FastQCFastQC Report
Thu 26 May 2016
SRR936169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947430
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59760.15138963832164218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA500.006729657828.40019820
CGCGGAA4500.023.6662359
CGATCGT5400.022.347761
CTCGCGC5700.021.17129336
ATTCGCG4950.020.79812256
TCGCGCT5700.019.92693337
GACGTAT5350.019.90466339
GCGTAAG7500.019.87686338
TTGCGTA7550.019.74447836
TGCGTAA7550.019.27534937
TCGCGGA5550.019.18810758
CCTCGCG6050.018.7736635
CGCGCTT6100.018.62001438
CGGTTAC3850.018.437484
CGTAAGT7900.018.4223139
CGTATTA6200.018.32108141
CAATTCG6550.017.88516654
AATTCGC6000.017.74922455
ACGTATT6200.017.17623340
CCGAACG1502.8384727E-416.56594355
Produced by FastQC (version 0.11.5)