FastQCFastQC Report
Thu 26 May 2016
SRR936167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227252
Sequences flagged as poor quality0
Sequence length77
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134410.6034790854380195No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57170.25668402138599494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC3050.023.2720854
CGCGGAA3850.023.05006659
CGATCGT4350.022.0297181
ATTCGCG4100.021.64457556
TCGCGCT4500.021.29730637
CGCGCTT4700.019.63449138
GGCGCGA950.006638641518.68772147
TGCGTAA3800.018.68184737
CTCGCGC5050.018.27409636
GCCTAGT4100.018.1822617
TTGCGTA4100.018.1798136
AATTCGC4750.017.93497555
ATACTCG4950.017.92984423
CAATTCG4950.017.92903754
ATCGTTC5350.017.9108073
ACGCTCG1803.1966028E-617.75094445
GACGTAT3800.017.7465639
CCTCGCG5450.017.58453835
AACTCGA5300.017.4121113
TCGCGGA5100.017.40054158
Produced by FastQC (version 0.11.5)