FastQCFastQC Report
Thu 26 May 2016
SRR936171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682734
Sequences flagged as poor quality0
Sequence length77
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313324.589195792211901No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA204062.9888653560537484No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG12260.17957213204556974No Hit
GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC8750.12816118722665049No Hit
AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG7940.11629712303766913No Hit
TAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC7280.1066301077725732No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGG200.00656184153.2701265
TTACGTA200.006565646753.2623146
AACCGTC200.00657326453.246719
CGGTATA604.594703E-429.57283410
CGTTAGG500.00673289428.39408129
CGTCAAC1154.2831743E-827.7727474
CGCGTCA1154.2862666E-827.7707142
ACCGGGT851.1886356E-425.05727236
GCGTCAA1301.4057332E-724.56643
TGCGGAA750.001687011223.66693361
ATTTGCG3400.021.9170843
TCGCGAT1153.6916186E-521.60735726
TCGCGTC1708.029747E-820.8781
GCGGTAT850.00349741720.8749439
TTCGCGA1054.9673294E-420.28445825
GCCAATC1407.3004976E-620.28297257
TTGCGCG3700.020.1414995
ATACTCG900.0048590319.72244623
CGATTAT950.00664079918.68442360
ACTATCA950.006646419418.68168439