##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936172_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 399576 Sequences flagged as poor quality 0 Sequence length 77 %GC 24 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.73946132900875 40.0 35.0 41.0 31.0 41.0 2 36.51768624742227 39.0 35.0 41.0 31.0 41.0 3 36.29722756121489 39.0 35.0 41.0 31.0 41.0 4 36.12824594069714 39.0 35.0 41.0 31.0 41.0 5 35.91719973171562 38.0 35.0 40.0 31.0 41.0 6 35.55555638977316 38.0 35.0 40.0 30.0 41.0 7 35.04975023524936 37.0 35.0 40.0 30.0 41.0 8 34.60685826976595 35.0 35.0 40.0 30.0 41.0 9 34.361097763629445 35.0 35.0 40.0 29.0 41.0 10-11 34.1017303341542 35.0 34.0 40.0 26.5 41.0 12-13 33.65645083788816 35.0 33.5 40.0 23.5 41.0 14-15 33.29612889663043 35.0 33.0 40.0 21.5 41.0 16-17 32.87507007427874 35.0 33.0 39.5 20.0 41.0 18-19 32.38241160630268 35.0 33.0 39.0 16.0 41.0 20-21 31.66922437784051 35.0 33.0 39.0 3.5 41.0 22-23 30.74169619796985 35.0 31.5 39.0 2.0 41.0 24-25 30.079145143852486 35.0 30.5 39.0 2.0 41.0 26-27 29.070302020141348 34.0 27.5 37.5 2.0 40.0 28-29 29.138083868901035 35.0 27.5 38.0 2.0 40.0 30-31 29.4626291368851 35.0 29.5 38.0 2.0 40.5 32-33 29.332092017538592 35.0 29.0 38.0 2.0 41.0 34-35 29.107019690872324 35.0 28.0 38.0 2.0 41.0 36-37 28.84877344985685 35.0 27.0 37.0 2.0 40.0 38-39 28.565806004364624 35.0 27.0 37.0 2.0 40.0 40-41 28.264748133021 35.0 26.0 36.0 2.0 40.0 42-43 27.938845175886442 35.0 26.0 36.0 2.0 40.0 44-45 27.63470528760486 35.0 25.5 35.0 2.0 40.0 46-47 27.300196958776304 35.0 24.0 35.0 2.0 39.0 48-49 26.94360397020842 35.0 23.0 35.0 2.0 39.0 50-51 26.560047400244258 34.5 20.0 35.0 2.0 38.5 52-53 26.10990524956454 34.0 14.5 35.0 2.0 37.5 54-55 25.66373480889743 34.0 4.5 35.0 2.0 37.0 56-57 25.22347938815144 33.0 2.0 35.0 2.0 36.5 58-59 24.750688229523295 33.0 2.0 35.0 2.0 36.0 60-61 24.316925941497985 33.0 2.0 35.0 2.0 35.5 62-63 23.863162452199333 33.0 2.0 35.0 2.0 35.0 64-65 23.396132400344364 33.0 2.0 35.0 2.0 35.0 66-67 22.92932258193685 33.0 2.0 35.0 2.0 35.0 68-69 22.445712455202514 32.0 2.0 35.0 2.0 35.0 70-71 21.920355576911525 31.5 2.0 35.0 2.0 35.0 72-73 21.402183814843735 31.0 2.0 35.0 2.0 35.0 74-75 20.881912327066694 31.0 2.0 35.0 2.0 35.0 76-77 20.07937038260556 28.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9014585300391431 1101 2 0.8449193579967726 1101 3 0.9082176840354563 1101 4 0.7072463223721925 1101 5 0.7334699913154168 1101 6 0.9698501717656143 1101 7 0.8291428046721521 1101 8 0.8975114149222989 1101 9 0.7628927434639508 1101 10-11 0.8844985961170124 1101 12-13 0.9824793909345644 1101 14-15 0.8933272548252233 1101 16-17 1.0209766433688117 1101 18-19 0.8691661902316881 1101 20-21 0.7269489443030892 1101 22-23 0.7745635409018874 1101 24-25 0.6340397337853538 1101 26-27 0.34841363843091955 1101 28-29 0.32953832507048375 1101 30-31 0.4124762581860715 1101 32-33 0.6083730791352835 1101 34-35 0.6345131523938683 1101 36-37 0.6840454314222946 1101 38-39 0.5585523708976865 1101 40-41 0.4977443435953184 1101 42-43 0.1180626537763203 1101 44-45 0.17824339098367759 1101 46-47 0.3869707471800581 1101 48-49 0.2954382836891156 1101 50-51 0.15300199001363524 1101 52-53 0.44217982566349434 1101 54-55 0.4316785823472884 1101 56-57 0.2009657502575557 1101 58-59 0.23526784930248112 1101 60-61 0.1376078349633083 1101 62-63 -0.08416833117210842 1101 64-65 -0.1424527731532521 1101 66-67 -0.8024629882152965 1101 68-69 -0.6070405561973189 1101 70-71 -0.5940405209409612 1101 72-73 -0.8936833627916734 1101 74-75 -0.8525736423650194 1101 76-77 -1.1011156467252228 1102 1 0.17480994553329765 1102 2 0.3111678746945188 1102 3 0.21304901137906995 1102 4 0.0023217588868575945 1102 5 -0.22684531636385685 1102 6 0.029139881885974717 1102 7 -0.06681413718239781 1102 8 0.090196615803805 1102 9 0.10456082279943502 1102 10-11 0.3761963414975824 1102 12-13 0.4997619812040952 1102 14-15 0.49264069778165265 1102 16-17 0.3519734224839155 1102 18-19 0.5997391687026123 1102 20-21 0.61785418247905 1102 22-23 0.4132056836379441 1102 24-25 1.1060900813018826 1102 26-27 1.0480618838962528 1102 28-29 1.2575812136956728 1102 30-31 0.9348147901406421 1102 32-33 0.9269355473502401 1102 34-35 0.5667982854672715 1102 36-37 0.5322908967554021 1102 38-39 1.0375233829336246 1102 40-41 0.9753973356278678 1102 42-43 1.263523748877187 1102 44-45 0.9599606311938835 1102 46-47 1.1514816812366817 1102 48-49 1.1032531675677397 1102 50-51 1.3395124155094855 1102 52-53 0.837009457804534 1102 54-55 0.9404470176753108 1102 56-57 1.4236390847210245 1102 58-59 1.3313646453696109 1102 60-61 1.521545790549002 1102 62-63 1.6403273460613228 1102 64-65 1.1313605850430548 1102 66-67 1.2661121940189801 1102 68-69 1.0208116221379946 1102 70-71 0.8186099282877244 1102 72-73 0.5027058557199382 1102 74-75 0.6674382240530186 1102 76-77 0.8013339209981218 1103 1 0.7123828997870447 1103 2 0.5653675372684788 1103 3 0.9463129221306943 1103 4 0.8107477229324189 1103 5 0.7931338568616297 1103 6 0.8108865863314421 1103 7 1.0231203957085668 1103 8 0.5556346642220191 1103 9 0.7278787378617153 1103 10-11 0.9757450947164514 1103 12-13 1.2677735085816195 1103 14-15 1.1203580671501499 1103 16-17 1.2020690803435983 1103 18-19 0.98947431348099 1103 20-21 1.2923551067680705 1103 22-23 0.7536951935629546 1103 24-25 0.9446139634772308 1103 26-27 1.3110887084589322 1103 28-29 1.1602806219892514 1103 30-31 1.0772521685502205 1103 32-33 1.3439473088271612 1103 34-35 1.2245691748028307 1103 36-37 1.3518325462682306 1103 38-39 1.5469277210377435 1103 40-41 1.6812877609622667 1103 42-43 1.8315780599387885 1103 44-45 1.5691397495270927 1103 46-47 1.4109203270119934 1103 48-49 1.5500601324286123 1103 50-51 1.695508992814755 1103 52-53 1.6348969124982276 1103 54-55 1.6884712994341236 1103 56-57 1.7686128090810875 1103 58-59 1.5171305944005198 1103 60-61 1.0144985912658306 1103 62-63 1.1970641618250895 1103 64-65 0.8762447058383458 1103 66-67 0.7355622218687401 1103 68-69 0.9506625250351775 1103 70-71 0.9624720841010586 1103 72-73 1.3763446484128075 1103 74-75 1.2794991867666319 1103 76-77 1.227035613778984 1104 1 -0.1147344640124075 1104 2 -0.13918507029139704 1104 3 -0.10324959950717982 1104 4 -0.2537252572709008 1104 5 0.0847257745275769 1104 6 -0.14283984806254324 1104 7 0.15564643983407933 1104 8 -0.1992830331940283 1104 9 -0.1227648903760965 1104 10-11 -0.22749743825179536 1104 12-13 -0.3853163790588283 1104 14-15 -0.02425371014503952 1104 16-17 -0.06000485035227854 1104 18-19 -0.11171616270949158 1104 20-21 0.034054033529788086 1104 22-23 0.17092837897195068 1104 24-25 0.5037522255236233 1104 26-27 0.13109704887374818 1104 28-29 0.15557930325951475 1104 30-31 0.5001662780142304 1104 32-33 0.5721206006157864 1104 34-35 0.6764662257580483 1104 36-37 0.5014446382960536 1104 38-39 0.3774717363966964 1104 40-41 0.4762473178187179 1104 42-43 0.2916581593942986 1104 44-45 0.3337787511951795 1104 46-47 0.11934021181984633 1104 48-49 -0.014558411618221356 1104 50-51 0.08398348373471975 1104 52-53 0.3926590060997128 1104 54-55 0.27103747197055483 1104 56-57 0.33611115673475567 1104 58-59 0.1500067785820569 1104 60-61 0.06602819187011377 1104 62-63 0.042797960962722925 1104 64-65 0.4922630232776797 1104 66-67 0.1546916714013591 1104 68-69 0.1007585184887354 1104 70-71 -0.029218974344789927 1104 72-73 -0.19715329008843696 1104 74-75 -0.28170450819449755 1104 76-77 -0.808419017511735 1105 1 0.11367822454678134 1105 2 0.25349914350213254 1105 3 0.27289106720087375 1105 4 0.15539386868353233 1105 5 -0.021876125031674576 1105 6 0.20213811456704178 1105 7 0.028931559953271346 1105 8 0.26766091118489044 1105 9 0.1398833186995745 1105 10-11 -0.0096921741137308 1105 12-13 0.015011077642391513 1105 14-15 -0.10917518261384629 1105 16-17 -0.18331324938387183 1105 18-19 0.2273722350805656 1105 20-21 0.1536145276634997 1105 22-23 0.09299058022590856 1105 24-25 0.029514221923150785 1105 26-27 -0.19337219192332356 1105 28-29 -0.1757184185109253 1105 30-31 0.014304337484091434 1105 32-33 -0.3774572751058578 1105 34-35 -0.3786815177393805 1105 36-37 -0.2705694532055887 1105 38-39 -0.4085097025504787 1105 40-41 -0.51906586114956 1105 42-43 -0.4348378987098691 1105 44-45 -0.5194166675148608 1105 46-47 -0.7119730292255326 1105 48-49 -0.5520917780593422 1105 50-51 -0.5143932001066105 1105 52-53 -0.5883531610118382 1105 54-55 -0.4523236925958365 1105 56-57 -0.2055503368402647 1105 58-59 -0.26469152671311846 1105 60-61 -0.08694530120828858 1105 62-63 -0.13831197758095115 1105 64-65 -0.11412160643241975 1105 66-67 -0.004908552076202 1105 68-69 0.0054391318104833886 1105 70-71 -0.04799729702155631 1105 72-73 -0.13104117884627442 1105 74-75 -0.3311566777891315 1105 76-77 -0.3817786301506345 1106 1 0.5104267074740534 1106 2 0.4735275225740736 1106 3 0.5359258164687546 1106 4 0.5254237572805778 1106 5 0.5191980530319071 1106 6 0.4194644437038946 1106 7 0.18264650123274606 1106 8 0.10141691154717591 1106 9 0.2209448167522865 1106 10-11 0.2221633341433673 1106 12-13 0.5778827981991057 1106 14-15 0.46553876237905456 1106 16-17 0.6178747468725092 1106 18-19 0.49962263510472127 1106 20-21 0.29398526702889427 1106 22-23 0.17359922074759737 1106 24-25 0.38592819408750856 1106 26-27 0.13675340056528285 1106 28-29 0.07438724824631038 1106 30-31 0.19332646712210888 1106 32-33 0.11562155229503901 1106 34-35 0.17708788720582902 1106 36-37 -0.04805035771303068 1106 38-39 0.16287573958942758 1106 40-41 0.41065819766153666 1106 42-43 0.14005718929544742 1106 44-45 0.132245319623987 1106 46-47 -0.02153134218027475 1106 48-49 0.024207168292136316 1106 50-51 -0.1287321790638316 1106 52-53 -0.06829912001313687 1106 54-55 0.10912152673816067 1106 56-57 0.045211973336950706 1106 58-59 -0.2581024496367661 1106 60-61 -0.808181300362957 1106 62-63 -0.7448240688770262 1106 64-65 -0.356604171417473 1106 66-67 -0.2573086969906093 1106 68-69 -0.6706696143779638 1106 70-71 -0.7045275026188769 1106 72-73 -0.9078427970571852 1106 74-75 -1.0931875928632486 1106 76-77 -1.1319899636651094 1107 1 -0.5224431825446203 1107 2 -0.47466366508587754 1107 3 -0.7512015266868062 1107 4 -0.7229472740537233 1107 5 -0.5047280727203258 1107 6 -0.4979458073855412 1107 7 -0.29792346487364085 1107 8 -0.32657644807100183 1107 9 -0.2885592297303887 1107 10-11 -0.34586253567747605 1107 12-13 -0.23222649141838048 1107 14-15 -0.31557073475035935 1107 16-17 -0.05910526281138573 1107 18-19 -0.14620983359557016 1107 20-21 -0.10689320014963499 1107 22-23 -0.28258463482409724 1107 24-25 -0.24278454022804752 1107 26-27 -0.9296019982034807 1107 28-29 -0.8293410494641407 1107 30-31 -0.5713100411437857 1107 32-33 -0.38068127678429775 1107 34-35 -0.6166037500633159 1107 36-37 -0.7442494308264038 1107 38-39 -0.4748430125721548 1107 40-41 -0.4838081508201668 1107 42-43 -0.5227601310338024 1107 44-45 -0.6853828189939826 1107 46-47 -0.7030143607871793 1107 48-49 -0.43307995905102104 1107 50-51 -0.47735914178085537 1107 52-53 -0.37397802988378714 1107 54-55 -0.33673169315531837 1107 56-57 -0.4051027530931499 1107 58-59 -0.4867909742269312 1107 60-61 -0.7692797301893357 1107 62-63 -0.6904974450961596 1107 64-65 -0.6566560494004605 1107 66-67 -0.3947749763016688 1107 68-69 -0.33034658747058643 1107 70-71 -0.3689288306952392 1107 72-73 -0.11155380210664134 1107 74-75 -0.4180613564379918 1107 76-77 -0.472634634066992 1108 1 0.14888046177480163 1108 2 -0.2805708380816583 1108 3 -0.02424418960288932 1108 4 0.08905460123929032 1108 5 0.1790037603785919 1108 6 0.2436700700953196 1108 7 0.33273757591398123 1108 8 -0.18255394455482588 1108 9 0.05083755101660614 1108 10-11 0.2745312758555727 1108 12-13 0.5887611629026068 1108 14-15 0.3910396731258743 1108 16-17 0.3510056920253106 1108 18-19 0.6762468002534732 1108 20-21 0.4660556981157242 1108 22-23 0.1989281015409574 1108 24-25 0.31238032928281356 1108 26-27 0.34120594053694475 1108 28-29 0.5586414478010582 1108 30-31 0.5371131499798274 1108 32-33 0.2549961728172008 1108 34-35 0.4195894051171791 1108 36-37 0.4195574139931004 1108 38-39 0.46091257423828225 1108 40-41 0.2337102114239329 1108 42-43 0.48503796241829633 1108 44-45 0.661358859393264 1108 46-47 0.7159104712178248 1108 48-49 0.6592000847058159 1108 50-51 0.747972941357137 1108 52-53 0.855542207653329 1108 54-55 0.8097286912797479 1108 56-57 0.4898183283258213 1108 58-59 0.8243201368841824 1108 60-61 1.0650225045348414 1108 62-63 1.3133625330254226 1108 64-65 0.9490323394102944 1108 66-67 1.2131325092429464 1108 68-69 1.1325382382834377 1108 70-71 0.987204940206464 1108 72-73 0.6095119894232859 1108 74-75 0.3581380497192228 1108 76-77 0.35135348907725117 1109 1 -0.0055527593250843665 1109 2 -0.13410619161701476 1109 3 -0.4069041222628229 1109 4 -0.33788142569673596 1109 5 -0.24086155948672427 1109 6 -0.1928057997138879 1109 7 -0.2470604038839994 1109 8 -0.24922061122149586 1109 9 -0.05410072058833748 1109 10-11 -0.06132731000301561 1109 12-13 0.10236946817757797 1109 14-15 0.40644371352991016 1109 16-17 0.5887722464585252 1109 18-19 0.32136464537131104 1109 20-21 0.4546078866679082 1109 22-23 0.0974690667485909 1109 24-25 -0.14522348665433427 1109 26-27 -0.3101128024484616 1109 28-29 -0.355443849617572 1109 30-31 -0.555928376395034 1109 32-33 -0.2519061840851542 1109 34-35 -0.3325991470713703 1109 36-37 -0.4353247408890546 1109 38-39 -0.7138629588705818 1109 40-41 -0.6525973082169223 1109 42-43 -0.6551696694560043 1109 44-45 -0.35446605643165086 1109 46-47 -0.37367987670585734 1109 48-49 -0.3295765707375047 1109 50-51 -0.37161179489426743 1109 52-53 -0.23825689447910747 1109 54-55 -0.3540760225249642 1109 56-57 -0.41610198592782766 1109 58-59 -0.6643162267521845 1109 60-61 -0.571200840021838 1109 62-63 -0.35765317965695687 1109 64-65 -0.44521569650440895 1109 66-67 -0.5502793875022824 1109 68-69 -0.5403293262507916 1109 70-71 -0.5624303010954428 1109 72-73 -0.8258134875688583 1109 74-75 -0.6996621747476688 1109 76-77 -0.4112166568262303 1110 1 -0.5105099525039876 1110 2 -0.17279562638034918 1110 3 -0.39326309303287843 1110 4 -0.2507281376196602 1110 5 -0.611741257156261 1110 6 -0.3183919149073944 1110 7 -0.2508395346839123 1110 8 -0.4881223304927289 1110 9 -0.18663857021625319 1110 10-11 -0.8107907667412988 1110 12-13 -0.5925036276770399 1110 14-15 -0.8678261210747991 1110 16-17 -0.843722446428778 1110 18-19 -0.5051919132868576 1110 20-21 -1.0573127049293554 1110 22-23 -0.7974850937944851 1110 24-25 -0.325697394322237 1110 26-27 -0.19040241916994916 1110 28-29 -0.02517231207120929 1110 30-31 0.27387076980134495 1110 32-33 0.2135625914298096 1110 34-35 0.5201197275874563 1110 36-37 0.4675259367884159 1110 38-39 0.23699959274254567 1110 40-41 0.5856162360012398 1110 42-43 0.8640788200580758 1110 44-45 0.6255535390613822 1110 46-47 0.45247135851004217 1110 48-49 0.05426181310087941 1110 50-51 0.22191586041168065 1110 52-53 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74-75 -0.17268788640974364 2313 76-77 -0.3597933326756326 2314 1 -0.9061247442344182 2314 2 -0.9894061794471227 2314 3 -0.7246904189647836 2314 4 -0.8453050596263907 2314 5 -0.8529462619328712 2314 6 -1.240816022422905 2314 7 -1.0959103491194853 2314 8 -0.998885939200072 2314 9 -1.1277285653005151 2314 10-11 -1.3164995139703919 2314 12-13 -1.2994955677236035 2314 14-15 -1.017327127409601 2314 16-17 -1.3510172144152683 2314 18-19 -1.3752703795769605 2314 20-21 -1.3488483625714736 2314 22-23 -1.4931662015975213 2314 24-25 -1.8104145539538372 2314 26-27 -1.5225230494972664 2314 28-29 -1.4943631632356826 2314 30-31 -1.7422189934085388 2314 32-33 -1.597591957481768 2314 34-35 -1.8013833748917456 2314 36-37 -2.3950119746726735 2314 38-39 -1.7773441563879224 2314 40-41 -1.7439931947935285 2314 42-43 -1.779226502850186 2314 44-45 -2.0319030772421662 2314 46-47 -2.050614889484244 2314 48-49 -1.905577544328839 2314 50-51 -1.6365503369412444 2314 52-53 -1.859332104409738 2314 54-55 -1.6468531918081588 2314 56-57 -1.3993531724922654 2314 58-59 -1.5889998095682998 2314 60-61 -1.7497179387196589 2314 62-63 -1.682361102256447 2314 64-65 -1.6401317242517628 2314 66-67 -1.3741202053190058 2314 68-69 -1.7686517066816094 2314 70-71 -1.7766356071682203 2314 72-73 -1.6732486828956183 2314 74-75 -1.438009503456442 2314 76-77 -1.2987060081710062 2315 1 -0.6189761498569126 2315 2 -0.5315748541459158 2315 3 -0.4756944350290411 2315 4 -0.2228150997870344 2315 5 -0.22443220570797706 2315 6 -0.05808509872812806 2315 7 -0.7445047266295219 2315 8 -0.9788056179149365 2315 9 -0.6237125010434852 2315 10-11 -0.8576641726049345 2315 12-13 -0.8296160496513139 2315 14-15 -0.7251584527108079 2315 16-17 -0.6271216320859523 2315 18-19 -0.4857121466452341 2315 20-21 -0.6791696477120368 2315 22-23 -0.6397525469790466 2315 24-25 -0.8937478872871729 2315 26-27 -0.7203142141558985 2315 28-29 -0.9451663760870872 2315 30-31 -1.1197290335691825 2315 32-33 -0.7502023992488418 2315 34-35 -0.8335118889479673 2315 36-37 -1.0436063521827137 2315 38-39 -1.0855770881148352 2315 40-41 -1.1837522309381079 2315 42-43 -1.0362546152999847 2315 44-45 -1.0057986595714858 2315 46-47 -0.8437875802472945 2315 48-49 -1.089312484087877 2315 50-51 -0.9779157987886329 2315 52-53 -1.1625449558153633 2315 54-55 -1.2823953604828553 2315 56-57 -1.3330881122513034 2315 58-59 -1.1130961951104652 2315 60-61 -1.4545372158280934 2315 62-63 -1.3741281705295414 2315 64-65 -1.1632240937296743 2315 66-67 -1.2837587595358713 2315 68-69 -1.4323063251555013 2315 70-71 -1.0597681372887031 2315 72-73 -0.985497678879554 2315 74-75 -0.9360014713279305 2315 76-77 -1.0517180563637716 2316 1 -1.3382603663689778 2316 2 -0.897090012122959 2316 3 -1.0311107907172783 2316 4 -1.7766943335361916 2316 5 -1.2569516640724032 2316 6 -1.8394939198533322 2316 7 -1.6030539678421647 2316 8 -1.3042845246924841 2316 9 -1.9407772200966917 2316 10-11 -1.7694706907127653 2316 12-13 -2.4379787038077616 2316 14-15 -3.3469642600108074 2316 16-17 -2.6246115919253086 2316 18-19 -1.9977781096121454 2316 20-21 -1.7922858292523571 2316 22-23 -1.5667170421277845 2316 24-25 -1.6041825462771122 2316 26-27 -1.6284733995701224 2316 28-29 -1.8529968128785015 2316 30-31 -1.922648676743652 2316 32-33 -2.089284390932388 2316 34-35 -1.7381703457841624 2316 36-37 -1.8282138085568853 2316 38-39 -2.299308118430787 2316 40-41 -1.9028543029429414 2316 42-43 -1.7921143373741195 2316 44-45 -1.8646899770538035 2316 46-47 -1.7290365406908421 2316 48-49 -1.6160921692000052 2316 50-51 -1.5239761463736627 2316 52-53 -1.9270809760023049 2316 54-55 -2.1752188668978967 2316 56-57 -2.10814408836816 2316 58-59 -2.4173219476782606 2316 60-61 -2.1530480734177466 2316 62-63 -2.254743552411046 2316 64-65 -2.1430904618570494 2316 66-67 -1.749964289620813 2316 68-69 -1.4912545072644718 2316 70-71 -1.4468385428045742 2316 72-73 -1.5424600416047944 2316 74-75 -1.6786668230001034 2316 76-77 -1.5537705139818563 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10137.0 3 1904.0 4 3477.0 5 4040.0 6 3930.0 7 3842.0 8 4886.0 9 5715.0 10 8698.0 11 10573.0 12 6535.0 13 3995.0 14 3464.0 15 3229.0 16 3360.0 17 3347.0 18 3430.0 19 3642.0 20 3954.0 21 4141.0 22 4678.0 23 4893.0 24 5671.0 25 6232.0 26 6899.0 27 7996.0 28 8745.0 29 9832.0 30 11233.0 31 13183.0 32 15733.0 33 21497.0 34 48674.0 35 47976.0 36 23169.0 37 26964.0 38 28982.0 39 10717.0 40 203.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 13.4227650415457 31.757933727099807 43.94659125037541 10.872709980979076 2 13.37249464389404 31.997226838596 43.91080832148649 10.719470196023467 3 12.359803668820035 33.351771711189926 43.40360774812341 10.884816871866624 4 12.373748119519698 33.427868405177485 43.53452699506131 10.663856480241503 5 11.979695939688531 34.20278029464916 43.35637730710892 10.461146458553387 6 12.184878156224345 34.3322620692417 43.36341883422438 10.119440940309566 7 12.242149660204502 34.71220635536476 42.74214340246055 10.303500581970187 8 11.530413016270339 35.22778473091364 42.699123904881105 10.542678347934919 9 11.62705127306587 34.98428066521822 42.72805550827518 10.660612553440732 10-11 11.387923071724128 35.71562671312602 42.54022013121763 10.356230083932223 12-13 11.34020618556701 35.970332507706615 41.98430766881647 10.705153637909909 14-15 11.521605826627788 36.32694690210114 41.6147117345981 10.536735536672966 16-17 11.37236204549095 36.72513214800577 41.29374899887874 10.60875680762454 18-19 11.033711127575643 37.13630629367829 40.74821281963996 11.081769759106109 20-21 11.365997121581877 37.38689694011639 40.05368875539703 11.1934171829047 22-23 11.168419287253721 37.90892046748787 38.93145630630518 11.991203938953234 24-25 11.390123527872941 38.46674735802556 37.703600695431014 12.439528418670495 26-27 11.746801350172527 39.08328942477494 36.57205363143594 12.597855593616591 28-29 11.40830917052208 39.455482653458354 36.160997530950716 12.975210645068847 30-31 11.401093744133952 39.25114818105595 36.09005243464441 13.257705640165687 32-33 11.608960793711777 39.24771080779045 35.549462444983675 13.5938659535141 34-35 11.51341312629213 38.97077595398639 35.929482847517356 13.586328072204118 36-37 11.886520917543706 38.94142086623534 35.973795192030934 13.198263024190016 38-39 12.196785570359765 38.87865415470412 36.11515077181855 12.80940950311756 40-41 12.147629756724521 39.00792563196684 36.17870153794554 12.665743073363094 42-43 12.129874258309252 38.97041246828924 36.081119472753464 12.818593800648046 44-45 12.130398213695667 38.84047921819146 36.252640906769734 12.776481661343134 46-47 11.840786181744454 38.80082753338231 36.37554896677226 12.982837318100978 48-49 11.779834425275489 38.59355347387787 36.34396490766988 13.28264719317677 50-51 11.477859889449652 38.52389249379156 36.58450492670031 13.41374269005848 52-53 11.039842976437702 38.542499884210145 36.906545694432445 13.511111444919704 54-55 10.949924712461684 38.358969480697475 37.0440973443463 13.64700846249454 56-57 10.922006585493142 38.356680420860826 36.90053802803152 13.820774965614516 58-59 11.070713846777695 38.07841964233194 36.572178786471746 14.278687724418623 60-61 11.397799667071354 37.67250334806063 36.46419765448014 14.465499330387873 62-63 11.241077094653203 37.398786121885976 36.621243242499006 14.738893540961815 64-65 11.453120893149931 36.947194512935404 36.85582687710427 14.743857716810393 66-67 11.643499570701572 36.86572095852452 36.485860687027674 15.004918783746234 68-69 11.658878475425576 36.498681244124434 36.700969758117516 15.141470522332476 70-71 11.78573440933593 36.2775235245137 36.65091061623896 15.285831449911411 72-73 12.037801208025618 35.86330750551351 36.4894870712705 15.609404215190365 74-75 12.297487371728955 35.25908465231392 36.51408366910611 15.929344306851018 76-77 12.617901045156524 34.89958607042439 36.94719110270589 15.535321781713204 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 105706.0 1 58600.0 2 9664.0 3 6928.0 4 6022.0 5 5377.5 6 4297.0 7 3806.5 8 3443.0 9 3134.0 10 2943.0 11 2698.0 12 2625.0 13 2606.0 14 2602.5 15 2633.0 16 2792.5 17 2918.0 18 3049.5 19 3391.5 20 3763.0 21 4287.5 22 4651.0 23 4787.0 24 5248.0 25 5895.0 26 6217.0 27 6505.0 28 7182.5 29 7763.5 30 7955.0 31 8393.5 32 8960.0 33 9491.0 34 9776.5 35 9659.0 36 9825.0 37 10044.5 38 9741.5 39 9385.0 40 9265.0 41 9195.5 42 8984.0 43 8722.0 44 8489.0 45 8260.5 46 8093.0 47 8086.5 48 8252.0 49 8076.5 50 7888.0 51 7014.5 52 6154.0 53 5743.5 54 4935.0 55 4417.5 56 3846.0 57 3394.0 58 3560.5 59 3412.5 60 2928.0 61 2758.0 62 2423.5 63 1545.0 64 752.0 65 503.0 66 447.0 67 353.5 68 305.5 69 273.5 70 252.0 71 225.5 72 228.5 73 249.0 74 240.0 75 219.5 76 185.0 77 152.5 78 134.0 79 124.0 80 130.5 81 129.5 82 89.5 83 67.0 84 49.5 85 30.5 86 26.0 87 23.0 88 18.0 89 14.0 90 9.5 91 4.0 92 5.5 93 5.5 94 3.5 95 2.0 96 1.0 97 1.5 98 2.5 99 6.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00400424449916912 2 0.00800848899833824 3 0.011762468216309288 4 0.019270426652251385 5 0.012513264059903497 6 0.012012733497507358 7 0.017768834965062968 8 0.019020161371053317 9 0.017018039121468757 10-11 0.021773079464232084 12-13 0.020396620417642702 14-15 0.007758223717140169 16-17 0.006006366748753679 18-19 0.01601697799667648 20-21 0.012763529341101566 22-23 0.019770957214647524 24-25 0.0279045788535848 26-27 0.017893967605662003 28-29 0.007382825795343064 30-31 0.007758223717140169 32-33 0.008884417482531484 34-35 0.010511141810318937 36-37 0.007758223717140169 38-39 0.016267243277874547 40-41 0.012388131419304463 42-43 0.01664264119967165 44-45 0.023024405870222437 46-47 0.01889502873045428 48-49 0.014640518950087092 50-51 0.030031833743768395 52-53 0.036413598414319175 54-55 0.02690351772879252 56-57 0.015391314793681301 58-59 0.017893967605662003 60-61 0.022774140589024366 62-63 0.028154844134782867 64-65 0.022774140589024366 66-67 0.021773079464232084 68-69 0.036663863695517246 70-71 0.06469357518970109 72-73 0.08246241015476405 74-75 0.09322381724628105 76-77 0.08846877690351773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 399576.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.998552588564024 #Duplication Level Percentage of deduplicated Percentage of total 1 85.97691871395426 42.12744573008383 2 8.052064126842001 7.8907897513111545 3 2.6778608111914295 3.93633911346054 4 1.2198007705557208 2.3907388881458163 5 0.632674288506675 1.550006219841332 6 0.3632446623592343 1.0679077614674477 7 0.25013859622757084 0.857950041318035 8 0.15823115776624128 0.6202478163966836 9 0.10754144142121969 0.474243747563485 >10 0.49672699717472313 4.237007417993585 >50 0.03775656784305225 1.2820119018256964 >100 0.024490746709006826 1.992268495641373 >500 0.0010204477795419512 0.34450319826751313 >1k 5.102238897709756E-4 0.3205029754564237 >5k 0.0 0.0 >10k+ 0.0010204477795419512 30.90803694122708 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66793 16.715968927062686 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56838 14.2245780527359 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1282 0.3208400904959257 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 709 0.1774380843694316 No Hit TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 669 0.1674274731215088 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 423 0.10586221394678359 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0010010611247922797 0.0 2 0.0 0.0 0.0 0.005005305623961399 0.0 3 0.0 0.0 0.0 0.007758223717140168 0.0 4 0.0 0.0 0.0 0.012763529341101568 0.0 5 0.0 0.0 0.0 0.021522814183034016 0.0 6 0.0 0.0 0.0 0.032534486555749094 0.0 7 0.0 0.0 0.0 0.05180491320800048 0.0 8 0.0 0.0 0.0 0.07107533986025187 0.0 9 0.0 0.0 0.0 0.08383886920135343 0.0 10 0.0 0.0 0.0 0.1008569083228222 0.0 11 0.0 0.0 0.0 0.12187919194346007 0.0 12 0.0 0.0 0.0 0.14365227140769216 0.0 13 0.0 0.0 0.0 0.17843914549422388 0.0 14 0.0 0.0 0.0 0.21898212104831122 0.0 15 0.0 0.0 0.0 0.2670330550383406 0.0 16 0.0 0.0 0.0 0.3180871724027469 0.0 17 0.0 0.0 0.0 0.3598814743628246 0.0 18 0.0 0.0 0.0 0.4009249804793081 0.0 19 0.0 0.0 0.0 0.43871503794021666 0.0 20 0.0 0.0 0.0 0.48075960518149236 0.0 21 0.0 0.0 0.0 0.5230544377039662 0.0 22 0.0 0.0 0.0 0.5676016577572227 0.0 23 0.0 0.0 0.0 0.6103970208420926 0.0 24 0.0 0.0 0.0 0.6591987506757162 0.0 25 0.0 0.0 0.0 0.7102528680401226 0.0 26 0.0 0.0 0.0 0.761807515966925 0.0 27 0.0 0.0 0.0 0.8128616333313312 0.0 28 0.0 0.0 0.0 0.85840991450938 0.0 29 0.0 0.0 0.0 0.90896350131139 0.0 30 0.0 0.0 0.0 0.9562636394578253 0.0 31 0.0 0.0 0.0 1.0095701443530143 0.0 32 0.0 0.0 0.0 1.066130097903778 0.0 33 0.0 0.0 0.0 1.123691112579334 0.0 34 0.0 0.0 0.0 1.1772478827557211 0.0 35 0.0 0.0 0.0 1.238062346086852 0.0 36 0.0 0.0 0.0 1.302630788635954 0.0 37 0.0 0.0 0.0 1.369952149278235 0.0 38 0.0 0.0 0.0 1.4327687348589504 0.0 39 0.0 0.0 0.0 1.5010911566260237 0.0 40 0.0 0.0 0.0 1.5686627825495025 0.0 41 0.0 0.0 0.0 1.6399883876909525 0.0 42 0.0 0.0 0.0 1.710062666426412 0.0 43 0.0 0.0 0.0 1.7876449035978137 0.0 44 0.0 0.0 0.0 1.8752377520171382 0.0 45 0.0 0.0 0.0 1.9655835185296415 0.0 46 0.0 0.0 0.0 2.061935651790898 0.0 47 0.0 0.0 0.0 2.161290968426532 0.0 48 0.0 0.0 0.0 2.2638997337177407 0.0 49 0.0 0.0 0.0 2.368510621258534 0.0 50 0.0 0.0 0.0 2.46110877530182 0.0 51 0.0 0.0 0.0 2.5667207239674052 0.0 52 0.0 0.0 0.0 2.667577632290228 0.0 53 0.0 0.0 0.0 2.765681622519871 0.0 54 0.0 0.0 0.0 2.879302060183795 0.0 55 0.0 0.0 0.0 2.9954251506596994 0.0 56 0.0 0.0 0.0 3.1213085871023285 0.0 57 0.0 0.0 0.0 3.2444391054517787 0.0 58 0.0 0.0 0.0 3.384087132360302 0.0 59 0.0 0.0 0.0 3.52048171061325 0.0 60 0.0 0.0 0.0 3.6538731054918214 0.0 61 0.0 0.0 0.0 3.7940216629627406 0.0 62 0.0 0.0 0.0 3.938674995495225 0.0 63 0.0 0.0 0.0 4.074068512623381 0.0 64 0.0 0.0 0.0 4.222726089655034 0.0 65 0.0 0.0 0.0 4.364376238813142 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCCG 15 0.0021005985 71.01353 46 CTCGAGT 15 0.0021026866 70.99574 56 GATCCGA 25 2.225331E-4 56.81082 47 ATACGAA 25 2.2267087E-4 56.80371 16 AGGGCCG 25 2.2267087E-4 56.80371 19 CAATACG 25 2.2267087E-4 56.80371 14 CCGATCC 20 0.006571224 53.246807 45 GGCCGTG 20 0.006571224 53.246807 21 GGGCCGT 30 5.476275E-4 47.336422 20 AATACGA 30 5.476275E-4 47.336422 15 CGCCAGG 30 5.476275E-4 47.336422 15 GTGGGCC 30 5.4796576E-4 47.330498 25 CGCAATA 30 5.483041E-4 47.324574 12 GCGCAAT 30 5.4864265E-4 47.31865 11 TCAGCGC 30 5.4932025E-4 47.306805 6 CTCAGCG 30 5.4932025E-4 47.306805 5 TACGAAT 35 0.0011698671 40.574078 17 GCGCTCC 35 0.0011720306 40.558838 9 AGCGCTC 35 0.0011727525 40.553764 8 CAGCGCT 35 0.0011734747 40.54869 7 >>END_MODULE