FastQCFastQC Report
Thu 26 May 2016
SRR936170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683783
Sequences flagged as poor quality0
Sequence length77
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308644.513712683702285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA203222.97199550149682No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG12100.1769567245749017No Hit
GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC8700.12723334742162354No Hit
AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG7600.11114637246026882No Hit
TAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC7130.10427284679496274No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCG400.002250176935.5192271
CGATCCG601.1024538E-535.50362446
TTCGCGA500.006727505528.39874325
TGACACG500.006734720428.392512
CGATTAT657.312624E-427.31447860
CGGTATA657.3282776E-427.30448510
GTGGGTA1809.094947E-1225.6321261
GCGCTCC1051.8288036E-523.6638879
GGCCGAC1201.9222334E-623.65696528
TAGCTTA800.002457991522.1832714
GATCCGA1003.569815E-421.30217447
ATGGCGG850.00349409320.8783726
TATATAG1205.1211508E-520.70893113
TCAGCGC1054.9716537E-420.2818496
ATTTGCG3850.020.2818473
ATCGATT900.00485443519.72567758
GAGTGGT950.006645748418.68201614
GTGCTAC950.006645748418.68201616
ACGTAGG950.00664855618.6806493
TAGTATG950.00664855618.6806495