##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936171_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 682734 Sequences flagged as poor quality 0 Sequence length 77 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.902461280674466 40.0 36.0 41.0 31.0 41.0 2 36.71987626220461 39.0 36.0 41.0 31.0 41.0 3 36.59476018478646 39.0 35.0 41.0 30.0 41.0 4 36.457182445872036 39.0 35.0 41.0 30.0 41.0 5 36.286846707502484 39.0 35.0 41.0 30.0 41.0 6 36.02338538874584 39.0 35.0 40.0 30.0 41.0 7 35.64831984345294 38.0 35.0 40.0 30.0 41.0 8 35.27771870157338 38.0 35.0 40.0 29.0 41.0 9 35.02152229125838 38.0 35.0 40.0 28.0 41.0 10-11 35.00468337595608 38.0 35.0 40.0 27.5 41.0 12-13 34.83411255335167 38.0 35.0 40.0 26.0 41.0 14-15 34.551481250384484 38.0 34.5 40.0 24.5 41.0 16-17 34.1228860727604 38.0 34.0 40.0 22.0 41.0 18-19 33.63110010633717 38.0 33.0 40.0 17.5 41.0 20-21 32.90362864600269 37.0 33.0 40.0 5.0 41.0 22-23 32.014529084533656 37.0 32.5 40.0 2.0 41.0 24-25 31.318960971622914 37.0 31.0 40.0 2.0 41.0 26-27 30.242719712215887 35.5 29.0 39.0 2.0 40.5 28-29 30.181650540327567 35.0 29.5 39.0 2.0 40.5 30-31 30.418599337370047 35.0 30.0 40.0 2.0 41.0 32-33 30.199243775760397 35.0 29.5 40.0 2.0 41.0 34-35 29.89651679863607 35.0 29.0 39.5 2.0 41.0 36-37 29.581144340255502 35.0 28.0 39.0 2.0 41.0 38-39 29.266904094420376 35.0 28.0 39.0 2.0 41.0 40-41 28.859842193299293 35.0 26.5 38.5 2.0 40.5 42-43 28.48923299557368 35.0 26.0 38.0 2.0 40.0 44-45 28.10423239504697 35.0 26.0 37.0 2.0 40.0 46-47 27.71662829154546 35.0 25.0 37.0 2.0 39.5 48-49 27.282380839389866 34.0 24.0 36.0 2.0 39.0 50-51 26.84889210146265 34.0 22.5 35.5 2.0 39.0 52-53 26.41280059291027 34.0 20.0 35.0 2.0 38.0 54-55 25.949784982145317 34.0 16.5 35.0 2.0 37.0 56-57 25.49077752096717 33.0 9.0 35.0 2.0 37.0 58-59 25.03824183356915 33.0 3.5 35.0 2.0 36.0 60-61 24.648019580100012 33.0 2.0 35.0 2.0 36.0 62-63 24.249018651480664 33.0 2.0 35.0 2.0 35.0 64-65 23.826644784059383 33.0 2.0 35.0 2.0 35.0 66-67 23.374273875330655 32.0 2.0 35.0 2.0 35.0 68-69 22.94865057255095 32.0 2.0 35.0 2.0 35.0 70-71 22.469535719621405 31.0 2.0 35.0 2.0 35.0 72-73 21.96338105323596 31.0 2.0 35.0 2.0 35.0 74-75 21.39097950299824 31.0 2.0 34.5 2.0 35.0 76-77 20.089143063037728 28.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5210248240432662 1101 2 0.6842643898015623 1101 3 0.7068487538601076 1101 4 0.615585902784666 1101 5 0.6414319535641653 1101 6 0.4466184192580869 1101 7 0.5491634941845049 1101 8 0.6084404959714007 1101 9 0.5661059067861274 1101 10-11 0.5551271875552075 1101 12-13 0.36075244778746907 1101 14-15 0.4264644331999037 1101 16-17 0.5273980195650054 1101 18-19 0.7198187065485868 1101 20-21 0.7537103737113995 1101 22-23 0.4854888041483427 1101 24-25 0.3489648257071494 1101 26-27 0.34314889880759125 1101 28-29 0.3365833256158304 1101 30-31 0.6355432010244542 1101 32-33 0.57936616879919 1101 34-35 0.6464599741069748 1101 36-37 0.619957074577453 1101 38-39 0.6802067210639784 1101 40-41 0.6182902839337778 1101 42-43 0.5273224371613203 1101 44-45 0.5046777746977007 1101 46-47 0.6357645079942067 1101 48-49 0.6925281415148241 1101 50-51 0.7313791826860729 1101 52-53 0.7017589832259254 1101 54-55 0.8763531389448005 1101 56-57 0.8392735780050771 1101 58-59 0.7984471773326653 1101 60-61 0.7290199343625794 1101 62-63 0.7118574648293432 1101 64-65 0.7414954225871178 1101 66-67 0.8757740514386292 1101 68-69 0.9705145724156701 1101 70-71 0.9016786155548715 1101 72-73 1.231867031446356 1101 74-75 1.0754873919031276 1101 76-77 0.9127263254904996 1102 1 0.3704452023226992 1102 2 0.273068538672824 1102 3 0.5090701749039823 1102 4 0.42247757730904567 1102 5 0.5683358306510797 1102 6 0.3866428243831592 1102 7 0.3594802061485609 1102 8 0.4338495591473972 1102 9 0.6525222360334553 1102 10-11 0.47323412637599205 1102 12-13 0.5175581497121513 1102 14-15 0.6186783663076767 1102 16-17 0.5246482830294283 1102 18-19 0.5629977514207383 1102 20-21 0.5846785361164137 1102 22-23 0.7384950718281935 1102 24-25 0.9230940064714694 1102 26-27 0.6503525706695896 1102 28-29 0.497591978341994 1102 30-31 0.5940558750496407 1102 32-33 0.44841741956184933 1102 34-35 0.6552111527635844 1102 36-37 0.7477900104030688 1102 38-39 0.6908659367720027 1102 40-41 0.684805343005273 1102 42-43 0.6842889910467242 1102 44-45 0.4300577181221854 1102 46-47 0.4859544797064501 1102 48-49 0.36170419121253516 1102 50-51 0.44913252452422014 1102 52-53 0.26741431630041745 1102 54-55 0.4378526674821579 1102 56-57 0.5450684085558564 1102 58-59 0.4390553641428063 1102 60-61 0.4715375448789736 1102 62-63 0.5387391554389183 1102 64-65 0.4338286080106002 1102 66-67 0.6111212698090291 1102 68-69 0.2098234636464653 1102 70-71 0.2650515147728001 1102 72-73 0.299684159406862 1102 74-75 0.06538283812865231 1102 76-77 0.3064073394125124 1103 1 0.7721537604663169 1103 2 0.6237177469019741 1103 3 0.33849948577822175 1103 4 0.7026679532357036 1103 5 0.6830000408541821 1103 6 0.8448061792105506 1103 7 0.9470136535319895 1103 8 0.5705671097022957 1103 9 0.4430977503555127 1103 10-11 0.48888859845565236 1103 12-13 0.6368611281742247 1103 14-15 0.6739378302722372 1103 16-17 0.8197858011239063 1103 18-19 0.8564848330542887 1103 20-21 0.9571782071257573 1103 22-23 0.84604760066971 1103 24-25 0.5241160707781809 1103 26-27 0.35074626282206367 1103 28-29 0.2837096692662371 1103 30-31 0.6705078204810562 1103 32-33 0.48392647777126285 1103 34-35 0.4484734241150754 1103 36-37 0.5810465792498469 1103 38-39 0.4293951292096061 1103 40-41 0.5059989211076541 1103 42-43 0.42921705175646707 1103 44-45 0.32445171733260736 1103 46-47 0.1521057276776716 1103 48-49 0.1233011118599876 1103 50-51 0.25734742122115506 1103 52-53 0.18479927923413442 1103 54-55 0.4221817458696613 1103 56-57 0.25111525682536495 1103 58-59 0.17251810046775873 1103 60-61 0.1607949033033229 1103 62-63 -0.05098756298894713 1103 64-65 0.14855532643846558 1103 66-67 0.36898962968094295 1103 68-69 0.25366695182473364 1103 70-71 0.11772949871805594 1103 72-73 -0.09165022669657219 1103 74-75 -0.6401705241558595 1103 76-77 -0.5719209493974198 1104 1 0.23640044520666237 1104 2 0.0999907855922686 1104 3 0.014082151856442238 1104 4 0.13373051759692345 1104 5 0.3569749898906025 1104 6 0.014084072575826667 1104 7 0.15578569396659958 1104 8 0.3737058357178853 1104 9 0.0638604176946842 1104 10-11 0.3046342429085058 1104 12-13 0.13616234919854264 1104 14-15 0.1465409658458583 1104 16-17 0.3295345559312963 1104 18-19 0.50803055316441 1104 20-21 0.435584360583924 1104 22-23 0.8687579173794582 1104 24-25 0.9379114388718257 1104 26-27 0.44513396431209173 1104 28-29 0.5731250066766584 1104 30-31 0.7409111157755923 1104 32-33 0.583970085782525 1104 34-35 0.5951618422476059 1104 36-37 0.5312283668630648 1104 38-39 0.6524290909186199 1104 40-41 0.6579597054213799 1104 42-43 0.6983861877924475 1104 44-45 0.8858050367954426 1104 46-47 1.079382801422497 1104 48-49 1.1391891390966045 1104 50-51 0.9942807797735789 1104 52-53 1.09703947005859 1104 54-55 0.8571296264152366 1104 56-57 1.0306609979513972 1104 58-59 1.0697686941671911 1104 60-61 0.9047633374079389 1104 62-63 0.8402130758946882 1104 64-65 0.640795202024286 1104 66-67 0.804670440054398 1104 68-69 0.9057275617064171 1104 70-71 1.039550317077552 1104 72-73 0.44951205815305784 1104 74-75 0.491953869009766 1104 76-77 0.33434546928152287 1105 1 0.25492215699422616 1105 2 0.1422225199994358 1105 3 0.10895267231045835 1105 4 0.36649447227654264 1105 5 0.13168666587222333 1105 6 -0.12232336451020132 1105 7 -0.04906890146687459 1105 8 -0.05180665578707533 1105 9 0.14065553266927822 1105 10-11 0.068173046199 1105 12-13 0.1670563818803359 1105 14-15 0.3054995506491309 1105 16-17 0.623003776011025 1105 18-19 0.5971396649134206 1105 20-21 0.36167502162442844 1105 22-23 0.24206778582052735 1105 24-25 0.24026367598219522 1105 26-27 0.026077699287291978 1105 28-29 0.1465423943646904 1105 30-31 0.09375682565620735 1105 32-33 0.34528242919494545 1105 34-35 0.6863731581411798 1105 36-37 0.47888373724658706 1105 38-39 0.46030944025473985 1105 40-41 0.3701048158314748 1105 42-43 0.44819346461393295 1105 44-45 0.32359348760538253 1105 46-47 0.22747788495278343 1105 48-49 0.2754165321606017 1105 50-51 0.3969682537813064 1105 52-53 0.40973536302622193 1105 54-55 0.30433108299673606 1105 56-57 0.4864944242652136 1105 58-59 0.439391463903565 1105 60-61 0.15345484845909496 1105 62-63 0.07080889481604657 1105 64-65 0.08196391595208397 1105 66-67 0.0643928172440873 1105 68-69 -0.15535554255682626 1105 70-71 -0.450062917933149 1105 72-73 -0.46637188192017476 1105 74-75 -0.4977430484337084 1105 76-77 -0.2535692659269735 1106 1 0.08066001086291408 1106 2 0.09903343952206711 1106 3 -0.23410981684250487 1106 4 -0.22957711307561368 1106 5 -0.39769987665327733 1106 6 -0.2510279473203241 1106 7 0.012246219050105367 1106 8 0.06560169311494946 1106 9 0.06456802131179273 1106 10-11 -0.05409477612394653 1106 12-13 0.2971187587345412 1106 14-15 0.528267102434171 1106 16-17 0.9109479941564658 1106 18-19 0.6399101773020206 1106 20-21 0.24996328232088416 1106 22-23 0.39778787606789834 1106 24-25 0.23095819274023555 1106 26-27 0.3624379945309286 1106 28-29 0.5960232678448278 1106 30-31 0.6183630143385344 1106 32-33 1.008904268240201 1106 34-35 0.7495156856798957 1106 36-37 0.5391498852971814 1106 38-39 0.40582923035815455 1106 40-41 0.5713901261333554 1106 42-43 0.5344822970131453 1106 44-45 0.4496896765939802 1106 46-47 0.6250180317578824 1106 48-49 0.3895299334857256 1106 50-51 0.12033047852174406 1106 52-53 0.22986083136307656 1106 54-55 0.4854868413706477 1106 56-57 0.4111904519519598 1106 58-59 0.544773636986573 1106 60-61 0.6665376534778282 1106 62-63 0.6159963826928845 1106 64-65 0.5924128047048534 1106 66-67 0.7333845785990221 1106 68-69 0.7432284722257769 1106 70-71 0.641715792264602 1106 72-73 0.8293235026009711 1106 74-75 0.5642126854386582 1106 76-77 1.1195331403290858 1107 1 0.1144629648498281 1107 2 -0.0348060134793684 1107 3 -0.16055943603612377 1107 4 -0.283736784097826 1107 5 -0.010043843366176475 1107 6 -0.13437953016092052 1107 7 0.2093262978879764 1107 8 0.3182770708797449 1107 9 0.09288152351389556 1107 10-11 0.4933052774079343 1107 12-13 0.34490083528513793 1107 14-15 0.23413857723768672 1107 16-17 0.41675985390291714 1107 18-19 0.4660872705205037 1107 20-21 0.5802063657576326 1107 22-23 0.6033661421213772 1107 24-25 0.5554210489097855 1107 26-27 0.8427139270807515 1107 28-29 0.9026368892770478 1107 30-31 1.1240288780268806 1107 32-33 1.1043832569741738 1107 34-35 1.0179817537627258 1107 36-37 1.052654520672025 1107 38-39 1.0333105550290576 1107 40-41 0.8481852693311538 1107 42-43 1.1185297943973858 1107 44-45 1.0402522480712193 1107 46-47 1.0065592634779605 1107 48-49 1.0175514435716195 1107 50-51 1.0746775111518652 1107 52-53 1.143808321447512 1107 54-55 1.061278261668967 1107 56-57 0.8054017082480378 1107 58-59 0.7232343518806061 1107 60-61 0.7724736098736713 1107 62-63 0.702368867302468 1107 64-65 0.6542214525380281 1107 66-67 0.5375089672405302 1107 68-69 0.24416771353292788 1107 70-71 0.20423252097959121 1107 72-73 0.17954614658958334 1107 74-75 0.36744041495662927 1107 76-77 0.20912848792136884 1108 1 0.07603477759336386 1108 2 0.0770978337298942 1108 3 0.016675622412918756 1108 4 -0.04582448510216608 1108 5 -0.08115958893447583 1108 6 -0.07866359799473344 1108 7 -0.2878033876397055 1108 8 -0.045007051835803225 1108 9 0.08592252016611468 1108 10-11 -0.05457903190549018 1108 12-13 -0.0018583985659077484 1108 14-15 -0.18744640712102267 1108 16-17 0.11119484649388056 1108 18-19 0.3359132954368036 1108 20-21 0.020610295289493763 1108 22-23 0.0327168912569924 1108 24-25 0.20890329467016855 1108 26-27 0.038449616669687714 1108 28-29 0.26782540801916355 1108 30-31 0.13134461261883246 1108 32-33 0.22415714424710131 1108 34-35 0.21081419984142613 1108 36-37 0.395127491420066 1108 38-39 0.3740053577292386 1108 40-41 0.42292320907101555 1108 42-43 0.2675227146542305 1108 44-45 0.15340191054857755 1108 46-47 0.0064769408831466535 1108 48-49 0.25387217240099247 1108 50-51 0.15628115529872844 1108 52-53 -0.028240413528614283 1108 54-55 -0.11055643456066022 1108 56-57 -0.06296446265366384 1108 58-59 -0.17287453347146098 1108 60-61 -0.2840765245865633 1108 62-63 -0.00845380982831756 1108 64-65 0.0020069747376894043 1108 66-67 -0.22436469501730372 1108 68-69 -0.34290763908218835 1108 70-71 -0.5103440664744454 1108 72-73 -0.4485093937210465 1108 74-75 -0.6745880137794451 1108 76-77 -0.622587663771732 1109 1 0.019639934423075545 1109 2 0.23885097968226887 1109 3 0.12746378944840586 1109 4 0.5464336646484753 1109 5 -0.03331469016643496 1109 6 0.27102538484152205 1109 7 0.1923630753519987 1109 8 0.18733432941068884 1109 9 0.12497306458377011 1109 10-11 -6.161778138249474E-4 1109 12-13 0.3590833503964532 1109 14-15 0.4092947935703677 1109 16-17 0.16650525813748374 1109 18-19 -0.07363080294370405 1109 20-21 -0.26454124988782013 1109 22-23 -0.08940991567015999 1109 24-25 -0.41021022384223116 1109 26-27 -0.4834669096264008 1109 28-29 -0.28931697728982186 1109 30-31 -0.35010555307349733 1109 32-33 -0.05082716727650194 1109 34-35 -0.03964874783062555 1109 36-37 -0.2343128978743394 1109 38-39 -0.3655395113132691 1109 40-41 -0.3246948948802775 1109 42-43 -0.47167535648888403 1109 44-45 -0.35824059035969924 1109 46-47 -0.48219542868231 1109 48-49 -0.5079206075970255 1109 50-51 -0.5861669075872946 1109 52-53 -0.31224697516505984 1109 54-55 -0.20332101933898272 1109 56-57 -0.15895261647780146 1109 58-59 -0.33772574065843486 1109 60-61 -0.5150472284211602 1109 62-63 -0.8354269797813068 1109 64-65 -0.7710022401779248 1109 66-67 -0.8630365141099041 1109 68-69 -1.1231555720566142 1109 70-71 -0.8358904107842058 1109 72-73 -0.8591841633481572 1109 74-75 -0.9235415648798408 1109 76-77 -0.9490577015341692 1110 1 -0.2916739991818744 1110 2 -0.43630977287786266 1110 3 -0.341538911752437 1110 4 -0.14384651789177383 1110 5 -0.13075101670492728 1110 6 -0.2337902190452894 1110 7 -0.31601408008397414 1110 8 -0.1447588974193792 1110 9 -0.11089819605529527 1110 10-11 -0.0651178073309353 1110 12-13 0.04026763065193961 1110 14-15 -0.0773021868346575 1110 16-17 -0.33721291585932534 1110 18-19 -0.5675847473620408 1110 20-21 -0.4713075541773364 1110 22-23 -0.38681877964249267 1110 24-25 -0.2858916924029593 1110 26-27 0.01966983035244496 1110 28-29 -0.027600867276497354 1110 30-31 0.04817031269463001 1110 32-33 -0.005594389845580139 1110 34-35 0.05136927662393376 1110 36-37 -0.00363485140681874 1110 38-39 0.09634652332818305 1110 40-41 0.3179881549235155 1110 42-43 0.29963119655142023 1110 44-45 -0.0026861790777346073 1110 46-47 0.007758206782163768 1110 48-49 -0.05873386429263405 1110 50-51 -0.058811617074109535 1110 52-53 0.17215621821646465 1110 54-55 0.04941075506461701 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7 -0.6673654314353286 2314 8 -0.4879722480698021 2314 9 -0.44522279525403974 2314 10-11 -0.5175052634369237 2314 12-13 -0.5890908468186709 2314 14-15 -0.6429490694151809 2314 16-17 -1.328551794784012 2314 18-19 -0.9935791055646419 2314 20-21 -0.7349928642013239 2314 22-23 -0.5082789125442737 2314 24-25 -0.45556565698538876 2314 26-27 -0.6370343566721104 2314 28-29 -0.4839800153032421 2314 30-31 -0.489054555193853 2314 32-33 -0.6384094131068281 2314 34-35 -0.8298016540321242 2314 36-37 -0.8176462312076715 2314 38-39 -0.5612982495911893 2314 40-41 -0.4579823235641278 2314 42-43 -0.14090230033219697 2314 44-45 -0.2863041792289174 2314 46-47 -0.40604289408987526 2314 48-49 -0.16104813508516358 2314 50-51 -0.06675033382289186 2314 52-53 0.16611499392580953 2314 54-55 0.23246165493473825 2314 56-57 0.3976767799853569 2314 58-59 0.2123021300216088 2314 60-61 0.16473778480375145 2314 62-63 0.2727549775055138 2314 64-65 0.24268718207927265 2314 66-67 0.06169352283241025 2314 68-69 0.251571185982165 2314 70-71 0.34797956976096245 2314 72-73 0.6853105576171536 2314 74-75 0.4994643152871667 2314 76-77 0.6218841088577669 2315 1 -0.4924803658734689 2315 2 -0.5751858212170333 2315 3 -0.5336087468916801 2315 4 -0.5793434293181576 2315 5 -0.5178409396199868 2315 6 -0.47905741055827633 2315 7 -0.4001132357013262 2315 8 -0.5728239564690512 2315 9 -0.44446057703232356 2315 10-11 -0.6653960555983573 2315 12-13 -0.6796894987683118 2315 14-15 -0.8800216665336009 2315 16-17 -0.8314953512366614 2315 18-19 -0.629291074966801 2315 20-21 -1.0126842673496057 2315 22-23 -1.0258750914518906 2315 24-25 -1.2851144177367573 2315 26-27 -1.0487276630929259 2315 28-29 -1.0383702710327576 2315 30-31 -1.196329420479735 2315 32-33 -1.199007277077392 2315 34-35 -1.0920615477155629 2315 36-37 -1.078938577506289 2315 38-39 -0.9862864299050464 2315 40-41 -1.087335150287526 2315 42-43 -1.1917625613351106 2315 44-45 -1.1708523641494217 2315 46-47 -1.1284121047166806 2315 48-49 -1.0199566931440707 2315 50-51 -0.994669947834371 2315 52-53 -1.0612510647846918 2315 54-55 -1.0782006111718552 2315 56-57 -1.115907524683724 2315 58-59 -0.9104021232469854 2315 60-61 -1.1122982319014056 2315 62-63 -1.2061999197844457 2315 64-65 -1.0955422289366759 2315 66-67 -1.16378441678415 2315 68-69 -1.4172722728822968 2315 70-71 -1.3651536168870315 2315 72-73 -2.3471026586103676 2315 74-75 -3.107102278036834 2315 76-77 -2.6077958807166617 2316 1 -2.6000850084589757 2316 2 -2.7141245630177693 2316 3 -2.1761236630593785 2316 4 -2.2039465913162957 2316 5 -3.0369676930748355 2316 6 -2.387571582020527 2316 7 -2.6997142359360353 2316 8 -2.4584516708056867 2316 9 -1.9624826252546512 2316 10-11 -2.3206071407683027 2316 12-13 -2.4100487750275548 2316 14-15 -2.5041977699594185 2316 16-17 -3.8594253348072556 2316 18-19 -3.715824259359046 2316 20-21 -3.1749885836411806 2316 22-23 -3.0905268181273478 2316 24-25 -2.8645349592540867 2316 26-27 -2.467508887339264 2316 28-29 -2.74981808782816 2316 30-31 -2.741546452705016 2316 32-33 -2.784078403506143 2316 34-35 -2.6215294715579596 2316 36-37 -2.4241863155958576 2316 38-39 -2.7366406944025385 2316 40-41 -2.703503023129958 2316 42-43 -2.688026681179746 2316 44-45 -2.617810630644559 2316 46-47 -2.434340454170595 2316 48-49 -2.451614086286778 2316 50-51 -2.7013892379994964 2316 52-53 -3.6742124689250417 2316 54-55 -2.931865991368692 2316 56-57 -2.8226726971747986 2316 58-59 -2.581856434156215 2316 60-61 -2.4301912801996117 2316 62-63 -2.542569427802384 2316 64-65 -2.1098628087480975 2316 66-67 -2.464716004471452 2316 68-69 -2.461440188077642 2316 70-71 -2.1412884544547737 2316 72-73 -2.0847316364756736 2316 74-75 -1.9691500062440532 2316 76-77 -1.8060878228978758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16134.0 3 2753.0 4 5097.0 5 6144.0 6 6834.0 7 6789.0 8 8731.0 9 10237.0 10 15591.0 11 17483.0 12 10939.0 13 6236.0 14 5524.0 15 5271.0 16 5055.0 17 5004.0 18 5431.0 19 5565.0 20 5860.0 21 6193.0 22 6574.0 23 7524.0 24 8459.0 25 9498.0 26 10756.0 27 12116.0 28 14021.0 29 15877.0 30 18326.0 31 21475.0 32 26303.0 33 33225.0 34 49510.0 35 61007.0 36 67181.0 37 79155.0 38 66945.0 39 17735.0 40 176.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.504694801294917 25.77819444240995 38.08428669781886 17.632824058476277 2 18.41827706226342 26.464151363513842 37.95177016559047 17.165801408632266 3 18.27040551745152 26.417523885420298 37.737206174904856 17.574864422223328 4 18.44851201593824 26.488386912478852 37.711514938437084 17.351586133145823 5 18.188556637452045 26.619976005238417 37.63559310861072 17.555874248698817 6 18.22067664326814 26.7771553795132 37.86840964222517 17.133758334993495 7 18.18876990463355 26.95354730966995 37.16039435711879 17.697288428577707 8 17.631858054460118 27.40321229132119 36.87909080787779 18.085838846340906 9 17.774620032960996 27.17773301593115 37.25632668009522 17.791320271012633 10-11 18.038890520701102 27.289654318690726 37.10003611147532 17.57141904913285 12-13 18.282285520078286 27.375305437222032 36.17958829699339 18.1628207457063 14-15 18.14838061854432 27.696655917420003 36.13057881141115 18.024384652624533 16-17 18.07750504986883 27.958769047782965 35.81268831726213 18.151037585086076 18-19 18.151733473187054 28.22918528371651 35.02171053209848 18.59737071099795 20-21 18.221261153560754 28.44184545693614 34.53935729998308 18.79753608952003 22-23 18.613596093176024 28.9586661597816 33.28601429386588 19.141723453176493 24-25 18.71018070215275 29.65756685686661 31.6374530298924 19.99479941108824 26-27 19.09759763719311 30.104325073471454 30.973969275306267 19.824108014029164 28-29 19.186258145365244 30.378577753494167 30.401722053075154 20.033442048065435 30-31 18.87913728184309 30.29247252920294 30.422108764068646 20.40628142488532 32-33 19.28185333841151 30.65003076201916 29.76027597925761 20.30783992031172 34-35 19.148710203597325 30.131554179212817 30.315251012053825 20.404484605136037 36-37 19.3822551619729 30.380254899763496 30.468366047251894 19.769123891011713 38-39 19.802296385492753 30.205467612409503 30.560194422226715 19.43204157987103 40-41 19.58895330313229 30.58174240731856 30.536257731845502 19.293046557703647 42-43 19.52184931807861 30.79515989628349 30.17286084701815 19.510129938619748 44-45 19.80206504518797 30.61809994455419 30.21254716006847 19.36728785018937 46-47 19.319403789750037 30.9686951527884 30.153037455060083 19.558863602401484 48-49 19.328666738941255 31.016786261577362 29.843740385770502 19.81080661371088 50-51 18.826327950663664 31.376456229239896 29.932717850136044 19.864497969960397 52-53 18.269347708547322 31.29942517704049 30.40979030610825 20.021436808303942 54-55 18.16664786470794 31.208638743071965 30.348632267384364 20.276081124835727 56-57 18.24535907429803 31.238779042639454 30.114394249595843 20.401467633466673 58-59 18.270199874602273 31.023485705245022 29.658906461498795 21.047407958653906 60-61 18.506277560468217 30.51604916567779 29.65770081600961 21.319972457844386 62-63 18.305603231357175 30.55753950376633 29.61095813344292 21.52589913143358 64-65 18.3672856440659 30.078433756916155 29.816328608652398 21.73795199036555 66-67 18.38139254128125 30.28312481182518 29.723307012666595 21.612175634226976 68-69 18.53292744130501 29.92882236362504 29.87651064733917 21.661739547730775 70-71 18.50642284923762 29.55287355732277 30.094809476111518 21.8458941173281 72-73 18.9052359682004 29.173917058933046 30.057670853936173 21.863176118930376 74-75 18.870877183723792 28.96947734292655 30.01792863435994 22.141716838989723 76-77 19.14322176587914 28.548181497653985 30.636836831407088 21.67175990505979 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 40916.0 1 23492.5 2 5386.0 3 4555.5 4 4408.0 5 4224.0 6 4044.0 7 4089.0 8 4026.5 9 3923.0 10 3872.5 11 3853.5 12 4036.5 13 4188.0 14 4335.0 15 4619.5 16 4961.0 17 5165.0 18 5436.0 19 5913.0 20 6387.0 21 7106.0 22 7557.0 23 7869.5 24 8612.5 25 9556.0 26 10069.0 27 10368.0 28 11409.5 29 12571.5 30 12991.0 31 13819.0 32 15287.0 33 16485.5 34 17142.0 35 17240.0 36 17911.0 37 19161.0 38 19520.0 39 19300.0 40 19640.0 41 20399.5 42 21330.0 43 21841.0 44 21284.0 45 21201.0 46 21855.0 47 22206.0 48 22377.0 49 22590.5 50 22816.5 51 22220.5 52 21612.0 53 21359.5 54 20312.5 55 18706.0 56 17299.5 57 16705.0 58 16988.0 59 15872.5 60 13727.5 61 12981.0 62 11858.5 63 8471.5 64 5247.0 65 4287.0 66 3853.0 67 3082.0 68 2432.0 69 1828.0 70 1537.0 71 1344.0 72 993.0 73 843.0 74 851.0 75 799.5 76 548.0 77 301.0 78 254.0 79 214.5 80 156.5 81 107.5 82 76.0 83 75.0 84 63.0 85 38.0 86 27.0 87 29.0 88 23.0 89 15.5 90 12.0 91 10.0 92 11.5 93 9.0 94 8.0 95 11.0 96 11.0 97 9.5 98 8.0 99 15.5 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009374075408577865 2 0.012596413830276505 3 0.014646992825902915 4 0.014500522897643885 5 0.012449943902017476 6 0.01025289497813204 7 0.015232872538939032 8 0.015525812395457089 9 0.015965222180234176 10-11 0.018381975996508156 12-13 0.015525812395457089 14-15 0.007396731377080972 16-17 0.006298206915138252 18-19 0.016990511678047382 20-21 0.02350842348557418 22-23 0.01955373542258039 24-25 0.017429921462824467 26-27 0.022849308808408547 28-29 0.008861430659671264 30-31 0.007982611090117088 32-33 0.010838774691168156 34-35 0.01281611872266505 36-37 0.010765539727038642 38-39 0.014061113112866798 40-41 0.012669648794406019 42-43 0.015232872538939032 44-45 0.01252317886614699 46-47 0.022849308808408547 48-49 0.02226342909537243 50-51 0.03442043314087185 52-53 0.038521591132124666 54-55 0.026511057014884272 56-57 0.020286085063875536 58-59 0.015525812395457089 60-61 0.021091669669300196 62-63 0.025266062624682527 64-65 0.026877231835531847 66-67 0.027902521333345053 68-69 0.041963634446211845 70-71 0.05353475877867515 72-73 0.0859778478880501 74-75 0.08568490803153204 76-77 0.09088459048472758 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 682734.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.670295724240226 #Duplication Level Percentage of deduplicated Percentage of total 1 81.88381857085258 46.403802134402504 2 9.301224088485087 10.542062393837535 3 3.5257569027280584 5.994170589881411 4 1.716709764572873 3.891458001241422 5 0.9959014757545888 2.8219015571609907 6 0.5931722351293057 2.016914758811179 7 0.3840185863984655 1.5233712798364014 8 0.269094810616965 1.2199745996417461 9 0.2038612226505222 1.0397588196879246 >10 1.0318378435287539 10.39856851415859 >50 0.05867549792135759 2.2849833793792977 >100 0.034119627192184546 3.6597194670186797 >500 0.0010339280967283269 0.44501391853888717 >1k 2.584820241820817E-4 0.1795875787125331 >5k 0.0 0.0 >10k+ 5.169640483641634E-4 7.5787130076908955 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31332 4.589195792211901 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20406 2.9888653560537484 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 1226 0.17957213204556974 No Hit GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 875 0.12816118722665049 No Hit AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG 794 0.11629712303766913 No Hit TAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC 728 0.1066301077725732 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.858797130361166E-4 0.0 2 0.0 0.0 0.0 0.0035152782782166994 0.0 3 0.0 0.0 0.0 0.004540567776029903 0.0 4 0.0 0.0 0.0 0.0077629061977285444 0.0 5 0.0 0.0 0.0 0.01669757182152932 0.0 6 0.0 0.0 0.0 0.02900104579528777 0.0 7 0.0 0.0 0.0 0.048920956038515734 0.0 8 0.0 0.0 0.0 0.07015909563607496 0.0 9 0.0 0.0 0.0 0.0940336939422967 0.0 10 0.0 0.0 0.0 0.1236206194506206 0.0 11 0.0 0.0 0.0 0.15642988338064312 0.0 12 0.0 0.0 0.0 0.19421912487147264 0.0 13 0.0 0.0 0.0 0.2447512501208377 0.0 14 0.0 0.0 0.0 0.3009957025723049 0.0 15 0.0 0.0 0.0 0.3749630163431146 0.0 16 0.0 0.0 0.0 0.4544961873877674 0.0 17 0.0 0.0 0.0 0.513084158691379 0.0 18 0.0 0.0 0.0 0.5748944684166893 0.0 19 0.0 0.0 0.0 0.6387553571376261 0.0 20 0.0 0.0 0.0 0.7067174038498156 0.0 21 0.0 0.0 0.0 0.7793664882662941 0.0 22 0.0 0.0 0.0 0.8580208397413926 0.0 23 0.0 0.0 0.0 0.9335993227230517 0.0 24 0.0 0.0 0.0 1.0145971930502948 0.0 25 0.0 0.0 0.0 1.1111208757729951 0.0 26 0.0 0.0 0.0 1.2126245360565022 0.0 27 0.0 0.0 0.0 1.3145676061247866 0.0 28 0.0 0.0 0.0 1.3992272246585054 0.0 29 0.0 0.0 0.0 1.4853515424748145 0.0 30 0.0 0.0 0.0 1.5748446686410813 0.0 31 0.0 0.0 0.0 1.6744442198572211 0.0 32 0.0 0.0 0.0 1.784882545764529 0.0 33 0.0 0.0 0.0 1.8985432100935358 0.0 34 0.0 0.0 0.0 2.0179162016246446 0.0 35 0.0 0.0 0.0 2.1397791819361567 0.0 36 0.0 0.0 0.0 2.282147952203933 0.0 37 0.0 0.0 0.0 2.4294967000325163 0.0 38 0.0 0.0 0.0 2.5712795905872565 0.0 39 0.0 0.0 0.0 2.7135018909267736 0.0 40 0.0 0.0 0.0 2.848693634709858 0.0 41 0.0 0.0 0.0 2.9935523937580375 0.0 42 0.0 0.0 0.0 3.1435376002952835 0.0 43 0.0 0.0 0.0 3.308755679371468 0.0 44 0.0 0.0 0.0 3.477342566797611 0.0 45 0.0 0.0 0.0 3.655010589775813 0.0 46 0.0 0.0 0.0 3.8534773425667974 0.0 47 0.0 0.0 0.0 4.060146411340288 0.0 48 0.0 0.0 0.0 4.267547829755073 0.0 49 0.0 0.0 0.0 4.475681597811153 0.0 50 0.0 0.0 0.0 4.67532011002821 0.0 51 0.0 0.0 0.0 4.896343231771085 0.0 52 0.0 0.0 0.0 5.108724627746677 0.0 53 0.0 0.0 0.0 5.34219769339157 0.0 54 0.0 0.0 0.0 5.60760120339693 0.0 55 0.0 0.0 0.0 5.857332431078575 0.0 56 0.0 0.0 0.0 6.149832877811856 0.0 57 0.0 0.0 0.0 6.436181587558258 0.0 58 0.0 0.0 0.0 6.74069256840878 0.0 59 0.0 0.0 0.0 7.038026522774609 0.0 60 0.0 0.0 0.0 7.329501680010077 0.0 61 0.0 0.0 0.0 7.616436269469515 1.4646992825902914E-4 62 0.0 0.0 0.0 7.925634288024326 1.4646992825902914E-4 63 0.0 0.0 0.0 8.223554122103192 1.4646992825902914E-4 64 0.0 0.0 0.0 8.538464467860104 1.4646992825902914E-4 65 0.0 0.0 0.0 8.891603464892622 1.4646992825902914E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTCGG 20 0.006561841 53.27012 65 TTACGTA 20 0.0065656467 53.26231 46 AACCGTC 20 0.006573264 53.2467 19 CGGTATA 60 4.594703E-4 29.572834 10 CGTTAGG 50 0.006732894 28.394081 29 CGTCAAC 115 4.2831743E-8 27.772747 4 CGCGTCA 115 4.2862666E-8 27.770714 2 ACCGGGT 85 1.1886356E-4 25.057272 36 GCGTCAA 130 1.4057332E-7 24.5664 3 TGCGGAA 75 0.0016870112 23.666933 61 ATTTGCG 340 0.0 21.917084 3 TCGCGAT 115 3.6916186E-5 21.607357 26 TCGCGTC 170 8.029747E-8 20.878 1 GCGGTAT 85 0.003497417 20.874943 9 TTCGCGA 105 4.9673294E-4 20.284458 25 GCCAATC 140 7.3004976E-6 20.282972 57 TTGCGCG 370 0.0 20.141499 5 ATACTCG 90 0.00485903 19.722446 23 CGATTAT 95 0.006640799 18.684423 60 ACTATCA 95 0.0066464194 18.681684 39 >>END_MODULE