FastQCFastQC Report
Thu 26 May 2016
SRR936172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399576
Sequences flagged as poor quality0
Sequence length77
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6679316.715968927062686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5683814.2245780527359No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12820.3208400904959257No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7090.1774380843694316No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6690.1674274731215088No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG4230.10586221394678359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG150.002100598571.0135346
CTCGAGT150.002102686670.9957456
GATCCGA252.225331E-456.8108247
ATACGAA252.2267087E-456.8037116
AGGGCCG252.2267087E-456.8037119
CAATACG252.2267087E-456.8037114
CCGATCC200.00657122453.24680745
GGCCGTG200.00657122453.24680721
GGGCCGT305.476275E-447.33642220
AATACGA305.476275E-447.33642215
CGCCAGG305.476275E-447.33642215
GTGGGCC305.4796576E-447.33049825
CGCAATA305.483041E-447.32457412
GCGCAAT305.4864265E-447.3186511
TCAGCGC305.4932025E-447.3068056
CTCAGCG305.4932025E-447.3068055
TACGAAT350.001169867140.57407817
GCGCTCC350.001172030640.5588389
AGCGCTC350.001172752540.5537648
CAGCGCT350.001173474740.548697