FastQCFastQC Report
Thu 26 May 2016
SRR936171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682734
Sequences flagged as poor quality0
Sequence length125
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173542.5418391350071916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124551.824282956466208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103131.5105443701353674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50610.7412843069189464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33920.49682599665462684No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28860.42271221295555816No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23910.3502095984673387No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21390.3132991765460633No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20500.30026335293100975No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.25632237445330097No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11340.16609689864573904No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.1614098609414501No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10650.15599047359586604No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT9470.1387070220613006No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT7420.10868068676819961No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG7190.10531187841824195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6910.10121072042698913No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC6870.10062484071395301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6850.10033190085743497No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA201.7693924E-459.51841496-97
CGTATCA9200.055.012005100-101
ACCGGTA506.91216E-1153.58816598-99
AGTATCA22800.053.14421100-101
CCGTACA3300.052.32514100-101
TACGCAG7550.051.261677100-101
CGTACAT9600.050.859142100-101
CCCGTAC2000.050.61104698-99
AAGTATC17700.050.291466100-101
AGGTATC14300.050.173843100-101
CCGTATC3650.049.754612100-101
GGGTATC6450.049.387886100-101
AACGTAC850.049.0349298-99
TGAGTAC10250.048.21483100-101
ACCGTAT1000.047.63392698-99
CCCGGTA2500.047.63392398-99
CGGTATC8500.047.283676100-101
CGAGTAC6400.046.98268100-101
AACGTAT1150.046.59840498-99
ACGTACA2500.046.443073100-101
Produced by FastQC (version 0.11.5)