##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936168_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3967452 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.25454649482842 39.0 36.0 41.0 29.0 41.0 2 36.12323627355794 39.0 36.0 41.0 28.0 41.0 3 35.98592698790055 39.0 35.0 41.0 27.0 41.0 4 35.87225226669409 39.0 35.0 41.0 27.0 41.0 5 35.801002507402735 39.0 35.0 41.0 27.0 41.0 6 35.71111887428002 39.0 35.0 40.0 27.0 41.0 7 35.570210805322915 39.0 35.0 40.0 27.0 41.0 8 35.443578397419806 38.0 35.0 40.0 27.0 41.0 9 35.615995102146165 39.0 35.0 40.0 27.0 41.0 10-11 35.84337126195856 39.0 35.0 41.0 28.0 41.0 12-13 35.769431363000734 39.0 35.0 41.0 27.0 41.0 14-15 35.61692164139604 39.0 35.0 41.0 26.5 41.0 16-17 35.467073073599884 39.0 35.0 41.0 26.0 41.0 18-19 35.31610224900011 39.0 35.0 41.0 25.5 41.0 20-21 35.12802536741465 39.0 35.0 41.0 25.0 41.0 22-23 34.86405620030186 39.0 34.0 41.0 24.0 41.0 24-25 34.645344921627284 39.0 34.0 41.0 23.5 41.0 26-27 33.69520173148913 38.0 33.0 39.5 22.0 40.5 28-29 33.707425445852905 38.0 33.0 39.5 22.0 40.5 30-31 34.109653878610246 38.0 33.0 40.0 22.0 41.0 32-33 33.98498381328873 38.0 33.0 40.0 21.5 41.0 34-35 33.72891493079186 38.0 33.0 40.0 20.0 41.0 36-37 33.41413620126973 37.5 33.0 40.0 18.5 41.0 38-39 33.10226235881366 37.0 32.0 40.0 17.5 41.0 40-41 32.73865329687669 36.5 32.0 40.0 13.5 41.0 42-43 32.368640376745574 36.0 32.0 39.0 12.0 41.0 44-45 32.001730203667236 35.5 31.0 39.0 8.0 40.5 46-47 31.58123337093933 35.0 31.0 38.5 7.0 40.0 48-49 31.16089432209892 35.0 31.0 37.5 4.0 40.0 50-51 30.707687074727055 35.0 30.0 37.0 2.0 39.0 52-53 30.223864460111933 35.0 30.0 36.5 2.0 39.0 54-55 29.758825815662043 34.0 29.5 36.0 2.0 38.5 56-57 29.32468445743011 34.0 29.0 35.5 2.0 37.0 58-59 28.90416594832149 34.0 29.0 35.0 2.0 37.0 60-61 28.548909854485952 34.0 29.0 35.0 2.0 36.5 62-63 28.1772171408753 34.0 28.0 35.0 2.0 36.0 64-65 27.806738430609876 34.0 27.0 35.0 2.0 36.0 66-67 27.453213800696265 34.0 26.5 35.0 2.0 35.0 68-69 27.089521309898643 33.0 26.0 35.0 2.0 35.0 70-71 26.638258887568142 33.0 25.0 35.0 2.0 35.0 72-73 26.15855503733883 33.0 24.5 35.0 2.0 35.0 74-75 25.58340932669129 33.0 22.0 35.0 2.0 35.0 76-77 23.83692783176709 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9450298946153666 1101 2 0.9141815810221985 1101 3 1.0405228843330434 1101 4 0.93763907401447 1101 5 0.9682854682010458 1101 6 1.0136746063287205 1101 7 1.0267520257783502 1101 8 0.8965762841433644 1101 9 0.9497186128447908 1101 10-11 0.9545172600747165 1101 12-13 1.2319924628432801 1101 14-15 1.2664458606817561 1101 16-17 1.2609956089001884 1101 18-19 1.3073562102682743 1101 20-21 1.2217470008682838 1101 22-23 1.1941336633119164 1101 24-25 1.2698669057682181 1101 26-27 1.305321808938821 1101 28-29 1.3104300627231353 1101 30-31 1.3268149806487202 1101 32-33 1.4350041755395253 1101 34-35 1.4798486528129189 1101 36-37 1.4378137901854586 1101 38-39 1.4968005360353658 1101 40-41 1.3575608522540037 1101 42-43 1.3434193677948087 1101 44-45 1.252118263482636 1101 46-47 1.1580788608763442 1101 48-49 1.2291156814568076 1101 50-51 1.1299083734221718 1101 52-53 1.180440214747243 1101 54-55 1.2326451387309127 1101 56-57 1.3052141787728644 1101 58-59 1.2944372837572047 1101 60-61 1.2095297805137015 1101 62-63 1.1808885864135448 1101 64-65 1.1714674409815267 1101 66-67 1.2469730432551174 1101 68-69 1.1228325420662166 1101 70-71 1.01238917514247 1101 72-73 0.9813639038682602 1101 74-75 1.0901040749088686 1101 76-77 1.2642338674276772 1102 1 0.9061167308589688 1102 2 0.8854189477674907 1102 3 0.9470685106369672 1102 4 0.9274722560720292 1102 5 0.842987667153615 1102 6 0.9288051945866584 1102 7 0.8880210345789976 1102 8 0.8849672873559342 1102 9 0.8903286811483966 1102 10-11 0.8856640785205911 1102 12-13 1.0354304454512544 1102 14-15 1.104363615028646 1102 16-17 1.0196922385351357 1102 18-19 1.0902999035984635 1102 20-21 1.0416654057256878 1102 22-23 0.8851016851430131 1102 24-25 1.0090847433784873 1102 26-27 0.7784939262222181 1102 28-29 1.060677952635828 1102 30-31 1.1550106135416556 1102 32-33 1.0902118872972366 1102 34-35 1.0196003729887693 1102 36-37 1.007876684384712 1102 38-39 1.0549225151469699 1102 40-41 1.0293121617889156 1102 42-43 1.0979582580080844 1102 44-45 0.974184932419675 1102 46-47 1.0161323340250163 1102 48-49 0.9482377785289664 1102 50-51 1.0209883343915394 1102 52-53 0.9608832923628228 1102 54-55 0.993717513701359 1102 56-57 0.9975192514222542 1102 58-59 1.1190918789624824 1102 60-61 0.9439571363069241 1102 62-63 0.9338863392402779 1102 64-65 0.9302751213488953 1102 66-67 0.9627179641647814 1102 68-69 0.9115261955415335 1102 70-71 0.9602311139204467 1102 72-73 1.0505035963120228 1102 74-75 0.9460357387301706 1102 76-77 0.909826806772756 1103 1 0.37208331153129137 1103 2 0.46426415843212254 1103 3 0.4027680285869977 1103 4 0.4226657405724694 1103 5 0.44980800212695016 1103 6 0.5246023467587548 1103 7 0.5197770924055618 1103 8 0.508104270288996 1103 9 0.4722059367996394 1103 10-11 0.4472232182495617 1103 12-13 0.5710731964458162 1103 14-15 0.5271876634919579 1103 16-17 0.47224655373914715 1103 18-19 0.3249336107861609 1103 20-21 0.43248145812373906 1103 22-23 0.29581212779631016 1103 24-25 0.3047139876185909 1103 26-27 0.32510454770017816 1103 28-29 0.30130496113400085 1103 30-31 0.2854041284118196 1103 32-33 0.27127249162281686 1103 34-35 0.42474811066291096 1103 36-37 0.43046029429697796 1103 38-39 0.3384317340169787 1103 40-41 0.392818052203765 1103 42-43 0.3693707007256535 1103 44-45 0.32357400197002306 1103 46-47 0.26536471078836854 1103 48-49 0.20603298095920763 1103 50-51 0.1977960322005714 1103 52-53 0.2533034158585039 1103 54-55 0.20374011957500215 1103 56-57 0.2665171067339749 1103 58-59 0.11803185343830691 1103 60-61 0.01768196703353553 1103 62-63 -0.004320120336235078 1103 64-65 -0.047982626695795005 1103 66-67 -0.017843191444868012 1103 68-69 -0.019866195940309694 1103 70-71 -0.0401964287822949 1103 72-73 -0.015927017924671105 1103 74-75 0.05606019760115899 1103 76-77 0.05614516823451865 1104 1 0.449088900314905 1104 2 0.44180737781164936 1104 3 0.49213872259668534 1104 4 0.5471970959860499 1104 5 0.4489160147294555 1104 6 0.4678724345587639 1104 7 0.470375970731574 1104 8 0.39271674813029733 1104 9 0.4090717752627242 1104 10-11 0.5679812717840704 1104 12-13 0.6607819906229864 1104 14-15 0.7109485152342216 1104 16-17 0.7110925407380293 1104 18-19 0.7232796688022844 1104 20-21 0.6575985926768695 1104 22-23 0.7202517382503473 1104 24-25 0.7079657737039824 1104 26-27 0.47739614889623994 1104 28-29 0.5172516858409821 1104 30-31 0.6878137371024167 1104 32-33 0.7106710420875757 1104 34-35 0.7550802815827424 1104 36-37 0.68376945137463 1104 38-39 0.6371468790876875 1104 40-41 0.7678023409469787 1104 42-43 0.8147019770916089 1104 44-45 0.6718498295708173 1104 46-47 0.585589435960415 1104 48-49 0.5868706710637248 1104 50-51 0.50074506127023 1104 52-53 0.5242293071519377 1104 54-55 0.5249380815455531 1104 56-57 0.5956985483982251 1104 58-59 0.46365447948513605 1104 60-61 0.4809351525267296 1104 62-63 0.2552122815939448 1104 64-65 0.2842719401722924 1104 66-67 0.28224809424957087 1104 68-69 0.038755314429995025 1104 70-71 -0.03566895280810556 1104 72-73 0.006640425325525001 1104 74-75 -0.03632357945318532 1104 76-77 0.0352831857072573 1105 1 0.13871461887710268 1105 2 0.3467552545354238 1105 3 0.23272043374387152 1105 4 0.25597307737258745 1105 5 -0.03476603172853743 1105 6 0.16041331449769558 1105 7 0.12675033790356593 1105 8 0.19687168956370726 1105 9 0.13561123243806605 1105 10-11 0.298340803495158 1105 12-13 0.3459842819124006 1105 14-15 0.399250786706709 1105 16-17 0.43325276284127057 1105 18-19 0.23486783114945808 1105 20-21 0.07202748533891423 1105 22-23 0.24477390428302925 1105 24-25 0.2750636467075225 1105 26-27 0.20922084751304482 1105 28-29 0.31479373153968737 1105 30-31 0.48748439588621295 1105 32-33 0.5598477652405407 1105 34-35 0.4656244810383825 1105 36-37 0.5775983517620347 1105 38-39 0.48207495611541873 1105 40-41 0.6729505733152692 1105 42-43 0.6253591349924648 1105 44-45 0.3885519836126363 1105 46-47 0.43945185749026905 1105 48-49 0.2678883518530597 1105 50-51 0.3720277236628178 1105 52-53 0.3137926067152321 1105 54-55 0.250433357187287 1105 56-57 0.49350053964445806 1105 58-59 0.5106040217314565 1105 60-61 0.5464578087814367 1105 62-63 0.351434802509214 1105 64-65 0.469263937541168 1105 66-67 0.31885570083377246 1105 68-69 0.34985750833092766 1105 70-71 0.19683787594698288 1105 72-73 -0.275621454740552 1105 74-75 -0.44472689536560495 1105 76-77 -0.35625401448559657 1106 1 0.12070416114934801 1106 2 0.209756563204877 1106 3 0.12396952597335797 1106 4 0.2206460712129612 1106 5 0.1231103904452695 1106 6 0.22172528470062502 1106 7 -0.0778506315477756 1106 8 0.1161659356464213 1106 9 0.1947199101428012 1106 10-11 -0.018467637898545775 1106 12-13 0.31322086857898057 1106 14-15 0.2229753955593594 1106 16-17 0.14971084049251715 1106 18-19 0.31404355570096243 1106 20-21 0.2897534356306153 1106 22-23 0.30511401827892115 1106 24-25 0.1448281099891986 1106 26-27 0.16320712286852057 1106 28-29 0.3775854535711076 1106 30-31 0.538545099967294 1106 32-33 0.4292489551466616 1106 34-35 0.26179379517085266 1106 36-37 0.35590060815976443 1106 38-39 0.5691468636967087 1106 40-41 0.5015530282332961 1106 42-43 0.5031949598717631 1106 44-45 0.4560630552416711 1106 46-47 0.4275382283532423 1106 48-49 0.40786990178070326 1106 50-51 0.3345998299758257 1106 52-53 0.3184916074541313 1106 54-55 0.272958982214373 1106 56-57 0.1945375188061682 1106 58-59 0.10594357811576671 1106 60-61 -0.11117716889824081 1106 62-63 -0.06875670932113565 1106 64-65 -0.054656938696545865 1106 66-67 -0.13576650939054957 1106 68-69 -0.012076905711793984 1106 70-71 -0.18170097557399956 1106 72-73 -0.2820571777170038 1106 74-75 -0.12264776922487997 1106 76-77 -0.2937548403321344 1107 1 -0.13328805012221778 1107 2 -0.10933296094511036 1107 3 -0.009812825191254149 1107 4 -0.08293315357126119 1107 5 -0.06082987713689647 1107 6 -0.15212019707671232 1107 7 -0.10773751418678046 1107 8 -0.30529562201708416 1107 9 -0.2451198158648964 1107 10-11 -0.15957792462894105 1107 12-13 -0.13552300378120918 1107 14-15 -0.2888902293571718 1107 16-17 -0.34113853359450275 1107 18-19 -0.2121056849442411 1107 20-21 -0.2992950261417491 1107 22-23 -0.14167477486880387 1107 24-25 -0.22030821330364603 1107 26-27 -0.5093086662050723 1107 28-29 -0.4101272032666827 1107 30-31 -0.09922124434339707 1107 32-33 -0.0132274408560491 1107 34-35 -0.13765615541766607 1107 36-37 -0.18061066145844507 1107 38-39 -0.1284103241840171 1107 40-41 -0.3194616560160526 1107 42-43 -0.2190194294496024 1107 44-45 -0.2511926121831145 1107 46-47 -0.2469095264548713 1107 48-49 -0.2193335189113057 1107 50-51 -0.30342307393990353 1107 52-53 -0.31491322357093665 1107 54-55 -0.24477374225859094 1107 56-57 -0.3327296437216454 1107 58-59 -0.29400716449459807 1107 60-61 -0.2569380157966208 1107 62-63 -0.2254938258685364 1107 64-65 -0.16448576541531423 1107 66-67 -0.09097753287589683 1107 68-69 -0.20966754393141684 1107 70-71 -0.24957883984754758 1107 72-73 -0.18955074703380959 1107 74-75 -0.3101303699123257 1107 76-77 -0.2547631093072731 1108 1 0.1365477064721219 1108 2 0.28388858214080415 1108 3 0.3291985615984174 1108 4 0.3392183123528483 1108 5 0.23528283080390366 1108 6 0.27781660833878163 1108 7 0.2472396331068225 1108 8 0.32739036256285914 1108 9 0.23171256927688688 1108 10-11 0.20983698800334594 1108 12-13 0.2462308074541184 1108 14-15 0.324039064156338 1108 16-17 0.31172961919234154 1108 18-19 0.23412957148833868 1108 20-21 0.21254840540799336 1108 22-23 0.21131136706692644 1108 24-25 0.3602667893636635 1108 26-27 0.1408611370146744 1108 28-29 0.32430759388439867 1108 30-31 0.3738073505508055 1108 32-33 0.3390925509334508 1108 34-35 0.3673976085260122 1108 36-37 0.3409000845005039 1108 38-39 0.42524308158359503 1108 40-41 0.29025693100359007 1108 42-43 0.3020062787221818 1108 44-45 0.18802353278848472 1108 46-47 0.2204728668026661 1108 48-49 0.31934432210385566 1108 50-51 0.1922753306346472 1108 52-53 0.4577791367055113 1108 54-55 0.5183377526878026 1108 56-57 0.41326336454756074 1108 58-59 0.35654673721384356 1108 60-61 0.39211253334885043 1108 62-63 0.27707217354250346 1108 64-65 0.31889253784706995 1108 66-67 0.39949011787868116 1108 68-69 0.3781032216603961 1108 70-71 0.42321130325921175 1108 72-73 0.41950415102334304 1108 74-75 0.43772529298228946 1108 76-77 0.46371278788957326 1109 1 -0.07516705340929519 1109 2 -0.12728177611896996 1109 3 -0.20833193657134075 1109 4 -0.07305197137334574 1109 5 -0.0786975283778304 1109 6 -0.14814006650314582 1109 7 -0.049847060217693695 1109 8 -0.08445766822550382 1109 9 -0.0058023834753839765 1109 10-11 -0.12468396995410558 1109 12-13 -0.05976183168975524 1109 14-15 0.10256605234090443 1109 16-17 0.13173353705355595 1109 18-19 -0.028254011474054153 1109 20-21 -0.012411099115929858 1109 22-23 -0.14487223110440084 1109 24-25 -0.2469823061603833 1109 26-27 -0.33857835065828823 1109 28-29 -0.09862167055062088 1109 30-31 -0.046209641122757716 1109 32-33 -0.09820775147723282 1109 34-35 -0.008282488194502946 1109 36-37 0.03265779525088419 1109 38-39 0.09089409595420506 1109 40-41 0.09097177549466551 1109 42-43 0.16278810248903142 1109 44-45 0.036991083977781614 1109 46-47 0.029699852105729008 1109 48-49 0.04149151928221073 1109 50-51 -0.10664328705532711 1109 52-53 -0.08787933285823613 1109 54-55 -0.048689486909172075 1109 56-57 0.027532736349350273 1109 58-59 0.06972506664491718 1109 60-61 0.08611191043094024 1109 62-63 -0.008092359192897192 1109 64-65 0.06192186191261939 1109 66-67 0.13000868449697123 1109 68-69 -0.06067432366006287 1109 70-71 0.03417872683047918 1109 72-73 0.0760044756050533 1109 74-75 0.02654958161731713 1109 76-77 -0.029104017899708623 1110 1 -0.3830903949997335 1110 2 -0.35593251537484605 1110 3 -0.5964883940133916 1110 4 -0.37829892140250365 1110 5 -0.4772390439329399 1110 6 -0.5099769194201258 1110 7 -0.5312138234411492 1110 8 -0.658600475470017 1110 9 -0.5336214189944641 1110 10-11 -0.4776729550588641 1110 12-13 -0.39026648750147785 1110 14-15 -0.41697822434122145 1110 16-17 -0.3881181360642998 1110 18-19 -0.5991856423918733 1110 20-21 -0.6031616387767045 1110 22-23 -0.5723925578014928 1110 24-25 -0.5286033173653308 1110 26-27 -0.5608901942484152 1110 28-29 -0.40730566891750897 1110 30-31 -0.29472685990185 1110 32-33 -0.3582233035545741 1110 34-35 -0.3566212511377742 1110 36-37 -0.337519404716339 1110 38-39 -0.3662957696143394 1110 40-41 -0.3784094107136653 1110 42-43 -0.37738250773585946 1110 44-45 -0.3350817521195921 1110 46-47 -0.2549911988671951 1110 48-49 -0.3360533242057606 1110 50-51 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-0.508094403695388 2314 22-23 -0.4199194044218473 2314 24-25 -0.37401067187497716 2314 26-27 -0.36516974105514777 2314 28-29 -0.48492585249074693 2314 30-31 -0.5359924775985974 2314 32-33 -0.7055411484907381 2314 34-35 -0.7377138731318169 2314 36-37 -1.256199445532765 2314 38-39 -0.7971069290140704 2314 40-41 -0.6244129418718529 2314 42-43 -0.50094729290835 2314 44-45 -0.6211264920113564 2314 46-47 -0.46384812905884587 2314 48-49 -0.3259198631252822 2314 50-51 -0.2780044920928866 2314 52-53 -0.2139901226706229 2314 54-55 -0.3205495815644319 2314 56-57 -0.2982660151714782 2314 58-59 -0.26759789486139596 2314 60-61 -0.21679808340099171 2314 62-63 -0.26149848787727237 2314 64-65 -0.11357548460185996 2314 66-67 -0.1907533835482269 2314 68-69 -0.14179414605974827 2314 70-71 -0.043207573629540974 2314 72-73 0.0882100417305054 2314 74-75 0.11473200814930351 2314 76-77 -0.03949975215385848 2315 1 -1.3654403216039128 2315 2 -1.1546109116041023 2315 3 -1.0563395137187825 2315 4 -0.9775016540753398 2315 5 -1.2218225028395366 2315 6 -1.183448565554876 2315 7 -1.6019743859398048 2315 8 -1.4658852751956104 2315 9 -1.1991694678920766 2315 10-11 -1.2807642023108414 2315 12-13 -1.1456591825390632 2315 14-15 -1.1103425376754075 2315 16-17 -1.0910803005707876 2315 18-19 -1.130998063993971 2315 20-21 -1.1581100306955747 2315 22-23 -1.2057365083073037 2315 24-25 -1.3084153050543748 2315 26-27 -1.179402669834154 2315 28-29 -1.2372659386206593 2315 30-31 -1.2825046118937138 2315 32-33 -1.2023584299977514 2315 34-35 -1.3599398982105058 2315 36-37 -1.276936956286363 2315 38-39 -1.2580108301125286 2315 40-41 -1.4208355893395677 2315 42-43 -1.4218800463225563 2315 44-45 -1.3433161464995038 2315 46-47 -1.2401448733900367 2315 48-49 -1.1614360560631418 2315 50-51 -1.1551685616847394 2315 52-53 -1.2372621207982988 2315 54-55 -1.3268123706079997 2315 56-57 -1.4426078446384452 2315 58-59 -1.2692475088214756 2315 60-61 -1.370184969405308 2315 62-63 -1.2792963424890722 2315 64-65 -1.0896938486470553 2315 66-67 -1.204638578743225 2315 68-69 -1.415096542180322 2315 70-71 -1.4047393173051042 2315 72-73 -1.3790459698738466 2315 74-75 -1.3700605812383024 2315 76-77 -1.1682090368389915 2316 1 -2.142589749217912 2316 2 -1.664840685959291 2316 3 -1.9867393355475258 2316 4 -2.9782536320496433 2316 5 -2.2650017557379982 2316 6 -2.192266798125196 2316 7 -1.8832093855514742 2316 8 -1.6762975235496782 2316 9 -2.003910436268029 2316 10-11 -1.9210863015276658 2316 12-13 -2.4796215636150194 2316 14-15 -3.560308345787888 2316 16-17 -2.575413217792324 2316 18-19 -2.133515409577754 2316 20-21 -1.8468967964460958 2316 22-23 -2.3339204197996963 2316 24-25 -2.2053055346176507 2316 26-27 -2.1958905473419534 2316 28-29 -2.820940915706551 2316 30-31 -3.0435066736902385 2316 32-33 -3.021474874820811 2316 34-35 -2.8569496573085615 2316 36-37 -2.880906137854076 2316 38-39 -2.8758320965194244 2316 40-41 -2.5161624635136945 2316 42-43 -2.8461607515711655 2316 44-45 -2.8315652323028715 2316 46-47 -2.7793359477243094 2316 48-49 -2.6148952007490855 2316 50-51 -2.628183436441219 2316 52-53 -2.79045487455366 2316 54-55 -2.6769421157641027 2316 56-57 -2.52749513859748 2316 58-59 -2.711793122585476 2316 60-61 -2.5099574817256176 2316 62-63 -2.5712265969919947 2316 64-65 -2.512688522181506 2316 66-67 -2.548277818744296 2316 68-69 -2.6232186241320683 2316 70-71 -2.462922435902918 2316 72-73 -2.486550405889009 2316 74-75 -2.7787172581548703 2316 76-77 -2.9730296194081376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 119097.0 3 9227.0 4 11719.0 5 14556.0 6 16137.0 7 17345.0 8 19172.0 9 19706.0 10 22732.0 11 25128.0 12 21150.0 13 19280.0 14 19405.0 15 20388.0 16 20855.0 17 21617.0 18 22499.0 19 23761.0 20 26067.0 21 27617.0 22 30725.0 23 34821.0 24 39860.0 25 46707.0 26 54945.0 27 64434.0 28 75951.0 29 88867.0 30 105640.0 31 126514.0 32 153916.0 33 193351.0 34 254002.0 35 340302.0 36 481538.0 37 649238.0 38 578469.0 39 149374.0 40 1340.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.033142575990386 26.486359640101792 28.04584124794825 22.434656535959576 2 22.659998190124178 26.70073501628258 27.932988213372763 22.706278580220477 3 23.02463779879677 26.831122003209483 27.49573102454907 22.64850917344468 4 22.453438650042276 26.829391267607193 28.016372291137575 22.700797791212953 5 22.958491338465965 26.62265588782394 27.791374993162176 22.62747778054792 6 22.863614947907987 26.663814750122448 27.94087508126576 22.531695220703803 7 22.813092579698424 26.267165746605674 27.883167295474447 23.036574378221452 8 22.63851375368756 26.800090150093858 27.870597304875215 22.690798791343372 9 22.66482436078944 26.455593067202315 27.96639480806541 22.913187763942837 10-11 23.118766359122 26.582737372317705 27.632771626814435 22.665724641745864 12-13 23.12165942148551 26.352098031101484 27.648743230922644 22.87749931649036 14-15 22.822521245816247 26.578305648359745 27.80991264726626 22.789260458557738 16-17 23.149006223133693 26.370688141689374 27.779472922241265 22.700832712935668 18-19 23.026808507312982 26.37007633249113 27.66570699626694 22.937408163928946 20-21 23.008991992623194 26.707086498852604 27.495863088489763 22.78805842003444 22-23 23.031628855292972 26.7266322887183 27.227277127337224 23.014461728651504 24-25 23.109258792159803 26.802711518634727 27.17726785643831 22.91076183276716 26-27 23.06442934257545 26.830747295471554 27.109906535600807 22.99491682635219 28-29 23.234047884232698 26.830022354949 27.033696017814385 22.902233743003915 30-31 23.093959195258446 26.689209371897167 27.11205839374844 23.104773039095953 32-33 22.978659151788218 27.045899315702915 27.156585721403246 22.81885581110562 34-35 23.120644089876947 26.717219255542695 27.13924846434002 23.022888190240344 36-37 23.11786594929351 26.71434001854267 27.379693203995092 22.788100828168727 38-39 23.02029642992372 26.756753418241274 27.38383223177539 22.839117920059614 40-41 22.89857246210584 26.787636407636057 27.227808629760247 23.085982500497863 42-43 22.8241295629636 27.123681587135966 27.017337588437755 23.03485126146267 44-45 22.81975152093064 26.80522319062352 27.365797920580732 23.009227367865112 46-47 22.83652346322627 26.79150724420297 27.27446671334586 23.0975025792249 48-49 22.98142822841218 26.74590975463885 27.22532602537367 23.047335991575302 50-51 22.828449355308344 26.913473546924145 27.116783715823615 23.141293381943896 52-53 22.95577032341707 26.871236395491948 27.08588799666784 23.087105284423142 54-55 22.950837650796053 26.814330194506425 27.118758587653947 23.11607356704358 56-57 22.840089012748788 27.12020061270316 27.077320214603663 22.96239015994439 58-59 22.923681423237678 27.04715769300919 27.076387902399084 22.952772981354048 60-61 22.93170294280549 26.93521269925784 26.95877158019965 23.174312777737022 62-63 23.009497474172573 26.776455763795237 27.118993612128612 23.095053149903578 64-65 22.95573796074095 26.819146342723933 27.035526657787585 23.18958903874753 66-67 22.913873576437858 26.947873184165715 27.13724028987802 23.001012949518408 68-69 22.951817017796717 26.69169524833877 27.181942308198987 23.17454542566553 70-71 22.816868907094662 26.68581257022091 27.194265560197366 23.303052962487058 72-73 22.976087108679653 26.58575265105011 27.13479762983477 23.30336261043546 74-75 22.898838223165434 26.50788759833405 27.067365802106057 23.525908376394455 76-77 22.72918294729843 26.385003539066705 27.46081171004777 23.425001803587094 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5128.0 1 3214.5 2 1322.0 3 1411.5 4 1480.0 5 1586.0 6 1804.5 7 2000.5 8 2233.0 9 2382.0 10 2438.0 11 2573.5 12 2798.5 13 2944.0 14 3097.5 15 3481.5 16 4018.5 17 4325.0 18 4703.5 19 5944.0 20 8076.0 21 10944.0 22 12542.0 23 14681.0 24 19421.0 25 25955.0 26 29888.0 27 34024.0 28 43424.0 29 52755.5 30 56823.0 31 63303.0 32 76564.5 33 89332.5 34 102099.0 35 108879.0 36 115346.0 37 128754.0 38 138798.0 39 141901.0 40 147296.5 41 159483.0 42 176091.0 43 185908.0 44 186349.5 45 187362.5 46 191113.0 47 195941.0 48 197590.0 49 198547.5 50 197867.0 51 189532.5 52 182836.0 53 180921.0 54 170368.0 55 152239.0 56 133163.5 57 123579.0 58 116776.0 59 98346.5 60 79722.5 61 72725.0 62 63306.5 63 46222.0 64 33755.5 65 28955.0 66 25278.5 67 19565.0 68 15064.0 69 10442.0 70 8284.0 71 7023.0 72 4896.0 73 3663.0 74 3296.0 75 2727.5 76 1781.0 77 1136.5 78 870.0 79 719.0 80 472.5 81 308.5 82 213.0 83 186.0 84 179.5 85 143.5 86 110.0 87 106.0 88 89.0 89 74.0 90 70.0 91 64.0 92 53.5 93 45.0 94 40.5 95 42.5 96 51.0 97 44.5 98 42.0 99 89.5 100 133.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002722150135653815 2 0.008292475876204677 3 0.011543932982680067 4 0.01890382038648483 5 0.013736776147512309 6 0.013333494645933964 7 0.017290694380171456 8 0.018500538884906485 9 0.017240284192474163 10-11 0.020655574408965753 12-13 0.019975036875052303 14-15 0.008015219843869567 16-17 0.0060114148829021755 18-19 0.011720368639620593 20-21 0.01064915215105312 22-23 0.014316493306031176 24-25 0.022495546259916944 26-27 0.0136233532251934 28-29 0.00810343767233983 30-31 0.007939604562323628 32-33 0.009792178960199141 34-35 0.010787780167220675 36-37 0.009729166225577525 38-39 0.016282490626225596 40-41 0.012148855235047582 42-43 0.015337299606901358 44-45 0.018135065024101112 46-47 0.016622759393182324 48-49 0.013724173600587985 50-51 0.02768779559273811 52-53 0.03169540551467289 54-55 0.025255504036343728 56-57 0.015236479231506771 58-59 0.016773989956274202 60-61 0.020038049609673914 62-63 0.025936041570257185 64-65 0.020794202425133308 66-67 0.020617766768192786 68-69 0.029905843851418996 70-71 0.0576440496318544 72-73 0.06930140553685338 74-75 0.08122341492726314 76-77 0.07889194374626333 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3967452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.823153943114654 #Duplication Level Percentage of deduplicated Percentage of total 1 75.48620273052556 39.874193074157134 2 13.163749677252968 13.907015513403184 3 4.626485903182377 7.331567312383575 4 2.1617436441103295 4.567604691935583 5 1.1922824459919483 3.1490059594152986 6 0.7373789093897016 2.3370407787058918 7 0.4797689303548692 1.7740035645681096 8 0.35340409864184374 1.4934335285348628 9 0.25743918384144293 1.2238874675141702 >10 1.3679687113692602 13.139796449705571 >50 0.1087164975021141 3.9473031119851516 >100 0.06140677919608584 5.73467819810404 >500 0.002829122670606803 0.9844759475523801 >1k 5.754147422926859E-4 0.386804523107547 >5k 4.795122852438993E-5 0.14918987892760333 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5890 0.14845800276852752 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2602546924323218E-4 0.0 3 2.5205093848646434E-5 0.0 0.0 1.7643565694052505E-4 0.0 4 2.5205093848646434E-5 0.0 0.0 4.788967831242823E-4 0.0 5 5.041018769729287E-5 0.0 0.0 9.82998660097211E-4 0.0 6 5.041018769729287E-5 2.5205093848646434E-5 0.0 0.0020668176955890077 0.0 7 5.041018769729287E-5 2.5205093848646434E-5 0.0 0.004108430297329369 2.5205093848646434E-5 8 5.041018769729287E-5 2.5205093848646434E-5 0.0 0.0063768887437075485 7.561528154593931E-5 9 5.041018769729287E-5 2.5205093848646434E-5 0.0 0.009351089817847828 7.561528154593931E-5 10 5.041018769729287E-5 2.5205093848646434E-5 0.0 0.012451316361231339 7.561528154593931E-5 11 7.561528154593931E-5 2.5205093848646434E-5 0.0 0.016030439687739133 7.561528154593931E-5 12 7.561528154593931E-5 2.5205093848646434E-5 0.0 0.02039092092355497 7.561528154593931E-5 13 7.561528154593931E-5 2.5205093848646434E-5 0.0 0.026666989291867928 7.561528154593931E-5 14 1.2602546924323218E-4 2.5205093848646434E-5 0.0 0.03503508044961855 7.561528154593931E-5 15 1.2602546924323218E-4 2.5205093848646434E-5 0.0 0.04478945176904472 7.561528154593931E-5 16 1.2602546924323218E-4 2.5205093848646434E-5 0.0 0.05409013139919525 7.561528154593931E-5 17 1.5123056309187863E-4 2.5205093848646434E-5 0.0 0.06341601612319443 7.561528154593931E-5 18 1.7643565694052505E-4 2.5205093848646434E-5 0.0 0.07259067028410174 7.561528154593931E-5 19 2.0164075078917148E-4 2.5205093848646434E-5 0.0 0.08332804026362511 7.561528154593931E-5 20 2.0164075078917148E-4 2.5205093848646434E-5 0.0 0.09310761667689993 7.561528154593931E-5 21 2.0164075078917148E-4 2.5205093848646434E-5 0.0 0.10475237003497459 7.561528154593931E-5 22 2.2684584463781793E-4 2.5205093848646434E-5 0.0 0.11828750543169772 7.561528154593931E-5 23 3.0246112618375725E-4 2.5205093848646434E-5 0.0 0.1315705898899344 7.561528154593931E-5 24 3.528713138810501E-4 2.5205093848646434E-5 0.0 0.1448032641604738 7.561528154593931E-5 25 3.528713138810501E-4 2.5205093848646434E-5 0.0 0.15896852690341307 7.561528154593931E-5 26 3.528713138810501E-4 2.5205093848646434E-5 0.0 0.1748981462157576 7.561528154593931E-5 27 3.528713138810501E-4 2.5205093848646434E-5 0.0 0.18999599743109682 7.561528154593931E-5 28 3.528713138810501E-4 2.5205093848646434E-5 0.0 0.20645492371426297 7.561528154593931E-5 29 3.528713138810501E-4 2.5205093848646434E-5 0.0 0.22261138887124532 1.0082037539458574E-4 30 3.7807640772969655E-4 2.5205093848646434E-5 0.0 0.2391963406236547 1.0082037539458574E-4 31 3.7807640772969655E-4 2.5205093848646434E-5 0.0 0.25885631382559887 1.0082037539458574E-4 32 3.7807640772969655E-4 2.5205093848646434E-5 0.0 0.28113761678780236 1.0082037539458574E-4 33 3.7807640772969655E-4 2.5205093848646434E-5 0.0 0.30420027765931384 1.0082037539458574E-4 34 3.7807640772969655E-4 2.5205093848646434E-5 0.0 0.32869962888019816 1.0082037539458574E-4 35 3.7807640772969655E-4 2.5205093848646434E-5 0.0 0.3548373112012445 1.0082037539458574E-4 36 3.7807640772969655E-4 2.5205093848646434E-5 0.0 0.3835711181887015 1.0082037539458574E-4 37 3.7807640772969655E-4 2.5205093848646434E-5 0.0 0.41245615573925026 1.0082037539458574E-4 38 3.7807640772969655E-4 2.5205093848646434E-5 0.0 0.442273781762199 1.0082037539458574E-4 39 4.0328150157834295E-4 2.5205093848646434E-5 0.0 0.47304920135139633 1.0082037539458574E-4 40 4.0328150157834295E-4 2.5205093848646434E-5 0.0 0.5053873367592097 1.0082037539458574E-4 41 4.0328150157834295E-4 2.5205093848646434E-5 0.0 0.5400947509887958 1.0082037539458574E-4 42 4.5369168927563585E-4 2.5205093848646434E-5 0.0 0.5775747255417331 1.0082037539458574E-4 43 4.5369168927563585E-4 2.5205093848646434E-5 0.0 0.6180289011688106 1.0082037539458574E-4 44 5.545120646702216E-4 2.5205093848646434E-5 0.0 0.6611296116499961 1.0082037539458574E-4 45 5.545120646702216E-4 2.5205093848646434E-5 0.0 0.7073053435807163 1.0082037539458574E-4 46 5.545120646702216E-4 2.5205093848646434E-5 0.0 0.7567073275240633 1.0082037539458574E-4 47 5.797171585188681E-4 2.5205093848646434E-5 0.0 0.8085037953830317 1.0082037539458574E-4 48 5.797171585188681E-4 2.5205093848646434E-5 0.0 0.861736953591373 1.2602546924323218E-4 49 5.797171585188681E-4 2.5205093848646434E-5 0.0 0.9169613142137574 1.2602546924323218E-4 50 5.797171585188681E-4 2.5205093848646434E-5 0.0 0.9783105126413628 1.2602546924323218E-4 51 6.049222523675145E-4 2.5205093848646434E-5 0.0 1.0380717901565035 1.2602546924323218E-4 52 6.049222523675145E-4 2.5205093848646434E-5 0.0 1.1049661092308112 1.2602546924323218E-4 53 6.049222523675145E-4 2.5205093848646434E-5 0.0 1.175414346537778 1.2602546924323218E-4 54 6.049222523675145E-4 2.5205093848646434E-5 0.0 1.2525923439023332 1.2602546924323218E-4 55 6.301273462161608E-4 2.5205093848646434E-5 0.0 1.3303248533315588 1.2602546924323218E-4 56 7.309477216107467E-4 2.5205093848646434E-5 0.0 1.4160473775108053 1.2602546924323218E-4 57 7.309477216107467E-4 2.5205093848646434E-5 0.0 1.502979746194787 1.5123056309187863E-4 58 7.309477216107467E-4 2.5205093848646434E-5 0.0 1.5986582824442488 1.5123056309187863E-4 59 7.561528154593931E-4 2.5205093848646434E-5 0.0 1.6913122074318732 1.5123056309187863E-4 60 7.561528154593931E-4 2.5205093848646434E-5 0.0 1.790343021163205 1.5123056309187863E-4 61 7.561528154593931E-4 2.5205093848646434E-5 0.0 1.8943140332888715 1.5123056309187863E-4 62 7.561528154593931E-4 2.5205093848646434E-5 0.0 2.004561113782851 1.5123056309187863E-4 63 7.813579093080396E-4 2.5205093848646434E-5 0.0 2.1220924663990894 1.5123056309187863E-4 64 7.813579093080396E-4 2.5205093848646434E-5 0.0 2.243379377998776 2.0164075078917148E-4 65 7.813579093080396E-4 2.5205093848646434E-5 0.0 2.376764734645813 2.0164075078917148E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTTCG 255 0.0 27.841595 10 CGCGGAA 505 0.0 25.309677 59 ATTCGCG 505 0.0 25.306807 56 AATTCGC 515 0.0 24.816349 55 CTCGCGC 535 0.0 24.546637 36 CGATCGT 580 0.0 24.478317 1 TCGCGGA 575 0.0 22.845669 58 CGCGCTT 630 0.0 22.536161 38 CGGTTAC 535 0.0 21.894325 4 CAATTCG 600 0.0 21.300968 54 TCGCGCT 630 0.0 20.845686 37 ACGATCG 275 0.0 20.656786 59 CGTTCGG 345 0.0 20.57831 11 CCTCGCG 645 0.0 20.359877 35 CGCTCCG 795 0.0 20.089933 25 GATCGTT 765 0.0 18.558723 2 AACTCGA 775 0.0 18.321335 13 ATCGTTC 775 0.0 18.319717 3 GGTTGCG 430 0.0 18.16064 6 GACGCTC 815 0.0 17.857681 23 >>END_MODULE