FastQCFastQC Report
Thu 26 May 2016
SRR936170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683783
Sequences flagged as poor quality0
Sequence length125
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173682.539987101170986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123861.8113933806485392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101331.4819028843946105No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47840.699637165592008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33920.4960638097174104No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29690.43420207872965544No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23390.34206758576916946No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21860.3196920660501943No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21090.308431183577246No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16910.24730067872409814No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11420.16701204914424606No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.15955354257125431No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10310.15077882895596995No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT9830.14375905806374245No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT8070.11801989812557494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7750.11334005086408992No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC6960.10178667793729883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6860.10032422566808474No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG6840.10003173521424195No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGT150.004230612459.52169498-99
TGCGACG201.768911E-459.52169498-99
GACGATA150.00423183859.51733896-97
TACGCAG6500.056.77869100-101
CGTATCA10250.055.75123100-101
CGAGTAC5700.051.171516100-101
AGGTATC15700.050.616093100-101
CCGTACA3350.049.753117100-101
AGTATCA22500.049.73732100-101
CGTACAT9200.049.17369100-101
ATACGCA1150.049.17369100-101
CTACGCA2250.048.943638100-101
CGGTATC9250.048.907887100-101
TCGAGTA2100.048.187756100-101
CCCGGTA2100.048.1842398-99
TTTACGC2600.048.0752298-99
AAGTATC16450.047.946514100-101
GCGTACC255.321464E-447.62084100-101
ATATCAA15450.047.38967100-101
CCGTATC3950.046.716644100-101
Produced by FastQC (version 0.11.5)