1.3307523131642434 1102 32-33 1.4172530099216303 1102 34-35 1.02088205353704 1102 36-37 1.0740675376220636 1102 38-39 0.974093326039096 1102 40-41 0.6209583691939535 1102 42-43 0.9548913084533588 1102 44-45 1.0347973633822392 1102 46-47 0.9659492203592386 1102 48-49 0.7326492481912652 1102 50-51 0.7281913735872863 1102 52-53 0.7639531891708558 1102 54-55 0.6515614374857677 1102 56-57 0.6202229915412971 1102 58-59 0.7602279229538773 1102 60-61 0.8393954555194547 1102 62-63 0.9165215804911 1102 64-65 0.6872654402151674 1102 66-67 0.8175903469804204 1102 68-69 0.6566764464832708 1102 70-71 0.3519891100557011 1102 72-73 0.871313182183556 1102 74-75 0.47541846945945565 1102 76-77 0.6165466875986105 1103 1 0.6410588466078693 1103 2 0.6282732035877956 1103 3 0.8003444163402662 1103 4 0.484312629212603 1103 5 0.5812746531432254 1103 6 0.8963353777824707 1103 7 1.0707334221701288 1103 8 0.7963170578065473 1103 9 0.8948892285720618 1103 10-11 0.802119243953193 1103 12-13 0.9705902733215552 1103 14-15 1.1625097692404296 1103 16-17 1.2041297089263807 1103 18-19 1.177253559561123 1103 20-21 1.2478823997124557 1103 22-23 1.1987580763801517 1103 24-25 0.9089859984094097 1103 26-27 0.6409988764510501 1103 28-29 0.5713796673085234 1103 30-31 0.892213508794562 1103 32-33 0.707322375245667 1103 34-35 0.9403118839773583 1103 36-37 0.7558876234071974 1103 38-39 0.8188716585905311 1103 40-41 0.8651150946144988 1103 42-43 1.1182935603131519 1103 44-45 1.020044665841862 1103 46-47 0.8088677579907646 1103 48-49 0.725151426329461 1103 50-51 1.0885140731382528 1103 52-53 1.107181692622369 1103 54-55 1.0513377594480993 1103 56-57 0.805239076254118 1103 58-59 0.8543234929558885 1103 60-61 0.9595867965823857 1103 62-63 0.9073775888015341 1103 64-65 1.1637666800784494 1103 66-67 1.41003974715467 1103 68-69 1.4619969678960452 1103 70-71 1.3212061531493227 1103 72-73 1.1404682321131858 1103 74-75 1.0535172899138487 1103 76-77 0.9077176882017888 1104 1 -0.02638133836632761 1104 2 0.452530961110746 1104 3 0.15930996824697985 1104 4 0.17333313722145505 1104 5 -0.09435868242410095 1104 6 -0.20664867154398792 1104 7 -0.1689371763152323 1104 8 0.0037763433772184385 1104 9 0.22546358685903556 1104 10-11 0.2668585375662289 1104 12-13 0.24778767966161297 1104 14-15 0.2769751200882311 1104 16-17 0.2978859920173278 1104 18-19 0.5815914055499931 1104 20-21 0.6770761534823144 1104 22-23 1.101688509997203 1104 24-25 1.3571756304716018 1104 26-27 1.0260183121457125 1104 28-29 1.1077378202473334 1104 30-31 1.1694151038619154 1104 32-33 1.0756832424797693 1104 34-35 1.1298936814440168 1104 36-37 1.1845326539011332 1104 38-39 1.1296165818530497 1104 40-41 0.4290979040776719 1104 42-43 0.7688447968254515 1104 44-45 0.4519485261729379 1104 46-47 0.20432131338248993 1104 48-49 0.3794515737726627 1104 50-51 0.20493555963379606 1104 52-53 0.2116276077755046 1104 54-55 0.2925498095787873 1104 56-57 0.05772299154129712 1104 58-59 0.15557676016317856 1104 60-61 0.19259312993806077 1104 62-63 0.13454483630505365 1104 64-65 -0.19209502490111063 1104 66-67 -0.4004329088335332 1104 68-69 -0.272102623284173 1104 70-71 -0.47359228529314024 1104 72-73 -0.46880309688621224 1104 74-75 -0.646674553796359 1104 76-77 -0.7003137775176675 1105 1 0.10995424692664102 1105 2 0.17018069477321518 1105 3 0.3261119690264991 1105 4 0.36303366989652375 1105 5 -0.09694085729742596 1105 6 0.16234249384513078 1105 7 0.44495459970139706 1105 8 0.7934996121832469 1105 9 0.2813944690209169 1105 10-11 0.5059614347989125 1105 12-13 0.7704832103878658 1105 14-15 0.6991607118772336 1105 16-17 0.9869288009039039 1105 18-19 1.2382271077716354 1105 20-21 1.4908347607318966 1105 22-23 1.2158076473234374 1105 24-25 1.2242098645887936 1105 26-27 1.181582168193522 1105 28-29 1.1183263612373295 1105 30-31 0.9355739969371406 1105 32-33 1.2386282210428448 1105 34-35 1.3948020873164317 1105 36-37 1.5515181677376937 1105 38-39 1.8171409419933617 1105 40-41 1.4925723096902495 1105 42-43 1.2899081981506448 1105 44-45 0.8647148637070394 1105 46-47 1.0254448030537908 1105 48-49 1.0837438304960614 1105 50-51 1.2742320387815163 1105 52-53 1.0669364943546924 1105 54-55 1.2480357131775932 1105 56-57 1.304401892411768 1105 58-59 1.2612672889406262 1105 60-61 1.1377097338097002 1105 62-63 1.015756672085228 1105 64-65 0.9231527989263562 1105 66-67 0.9815746915302448 1105 68-69 1.0628655461324819 1105 70-71 1.0013898506039638 1105 72-73 1.4449214061669267 1105 74-75 1.1876196517382667 1105 76-77 0.8246553663046008 1106 1 -0.03928308908491118 1106 2 0.4298630750381065 1106 3 -0.004297295928616052 1106 4 0.017826212737524827 1106 5 0.19996454725972512 1106 6 0.4813902623467925 1106 7 0.4087672919366625 1106 8 0.7314350839050476 1106 9 0.4955837854480123 1106 10-11 0.5672570220188007 1106 12-13 0.6670292526953219 1106 14-15 0.405127066782768 1106 16-17 0.5994766635616173 1106 18-19 1.0247910397281998 1106 20-21 0.5921859000200733 1106 22-23 0.8523417631981474 1106 24-25 1.2656385256897913 1106 26-27 1.2987515235384492 1106 28-29 1.082554909351071 1106 30-31 0.7557751770262762 1106 32-33 0.8852762657355839 1106 34-35 1.0677039767144656 1106 36-37 1.0765009057955695 1106 38-39 1.12452120688571 1106 40-41 1.0574458267324935 1106 42-43 0.8897293016595249 1106 44-45 0.7076154976910978 1106 46-47 0.9349686873046252 1106 48-49 0.8789779652019831 1106 50-51 0.9631012246455555 1106 52-53 0.6976643206053978 1106 54-55 1.0501275467096995 1106 56-57 1.3387688499160006 1106 58-59 1.1973196397032844 1106 60-61 1.0481751785400988 1106 62-63 0.6140163544654946 1106 64-65 0.7023882518169451 1106 66-67 0.7005110420418283 1106 68-69 0.442589041204986 1106 70-71 0.21652072751062335 1106 72-73 0.2844795004485121 1106 74-75 0.17317454471422167 1106 76-77 3.819371413342765E-4 1107 1 0.31595263430209286 1107 2 0.371974739581141 1107 3 0.34256937846035385 1107 4 0.41820846687861035 1107 5 0.3142064098799082 1107 6 -0.0018716396571463179 1107 7 0.20588301069028603 1107 8 0.4904701817852768 1107 9 0.598995114070739 1107 10-11 0.5760170127520325 1107 12-13 0.5564217002801684 1107 14-15 0.4185928376619543 1107 16-17 0.17119275298592385 1107 18-19 0.2581977349673963 1107 20-21 0.14491959630178286 1107 22-23 -0.048605184560457815 1107 24-25 0.001970044281243588 1107 26-27 -0.33177957804848646 1107 28-29 -0.3223448937301292 1107 30-31 -0.23807111047517182 1107 32-33 -0.4742598167386447 1107 34-35 -0.11884762759458667 1107 36-37 -0.22144013439903532 1107 38-39 -0.2754121882260705 1107 40-41 -0.07166630114889116 1107 42-43 0.20216414470365507 1107 44-45 0.03458758155533914 1107 46-47 0.13921402496244895 1107 48-49 -0.058138332724578135 1107 50-51 -0.16922531305860034 1107 52-53 -0.35983307311636636 1107 54-55 -0.08358900605295361 1107 56-57 0.10589788964726665 1107 58-59 0.035701430506019705 1107 60-61 0.09547912849955509 1107 62-63 -0.10204411118955292 1107 64-65 -0.2588505991039405 1107 66-67 -0.1269822974693504 1107 68-69 -0.01760729842202835 1107 70-71 0.38464015297106613 1107 72-73 0.3592507271368035 1107 74-75 0.21503426902790324 1107 76-77 0.32625359241760066 1108 1 0.0685299538897226 1108 2 0.11166546123062204 1108 3 0.19566216196550812 1108 4 0.20429094110541257 1108 5 0.03843321400361788 1108 6 -0.012180918007665298 1108 7 0.13629538182430423 1108 8 0.15799595498115337 1108 9 0.36188171200888064 1108 10-11 0.009006703473680489 1108 12-13 0.24070005079563828 1108 14-15 0.17761345621865843 1108 16-17 0.053924711748805976 1108 18-19 -0.04592597637279994 1108 20-21 0.36141444166260683 1108 22-23 0.13180718657356394 1108 24-25 -0.15524645056412112 1108 26-27 -0.2802331862959093 1108 28-29 -0.16641705867858647 1108 30-31 0.04156806478255959 1108 32-33 0.1739360595500159 1108 34-35 0.34893587756005573 1108 36-37 0.16515780374529498 1108 38-39 0.4836084303306336 1108 40-41 0.4863233895727568 1108 42-43 0.5114424952191214 1108 44-45 0.3657731485656477 1108 46-47 0.6263274270243038 1108 48-49 0.7704699146981007 1108 50-51 0.6104138621991275 1108 52-53 0.5203215660588896 1108 54-55 0.2566171795140555 1108 56-57 0.23862984841015233 1108 58-59 0.43647462638231005 1108 60-61 0.327437891386154 1108 62-63 0.5126466104599317 1108 64-65 0.39449991635997605 1108 66-67 0.19775997057188377 1108 68-69 0.23368136137178652 1108 70-71 0.12561953441436557 1108 72-73 -0.13043999451268107 1108 74-75 -0.41769768973498245 1108 76-77 -0.4095711498504393 1109 1 0.06133916459405242 1109 2 0.5149584774386469 1109 3 0.28463042237626013 1109 4 0.4766277108648609 1109 5 0.4685952942590461 1109 6 0.28522552732069073 1109 7 0.11726651043322533 1109 8 0.35410900528285083 1109 9 0.39585362531353496 1109 10-11 0.33616055795321387 1109 12-13 0.24827980564586483 1109 14-15 0.30092906625193194 1109 16-17 0.672569352818158 1109 18-19 0.4522881643853722 1109 20-21 0.3381272430263529 1109 22-23 0.16273502162511022 1109 24-25 -0.20127291375106537 1109 26-27 -0.5354554716545685 1109 28-29 -0.04781976993761461 1109 30-31 0.0014437310974670936 1109 32-33 -0.1193169101174334 1109 34-35 -0.021498005060283987 1109 36-37 0.05340969107381355 1109 38-39 0.12641967042046787 1109 40-41 0.16096554126255924 1109 42-43 0.2999669966667504 1109 44-45 0.2640975526027738 1109 46-47 0.23255561113015588 1109 48-49 0.45595236727812605 1109 50-51 0.5931809969773845 1109 52-53 0.7986957576869997 1109 54-55 0.8674216279270759 1109 56-57 0.9915453989147949 1109 58-59 0.7489357298268544 1109 60-61 0.7979576846594156 1109 62-63 0.40611063621959786 1109 64-65 0.5597933655035767 1109 66-67 0.4675972138546207 1109 68-69 0.6689338169282415 1109 70-71 1.0499572782788569 1109 72-73 0.7699848235190849 1109 74-75 0.8001872846947578 1109 76-77 0.9908638151088347 1110 1 -0.3209218942370029 1110 2 -0.3484049435971386 1110 3 -0.24640472262085922 1110 4 -0.29003895580180483 1110 5 -0.16805408798280297 1110 6 -0.08181883603632656 1110 7 -0.16492413037082088 1110 8 -0.156575329906957 1110 9 -0.18332824524532754 1110 10-11 -0.037579919609044055 1110 12-13 -0.10861475936277998 1110 14-15 -0.41528948569736457 1110 16-17 -0.33927367899799066 1110 18-19 -0.5421040000087132 1110 20-21 -0.8996680325641968 1110 22-23 -0.990923514960258 1110 24-25 -0.9683844943156927 1110 26-27 -1.1928557661299521 1110 28-29 -0.9009405688621364 1110 30-31 -0.8785287418556109 1110 32-33 -0.8439920269473404 1110 34-35 -0.9561244694351672 1110 36-37 -1.1051653041249594 1110 38-39 -0.9197169405519432 1110 40-41 -0.7414990896829643 1110 42-43 -0.7928823727718246 1110 44-45 -0.8435492049671645 1110 46-47 -0.8015139106674205 1110 48-49 -0.7203711715478107 1110 50-51 -0.6871659718466354 1110 52-53 -0.567250724612471 1110 54-55 -0.6316277287082208 1110 56-57 -0.48974581163511033 1110 58-59 -0.313368219984298 1110 60-61 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-0.18800135428244502 2313 60-61 -0.23115602571184724 2313 62-63 -0.02840870031561593 2313 64-65 0.3655077445671857 2313 66-67 0.5586221427924443 2313 68-69 0.6671041701154259 2313 70-71 0.41348795208985933 2313 72-73 0.4973565576539798 2313 74-75 0.502624635627555 2313 76-77 0.5567365457477997 2314 1 -0.2662761334936832 2314 2 -0.2497115638061942 2314 3 -0.18246744039089435 2314 4 -0.031308797071645245 2314 5 -0.6068171541848955 2314 6 -0.3087158550065183 2314 7 -0.6394982689693478 2314 8 -0.31179213207497725 2314 9 -0.3720816327792065 2314 10-11 -0.6685419792296372 2314 12-13 -0.5696726377987034 2314 14-15 -0.6295171279737914 2314 16-17 -1.1983764664544339 2314 18-19 -0.9566607170044534 2314 20-21 -0.8284378738340372 2314 22-23 -0.9830983117082255 2314 24-25 -0.5092187955158458 2314 26-27 -0.2965868927269355 2314 28-29 -0.4617758379291139 2314 30-31 -0.6901963763890961 2314 32-33 -0.32702532156600483 2314 34-35 -0.2855040668025701 2314 36-37 -0.2892584129131883 2314 38-39 -0.3578557326541514 2314 40-41 -0.3188026127181942 2314 42-43 -0.45344663912877436 2314 44-45 -0.44639957275655817 2314 46-47 -0.3856796094672674 2314 48-49 -0.7020591312845532 2314 50-51 -0.9288084418427651 2314 52-53 -0.7948784830329103 2314 54-55 -0.6423662110930479 2314 56-57 -0.9190673369932192 2314 58-59 -0.5633924166548354 2314 60-61 -0.5134330789951278 2314 62-63 -0.14113345826859103 2314 64-65 -0.22024194258291274 2314 66-67 -0.2886988741343828 2314 68-69 -0.1846626085185754 2314 70-71 -0.5359538578291314 2314 72-73 -0.7275701696067713 2314 74-75 -0.6846036789347849 2314 76-77 -0.9199520204117242 2315 1 -0.9982137361209382 2315 2 -1.0157576374080506 2315 3 -0.9147914044592795 2315 4 -1.151499402049268 2315 5 -1.2097945520405915 2315 6 -1.2569635448870642 2315 7 -1.3713111090479089 2315 8 -1.2634768289422311 2315 9 -1.0197602444537637 2315 10-11 -1.1029289524097194 2315 12-13 -0.9191747949834195 2315 14-15 -1.3898281489338942 2315 16-17 -1.1764081963877864 2315 18-19 -1.0171101841476329 2315 20-21 -1.5491609331727147 2315 22-23 -1.5725590960219478 2315 24-25 -1.236298707940584 2315 26-27 -1.234400811397851 2315 28-29 -1.2014748784648717 2315 30-31 -1.2539480264744185 2315 32-33 -1.3231415841629968 2315 34-35 -1.2940060308065604 2315 36-37 -1.4643747238053635 2315 38-39 -1.4183514826782968 2315 40-41 -1.4773275169327498 2315 42-43 -1.363643763955718 2315 44-45 -1.4464458556375241 2315 46-47 -1.465141279192629 2315 48-49 -1.3715832627762303 2315 50-51 -1.5944830450173662 2315 52-53 -1.5261459900357117 2315 54-55 -1.4467244486409392 2315 56-57 -1.4265068070341087 2315 58-59 -1.2160917147648824 2315 60-61 -1.247654641183221 2315 62-63 -1.1570225725349879 2315 64-65 -0.9658490934892541 2315 66-67 -0.9688117238719798 2315 68-69 -0.870715765413518 2315 70-71 -0.8390664821892209 2315 72-73 -1.9933767796070967 2315 74-75 -2.4323277685706373 2315 76-77 -2.2766823562242102 2316 1 -3.4865538232835718 2316 2 -3.1142586021337237 2316 3 -2.602724013086494 2316 4 -2.884052259571206 2316 5 -3.5248168374284816 2316 6 -2.597118170847857 2316 7 -3.322130338796537 2316 8 -3.288726105960169 2316 9 -2.8121530789482136 2316 10-11 -3.1202850193873175 2316 12-13 -3.0220293935091185 2316 14-15 -2.946220749555458 2316 16-17 -3.986723789862733 2316 18-19 -3.8693767272814377 2316 20-21 -3.207650294204992 2316 22-23 -3.3139833819225615 2316 24-25 -3.1334620996371996 2316 26-27 -3.0880885820944783 2316 28-29 -3.1002753803920733 2316 30-31 -3.2542493626981823 2316 32-33 -3.712839033599227 2316 34-35 -3.4144393253109975 2316 36-37 -3.394300559113873 2316 38-39 -3.4059697121705668 2316 40-41 -3.058793989904693 2316 42-43 -3.2764743905101845 2316 44-45 -3.1006445486478746 2316 46-47 -2.8986314272971363 2316 48-49 -2.874805761348256 2316 50-51 -3.1155473465694747 2316 52-53 -4.151618795565906 2316 54-55 -3.5348428063135415 2316 56-57 -3.2852003570896535 2316 58-59 -3.0533903929332986 2316 60-61 -2.767711490285734 2316 62-63 -2.484444618652919 2316 64-65 -2.416567128237368 2316 66-67 -2.3094948126132486 2316 68-69 -2.461397729765892 2316 70-71 -2.179378522595261 2316 72-73 -2.1682597307231646 2316 74-75 -1.9523486372285248 2316 76-77 -1.838820584097359 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10254.0 3 1856.0 4 3209.0 5 3764.0 6 4031.0 7 3720.0 8 4912.0 9 5581.0 10 8769.0 11 10729.0 12 6706.0 13 3922.0 14 3407.0 15 3198.0 16 3276.0 17 3435.0 18 3570.0 19 3780.0 20 3864.0 21 4032.0 22 4395.0 23 4913.0 24 5511.0 25 6258.0 26 7025.0 27 7740.0 28 8718.0 29 9740.0 30 11354.0 31 13120.0 32 16286.0 33 21342.0 34 48433.0 35 48397.0 36 23336.0 37 26764.0 38 28783.0 39 11015.0 40 205.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 13.407612765243437 31.664926133788125 44.04349579141654 10.883965309551895 2 13.42247087461308 31.973313832930973 43.822813467298424 10.781401825157523 3 12.408059002516874 33.29660284744744 43.44824131929227 10.847096830743416 4 12.473891679898221 33.34351787386864 43.497838706930665 10.684751739302476 5 11.94519505042486 34.19774759771303 43.28699471834954 10.57006263351256 6 12.247070019032355 34.34413502955024 43.34393468897125 10.064860262446159 7 12.288254445279238 34.714500375657394 42.703481091910845 10.293764087152518 8 11.66047649697337 35.10865563539191 42.64630380501341 10.58456406262131 9 11.622630168549176 35.01991034085501 42.67950612336898 10.677953367226827 10-11 11.38092876713701 35.590509965387874 42.574671535406054 10.453889732069065 12-13 11.414772072708331 35.89372742891203 41.9521705810756 10.73932991730404 14-15 11.6412670150119 36.2235219431203 41.57591784962555 10.559293192242244 16-17 11.426382000468294 36.659934413749326 41.244586129221695 10.669097456560692 18-19 11.127112435742506 37.05727291511437 40.78342485395313 11.032189795190002 20-21 11.346538943693105 37.41858161375281 40.02872890260351 11.206150539950583 22-23 11.307529795709819 37.866130007852014 38.90041714619903 11.92592305023913 24-25 11.523024886387685 38.46526637689109 37.62925005228232 12.382458684438902 26-27 11.81770363691013 39.00949303676986 36.535667768760646 12.637135557559361 28-29 11.505255842781747 39.27488777726997 36.1633286795595 13.05652770038879 30-31 11.483579503052704 39.13647769446907 36.11307278910543 13.2668700133728 32-33 11.631544019132765 39.11536005409263 35.601717941975636 13.651377984798968 34-35 11.578434638010354 38.85181076214542 36.03230096141565 13.537453638428579 36-37 11.986481477771322 38.890419329633566 35.93014344960231 13.192955742992806 38-39 12.147807739746181 38.84273201394932 36.19574132393361 12.813718922370883 40-41 12.070997026138675 38.94681483800282 36.26958835498513 12.712599780873376 42-43 12.112298628198626 38.928980634497265 36.1211392650751 12.837581472229004 44-45 12.146316785959359 38.78581911254251 36.37083080806816 12.697033293429968 46-47 11.854844466374205 38.676708715869204 36.457698034712195 13.010748783044399 48-49 11.706753226586118 38.61008212672913 36.32460971645114 13.358554930233607 50-51 11.452047245080726 38.578013251668985 36.54492165483035 13.425017848419946 52-53 11.0278304196921 38.57554946259321 36.96464425686805 13.431975860846649 54-55 10.999382563750606 38.31624007624649 37.03026940509454 13.654107954908367 56-57 10.846335235562034 38.41888353326145 36.873056602261386 13.861724628915129 58-59 11.073544383210677 38.12810003769195 36.468777666329814 14.329577912767553 60-61 11.396027093802712 37.64505816101252 36.53806418811885 14.420850557065915 62-63 11.240214452366253 37.47819346048023 36.634360218359696 14.647231868793826 64-65 11.419615484801731 36.993432784980065 36.81422445749094 14.772727272727273 66-67 11.603508692831577 36.89537793340797 36.57013252779677 14.930980845963676 68-69 11.648187024335009 36.57979648177004 36.647435479911216 15.124581013983738 70-71 11.801078063794419 36.291139858922215 36.65993137771943 15.247850699563939 72-73 11.998987511810274 35.818524022785994 36.60633506843067 15.57615339697306 74-75 12.246030850970515 35.27912484493064 36.535844517123415 15.938999786975428 76-77 12.589660305860061 34.84045352701463 37.01310255986125 15.556783607264052 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 105324.0 1 58348.0 2 9614.5 3 6850.0 4 5843.0 5 5323.5 6 4356.5 7 3828.5 8 3412.5 9 3077.0 10 2972.0 11 2740.5 12 2594.0 13 2574.0 14 2643.0 15 2700.5 16 2825.5 17 2962.0 18 3105.5 19 3418.0 20 3763.0 21 4243.0 22 4547.0 23 4803.0 24 5302.5 25 5931.0 26 6316.0 27 6562.5 28 7202.5 29 7880.5 30 8165.0 31 8506.0 32 8994.5 33 9466.5 34 9779.5 35 9768.0 36 9811.0 37 9874.5 38 9720.0 39 9545.0 40 9433.0 41 9147.5 42 8906.0 43 8838.0 44 8516.0 45 8186.5 46 8072.0 47 8163.5 48 8362.0 49 8016.5 50 7813.0 51 7002.0 52 6049.0 53 5743.0 54 4983.5 55 4345.5 56 3756.5 57 3352.0 58 3491.0 59 3369.5 60 2966.0 61 2823.0 62 2446.0 63 1563.5 64 785.0 65 512.0 66 460.5 67 370.5 68 319.0 69 286.5 70 267.0 71 238.0 72 217.5 73 234.0 74 242.0 75 234.0 76 206.5 77 159.5 78 132.0 79 139.0 80 149.0 81 128.0 82 81.5 83 59.0 84 49.5 85 31.0 86 21.5 87 21.0 88 19.0 89 13.5 90 10.5 91 11.0 92 10.0 93 7.0 94 5.0 95 4.0 96 3.0 97 3.0 98 3.0 99 5.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011769124827845248 2 0.010517090271691499 3 0.011268311005383749 4 0.013021159383998998 5 0.010767497182922248 6 0.007512207336922499 7 0.0125203455615375 8 0.01527482158507575 9 0.015024414673844999 10-11 0.017027669963690997 12-13 0.014022787028921999 14-15 0.008388631526230124 16-17 0.0071365969700763745 18-19 0.019406535620383122 20-21 0.025165894578690375 22-23 0.02241141855515212 24-25 0.018905721797921625 26-27 0.027544760235382496 28-29 0.008138224614999374 30-31 0.008013021159384 32-33 0.008764241893076249 34-35 0.010767497182922248 36-37 0.011518717916614497 38-39 0.011894328283460622 40-41 0.009390259171153124 42-43 0.014398397395768122 44-45 0.013021159383998998 46-47 0.02391386002253662 48-49 0.022286215099536745 50-51 0.038813071240766245 52-53 0.04119193689745837 54-55 0.029673218980843873 56-57 0.020408163265306124 58-59 0.015149618129460371 60-61 0.017653687241767874 62-63 0.025165894578690375 64-65 0.023538249655690496 66-67 0.028295980969074746 68-69 0.04306998873168899 70-71 0.05220984099161137 72-73 0.08338550143983973 74-75 0.08388631526230124 76-77 0.08613997746337798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 399350.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.07483102254464 #Duplication Level Percentage of deduplicated Percentage of total 1 86.00058895871757 42.20464370988384 2 7.96832461904289 7.820883684246245 3 2.712705572062826 3.9937670268869554 4 1.2205775135363586 2.39598540906858 5 0.6347778993356316 1.5575809073370988 6 0.38898880869385694 1.1453736033787185 7 0.24264387237971818 0.8335394924983391 8 0.1462524229388332 0.5741850353888778 9 0.119183863218948 0.5264035153275554 >10 0.5037826493199007 4.3261145294063805 >50 0.034654260747854486 1.1779515992413638 >100 0.02497145259771864 2.0490355525657074 >500 0.0010192429631721892 0.34263135689186125 >1k 5.096214815860946E-4 0.3268753163924545 >5k 0.0 0.0 >10k+ 0.0010192429631721892 30.725029261486007 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66466 16.643545761863027 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56387 14.119694503568297 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1307 0.3272818329785902 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 748 0.18730436960060098 No Hit TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 622 0.15575309878552648 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 443 0.11093026167522224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.001001627644923 0.0 2 0.0 0.0 0.0 0.00626017278076875 0.0 3 0.0 0.0 0.0 0.007261800425691749 0.0 4 0.0 0.0 0.0 0.011017904094152998 0.0 5 0.0 0.0 0.0 0.0195317390759985 0.0 6 0.0 0.0 0.0 0.032552898459997495 0.0 7 0.0 0.0 0.0 0.05108300989107299 0.0 8 0.0 0.0 0.0 0.06685864529861024 0.0 9 0.0 0.0 0.0 0.08588957055214724 0.0 10 0.0 0.0 0.0 0.10366846124953048 0.0 11 0.0 0.0 0.0 0.12294979341429824 0.0 12 0.0 0.0 0.0 0.147239263803681 0.0 13 0.0 0.0 0.0 0.17052710654814074 0.0 14 0.0 0.0 0.0 0.20433203956429197 0.0 15 0.0 0.0 0.0 0.24715162138475022 0.0 16 0.0 0.0 0.0 0.28997120320520847 0.0 17 0.0 0.0 0.0 0.3327907850256667 2.5040691123075E-4 18 0.0 0.0 0.0 0.36809815950920244 2.5040691123075E-4 19 0.0 0.0 0.0 0.4039063478151997 2.5040691123075E-4 20 0.0 0.0 0.0 0.44322023287842743 2.5040691123075E-4 21 0.0 0.0 0.0 0.4750219106047327 2.5040691123075E-4 22 0.0 0.0 0.0 0.5155878302241141 2.5040691123075E-4 23 0.0 0.0 0.0 0.5644171779141104 2.5040691123075E-4 24 0.0 0.0 0.0 0.6162514085388757 2.5040691123075E-4 25 0.0 0.0 0.0 0.6625766871165644 2.5040691123075E-4 26 0.0 0.0 0.0 0.7169149868536372 2.5040691123075E-4 27 0.0 0.0 0.0 0.7627394516088644 2.5040691123075E-4 28 0.0 0.0 0.0 0.8108175785651685 2.5040691123075E-4 29 0.0 0.0 0.0 0.8621509953674722 2.5040691123075E-4 30 0.0 0.0 0.0 0.9064730186553149 2.5040691123075E-4 31 0.0 0.0 0.0 0.9585576561913108 2.5040691123075E-4 32 0.0 0.0 0.0 1.0053837485914612 2.5040691123075E-4 33 0.0 0.0 0.0 1.0657318141980718 2.5040691123075E-4 34 0.0 0.0 0.0 1.1290847627394516 2.5040691123075E-4 35 0.0 0.0 0.0 1.19193689745837 2.5040691123075E-4 36 0.0 0.0 0.0 1.2673093777388256 2.5040691123075E-4 37 0.0 0.0 0.0 1.3314135470138977 2.5040691123075E-4 38 0.0 0.0 0.0 1.403029923625892 2.5040691123075E-4 39 0.0 0.0 0.0 1.470138975835733 2.5040691123075E-4 40 0.0 0.0 0.0 1.5357455865781895 2.5040691123075E-4 41 0.0 0.0 0.0 1.6058595217227996 2.5040691123075E-4 42 0.0 0.0 0.0 1.683736071115563 2.5040691123075E-4 43 0.0 0.0 0.0 1.7621134343307876 2.5040691123075E-4 44 0.0 0.0 0.0 1.853762363841242 2.5040691123075E-4 45 0.0 0.0 0.0 1.9396519343933893 2.5040691123075E-4 46 0.0 0.0 0.0 2.043069988731689 2.5040691123075E-4 47 0.0 0.0 0.0 2.138975835733066 2.5040691123075E-4 48 0.0 0.0 0.0 2.244397145361212 2.5040691123075E-4 49 0.0 0.0 0.0 2.3540753724802803 2.5040691123075E-4 50 0.0 0.0 0.0 2.446725929635658 2.5040691123075E-4 51 0.0 0.0 0.0 2.5486415425065734 2.5040691123075E-4 52 0.0 0.0 0.0 2.6505571553774883 2.5040691123075E-4 53 0.0 0.0 0.0 2.761737823963941 2.5040691123075E-4 54 0.0 0.0 0.0 2.8734193063728557 2.5040691123075E-4 55 0.0 0.0 0.0 2.9868536371603858 2.5040691123075E-4 56 0.0 0.0 0.0 3.1133091273319144 2.5040691123075E-4 57 0.0 0.0 0.0 3.2602979842243647 2.5040691123075E-4 58 0.0 0.0 0.0 3.3895079504194316 2.5040691123075E-4 59 0.0 0.0 0.0 3.524477275572806 2.5040691123075E-4 60 0.0 0.0 0.0 3.6667084011518716 2.5040691123075E-4 61 0.0 0.0 0.0 3.8081883059972457 2.5040691123075E-4 62 0.0 0.0 0.0 3.9476649555527734 2.5040691123075E-4 63 0.0 0.0 0.0 4.092149743332916 2.5040691123075E-4 64 0.0 0.0 0.0 4.240641041692751 2.5040691123075E-4 65 0.0 0.0 0.0 4.405158382371353 2.5040691123075E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACCGA 25 2.2289617E-4 56.792084 16 TCTAGCG 25 2.2303418E-4 56.784973 4 CGAATGC 20 0.006573286 53.242584 19 CAATACG 20 0.006573286 53.242584 14 AATACGA 20 0.006573286 53.242584 15 CTAGCGG 20 0.006576543 53.235912 5 TACCGAG 30 5.481801E-4 47.326736 17 TCTACCG 40 0.0022565825 35.495056 15 CGGCCGT 40 0.0022579697 35.49061 29 ATAGTTG 45 0.004011758 31.563015 60 GGACATC 50 0.0067191017 28.403158 53 GCATCTC 50 0.0067314436 28.392487 3 CTCGAAC 65 7.318486E-4 27.307306 23 GAGTACT 95 8.417459E-6 26.150974 6 CGAACTC 75 0.0016860387 23.666332 25 GGGTAAT 100 3.5750703E-4 21.294365 3 GGCCTCG 85 0.003488012 20.882057 25 GGAACTC 120 5.1209405E-5 20.705448 1 TAAGAGG 90 0.004856281 19.721943 23 ATCTAGT 90 0.004859801 19.719475 15 >>END_MODULE