FastQCFastQC Report
Thu 26 May 2016
SRR936168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3967452
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83430.2102860979792572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59890.15095330705954352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41190.10381978156257467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13100.044.52875100-101
CGGTATC10050.035.536194100-101
CGTACAT20600.033.806828100-101
GGTATCA84000.033.7210541
GTATCAA167550.032.3199351
ACGTATC5350.028.370838100-101
TCGTATC6400.027.901468100-101
TACGCAG13600.026.916708100-101
TCGTACA11950.026.39938100-101
CGAGTAC13550.025.698177100-101
ATCAACG210050.025.4074543
TATCAAC213400.025.1768362
TCAACGC212300.025.1101554
TCGTATA4650.024.961311100-101
CCGTATA4550.024.855812100-101
CCGTACA12000.024.801302100-101
CAACGCA219350.024.3290025
AGTATCA39950.023.839048100-101
GGGTATC18650.023.777334100-101
CGTATAG4450.023.40797100-101
Produced by FastQC (version 0.11.5)