FastQCFastQC Report
Thu 26 May 2016
SRR936173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399350
Sequences flagged as poor quality0
Sequence length125
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214865.380242894703894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147663.6975084512332543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136283.4125453862526607No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133153.3341680230374355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120513.017653687241768No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59391.487166645799424No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52661.3186427945411294No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25570.6402904720170277No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23130.5791911856767247No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19600.49079754601227No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16170.4049079754601227No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.22837110304244398No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8510.2130962814573682No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.18605233504444724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7330.18354826593213971No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6660.16677100287967947No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.16451734067860274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5290.13246525604106674No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5110.12795793163891325No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT4770.11944409665706773No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4030.10091398522599224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAC150.00422974559.5224998-99
AACGTAC351.3369572E-859.52248498-99
CTTACGC351.3369572E-859.52248498-99
TACCGGT201.7683468E-459.52248498-99
CTACCGT150.004231843659.51503496-97
GCGCGCG201.7694425E-459.5150396-97
CGCGCGG201.7694425E-459.5150396-97
CGGTTAC150.00423814459.49267628-29
TTACGCA6250.058.57012100-101
ACGTATC2150.058.138245100-101
CCGTACA2650.057.276352100-101
CGAGTAC4850.057.067947100-101
AGTATCA19500.056.927914100-101
TACGCAG8150.056.601135100-101
CCCGGTA1900.056.3897298-99
CGTATCA7950.056.15329100-101
CTCCGTA850.056.02116498-99
ACCGGTA800.055.8023398-99
TCCGTAT800.055.8023398-99
TCCGTAC750.055.5543298-99
Produced by FastQC (version 0.11.5)