FastQCFastQC Report
Thu 26 May 2016
SRR936168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3967452
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58900.14845800276852752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCG2550.027.84159510
CGCGGAA5050.025.30967759
ATTCGCG5050.025.30680756
AATTCGC5150.024.81634955
CTCGCGC5350.024.54663736
CGATCGT5800.024.4783171
TCGCGGA5750.022.84566958
CGCGCTT6300.022.53616138
CGGTTAC5350.021.8943254
CAATTCG6000.021.30096854
TCGCGCT6300.020.84568637
ACGATCG2750.020.65678659
CGTTCGG3450.020.5783111
CCTCGCG6450.020.35987735
CGCTCCG7950.020.08993325
GATCGTT7650.018.5587232
AACTCGA7750.018.32133513
ATCGTTC7750.018.3197173
GGTTGCG4300.018.160646
GACGCTC8150.017.85768123
Produced by FastQC (version 0.11.5)