FastQCFastQC Report
Thu 26 May 2016
SRR936172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399576
Sequences flagged as poor quality0
Sequence length125
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211995.305373696117885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150073.7557310749394355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136743.4221274551024083No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134993.3783310308927463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121023.0287104330590426No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58681.4685566700702746No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53751.3451758864396262No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24860.6221594890584019No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23070.5773620037239474No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20270.5072877249884878No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.4131879792580135No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9390.23499909904498767No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.2032154083328328No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.17668728852583737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6790.1699301259334895No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.1691793300898953No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.15866818827957635No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5510.13789616994013656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5220.13063847678539253No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT4640.11612309047590447No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4460.1116183154143392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGATC150.00422488659.53977100-101
CGCCGTA201.768546E-459.52113398-99
CTTACGC405.711627E-1059.52113398-99
ACCGGTA650.059.5211398-99
ACACGTA452.3646862E-1159.5211398-99
AACGGTA257.4342515E-659.5211398-99
GTTCGTC150.00423012759.5211398-99
GTCGTAC150.00423012759.5211398-99
CCGCGTA150.00423012759.5211398-99
GCCCGTA501.8189894E-1259.5211398-99
TACCGGT257.4342515E-659.5211398-99
TAAACCG201.7690937E-459.51740396-97
GACCCGT150.004231176359.51740396-97
CGAGCGA201.7690937E-459.51740396-97
ACATCGT303.1455966E-759.51740396-97
TTATTCG150.004231176359.51740396-97
CCGCTCG150.004237473559.4950668-69
TTACGCA5400.058.43718100-101
CCGTACA2450.058.32467100-101
CCCGTAT1800.057.86776798-99
Produced by FastQC (version 0.11.5)