FastQCFastQC Report
Thu 26 May 2016
SRR936169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947430
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82700.2095033984136514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60180.15245362172350113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41410.1049036968356627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14200.045.47891100-101
CGGTATC11750.038.75183100-101
GGTATCA86350.036.453411
GCGTATC4600.032.34825100-101
CGTACAT20700.030.766783100-101
GTATCAA166900.029.1992971
TCGTATC6350.027.651388100-101
CGAGTAC14550.027.408195100-101
TCGAGTA7200.025.627007100-101
TCGGTAT4750.025.061384100-101
CCGTATC9300.024.640326100-101
CCGTACA10200.024.508558100-101
AGTATCA40500.024.102243100-101
CCGGTAT5500.023.267216100-101
ACGTATC5400.023.146973100-101
CCGAGTA6750.022.926527100-101
ACCGTAT4550.022.8923298-99
ATCAACG211850.022.7200813
CCGTATA4200.022.674585100-101
TATCAAC213850.022.5922052
Produced by FastQC (version 0.11.5)