##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936169_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3947430 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.27238203084032 39.0 36.0 41.0 29.0 41.0 2 36.135854214007594 39.0 36.0 41.0 28.0 41.0 3 36.043738837674134 39.0 36.0 41.0 28.0 41.0 4 35.91624575989948 39.0 35.0 41.0 27.0 41.0 5 35.84763859017133 39.0 35.0 41.0 27.0 41.0 6 35.746825149527666 39.0 35.0 40.0 27.0 41.0 7 35.58644383814279 39.0 35.0 40.0 27.0 41.0 8 35.405430621948966 38.0 35.0 40.0 27.0 41.0 9 35.317576245810564 38.0 34.0 40.0 26.0 41.0 10-11 35.534335504366126 39.0 35.0 40.0 27.0 41.0 12-13 35.725808690717756 39.0 35.0 41.0 27.0 41.0 14-15 35.67317976506233 39.0 35.0 41.0 27.0 41.0 16-17 35.50719214780249 39.0 35.0 41.0 26.5 41.0 18-19 35.358251824604864 39.0 35.0 41.0 26.0 41.0 20-21 35.17062607823318 39.0 35.0 41.0 25.0 41.0 22-23 34.92335532232364 39.0 34.0 41.0 24.0 41.0 24-25 34.696045275027046 39.0 34.0 41.0 23.5 41.0 26-27 33.76526258857028 38.0 33.0 39.5 22.5 40.5 28-29 33.7968124830586 38.0 33.0 39.5 22.0 40.5 30-31 34.16591693836243 38.0 33.0 40.0 22.0 41.0 32-33 34.00649333870392 38.0 33.0 40.0 21.5 41.0 34-35 33.73244617383969 38.0 33.0 40.0 20.0 41.0 36-37 33.436003678342615 37.5 33.0 40.0 18.5 41.0 38-39 33.14695422084749 37.0 32.5 40.0 18.0 41.0 40-41 32.76008390269112 36.5 32.0 40.0 14.0 41.0 42-43 32.404254666960526 36.0 32.0 39.0 12.5 41.0 44-45 32.03339489237301 35.5 31.0 39.0 8.0 40.5 46-47 31.637818910025004 35.0 31.0 38.5 7.5 40.0 48-49 31.170896000689055 35.0 31.0 37.5 4.5 40.0 50-51 30.732999445208655 35.0 30.0 37.0 2.0 39.0 52-53 30.28065475511915 35.0 30.0 36.5 2.0 39.0 54-55 29.82307564668658 34.0 29.5 36.0 2.0 38.5 56-57 29.37340991480533 34.0 29.0 35.5 2.0 37.0 58-59 28.95278016329612 34.0 29.0 35.0 2.0 37.0 60-61 28.605312443792542 34.0 29.0 35.0 2.0 36.5 62-63 28.2589308740117 34.0 28.5 35.0 2.0 36.0 64-65 27.892038237536827 34.0 27.0 35.0 2.0 36.0 66-67 27.514507160354967 34.0 26.5 35.0 2.0 35.0 68-69 27.152726457467264 33.0 26.0 35.0 2.0 35.0 70-71 26.732567011954615 33.0 25.0 35.0 2.0 35.0 72-73 26.24881429183038 33.0 24.5 35.0 2.0 35.0 74-75 25.664514633571716 33.0 23.5 35.0 2.0 35.0 76-77 23.97414722996988 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.838988428134293 1101 2 0.6249849343430185 1101 3 0.8659491886971225 1101 4 0.8094965685392665 1101 5 0.9613734631243105 1101 6 0.8966613562123769 1101 7 0.9887107615305339 1101 8 1.0800417883495683 1101 9 1.1259502971504247 1101 10-11 0.9213599370995738 1101 12-13 1.0157271629085685 1101 14-15 1.1605014245627387 1101 16-17 1.2833246212430538 1101 18-19 1.277463190859315 1101 20-21 1.0955699373994818 1101 22-23 1.1392350923896117 1101 24-25 1.0758984374701086 1101 26-27 0.9619980598525757 1101 28-29 1.1151505307062308 1101 30-31 1.1849481215004047 1101 32-33 1.2854188424188848 1101 34-35 1.1695082546504878 1101 36-37 1.285077584700275 1101 38-39 1.2774283010461858 1101 40-41 1.1421945116411152 1101 42-43 1.1487173070963266 1101 44-45 1.038848312027099 1101 46-47 1.0552155830330427 1101 48-49 1.133720345898734 1101 50-51 1.0442350618531862 1101 52-53 1.1430917530165203 1101 54-55 1.2580687064571272 1101 56-57 1.1017161338941008 1101 58-59 1.1583233742957653 1101 60-61 1.0517350097062277 1101 62-63 1.0574539969049042 1101 64-65 0.9701226276164441 1101 66-67 1.0806505684038292 1101 68-69 1.1662034445758032 1101 70-71 1.192546305584969 1101 72-73 1.2331525183617913 1101 74-75 1.2214627620533527 1101 76-77 1.1496751739141224 1102 1 0.9843340145774988 1102 2 1.0819540100551919 1102 3 1.0182265754012576 1102 4 1.032590144258613 1102 5 1.1142164331175763 1102 6 0.9793540774636895 1102 7 1.031207643295673 1102 8 1.1048679557163439 1102 9 1.088311410252743 1102 10-11 0.8700832528943252 1102 12-13 0.9960249364171929 1102 14-15 0.9107182673320224 1102 16-17 0.8732408948448622 1102 18-19 0.917919600086428 1102 20-21 0.8799318103475358 1102 22-23 0.9536972506382426 1102 24-25 1.0478117433725416 1102 26-27 0.8646216781519556 1102 28-29 0.9061682222088621 1102 30-31 1.0674623506870802 1102 32-33 1.0593923338383675 1102 34-35 1.1921176079214106 1102 36-37 1.1411792893353336 1102 38-39 1.187141423438696 1102 40-41 1.1175006004427033 1102 42-43 1.2034218991451837 1102 44-45 1.1760019012229534 1102 46-47 1.124152587520431 1102 48-49 1.2153080650712234 1102 50-51 1.2801574859044536 1102 52-53 1.2835628573734468 1102 54-55 1.2227416143724277 1102 56-57 1.2896528659839674 1102 58-59 1.2079380751602677 1102 60-61 1.1405219088187835 1102 62-63 1.2162517202350358 1102 64-65 1.1075500480118485 1102 66-67 1.1111655430995313 1102 68-69 0.9679234916002137 1102 70-71 1.1127069484431402 1102 72-73 1.1562192127461017 1102 74-75 1.0367772378314761 1102 76-77 1.273575906520108 1103 1 0.34767535832149576 1103 2 0.2058166797274339 1103 3 0.3234229354859224 1103 4 0.39571724054680857 1103 5 0.41521735254850256 1103 6 0.30381409081118704 1103 7 0.3712965502612917 1103 8 0.47584788004230916 1103 9 0.3702170955438575 1103 10-11 0.35202144765901977 1103 12-13 0.3524774224610283 1103 14-15 0.5725325967059831 1103 16-17 0.5407468883315119 1103 18-19 0.5460284796123318 1103 20-21 0.5106222571451369 1103 22-23 0.5680340716986549 1103 24-25 0.5147796119827106 1103 26-27 0.4878215555611618 1103 28-29 0.4354939871839889 1103 30-31 0.7111777109120965 1103 32-33 0.6590568561209622 1103 34-35 0.7479023608350914 1103 36-37 0.6629372928240187 1103 38-39 0.5267938155344538 1103 40-41 0.5599122162065058 1103 42-43 0.591576993680448 1103 44-45 0.5704717103765944 1103 46-47 0.3590800068983633 1103 48-49 0.43424397414780813 1103 50-51 0.48980012515814053 1103 52-53 0.5025306314626832 1103 54-55 0.5253559775447805 1103 56-57 0.6057207807088929 1103 58-59 0.4743179089752765 1103 60-61 0.5274246725536784 1103 62-63 0.4045513172321371 1103 64-65 0.4733072609617892 1103 66-67 0.48810916565738083 1103 68-69 0.4344340793772261 1103 70-71 0.45035571150778964 1103 72-73 0.45889107615475666 1103 74-75 0.31849287875503407 1103 76-77 0.30630492645256524 1104 1 0.7662811785972679 1104 2 0.8425391871544079 1104 3 0.7630717472052666 1104 4 0.6895968793665475 1104 5 0.7042047383537451 1104 6 0.4712900705770835 1104 7 0.7450015181277223 1104 8 0.8477924820146967 1104 9 0.7533221918056014 1104 10-11 0.7475602137848298 1104 12-13 0.8810632134075362 1104 14-15 0.856599296471245 1104 16-17 0.8047432755646469 1104 18-19 0.8313532521233142 1104 20-21 0.7933361546832955 1104 22-23 0.794441821988336 1104 24-25 0.8401801666606943 1104 26-27 0.6905836999019499 1104 28-29 0.5923570388431756 1104 30-31 0.7399349154224097 1104 32-33 0.7614066719586248 1104 34-35 0.8550065897832013 1104 36-37 0.7890240406375426 1104 38-39 0.8538215404996023 1104 40-41 0.9442941751185927 1104 42-43 0.9515362954560942 1104 44-45 0.83659408668548 1104 46-47 0.7604776070942982 1104 48-49 0.8581574461407122 1104 50-51 0.7992507806705547 1104 52-53 0.8438333660390427 1104 54-55 0.7779840825056112 1104 56-57 0.784520443313113 1104 58-59 0.7777904677022356 1104 60-61 0.6562878592165617 1104 62-63 0.7111729221622696 1104 64-65 0.6542897432400743 1104 66-67 0.728795187202099 1104 68-69 0.6368772562239791 1104 70-71 0.47911865934744213 1104 72-73 0.32235462423613725 1104 74-75 0.3501754586982777 1104 76-77 0.3837040533814218 1105 1 0.04172292738452654 1105 2 0.09148281001893821 1105 3 -0.016630217855336582 1105 4 0.11031804011649626 1105 5 -0.006711691599186054 1105 6 -0.26805115003563174 1105 7 -0.08885376339335238 1105 8 0.12540973945603184 1105 9 -0.11767772461537618 1105 10-11 0.08675610366021402 1105 12-13 0.14751818394732652 1105 14-15 -0.017059337353913406 1105 16-17 -0.004885238119875623 1105 18-19 0.05837210620443756 1105 20-21 0.05962228437999073 1105 22-23 0.051789904494853545 1105 24-25 -0.009907570277427169 1105 26-27 -0.24426869617959568 1105 28-29 -0.028554881208158633 1105 30-31 -0.04119732369885298 1105 32-33 0.13503217567648562 1105 34-35 0.1790610389918328 1105 36-37 -0.0035416614486791786 1105 38-39 -0.01931307669725868 1105 40-41 0.06538464716617653 1105 42-43 -0.005589419172579824 1105 44-45 -0.15548687109645343 1105 46-47 -0.2971434674963369 1105 48-49 -0.21677353104950825 1105 50-51 -0.1710696565318699 1105 52-53 -0.22987835308607885 1105 54-55 -0.263824114577087 1105 56-57 -0.0718899204295198 1105 58-59 -0.1610962989900102 1105 60-61 -0.2253863323667531 1105 62-63 -0.2387705708462633 1105 64-65 -0.19433676314558923 1105 66-67 -0.16334317506415985 1105 68-69 -0.3472786293097805 1105 70-71 -0.7927969942741662 1105 72-73 -0.7668635276690168 1105 74-75 -0.8769031698092355 1105 76-77 -0.6053332467020418 1106 1 0.19196607387912934 1106 2 0.24027967236757064 1106 3 -0.23309548046090356 1106 4 0.016336428273348247 1106 5 -0.16438698437578125 1106 6 0.1631573054293014 1106 7 0.2541751795350038 1106 8 0.2607849888080551 1106 9 0.14994067801632838 1106 10-11 -0.05838843711364916 1106 12-13 0.046000246433472114 1106 14-15 0.0987796968678083 1106 16-17 0.23369496219856956 1106 18-19 0.21838175233941826 1106 20-21 0.09621653152911591 1106 22-23 0.14976362245226937 1106 24-25 0.24945723088553962 1106 26-27 0.2712937537803768 1106 28-29 0.21114478813183268 1106 30-31 0.24389540443845448 1106 32-33 0.17901997891342347 1106 34-35 0.24555583087053634 1106 36-37 0.3070804237901328 1106 38-39 0.23954627066763834 1106 40-41 0.3134237917586873 1106 42-43 0.3879651843972738 1106 44-45 -0.043291394376090864 1106 46-47 0.157110825768779 1106 48-49 0.09790094864724352 1106 50-51 0.14942448595726177 1106 52-53 0.10709369630484389 1106 54-55 0.22633407836144315 1106 56-57 0.1695842264020655 1106 58-59 0.04798183922677879 1106 60-61 0.07234005495779883 1106 62-63 -0.07948597509056299 1106 64-65 -0.1500632440478249 1106 66-67 -0.22661812411944737 1106 68-69 -0.11096867739344773 1106 70-71 -0.3962763879586042 1106 72-73 -0.3336721299180496 1106 74-75 -0.4783452344779171 1106 76-77 -0.47355384719373816 1107 1 0.2794409595454681 1107 2 0.3478148096950946 1107 3 0.3315828565800203 1107 4 0.3839583853794437 1107 5 0.2294725390808381 1107 6 0.1586732637678807 1107 7 0.15068293570120517 1107 8 0.3070888018042339 1107 9 0.11789376498887805 1107 10-11 0.22931768957442955 1107 12-13 0.4203451651776646 1107 14-15 0.3436274728422717 1107 16-17 0.3529223864824331 1107 18-19 0.38740114032592743 1107 20-21 0.3102076056174141 1107 22-23 0.348237196043911 1107 24-25 0.14934729527374202 1107 26-27 0.1853562715456789 1107 28-29 0.39382550538830685 1107 30-31 0.5661193080126452 1107 32-33 0.5235418137838579 1107 34-35 0.5903689392968587 1107 36-37 0.6259454822712058 1107 38-39 0.421548417698979 1107 40-41 0.3945106080229479 1107 42-43 0.49445988183350664 1107 44-45 0.5033585772924312 1107 46-47 0.4541024598713186 1107 48-49 0.5127351656206152 1107 50-51 0.3918862581118816 1107 52-53 0.3441440647173124 1107 54-55 0.4213623963941302 1107 56-57 0.31197492698215257 1107 58-59 0.3500077098587333 1107 60-61 0.44285324836819484 1107 62-63 0.46000236745540946 1107 64-65 0.46521513925783964 1107 66-67 0.4130419643954646 1107 68-69 0.30459527389668395 1107 70-71 0.31589551842785113 1107 72-73 0.38812358705832395 1107 74-75 0.4054541882257503 1107 76-77 0.452401479422317 1108 1 0.028510647461992278 1108 2 0.1415442188172804 1108 3 0.1704334635818583 1108 4 0.0431784068294121 1108 5 0.07720736225684277 1108 6 0.03730707155692414 1108 7 0.031613656713069815 1108 8 0.15008321647460576 1108 9 -0.03525762089313389 1108 10-11 -0.0529319934072916 1108 12-13 0.009527507926428314 1108 14-15 0.23217700384881823 1108 16-17 0.03495152470437546 1108 18-19 -0.010304435580565041 1108 20-21 -0.029664433380524713 1108 22-23 -0.03289196388877258 1108 24-25 0.013122383869955456 1108 26-27 -0.26864066761545047 1108 28-29 -0.055275948559533106 1108 30-31 -0.06764778573724328 1108 32-33 0.00876043539491178 1108 34-35 -0.022489566937125005 1108 36-37 0.022343376309756025 1108 38-39 -0.08478868824567343 1108 40-41 -0.035485889416499106 1108 42-43 0.010415299551880253 1108 44-45 -0.07501817850678805 1108 46-47 -0.136075929705477 1108 48-49 -0.08260530282167267 1108 50-51 -0.04749346666548249 1108 52-53 -0.13249792598559296 1108 54-55 -0.007030884458085751 1108 56-57 -0.029685926777350602 1108 58-59 -0.08001466662365786 1108 60-61 -0.23734855285270484 1108 62-63 -0.14904777091845034 1108 64-65 -0.21779108099731204 1108 66-67 -0.2442010759693254 1108 68-69 -0.11666576247798943 1108 70-71 -0.11613633610584984 1108 72-73 0.01754329792026965 1108 74-75 0.0571741271986177 1108 76-77 0.15547087219922062 1109 1 0.16425217601864972 1109 2 0.08270658334365777 1109 3 0.08736225888539906 1109 4 0.42059275472139035 1109 5 0.17330541465157978 1109 6 0.1987648508545874 1109 7 -0.03583856850428191 1109 8 0.3181179818760427 1109 9 0.3261435696121353 1109 10-11 0.19285017822077322 1109 12-13 0.2321613964314011 1109 14-15 0.2973936171836016 1109 16-17 0.38258252401596593 1109 18-19 0.2182271830952871 1109 20-21 0.19945933861378506 1109 22-23 0.18132120631544524 1109 24-25 0.17840069461636432 1109 26-27 0.12011080948466457 1109 28-29 0.2685374925459598 1109 30-31 0.28509990940872143 1109 32-33 0.265227599318024 1109 34-35 0.33392852009641416 1109 36-37 0.3533664055449819 1109 38-39 0.38055317162266533 1109 40-41 0.3753123001623848 1109 42-43 0.33089178795269447 1109 44-45 0.3392855219458397 1109 46-47 0.22231025616789069 1109 48-49 0.4247157819076044 1109 50-51 0.479607856821481 1109 52-53 0.5644547104165127 1109 54-55 0.6207620134317793 1109 56-57 0.6026697954408888 1109 58-59 0.45539039566341444 1109 60-61 0.47016195133518224 1109 62-63 0.4505059209322617 1109 64-65 0.5157121500401907 1109 66-67 0.4750482209717255 1109 68-69 0.5168903210995488 1109 70-71 0.4266277644055094 1109 72-73 0.4603536846230476 1109 74-75 0.4535566206828321 1109 76-77 0.5086315578415856 1110 1 -0.430467775703832 1110 2 -0.4116637532556666 1110 3 -0.3851737966052511 1110 4 -0.3994053219120417 1110 5 -0.19082018627326391 1110 6 -0.40348421595644623 1110 7 -0.42402660455366714 1110 8 -0.30256054928248943 1110 9 -0.3072681061387925 1110 10-11 -0.4240794418278284 1110 12-13 -0.3934687859729493 1110 14-15 -0.42999028881770585 1110 16-17 -0.48981711492030655 1110 18-19 -0.3560196000995788 1110 20-21 -0.4234453112520882 1110 22-23 -0.5081183155091651 1110 24-25 -0.28892535265163843 1110 26-27 -0.23858687799778266 1110 28-29 -0.17723679987902585 1110 30-31 -0.3055206765158971 1110 32-33 -0.1217414141272215 1110 34-35 -0.09796318724250597 1110 36-37 -0.13901136400109948 1110 38-39 -0.07264554470824436 1110 40-41 0.021889114741703963 1110 42-43 -0.0814638832310095 1110 44-45 -0.09658877536388744 1110 46-47 -0.144434659631397 1110 48-49 -0.16232940295947174 1110 50-51 -0.156400872616814 1110 52-53 -0.15618952579325907 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-0.8068830482456377 2314 20-21 -0.6819856839228962 2314 22-23 -0.5390389347450011 2314 24-25 -0.5947589212569468 2314 26-27 -0.4773314667423634 2314 28-29 -0.630812933889942 2314 30-31 -0.734708517660259 2314 32-33 -0.7956815138825348 2314 34-35 -0.6921260895575827 2314 36-37 -0.8724952713980514 2314 38-39 -0.8499288592198226 2314 40-41 -0.6433282765626345 2314 42-43 -0.46892976879471604 2314 44-45 -0.5934149564292 2314 46-47 -0.5720849984230405 2314 48-49 -0.5129705190897162 2314 50-51 -0.3956701178643236 2314 52-53 -0.3184424902201357 2314 54-55 -0.47266200161942606 2314 56-57 -0.4112447657960203 2314 58-59 -0.4265793681807075 2314 60-61 -0.44835000358254007 2314 62-63 -0.45468665511510054 2314 64-65 -0.2788836163629931 2314 66-67 -0.47590473955898815 2314 68-69 -0.2337890115828749 2314 70-71 -0.008390581477922154 2314 72-73 0.04775855575603316 2314 74-75 0.01595981995523843 2314 76-77 -0.020866415863391552 2315 1 -1.2953106292037688 2315 2 -1.1507831964666124 2315 3 -1.2324750360148755 2315 4 -1.1486117853958433 2315 5 -1.0620075947684313 2315 6 -1.024314288456857 2315 7 -1.1128948353942576 2315 8 -0.9437591581891809 2315 9 -1.1541079743278644 2315 10-11 -1.256453659777506 2315 12-13 -1.1826052111621195 2315 14-15 -1.3408799136533815 2315 16-17 -1.213962355349942 2315 18-19 -1.1473458761111317 2315 20-21 -1.1819549860334675 2315 22-23 -1.1524738961499281 2315 24-25 -1.2950435087495507 2315 26-27 -1.1805173057457736 2315 28-29 -1.0889491988115196 2315 30-31 -1.250002687709717 2315 32-33 -1.275191695951122 2315 34-35 -1.2325469034489203 2315 36-37 -1.401016138739827 2315 38-39 -1.2621664825531056 2315 40-41 -1.206120267663863 2315 42-43 -1.3946017516135285 2315 44-45 -1.3427949794465945 2315 46-47 -1.2303091435771059 2315 48-49 -1.1534181785863211 2315 50-51 -0.9832366299580855 2315 52-53 -1.1257207792281214 2315 54-55 -1.240359395067653 2315 56-57 -1.2586668653871982 2315 58-59 -1.106801861667936 2315 60-61 -1.1992505953896924 2315 62-63 -1.2325645521531783 2315 64-65 -1.4665049031222104 2315 66-67 -1.3923440010761148 2315 68-69 -1.3998426835319862 2315 70-71 -1.4699316636808533 2315 72-73 -2.8305044493145353 2315 74-75 -3.319165788585277 2315 76-77 -2.8619513876213354 2316 1 -3.238098427310824 2316 2 -3.2244438595195035 2316 3 -2.5797918276409746 2316 4 -2.5798271994918025 2316 5 -3.365290334131579 2316 6 -3.00248132861163 2316 7 -3.3144861578556615 2316 8 -3.081675189702139 2316 9 -2.7402737246750917 2316 10-11 -2.749501938550601 2316 12-13 -2.878094271589937 2316 14-15 -2.940281614251205 2316 16-17 -4.043650853390318 2316 18-19 -3.844280623828915 2316 20-21 -3.317080521144053 2316 22-23 -3.2380274357591716 2316 24-25 -3.3382382904031545 2316 26-27 -3.2291391038293185 2316 28-29 -3.319824191044127 2316 30-31 -3.5115998154247343 2316 32-33 -3.5906443349903014 2316 34-35 -3.5145281967608994 2316 36-37 -3.396322224515391 2316 38-39 -3.4677954792630885 2316 40-41 -3.3396292327780372 2316 42-43 -3.6469741242044478 2316 44-45 -3.5068265909279006 2316 46-47 -3.2486557603234445 2316 48-49 -3.3827696136371763 2316 50-51 -3.7597063751819064 2316 52-53 -4.669985474161205 2316 54-55 -3.9478721881783763 2316 56-57 -3.931335093183783 2316 58-59 -3.9257634042419447 2316 60-61 -3.7578429305515613 2316 62-63 -3.7356398530662887 2316 64-65 -3.6647851037365484 2316 66-67 -3.655390062992474 2316 68-69 -3.6233122546541843 2316 70-71 -3.392561992184312 2316 72-73 -3.4021332736023275 2316 74-75 -3.471305783087921 2316 76-77 -3.4324372431236974 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 117944.0 3 9466.0 4 11596.0 5 13723.0 6 15656.0 7 16760.0 8 18879.0 9 19411.0 10 22660.0 11 24661.0 12 21110.0 13 18946.0 14 19175.0 15 19643.0 16 20275.0 17 21417.0 18 22212.0 19 23852.0 20 25410.0 21 27233.0 22 30257.0 23 34328.0 24 39394.0 25 46187.0 26 54171.0 27 63598.0 28 75372.0 29 88868.0 30 105024.0 31 126303.0 32 154116.0 33 193482.0 34 254127.0 35 339868.0 36 481330.0 37 644249.0 38 572090.0 39 153124.0 40 1513.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.02385360707521 26.446412483570114 28.0679926102259 22.461741299128775 2 22.66597685149879 26.698101901949123 27.913191726981157 22.72272951957093 3 23.00681189728289 26.82693179843108 27.516102437395457 22.65015386689057 4 22.404450917080666 26.885670475252297 28.01360165721108 22.696276950455957 5 22.92907117711367 26.63937157095859 27.775555265601465 22.65600198632627 6 22.877872781687973 26.65908977476819 27.981203362782043 22.481834080761796 7 22.852485956454686 26.266212057217547 27.87522445222509 23.006077534102676 8 22.69145178458271 26.784107640234044 27.849888655984362 22.67455191919888 9 22.661645204510396 26.4145919014734 27.961088397868444 22.96267449614776 10-11 23.109829129237404 26.597518868452795 27.602037364692787 22.690614637617006 12-13 23.170908910326908 26.35432405825383 27.629461158770308 22.845305872648954 14-15 22.835044043898602 26.545387339166837 27.816978438700513 22.80259017823405 16-17 23.130507305114094 26.420269251587296 27.761979661281455 22.687243782017152 18-19 23.044421970140085 26.35326287246187 27.661282588533116 22.94103256886493 20-21 22.981697044054748 26.73326709778055 27.506695137312075 22.77834072085262 22-23 23.012803563779578 26.726877196592053 27.22773914181498 23.032580097813394 24-25 23.094732460040447 26.81394462906378 27.177728687657403 22.91359422323837 26-27 23.064155150624448 26.826500780757573 27.08072684887511 23.028617219742873 28-29 23.226905906169275 26.804097723440528 27.05534429736528 22.913652073024917 30-31 23.124412310262432 26.658879573117627 27.1021510695304 23.114557047089537 32-33 22.954651546504063 27.054652876612202 27.165153193621304 22.825542383262427 34-35 23.121268220433134 26.75378913309337 27.120522339562775 23.004420306910724 36-37 23.126581026128314 26.724882166880352 27.376427396847962 22.772109410143372 38-39 23.037495859094566 26.762454861755515 27.355198158555982 22.844851120593933 40-41 22.898945482386956 26.802271993692457 27.199204564053165 23.099577959867425 42-43 22.877267960565337 27.097927655823597 26.984977765999147 23.039826617611922 44-45 22.80055192375223 26.785593938770514 27.352808086909242 23.061046050568013 46-47 22.805863514241835 26.82373298717068 27.23888491772598 23.131518580861506 48-49 22.983505601851945 26.775625913455592 27.206863104806423 23.03400537988604 50-51 22.842056339555832 26.9355413403237 27.06740898762849 23.154993332491973 52-53 22.950347711613954 26.88779320051178 27.08077976710082 23.08107932077345 54-55 22.947254417743267 26.84566079805024 27.103533269704606 23.10355151450189 56-57 22.81229076254443 27.12206449945491 27.08751597093464 22.978128767066018 58-59 22.901398904819363 27.037748577183628 27.07150978473271 22.98934273326429 60-61 22.934925422750947 26.96184222711776 26.912154888685542 23.191077461445754 62-63 22.99062318269527 26.78661880912773 27.122643601123546 23.100114407053454 64-65 22.949255917313447 26.815242482522354 27.036174099891802 23.199327500272396 66-67 22.921761552864297 26.94543248599532 27.163506918655905 22.96929904248448 68-69 22.902686934377208 26.749571349808082 27.216686330953305 23.13105538486141 70-71 22.820885414052807 26.69721426764585 27.17201756613789 23.30988275216345 72-73 22.981779920198015 26.57218466648285 27.14495881545712 23.301076597862018 74-75 22.90086671142546 26.525099236734125 27.04174382825605 23.53229022358437 76-77 22.73307620542494 26.4401451184174 27.420405651540086 23.40637302461757 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5304.0 1 3370.5 2 1381.5 3 1437.0 4 1548.0 5 1665.5 6 1852.5 7 2020.0 8 2299.0 9 2480.0 10 2504.5 11 2651.0 12 2885.5 13 2998.0 14 3137.5 15 3476.5 16 4052.0 17 4428.0 18 4893.0 19 6079.0 20 7988.0 21 10675.0 22 12174.0 23 14307.0 24 19093.0 25 25742.0 26 29738.0 27 33839.5 28 43208.0 29 52338.5 30 56202.0 31 63166.0 32 76451.5 33 88949.0 34 101389.0 35 107653.0 36 114495.5 37 127930.0 38 137977.5 39 141433.0 40 146430.0 41 158453.0 42 175014.5 43 184550.0 44 185265.5 45 186360.5 46 189851.0 47 194637.0 48 196312.0 49 197035.0 50 196585.5 51 189220.0 52 183027.0 53 180196.0 54 169260.5 55 151566.0 56 132411.0 57 122846.0 58 116331.5 59 98379.0 60 79608.5 61 72276.0 62 63306.5 63 46214.0 64 33395.5 65 28700.0 66 25015.5 67 19436.5 68 15063.0 69 10329.0 70 8074.0 71 6866.0 72 4853.5 73 3682.0 74 3315.0 75 2729.0 76 1784.0 77 1155.5 78 886.0 79 734.0 80 476.0 81 328.0 82 252.5 83 219.0 84 186.0 85 140.0 86 120.5 87 114.0 88 101.0 89 87.0 90 77.0 91 68.0 92 63.5 93 59.0 94 56.0 95 50.0 96 47.0 97 54.5 98 69.0 99 136.5 100 197.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00896786010138242 2 0.012134477368819714 3 0.012970464327423159 4 0.014135779481840085 5 0.011273157472076769 6 0.00863853190556894 7 0.015174429945559516 8 0.016542408605092426 9 0.016162414532999953 10-11 0.019329031800437248 12-13 0.015187096414629266 14-15 0.007308552653245277 16-17 0.005813909303014873 18-19 0.0177457231667186 20-21 0.023027640768804005 22-23 0.02111500393927188 24-25 0.018252381929508565 26-27 0.023876294196477202 28-29 0.007891210230453739 30-31 0.007612547910919257 32-33 0.009689848838358122 34-35 0.010614501080449812 36-37 0.010855163992775047 38-39 0.013363124868585383 40-41 0.010437170513473324 42-43 0.0138571171623056 44-45 0.012869132574865166 46-47 0.02338230190275698 48-49 0.022242319686479557 50-51 0.03617543566320366 52-53 0.03950671702854769 54-55 0.027853565484378444 56-57 0.020456347547644922 58-59 0.015009765847652777 60-61 0.019012370073693517 62-63 0.025206273448800866 64-65 0.024319620613918423 66-67 0.02713157674740274 68-69 0.0408746956880806 70-71 0.053503165350620524 72-73 0.08340869882429834 74-75 0.08425735225197153 76-77 0.08812062531824504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3947430.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.7530346279482 #Duplication Level Percentage of deduplicated Percentage of total 1 75.53421859584762 39.84659249181758 2 12.943994268268433 13.656699557158552 3 4.739906020994794 7.501332793762755 4 2.213469387001039 4.670689088814764 5 1.2042996972836642 3.1765231816616337 6 0.729804518943976 2.309964183569079 7 0.4765916261727437 1.7599158191216622 8 0.3548362630185878 1.497495173621704 9 0.25824479039681153 1.2260876733261201 >10 1.372023644748771 13.19457244083105 >50 0.10882169497555619 3.9848575122471606 >100 0.06027002401653953 5.627199804083797 >500 0.0028927137282786174 1.009572366241038 >1k 5.785427106486801E-4 0.38650889317310444 >5k 4.821189255405624E-5 0.15198902057001087 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5976 0.15138963832164218 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.5332938139498357E-5 0.0 2 0.0 0.0 0.0 5.0665876278996715E-5 0.0 3 2.5332938139498357E-5 0.0 0.0 5.0665876278996715E-5 0.0 4 7.599881441849507E-5 0.0 0.0 4.053270102319737E-4 0.0 5 7.599881441849507E-5 0.0 0.0 9.119857730219409E-4 0.0 6 7.599881441849507E-5 0.0 0.0 0.001773305669764885 0.0 7 7.599881441849507E-5 0.0 0.0 0.003901272473482747 0.0 8 7.599881441849507E-5 0.0 0.0 0.005978573400921612 2.5332938139498357E-5 9 1.0133175255799343E-4 0.0 0.0 0.008663864843708438 2.5332938139498357E-5 10 1.0133175255799343E-4 0.0 0.0 0.011019828090681786 2.5332938139498357E-5 11 1.266646906974918E-4 0.0 0.0 0.01481976881160654 2.5332938139498357E-5 12 1.266646906974918E-4 0.0 0.0 0.019683692934390224 2.5332938139498357E-5 13 1.5199762883699014E-4 0.0 0.0 0.026042260407404313 2.5332938139498357E-5 14 1.773305669764885E-4 0.0 0.0 0.034756791127391745 2.5332938139498357E-5 15 2.0266350511598686E-4 0.0 0.0 0.044737968754354096 2.5332938139498357E-5 16 2.0266350511598686E-4 0.0 0.0 0.05520047220596692 2.5332938139498357E-5 17 2.7866231953448193E-4 0.0 0.0 0.0649536533896738 2.5332938139498357E-5 18 2.7866231953448193E-4 0.0 0.0 0.07333885591384774 2.5332938139498357E-5 19 3.039952576739803E-4 0.0 0.0 0.08362402879848407 2.5332938139498357E-5 20 3.2932819581347865E-4 0.0 0.0 0.0939852004975389 2.5332938139498357E-5 21 3.2932819581347865E-4 0.0 0.0 0.10746232358775203 2.5332938139498357E-5 22 3.54661133952977E-4 0.0 0.0 0.12068611729657017 2.5332938139498357E-5 23 4.053270102319737E-4 0.0 0.0 0.13441656976817828 5.0665876278996715E-5 24 4.306599483714721E-4 0.0 0.0 0.1481470222397864 1.0133175255799343E-4 25 4.306599483714721E-4 0.0 0.0 0.1633721180616249 1.0133175255799343E-4 26 4.306599483714721E-4 0.0 0.0 0.1806491818727628 1.0133175255799343E-4 27 4.306599483714721E-4 0.0 0.0 0.1974195869211107 1.0133175255799343E-4 28 4.306599483714721E-4 0.0 0.0 0.21424065784573762 1.0133175255799343E-4 29 4.306599483714721E-4 0.0 0.0 0.23108706170850402 1.773305669764885E-4 30 4.5599288651097043E-4 0.0 0.0 0.24925077835452433 1.773305669764885E-4 31 4.5599288651097043E-4 0.0 0.0 0.2694411300517045 1.773305669764885E-4 32 5.573246390689639E-4 0.0 0.0 0.2901888063879537 1.773305669764885E-4 33 5.573246390689639E-4 0.0 0.0 0.31344444360001317 1.773305669764885E-4 34 5.573246390689639E-4 0.0 0.0 0.33811872534788456 1.773305669764885E-4 35 5.573246390689639E-4 0.0 0.0 0.3638823234357544 2.0266350511598686E-4 36 5.573246390689639E-4 0.0 0.0 0.39238187884269005 2.0266350511598686E-4 37 5.573246390689639E-4 0.0 0.0 0.42262940698125107 2.0266350511598686E-4 38 5.573246390689639E-4 0.0 0.0 0.45249694104771965 2.2799644325548522E-4 39 5.573246390689639E-4 0.0 0.0 0.48350445733046565 2.2799644325548522E-4 40 5.573246390689639E-4 0.0 0.0 0.5163866110355345 2.2799644325548522E-4 41 5.573246390689639E-4 0.0 0.0 0.5525113808224592 2.2799644325548522E-4 42 5.573246390689639E-4 0.0 0.0 0.5889401458670578 2.2799644325548522E-4 43 5.573246390689639E-4 0.0 0.0 0.6321834712711815 2.2799644325548522E-4 44 5.573246390689639E-4 0.0 0.0 0.6774534317264651 2.2799644325548522E-4 45 5.573246390689639E-4 0.0 0.0 0.7244460319752345 2.2799644325548522E-4 46 5.573246390689639E-4 0.0 0.0 0.7768092151095777 2.2799644325548522E-4 47 5.826575772084622E-4 0.0 0.0 0.8310217027281042 2.2799644325548522E-4 48 5.826575772084622E-4 2.5332938139498357E-5 0.0 0.8834608846768657 2.533293813949836E-4 49 6.079905153479606E-4 2.5332938139498357E-5 0.0 0.9388386874498091 2.533293813949836E-4 50 6.079905153479606E-4 2.5332938139498357E-5 0.0 0.9960657947069359 2.533293813949836E-4 51 6.079905153479606E-4 2.5332938139498357E-5 0.0 1.0586128189733572 2.533293813949836E-4 52 6.079905153479606E-4 2.5332938139498357E-5 0.0 1.1266317578779104 2.533293813949836E-4 53 6.079905153479606E-4 2.5332938139498357E-5 0.0 1.1968293294624603 2.533293813949836E-4 54 6.079905153479606E-4 2.5332938139498357E-5 0.0 1.2730308073860714 2.533293813949836E-4 55 6.079905153479606E-4 2.5332938139498357E-5 0.0 1.3532602224738628 2.533293813949836E-4 56 7.346552060454524E-4 2.5332938139498357E-5 0.0 1.443445482250477 2.533293813949836E-4 57 7.346552060454524E-4 2.5332938139498357E-5 0.0 1.5313254446563966 2.7866231953448193E-4 58 7.346552060454524E-4 2.5332938139498357E-5 0.0 1.627717274277188 2.7866231953448193E-4 59 7.346552060454524E-4 2.5332938139498357E-5 0.0 1.7222344664756564 2.7866231953448193E-4 60 7.346552060454524E-4 2.5332938139498357E-5 0.0 1.821134256972258 2.7866231953448193E-4 61 7.853210823244491E-4 2.5332938139498357E-5 0.0 1.9271526030860586 2.7866231953448193E-4 62 7.853210823244491E-4 2.5332938139498357E-5 0.0 2.03666689466311 2.7866231953448193E-4 63 7.853210823244491E-4 2.5332938139498357E-5 0.0 2.156314361495961 2.7866231953448193E-4 64 7.853210823244491E-4 2.5332938139498357E-5 0.0 2.282396394616244 2.7866231953448193E-4 65 7.853210823244491E-4 2.5332938139498357E-5 0.0 2.4178262819100023 2.7866231953448193E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGATA 50 0.0067296578 28.400198 20 CGCGGAA 450 0.0 23.66623 59 CGATCGT 540 0.0 22.34776 1 CTCGCGC 570 0.0 21.171293 36 ATTCGCG 495 0.0 20.798122 56 TCGCGCT 570 0.0 19.926933 37 GACGTAT 535 0.0 19.904663 39 GCGTAAG 750 0.0 19.876863 38 TTGCGTA 755 0.0 19.744478 36 TGCGTAA 755 0.0 19.275349 37 TCGCGGA 555 0.0 19.188107 58 CCTCGCG 605 0.0 18.77366 35 CGCGCTT 610 0.0 18.620014 38 CGGTTAC 385 0.0 18.43748 4 CGTAAGT 790 0.0 18.42231 39 CGTATTA 620 0.0 18.321081 41 CAATTCG 655 0.0 17.885166 54 AATTCGC 600 0.0 17.749224 55 ACGTATT 620 0.0 17.176233 40 CCGAACG 150 2.8384727E-4 16.565943 55 >>END_MODULE