##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936170_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 683783 Sequences flagged as poor quality 0 Sequence length 77 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.85963968100991 40.0 36.0 41.0 31.0 41.0 2 36.68959450585932 39.0 36.0 41.0 31.0 41.0 3 36.50669145035779 39.0 35.0 41.0 30.0 41.0 4 36.39231013347802 39.0 35.0 41.0 30.0 41.0 5 36.22448496087209 39.0 35.0 41.0 30.0 41.0 6 35.97608305558927 39.0 35.0 40.0 30.0 41.0 7 35.620574071013756 38.0 35.0 40.0 30.0 41.0 8 35.28708376780353 38.0 35.0 40.0 29.0 41.0 9 35.21279411743199 38.0 35.0 40.0 29.0 41.0 10-11 35.16047854363153 38.0 35.0 40.0 28.0 41.0 12-13 34.80104506839158 38.0 35.0 40.0 25.5 41.0 14-15 34.455294296582395 38.0 34.5 40.0 23.5 41.0 16-17 34.052665831118944 38.0 34.0 40.0 21.5 41.0 18-19 33.54974385148505 38.0 33.0 40.0 16.5 41.0 20-21 32.834813091287735 37.0 33.0 40.0 5.0 41.0 22-23 31.926464243773246 37.0 32.5 40.0 2.0 41.0 24-25 31.243883658997078 37.0 31.0 40.0 2.0 41.0 26-27 30.15131554893877 35.5 29.0 39.0 2.0 40.5 28-29 30.063390724835216 35.0 28.5 39.0 2.0 40.5 30-31 30.33716471453663 35.0 30.0 40.0 2.0 41.0 32-33 30.147864892809558 35.0 29.0 40.0 2.0 41.0 34-35 29.85810629980564 35.0 28.5 39.5 2.0 41.0 36-37 29.535859329641127 35.0 28.0 39.0 2.0 41.0 38-39 29.19585087666701 35.0 27.5 39.0 2.0 41.0 40-41 28.814576408012485 35.0 26.5 38.5 2.0 40.5 42-43 28.429034503636387 35.0 26.0 38.0 2.0 40.0 44-45 28.051094426155665 35.0 26.0 37.0 2.0 40.0 46-47 27.641616565489343 34.5 24.5 37.0 2.0 39.5 48-49 27.24256379582411 34.0 23.5 36.0 2.0 39.0 50-51 26.79597401514808 34.0 21.5 35.5 2.0 39.0 52-53 26.339623096801176 34.0 19.5 35.0 2.0 37.5 54-55 25.87643228919116 34.0 15.5 35.0 2.0 37.0 56-57 25.427611537578443 33.0 7.5 35.0 2.0 37.0 58-59 24.97211322890449 33.0 2.0 35.0 2.0 36.0 60-61 24.578743548757426 33.0 2.0 35.0 2.0 36.0 62-63 24.15849545835448 33.0 2.0 35.0 2.0 35.0 64-65 23.721854009239774 32.5 2.0 35.0 2.0 35.0 66-67 23.301074317436964 32.0 2.0 35.0 2.0 35.0 68-69 22.88139365851447 32.0 2.0 35.0 2.0 35.0 70-71 22.37783697459574 31.0 2.0 35.0 2.0 35.0 72-73 21.87396220730846 31.0 2.0 35.0 2.0 35.0 74-75 21.318146254001633 31.0 2.0 34.0 2.0 35.0 76-77 19.978262840696537 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.161434138983445 1101 2 1.2438681299082575 1101 3 1.3440150333439647 1101 4 1.2771610401539988 1101 5 1.0271187224526486 1101 6 1.3019916308641442 1101 7 1.407446138604456 1101 8 1.3120677897722999 1101 9 1.2016573272377968 1101 10-11 1.364944898686808 1101 12-13 1.7350417369536402 1101 14-15 1.9868612432885584 1101 16-17 1.9648003868588404 1101 18-19 2.011566145895536 1101 20-21 2.0721860975104036 1101 22-23 1.3557713146617232 1101 24-25 1.454547081565888 1101 26-27 1.3025955215581781 1101 28-29 1.4252440487933171 1101 30-31 1.622868691706973 1101 32-33 1.6586435334403689 1101 34-35 1.5394706488872707 1101 36-37 1.5277796184719712 1101 38-39 1.4529349174896744 1101 40-41 1.284403846452431 1101 42-43 1.3930957849024317 1101 44-45 1.3830220025166682 1101 46-47 1.3416172527964072 1101 48-49 1.598874105399009 1101 50-51 1.3587224397901565 1101 52-53 1.6173200903041085 1101 54-55 1.3955018975091988 1101 56-57 1.3892948597466734 1101 58-59 1.5586741363109908 1101 60-61 1.592514189871281 1101 62-63 1.4239069062978054 1101 64-65 1.4844038386195066 1101 66-67 1.5972216530805596 1101 68-69 1.650590394434392 1101 70-71 1.5028579133930116 1101 72-73 1.0559156640666494 1101 74-75 1.1819576015339734 1101 76-77 1.1515918146957205 1102 1 0.9408694995166371 1102 2 0.872739226613902 1102 3 0.908641084463099 1102 4 0.4937465866286246 1102 5 0.4961821464581462 1102 6 0.7982164563260525 1102 7 0.9210711111022931 1102 8 0.7067975709892664 1102 9 0.8392888680563715 1102 10-11 0.875807581073083 1102 12-13 1.2464182401814554 1102 14-15 1.2500287408334927 1102 16-17 0.9462250255743712 1102 18-19 1.057714770250918 1102 20-21 1.1629968880163872 1102 22-23 1.0271688000252084 1102 24-25 1.1553383388304859 1102 26-27 1.0265717704245567 1102 28-29 1.1057226132073978 1102 30-31 1.2660006645106883 1102 32-33 1.1448870872023953 1102 34-35 1.053239169244236 1102 36-37 1.038639617720694 1102 38-39 1.2017087236276218 1102 40-41 1.0937575322784916 1102 42-43 1.1755778481278831 1102 44-45 1.1731563740453588 1102 46-47 1.0717231294042797 1102 48-49 1.0217191434727297 1102 50-51 1.2005622135479967 1102 52-53 1.1797768247110518 1102 54-55 1.2297000955140156 1102 56-57 1.3753130299541887 1102 58-59 1.195288767996697 1102 60-61 1.3699653701834897 1102 62-63 1.3251110984319219 1102 64-65 0.9372812353321258 1102 66-67 1.0778722066118398 1102 68-69 1.1528589872360726 1102 70-71 1.0998573606666753 1102 72-73 0.8241288017813666 1102 74-75 0.647274023945684 1102 76-77 0.6659787982588199 1103 1 0.39074975241420873 1103 2 0.4517827044230245 1103 3 0.6979926837752686 1103 4 0.29257924719624384 1103 5 0.2700211710257818 1103 6 0.36319939041578664 1103 7 0.457187460607976 1103 8 0.02459495161880909 1103 9 0.20806318762787868 1103 10-11 0.5078917521166204 1103 12-13 0.45529770861860897 1103 14-15 0.3478083542139956 1103 16-17 0.46785223544090826 1103 18-19 0.2606445480563977 1103 20-21 0.1588018431047331 1103 22-23 0.47635940312460434 1103 24-25 0.4771528321262082 1103 26-27 0.371387578081098 1103 28-29 0.5170136480197769 1103 30-31 0.9151130813241153 1103 32-33 0.6779123509837 1103 34-35 0.8127851692754078 1103 36-37 0.645314544599632 1103 38-39 0.8819488931957515 1103 40-41 0.7254754486269555 1103 42-43 0.5570009424345734 1103 44-45 0.6174376057001574 1103 46-47 0.3790290014281368 1103 48-49 0.50535327321521 1103 50-51 0.4615459580736605 1103 52-53 0.43014048185509424 1103 54-55 0.22324258365619443 1103 56-57 0.1320160103842376 1103 58-59 -0.0732174843086284 1103 60-61 0.04047511359739886 1103 62-63 0.004058986363180139 1103 64-65 0.019567755600817804 1103 66-67 -0.023332277800260925 1103 68-69 -0.17024389136997442 1103 70-71 -0.3365922546360167 1103 72-73 -0.25493935276253055 1103 74-75 0.19029336531064445 1103 76-77 0.3361833294268344 1104 1 -0.1273694812280084 1104 2 -0.2854783134842265 1104 3 0.08836627309955958 1104 4 0.32792689421943777 1104 5 0.26268010547352105 1104 6 0.07844982951699819 1104 7 -0.11354316342044513 1104 8 0.08017406049571463 1104 9 0.1565949745174251 1104 10-11 0.25107558915455996 1104 12-13 0.631996828397341 1104 14-15 0.7036607657376237 1104 16-17 0.7816783715521822 1104 18-19 0.5477056992081586 1104 20-21 0.7274631396141231 1104 22-23 0.8108914322768896 1104 24-25 0.7632373616317913 1104 26-27 0.5411885188680472 1104 28-29 0.9387716174543002 1104 30-31 0.9334644834196872 1104 32-33 0.7891450140568033 1104 34-35 0.8643682048274641 1104 36-37 0.685600818396896 1104 38-39 0.7096433090397376 1104 40-41 0.40772642272503745 1104 42-43 0.45573133429454415 1104 44-45 0.3066347066227735 1104 46-47 0.27665911670482046 1104 48-49 0.3551289278293055 1104 50-51 0.48049923164884945 1104 52-53 0.3106317704909536 1104 54-55 0.3234951930277248 1104 56-57 0.10603964109507658 1104 58-59 -0.006191461253294506 1104 60-61 0.1998549714699287 1104 62-63 -0.19307221909580718 1104 64-65 -0.6132073640164073 1104 66-67 -0.4357833205394428 1104 68-69 -0.6349378832541781 1104 70-71 -0.5640630821651378 1104 72-73 -0.7132815185913692 1104 74-75 -0.9008617736875983 1104 76-77 -0.691685717471195 1105 1 0.09413947582228133 1105 2 0.4854448745436173 1105 3 0.10682451699612727 1105 4 0.03912721920088558 1105 5 -0.11208919782242788 1105 6 0.21174237299830878 1105 7 0.14251011748789466 1105 8 3.203156835240861E-5 1105 9 0.18267122479326048 1105 10-11 0.1694666300982064 1105 12-13 0.24220590982324808 1105 14-15 0.22232337682310543 1105 16-17 0.1270764738210417 1105 18-19 -0.03913656442802704 1105 20-21 -0.0947399136065954 1105 22-23 -0.17983807082247694 1105 24-25 -0.2262634307612359 1105 26-27 -0.30143119266076823 1105 28-29 -0.13791372227167642 1105 30-31 0.02125962474649512 1105 32-33 0.23851335645083438 1105 34-35 0.1969062196321758 1105 36-37 0.1615883005931309 1105 38-39 -0.1302820154401907 1105 40-41 -0.12976053789604336 1105 42-43 -0.224935529634525 1105 44-45 -0.32652744306929904 1105 46-47 -0.3579887699127653 1105 48-49 -0.28791682033013544 1105 50-51 -0.31518498468447476 1105 52-53 -0.3212439943187668 1105 54-55 -0.6957943112752716 1105 56-57 -0.8493368458956319 1105 58-59 -0.6352010702725295 1105 60-61 -0.3232610890327905 1105 62-63 -0.27212094625182104 1105 64-65 -0.2174070470591971 1105 66-67 -0.38741349366227595 1105 68-69 -0.5337134354217206 1105 70-71 -0.4730569937782114 1105 72-73 -0.6280578932428185 1105 74-75 -0.7098461565237635 1105 76-77 -0.7885244203539017 1106 1 -0.0659312412873021 1106 2 -0.19717790974722504 1106 3 -0.18128177355449537 1106 4 -0.10049511822423796 1106 5 -0.01906054713711569 1106 6 -0.2279124415524194 1106 7 -0.21937296974370923 1106 8 -0.06977425336592091 1106 9 0.08768906279345146 1106 10-11 0.025219354636014657 1106 12-13 0.6131789293901093 1106 14-15 0.5392634943756534 1106 16-17 0.5427177505383511 1106 18-19 0.5855438533303712 1106 20-21 0.3781136047893696 1106 22-23 0.5166778417481268 1106 24-25 0.36242842480590554 1106 26-27 0.2873851992526717 1106 28-29 0.37113469794814336 1106 30-31 0.08865942793022796 1106 32-33 0.13833729624361624 1106 34-35 0.09220289695361572 1106 36-37 0.4430465479516137 1106 38-39 0.399749558186425 1106 40-41 0.3794099767809307 1106 42-43 0.2723930436429072 1106 44-45 0.35044114691065786 1106 46-47 0.49294244451196434 1106 48-49 0.5823272953275023 1106 50-51 0.4432181663292738 1106 52-53 0.3401099355355228 1106 54-55 0.206266398972609 1106 56-57 0.043364103266679166 1106 58-59 0.09840958027115931 1106 60-61 -0.22315584361072283 1106 62-63 0.0010043544057829479 1106 64-65 -0.24687454429375677 1106 66-67 -0.6150793650870447 1106 68-69 -0.27104674596408884 1106 70-71 -0.2662546231922285 1106 72-73 -0.3576479733123641 1106 74-75 -0.2298672056081763 1106 76-77 -0.46859054671189426 1107 1 0.16889201159531098 1107 2 0.05529426219796818 1107 3 0.29565396679623746 1107 4 0.2723122239414053 1107 5 0.4655457108389882 1107 6 0.20229594084307223 1107 7 0.3415818527017507 1107 8 0.18545661351470955 1107 9 0.1944752280504929 1107 10-11 0.4879590302762935 1107 12-13 0.41248275141615665 1107 14-15 0.32638868754036565 1107 16-17 0.32528661137677517 1107 18-19 0.3393516373357741 1107 20-21 0.15622145406145904 1107 22-23 0.0856620364447025 1107 24-25 0.4941325581383431 1107 26-27 0.3273851543958415 1107 28-29 0.5390113775560046 1107 30-31 0.7981946439046439 1107 32-33 0.7806612836567908 1107 34-35 0.6529681992998526 1107 36-37 0.6184311391825545 1107 38-39 0.8058709452492714 1107 40-41 0.8647642223675795 1107 42-43 0.5635093730983662 1107 44-45 0.7548041463073325 1107 46-47 0.83991225362373 1107 48-49 0.6235462347775105 1107 50-51 0.6132804948920878 1107 52-53 0.663977356694037 1107 54-55 0.5861476629483136 1107 56-57 0.7018780414046226 1107 58-59 0.7387553815598942 1107 60-61 0.46076163776809054 1107 62-63 0.517824543567027 1107 64-65 0.6091361585454571 1107 66-67 0.7910364148722699 1107 68-69 0.5688547484411437 1107 70-71 0.4441373582011572 1107 72-73 0.3417608136917174 1107 74-75 0.456930084142261 1107 76-77 0.49359402567917243 1108 1 0.37149311279143404 1108 2 0.5127146786273116 1108 3 0.6049824903074068 1108 4 0.6547247294790282 1108 5 0.30298005043774623 1108 6 0.6201302551269734 1108 7 0.5327786124005058 1108 8 0.6541421397472291 1108 9 0.35320568814795195 1108 10-11 0.5615461450158605 1108 12-13 0.5422403616939917 1108 14-15 0.5246546200762765 1108 16-17 0.6028671442544393 1108 18-19 0.37053637247911553 1108 20-21 0.12092305522594415 1108 22-23 0.31917890246584335 1108 24-25 0.43099098482019826 1108 26-27 0.33640040659507164 1108 28-29 0.37867025990522407 1108 30-31 0.5869042192479768 1108 32-33 0.5275760358876589 1108 34-35 0.5111417326205299 1108 36-37 0.6012676685161402 1108 38-39 0.5344071138461963 1108 40-41 0.5083753862179705 1108 42-43 0.6768123286075145 1108 44-45 0.7768786985508598 1108 46-47 0.5351277463140676 1108 48-49 0.6938076106398015 1108 50-51 0.6869498829055019 1108 52-53 0.9003263219494038 1108 54-55 0.7661869009712206 1108 56-57 0.7906319177415675 1108 58-59 0.7104660327844989 1108 60-61 0.6969587139025073 1108 62-63 0.7279789641733174 1108 64-65 0.8788216214908395 1108 66-67 0.8428559199668868 1108 68-69 0.9706964040325623 1108 70-71 1.2176168848133955 1108 72-73 1.0707558140081517 1108 74-75 0.9161098828891774 1108 76-77 0.893970584124844 1109 1 -0.2516340560185242 1109 2 0.019736519819389287 1109 3 -0.26301097774729953 1109 4 -0.13102746484634764 1109 5 -0.04803035985006687 1109 6 -0.11646905085383708 1109 7 0.0619070059502107 1109 8 0.08359713056167806 1109 9 -0.02287106220926205 1109 10-11 -0.0015585702321416761 1109 12-13 -0.12472228374385708 1109 14-15 0.03741232573050013 1109 16-17 0.334771614554505 1109 18-19 0.176159969131497 1109 20-21 0.010829158512329684 1109 22-23 0.19571493022661457 1109 24-25 -0.08641052569215191 1109 26-27 -0.24464674588502788 1109 28-29 -0.0037649309500551453 1109 30-31 -0.3335795529504324 1109 32-33 -0.12072974080145826 1109 34-35 0.008937201083480062 1109 36-37 -0.018838475594900927 1109 38-39 -0.08268821173045282 1109 40-41 -0.13518452396778358 1109 42-43 0.05933937494350161 1109 44-45 -0.1884713728922911 1109 46-47 -0.25629907548630015 1109 48-49 0.01558756573267317 1109 50-51 0.02484537178078483 1109 52-53 -0.02998598668500918 1109 54-55 0.021228746245768093 1109 56-57 0.10282828434699454 1109 58-59 0.11718168904087634 1109 60-61 0.22552576840342553 1109 62-63 0.05287179944582121 1109 64-65 0.19806024161739444 1109 66-67 0.2365178917978703 1109 68-69 0.21919609784762528 1109 70-71 0.4336303569505695 1109 72-73 0.19300525266910284 1109 74-75 0.09574858466504921 1109 76-77 0.0733949564457248 1110 1 -0.21487407705762251 1110 2 -0.020167635402394524 1110 3 -0.22545229230128427 1110 4 -0.10061233228054789 1110 5 -0.2537401371862771 1110 6 -0.05404137275203169 1110 7 -0.25439439860436863 1110 8 -0.5092651607519016 1110 9 -0.2034663907503571 1110 10-11 -0.4106560319039758 1110 12-13 -0.3601410202291575 1110 14-15 -0.3331660546485935 1110 16-17 -0.5144550038001583 1110 18-19 -0.6340790583946543 1110 20-21 -0.9070852435292451 1110 22-23 -0.7151406597447547 1110 24-25 -0.6826478389214579 1110 26-27 -0.7035328425479932 1110 28-29 -0.5033460970615238 1110 30-31 -0.3248824185534538 1110 32-33 -0.32106188581538575 1110 34-35 -0.2769393305823087 1110 36-37 -0.3831090228062486 1110 38-39 -0.43993890816354764 1110 40-41 -0.3093707199820379 1110 42-43 -0.2032285638093505 1110 44-45 -0.24066294556483925 1110 46-47 -0.2971772630069971 1110 48-49 -0.41758213017444135 1110 50-51 -0.2159276202516338 1110 52-53 -0.30701927910898874 1110 54-55 -0.41077503299955254 1110 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-0.1286347955777245 2314 5 -0.3036726473097602 2314 6 -0.2749282397219872 2314 7 -0.2687055852597027 2314 8 -0.42972375862888157 2314 9 -0.1505309977259799 2314 10-11 -0.26270739086680095 2314 12-13 -0.20330704532539556 2314 14-15 -0.41521791353643067 2314 16-17 -0.5488615094909761 2314 18-19 -0.5018138981686917 2314 20-21 -0.6190367841314881 2314 22-23 -0.5056595819064285 2314 24-25 -0.34730480704290656 2314 26-27 -0.3115566389785265 2314 28-29 -0.5872368467823961 2314 30-31 -0.5947877660846608 2314 32-33 -0.6538453016363484 2314 34-35 -0.6857938357398687 2314 36-37 -1.2125004474258887 2314 38-39 -0.9004889923175909 2314 40-41 -0.7211339554967147 2314 42-43 -0.7338389722480834 2314 44-45 -0.8379283557533128 2314 46-47 -0.6406449095266886 2314 48-49 -0.45589554982815983 2314 50-51 -0.6319168885280604 2314 52-53 -0.372748586379565 2314 54-55 -0.3650685556378228 2314 56-57 -0.2785055015006215 2314 58-59 -0.28305588095742706 2314 60-61 -0.23740482721849787 2314 62-63 -0.33019285353228867 2314 64-65 -0.0270627857031549 2314 66-67 0.06875953442471783 2314 68-69 0.182194570611923 2314 70-71 0.1683240613456114 2314 72-73 0.09073136839962714 2314 74-75 0.2809343983422927 2314 76-77 0.4219695364559044 2315 1 -1.5248315536594603 2315 2 -1.2107146736940493 2315 3 -1.2895548564038535 2315 4 -1.1888578216771606 2315 5 -1.0469714006357194 2315 6 -1.1656215339908726 2315 7 -1.4102105629869897 2315 8 -1.4407764059427066 2315 9 -1.2454097683917666 2315 10-11 -1.5750111966606894 2315 12-13 -1.5098094818705832 2315 14-15 -1.5817520401171947 2315 16-17 -1.4461618772149691 2315 18-19 -1.4017242093887603 2315 20-21 -1.3916906751297233 2315 22-23 -1.460007992920211 2315 24-25 -1.6744177252168448 2315 26-27 -1.2546072533466734 2315 28-29 -1.0747641271196287 2315 30-31 -1.024202696276891 2315 32-33 -1.0997480192063769 2315 34-35 -1.1606675240414646 2315 36-37 -1.087438412686435 2315 38-39 -1.0418938175739534 2315 40-41 -1.000656512224868 2315 42-43 -0.9187877001622233 2315 44-45 -0.9347982173336291 2315 46-47 -0.7350901844227984 2315 48-49 -0.9022347527938699 2315 50-51 -0.910502197505906 2315 52-53 -1.0948673343241957 2315 54-55 -0.9849764751570902 2315 56-57 -1.2821662985349107 2315 58-59 -1.144552487731918 2315 60-61 -1.303240876128779 2315 62-63 -1.3933501975485534 2315 64-65 -1.4543984310139244 2315 66-67 -1.3417594646485007 2315 68-69 -1.2718324556071003 2315 70-71 -1.0968453009671641 2315 72-73 -0.6630863839995449 2315 74-75 -0.7865549137447658 2315 76-77 -0.5678537766476239 2316 1 -2.172603304539564 2316 2 -1.714602936947216 2316 3 -2.116652985842684 2316 4 -2.691753851776511 2316 5 -2.3762250154298385 2316 6 -2.4426691644090326 2316 7 -2.1005886441532766 2316 8 -1.7689313040250312 2316 9 -2.1544760366593465 2316 10-11 -2.102825992712411 2316 12-13 -2.5783340715055374 2316 14-15 -3.413799234920795 2316 16-17 -2.955811546309249 2316 18-19 -2.4220561154121754 2316 20-21 -2.3109148294728 2316 22-23 -2.6467244917408266 2316 24-25 -2.579577982971589 2316 26-27 -2.4029646637956112 2316 28-29 -2.986823228721075 2316 30-31 -2.958117548151808 2316 32-33 -3.1328902199311166 2316 34-35 -2.87609182875223 2316 36-37 -2.7528775605598277 2316 38-39 -2.910610884306873 2316 40-41 -2.8541262628274673 2316 42-43 -2.9218910505221665 2316 44-45 -2.650493571101048 2316 46-47 -2.846350175323291 2316 48-49 -2.4213654920156387 2316 50-51 -2.596206295832175 2316 52-53 -2.4393497112953533 2316 54-55 -2.5126299324846144 2316 56-57 -2.5130355593131455 2316 58-59 -2.4496327214794604 2316 60-61 -2.7490213844748332 2316 62-63 -2.8133253836527707 2316 64-65 -2.5907756344975503 2316 66-67 -2.4376382282775886 2316 68-69 -2.4412445695638674 2316 70-71 -2.167708495409368 2316 72-73 -2.0010926245813963 2316 74-75 -2.2139008594639655 2316 76-77 -2.687244432554074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16368.0 3 2698.0 4 5238.0 5 6716.0 6 6878.0 7 6767.0 8 8930.0 9 10303.0 10 15380.0 11 17778.0 12 10712.0 13 6354.0 14 5483.0 15 5320.0 16 5160.0 17 5283.0 18 5537.0 19 5719.0 20 5840.0 21 6244.0 22 6866.0 23 7272.0 24 8499.0 25 9531.0 26 10960.0 27 12184.0 28 13915.0 29 15854.0 30 18345.0 31 21790.0 32 25992.0 33 32574.0 34 49099.0 35 60595.0 36 67277.0 37 79595.0 38 66976.0 39 17583.0 40 168.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.487876718238816 25.827803915661327 38.110350455637665 17.57396891046219 2 18.520788207023543 26.41280390330087 37.75750333831094 17.30890455136465 3 18.180767756901357 26.554103664440564 37.641471626693345 17.623656951964733 4 18.431453429522623 26.531773475393916 37.63639980574912 17.40037328933434 5 18.015673816126654 26.689367079720572 37.66220849276279 17.63275061138999 6 18.18240323887587 26.823403452421697 37.76089888166344 17.233294427039 7 18.177882182235187 26.90465812253352 37.14928049661677 17.76817919861453 8 17.507902354543287 27.352751452848434 36.93372553780624 18.205620654802043 9 17.737306778689195 27.315584081255338 37.13593653096807 17.811172609087397 10-11 18.041420711233265 27.31562576108747 36.987845273157724 17.655108254521544 12-13 18.265513443302044 27.34019942982437 36.111440227368135 18.282846899505447 14-15 18.132682193399678 27.69962733500652 36.074863541428996 18.092826930164804 16-17 18.058114978032844 27.97536519302433 35.78313940223591 18.183380426706915 18-19 18.15068392969975 28.26109849991809 34.97651002784863 18.611707542533523 20-21 18.270066848664307 28.43792347177196 34.51017502342007 18.781834656143666 22-23 18.71058097355488 28.93415047975661 33.085654107184645 19.26961443950386 24-25 18.72803020440047 29.669664969302627 31.589402714124592 20.01290211217231 26-27 19.117046935494958 30.114175721789195 30.88675599656566 19.88202134615019 28-29 19.18255804004648 30.343241964487472 30.420026604049404 20.054173391416647 30-31 18.952642890729607 30.266822966066307 30.388727722399395 20.391806420804688 32-33 19.280246826005655 30.620845798831542 29.749802733719406 20.349104641443397 34-35 19.20088985030945 30.077788771709663 30.24540357358853 20.475917804392356 36-37 19.38137273327595 30.334338369238438 30.473137167290577 19.81115173019503 38-39 19.816431233018974 30.23860282959187 30.51768586337657 19.427280074012586 40-41 19.66745794592096 30.567000583586733 30.478365606118444 19.28717586437386 42-43 19.587251137037626 30.719432720794458 30.17071470831941 19.522601433848507 44-45 19.793258900296493 30.574981313728962 30.18831080497969 19.443448980994855 46-47 19.36809972091717 30.90462037129133 30.139190367254283 19.588089540537222 48-49 19.341436480250408 30.902681790590698 29.851978615883045 19.90390311327585 50-51 18.83256141911285 31.325722444417785 29.851649107202938 19.990067029266424 52-53 18.3678783764805 31.320660315528297 30.272386007209402 20.039075300781796 54-55 18.26024689985474 31.112921794566095 30.274489209285587 20.352342096293576 56-57 18.25811717280458 31.24917723944453 30.098482610132898 20.394222977617986 58-59 18.35384529872774 30.95115158571583 29.627698320524935 21.06730479503149 60-61 18.429151728183232 30.538648434768746 29.662818133015033 21.36938170403299 62-63 18.39763956910726 30.488630844758074 29.568214931451543 21.54551465468312 64-65 18.40109180030762 30.130485056860945 29.745853638200725 21.72256950463072 66-67 18.423978152211912 30.232622773130263 29.686940037066346 21.65645903759148 68-69 18.594755991052736 29.85856419544123 29.833328700730437 21.713351112775598 70-71 18.551667725121675 29.549990781198325 30.07187738701115 21.82646410666885 72-73 18.881944764576623 29.21142114540733 29.997007221450247 21.9096268685658 74-75 18.95067158248163 28.98357378186486 29.91041176048721 22.155342875166305 76-77 19.14755043691626 28.602226549550718 30.61933730403532 21.630885709497704 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 40328.0 1 23156.5 2 5366.0 3 4519.5 4 4292.0 5 4277.0 6 4123.5 7 4021.0 8 3981.5 9 3906.0 10 3921.5 11 3917.5 12 4010.0 13 4122.0 14 4366.5 15 4758.5 16 4994.5 17 5083.0 18 5346.5 19 5816.0 20 6314.0 21 7073.5 22 7541.0 23 7841.0 24 8574.5 25 9553.0 26 10098.0 27 10416.5 28 11467.5 29 12489.0 30 12778.0 31 13632.0 32 15141.0 33 16444.5 34 17339.0 35 17585.0 36 18100.0 37 19182.5 38 19676.0 39 19602.0 40 19778.0 41 20313.0 42 21219.0 43 21766.0 44 21501.0 45 21642.5 46 22011.0 47 22335.5 48 22698.0 49 22769.5 50 23021.5 51 22243.5 52 21285.0 53 21225.5 54 20324.5 55 18722.5 56 17414.5 57 16867.0 58 17144.0 59 15886.5 60 13660.0 61 12968.0 62 11871.5 63 8459.5 64 5267.0 65 4390.0 66 3957.5 67 3158.0 68 2426.0 69 1793.5 70 1526.0 71 1307.5 72 1015.5 73 923.0 74 904.0 75 818.5 76 529.5 77 279.5 78 233.0 79 208.5 80 148.0 81 99.0 82 78.0 83 70.0 84 52.5 85 42.0 86 36.0 87 23.0 88 18.0 89 17.0 90 15.5 91 10.0 92 11.0 93 8.0 94 4.0 95 5.0 96 6.0 97 5.5 98 6.5 99 15.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002339923630742502 2 0.007897242253755942 3 0.01126088247294829 4 0.01915812472670423 5 0.013893296557533602 6 0.013015825196005165 7 0.017110691549804543 8 0.018426898592097198 9 0.016233220188276106 10-11 0.020401209155536188 12-13 0.019596860407468452 14-15 0.008628468388362974 16-17 0.005118582942249222 18-19 0.012723334742162353 20-21 0.010895269405644772 22-23 0.015063258372904854 24-25 0.025446669484324707 26-27 0.014624522692140634 28-29 0.007677874413373834 30-31 0.007677874413373834 32-33 0.009140326682587897 34-35 0.01060277895180196 36-37 0.008701591001823678 38-39 0.016891323709422434 40-41 0.011699618153712508 42-43 0.014405154851758526 44-45 0.018426898592097198 46-47 0.01681820109596173 48-49 0.014332032238297822 50-51 0.029395290611202676 52-53 0.03378264741884487 54-55 0.027201612207381584 56-57 0.015940729734433293 58-59 0.017110691549804543 60-61 0.02135180313052533 62-63 0.02617789561893174 64-65 0.020693699609378998 66-67 0.021059312676682512 68-69 0.03246644037655221 70-71 0.058059355087798324 72-73 0.0689546244934431 74-75 0.07984989389908786 76-77 0.07729060242796325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 683783.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.60947729958187 #Duplication Level Percentage of deduplicated Percentage of total 1 81.6722247914059 46.2342195533544 2 9.419267538206523 10.66439623765581 3 3.613911662242501 6.137449506192331 4 1.740581284057019 3.9413358675161145 5 0.9712040140894039 2.748967579442845 6 0.5940507606892139 2.017734183122124 7 0.40337869069942783 1.598453978399902 8 0.26423111335006744 1.196638817042711 9 0.2005802482663263 1.0219268709879386 >10 1.0243018342960752 10.25754899382176 >50 0.05952061271156565 2.2835943705337773 >100 0.03493595597263503 3.7854761761260693 >500 0.0010351394362208258 0.43641383624796226 >1k 2.5878485905520644E-4 0.17726107481035056 >5k 0.0 0.0 >10k+ 5.175697181104129E-4 7.498582954745954 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30864 4.513712683702285 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20322 2.97199550149682 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 1210 0.1769567245749017 No Hit GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 870 0.12723334742162354 No Hit AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG 760 0.11114637246026882 No Hit TAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC 713 0.10427284679496274 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.312261346070318E-4 0.0 2 0.0 0.0 0.0 0.004094866353799378 0.0 3 0.0 0.0 0.0 0.005264828169170629 0.0 4 0.0 0.0 0.0 0.009359694522970006 0.0 5 0.0 0.0 0.0 0.01754942723056876 0.0 6 0.0 0.0 0.0 0.03290517605731643 0.0 7 0.0 0.0 0.0 0.05454946964168457 0.0 8 0.0 0.0 0.0 0.07634000845297412 0.0 9 0.0 0.0 0.0 0.09681434022197101 0.0 10 0.0 0.0 0.0 0.12109104789092447 0.0 11 0.0 0.0 0.0 0.1541424691751623 0.0 12 0.0 0.0 0.0 0.18792511659400715 0.0 13 0.0 0.0 0.0 0.2395496816972636 0.0 14 0.0 0.0 0.0 0.29292918952357694 0.0 15 0.0 0.0 0.0 0.363858124580459 0.0 16 0.0 0.0 0.0 0.4375657189488478 0.0 17 0.0 0.0 0.0 0.502791090155795 0.0 18 0.0 0.0 0.0 0.5642140854627857 0.0 19 0.0 0.0 0.0 0.6273920234928333 0.0 20 0.0 0.0 0.0 0.6945185826497587 0.0 21 0.0 0.0 0.0 0.7677874413373833 0.0 22 0.0 0.0 0.0 0.847929825690314 0.0 23 0.0 0.0 0.0 0.9273409839086377 0.0 24 0.0 0.0 0.0 1.0118707250692105 0.0 25 0.0 0.0 0.0 1.1053214250719892 0.0 26 0.0 0.0 0.0 1.2003808225709034 0.0 27 0.0 0.0 0.0 1.2996813316505382 0.0 28 0.0 0.0 0.0 1.3926932959725526 0.0 29 0.0 0.0 0.0 1.479855451217711 0.0 30 0.0 0.0 0.0 1.5674563421436334 0.0 31 0.0 0.0 0.0 1.6657331346348183 0.0 32 0.0 0.0 0.0 1.7676660577990386 0.0 33 0.0 0.0 0.0 1.876911242309329 0.0 34 0.0 0.0 0.0 1.9949311404349042 0.0 35 0.0 0.0 0.0 2.1179233762758067 0.0 36 0.0 0.0 0.0 2.263729867516449 0.0 37 0.0 0.0 0.0 2.4156786582877903 0.0 38 0.0 0.0 0.0 2.5525641906862266 0.0 39 0.0 0.0 0.0 2.679066311973243 0.0 40 0.0 0.0 0.0 2.8159518443716793 0.0 41 0.0 0.0 0.0 2.956639752670072 0.0 42 0.0 0.0 0.0 3.108442298214492 0.0 43 0.0 0.0 0.0 3.2716519714587817 0.0 44 0.0 0.0 0.0 3.4524110719336396 0.0 45 0.0 1.4624522692140634E-4 0.0 3.645016035789132 0.0 46 0.0 1.4624522692140634E-4 0.0 3.8646763666250843 0.0 47 0.0 1.4624522692140634E-4 0.0 4.069858419995818 0.0 48 0.0 1.4624522692140634E-4 0.0 4.287910053335635 0.0 49 0.0 1.4624522692140634E-4 0.0 4.493238351933289 0.0 50 0.0 1.4624522692140634E-4 0.0 4.695056765084829 0.0 51 0.0 1.4624522692140634E-4 0.0 4.89892261141327 0.0 52 0.0 1.4624522692140634E-4 0.0 5.108492021591645 0.0 53 0.0 1.4624522692140634E-4 0.0 5.339120744446703 0.0 54 0.0 1.4624522692140634E-4 0.0 5.589638818163072 0.0 55 0.0 1.4624522692140634E-4 0.0 5.8477616436793545 0.0 56 0.0 1.4624522692140634E-4 0.0 6.129722441183826 0.0 57 0.0 1.4624522692140634E-4 0.0 6.411829483915219 0.0 58 0.0 1.4624522692140634E-4 0.0 6.708707294565674 0.0 59 0.0 1.4624522692140634E-4 0.0 7.005438859989207 0.0 60 0.0 1.4624522692140634E-4 0.0 7.297490578151256 0.0 61 0.0 1.4624522692140634E-4 0.0 7.591589729490204 0.0 62 0.0 1.4624522692140634E-4 0.0 7.897242253755943 0.0 63 0.0 1.4624522692140634E-4 0.0 8.202163551887075 0.0 64 0.0 1.4624522692140634E-4 0.0 8.520539410894976 0.0 65 0.0 1.4624522692140634E-4 0.0 8.859535846898797 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAATCG 40 0.0022501769 35.51922 71 CGATCCG 60 1.1024538E-5 35.503624 46 TTCGCGA 50 0.0067275055 28.398743 25 TGACACG 50 0.0067347204 28.39251 2 CGATTAT 65 7.312624E-4 27.314478 60 CGGTATA 65 7.3282776E-4 27.304485 10 GTGGGTA 180 9.094947E-12 25.632126 1 GCGCTCC 105 1.8288036E-5 23.663887 9 GGCCGAC 120 1.9222334E-6 23.656965 28 TAGCTTA 80 0.0024579915 22.183271 4 GATCCGA 100 3.569815E-4 21.302174 47 ATGGCGG 85 0.003494093 20.878372 6 TATATAG 120 5.1211508E-5 20.708931 13 TCAGCGC 105 4.9716537E-4 20.281849 6 ATTTGCG 385 0.0 20.281847 3 ATCGATT 90 0.004854435 19.725677 58 GAGTGGT 95 0.0066457484 18.682016 14 GTGCTAC 95 0.0066457484 18.682016 16 ACGTAGG 95 0.006648556 18.680649 3 TAGTATG 95 0.006648556 18.680649 5 >>END_MODULE