##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936167_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2227252 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.54062045965163 39.0 36.0 41.0 30.0 41.0 2 36.39767345589991 39.0 36.0 41.0 30.0 41.0 3 36.29927058096704 39.0 36.0 41.0 29.0 41.0 4 36.171266206069184 39.0 35.0 41.0 29.0 41.0 5 36.0831374267483 39.0 35.0 41.0 29.0 41.0 6 35.96440142381733 39.0 35.0 40.0 29.0 41.0 7 35.76295407973593 39.0 35.0 40.0 28.0 41.0 8 35.5628465032246 38.0 35.0 40.0 27.0 41.0 9 35.427336915625176 38.0 35.0 40.0 27.0 41.0 10-11 35.61006612632966 39.0 35.0 40.0 27.5 41.0 12-13 35.75822246427436 39.0 35.0 41.0 28.0 41.0 14-15 35.669271371178475 39.0 35.0 41.0 27.0 41.0 16-17 35.45258259954419 39.0 35.0 41.0 26.5 41.0 18-19 35.237479638586024 39.0 35.0 41.0 26.0 41.0 20-21 34.95647304391241 39.0 34.5 41.0 24.5 41.0 22-23 34.57959314886685 38.5 34.0 41.0 23.5 41.0 24-25 34.256038607216425 38.0 33.5 40.0 21.5 41.0 26-27 33.268392171159796 37.5 32.0 39.5 19.0 40.5 28-29 33.26469456532085 37.5 33.0 39.5 19.0 40.5 30-31 33.5924221866228 38.0 33.0 40.0 18.5 41.0 32-33 33.40105654860788 38.0 33.0 40.0 16.0 41.0 34-35 33.10041477120685 37.0 33.0 40.0 13.5 41.0 36-37 32.78767557510331 37.0 32.0 40.0 10.5 41.0 38-39 32.48729286133765 36.0 32.0 40.0 8.0 41.0 40-41 32.07586523662343 36.0 31.5 39.0 7.0 41.0 42-43 31.7030560529298 35.0 31.0 39.0 2.0 41.0 44-45 31.328124073970976 35.0 31.0 38.5 2.0 40.0 46-47 30.926120394099996 35.0 31.0 37.5 2.0 40.0 48-49 30.478528922636507 35.0 30.0 37.0 2.0 39.0 50-51 30.05213913827443 35.0 30.0 36.5 2.0 39.0 52-53 29.61231957587197 34.0 29.0 36.0 2.0 39.0 54-55 29.166764245806043 34.0 29.0 35.5 2.0 37.5 56-57 28.73398048357348 34.0 29.0 35.0 2.0 37.0 58-59 28.317935734259077 34.0 28.0 35.0 2.0 36.5 60-61 27.983994177578467 34.0 27.0 35.0 2.0 36.0 62-63 27.645312025760894 34.0 27.0 35.0 2.0 36.0 64-65 27.269233791236914 33.5 26.0 35.0 2.0 35.0 66-67 26.88416735061861 33.0 25.5 35.0 2.0 35.0 68-69 26.511567842345634 33.0 25.0 35.0 2.0 35.0 70-71 26.081110938501794 33.0 24.0 35.0 2.0 35.0 72-73 25.585077261127164 33.0 21.5 35.0 2.0 35.0 74-75 24.986353587290527 32.5 16.5 35.0 2.0 35.0 76-77 23.31527775033988 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.1560729445988471 1101 2 0.9394278497698494 1101 3 1.090989327515473 1101 4 1.0977126914569268 1101 5 1.272344269150608 1101 6 1.0353456813110071 1101 7 1.2290953953230073 1101 8 1.2321077355122867 1101 9 1.330303392263211 1101 10-11 1.2089454401114423 1101 12-13 1.178809117839286 1101 14-15 1.3281794239013323 1101 16-17 1.42385384708966 1101 18-19 1.3933840525131203 1101 20-21 1.2958559753334455 1101 22-23 1.3331839527124316 1101 24-25 1.4979213572881491 1101 26-27 1.3201018904712 1101 28-29 1.4948768323361818 1101 30-31 1.485773867275796 1101 32-33 1.5475659662179524 1101 34-35 1.459831846501416 1101 36-37 1.5406045371400623 1101 38-39 1.467653457743289 1101 40-41 1.323463411765303 1101 42-43 1.3177523329558234 1101 44-45 1.2892568081698101 1101 46-47 1.271498036723898 1101 48-49 1.344639917062075 1101 50-51 1.2089938648647554 1101 52-53 1.314211715055439 1101 54-55 1.1442944353290976 1101 56-57 1.0256848752347132 1101 58-59 1.0544752112828668 1101 60-61 0.9745143166157 1101 62-63 0.8676868167107123 1101 64-65 0.7602399488817291 1101 66-67 0.7795786906793616 1101 68-69 0.9206826059729067 1101 70-71 0.9727391225505855 1101 72-73 1.0576953428542133 1101 74-75 1.1013545141835976 1101 76-77 0.973066043691059 1102 1 0.9353240196996211 1102 2 1.0556384540191672 1102 3 1.1137898577303247 1102 4 1.1420068674393846 1102 5 1.1323108234307924 1102 6 1.1292498126680783 1102 7 0.9540786019176437 1102 8 0.9951481824500235 1102 9 1.0275686917190967 1102 10-11 0.9481251423426684 1102 12-13 1.146869483704755 1102 14-15 1.1123306580522296 1102 16-17 1.1445568576588059 1102 18-19 1.17167391589647 1102 20-21 1.1896401076757641 1102 22-23 1.267836262860861 1102 24-25 1.3908677922436823 1102 26-27 1.2533617050777437 1102 28-29 1.2079793473175258 1102 30-31 1.3615824198241526 1102 32-33 1.3056516112700578 1102 34-35 1.4400562814861217 1102 36-37 1.3785929538154775 1102 38-39 1.471993198915598 1102 40-41 1.4546613381593687 1102 42-43 1.4883582658046812 1102 44-45 1.4056937557181897 1102 46-47 1.2233896234250992 1102 48-49 1.3275355138539062 1102 50-51 1.3592889242197863 1102 52-53 1.2912912575669644 1102 54-55 1.3157475114816002 1102 56-57 1.250336518190533 1102 58-59 1.303938288628661 1102 60-61 1.4045534322770052 1102 62-63 1.3557109485173662 1102 64-65 1.2293251369427587 1102 66-67 1.1357934224356505 1102 68-69 1.0731345627244764 1102 70-71 1.0550733210023644 1102 72-73 1.1228709735365605 1102 74-75 1.01590184791214 1102 76-77 1.1436628893287555 1103 1 0.46118634269372905 1103 2 0.38263774014966145 1103 3 0.48044264624149946 1103 4 0.4922722633993146 1103 5 0.5800945010089222 1103 6 0.4850156159423733 1103 7 0.5551091975471678 1103 8 0.6734763019694583 1103 9 0.3956984106484285 1103 10-11 0.5159644893849489 1103 12-13 0.5284082574651592 1103 14-15 0.5661213906762512 1103 16-17 0.6588979351930178 1103 18-19 0.6103582524968729 1103 20-21 0.700612643186254 1103 22-23 0.5558942526385522 1103 24-25 0.4969709249996441 1103 26-27 0.32136193416236836 1103 28-29 0.33136173422719395 1103 30-31 0.5991070511737604 1103 32-33 0.7024068082115349 1103 34-35 0.6486110949722672 1103 36-37 0.48910549025752204 1103 38-39 0.5111953047831079 1103 40-41 0.6806714967790839 1103 42-43 0.8167804193332024 1103 44-45 0.7257435079550874 1103 46-47 0.49287334178218956 1103 48-49 0.4995079609624504 1103 50-51 0.5347615657175631 1103 52-53 0.6864884889699994 1103 54-55 0.7193391258437813 1103 56-57 0.6520791772641381 1103 58-59 0.6016064258094751 1103 60-61 0.7150013544985008 1103 62-63 0.5419144460026608 1103 64-65 0.5807967917607115 1103 66-67 0.6352022938089128 1103 68-69 0.5286658869571603 1103 70-71 0.4291913214453267 1103 72-73 0.41387652669987673 1103 74-75 0.5696062698156723 1103 76-77 0.8188646216267372 1104 1 0.23856457453492652 1104 2 0.3258732780803868 1104 3 0.29572639853795124 1104 4 0.11694739894620909 1104 5 0.0943437280555699 1104 6 -0.05575767347824723 1104 7 0.18645245604965055 1104 8 0.35670825518897686 1104 9 0.13030935746027694 1104 10-11 0.10605976428749386 1104 12-13 0.36922607572029875 1104 14-15 0.3405142302156179 1104 16-17 0.4098389218847842 1104 18-19 0.3230366478905182 1104 20-21 0.42530122140016857 1104 22-23 0.42597787722966984 1104 24-25 0.5161841369008897 1104 26-27 0.3093727555093224 1104 28-29 0.31885725333818726 1104 30-31 0.40019618725835926 1104 32-33 0.46810672307464785 1104 34-35 0.3972724293810188 1104 36-37 0.29962689770476203 1104 38-39 0.3121262094745987 1104 40-41 0.25105226032256667 1104 42-43 0.3567163426203841 1104 44-45 0.17313226988545694 1104 46-47 0.11366690722557493 1104 48-49 0.0709557362010571 1104 50-51 0.13010648214898524 1104 52-53 0.13084284887500175 1104 54-55 0.15405117271388136 1104 56-57 0.1660357462474238 1104 58-59 0.198402030057359 1104 60-61 0.05565814080490128 1104 62-63 0.11184291981186689 1104 64-65 0.11339946013011826 1104 66-67 0.15786859080223792 1104 68-69 -9.581936537372826E-5 1104 70-71 -0.26108262890642564 1104 72-73 -0.1192414439054943 1104 74-75 -0.16954997878404754 1104 76-77 0.0717309903142862 1105 1 0.10122311166204412 1105 2 0.2743487117668906 1105 3 0.19791232288437044 1105 4 0.413239684768385 1105 5 0.2851491299791178 1105 6 0.26927259017649163 1105 7 0.16583568831903506 1105 8 0.33499200055225486 1105 9 0.016350038475799522 1105 10-11 0.24235657577546732 1105 12-13 0.4577673953267052 1105 14-15 0.06420150948528658 1105 16-17 0.11428795677960579 1105 18-19 0.2892253431660592 1105 20-21 0.3944350700485799 1105 22-23 0.30134367874452295 1105 24-25 0.2343233108419298 1105 26-27 0.018290538321785732 1105 28-29 0.14127762383196085 1105 30-31 0.10415864366759564 1105 32-33 0.22754676216751335 1105 34-35 0.18103445154845588 1105 36-37 0.2995762269066873 1105 38-39 0.28629937310935105 1105 40-41 0.32243422651612264 1105 42-43 0.15231450792102308 1105 44-45 0.018907019446771756 1105 46-47 -0.2870821876412677 1105 48-49 -0.11281406011621797 1105 50-51 -0.015710192291830793 1105 52-53 -0.0526333344616603 1105 54-55 -0.14545003227934572 1105 56-57 -0.04775823322882289 1105 58-59 -0.00378000721413585 1105 60-61 -0.18169081856961355 1105 62-63 -0.35815649712689535 1105 64-65 -0.2738774495342611 1105 66-67 -0.28680098006469024 1105 68-69 -0.654812507133304 1105 70-71 -0.9524523508139815 1105 72-73 -0.966718871106135 1105 74-75 -0.8525867838305814 1105 76-77 -0.8208795414375629 1106 1 -0.007863163551050434 1106 2 0.17530979959729365 1106 3 -0.16972761576590756 1106 4 0.13546380838779726 1106 5 -0.10118344446856753 1106 6 -0.04211238297502007 1106 7 0.20976868734111065 1106 8 0.1481441322542807 1106 9 0.18284489489217037 1106 10-11 -0.06753857661999518 1106 12-13 -0.09323195622079794 1106 14-15 -0.008289182414898733 1106 16-17 0.0978604554573721 1106 18-19 -0.01268918887803494 1106 20-21 -0.09843469048033171 1106 22-23 -0.08425522258254858 1106 24-25 0.10032523383900127 1106 26-27 0.03780469179009316 1106 28-29 0.022525025572697643 1106 30-31 0.008960705938193314 1106 32-33 -0.0024179494217193565 1106 34-35 -0.02496466954971055 1106 36-37 0.0452221033922271 1106 38-39 -0.11297110642150443 1106 40-41 -0.125030853563878 1106 42-43 -0.1031021924003781 1106 44-45 -0.24128853849389387 1106 46-47 -0.001986692629238007 1106 48-49 -0.2855676696145615 1106 50-51 -0.26196014266536594 1106 52-53 -0.35382468909763887 1106 54-55 -0.11913739512205623 1106 56-57 -0.33192337459465904 1106 58-59 -0.43169362841291914 1106 60-61 -0.4115699672851889 1106 62-63 -0.5156643997139057 1106 64-65 -0.4981752749595323 1106 66-67 -0.46064557324402955 1106 68-69 -0.5960876266817507 1106 70-71 -0.8775919095651012 1106 72-73 -0.8498181956842181 1106 74-75 -1.0132686126947945 1106 76-77 -0.7448825970589077 1107 1 -0.32854697818004297 1107 2 -0.330510657684151 1107 3 -0.263335804945946 1107 4 -0.2789873093267019 1107 5 -0.3355531514760912 1107 6 -0.5169901510549124 1107 7 -0.5725363457119883 1107 8 -0.28433651091016543 1107 9 -0.32990176200550536 1107 10-11 -0.2854266429667476 1107 12-13 -0.2998969751059164 1107 14-15 -0.16197202934003485 1107 16-17 -0.1329748704778666 1107 18-19 -0.13114176752741713 1107 20-21 -0.16287438774460838 1107 22-23 -0.2276847120608423 1107 24-25 -0.3742142240923627 1107 26-27 -0.3964262043345599 1107 28-29 -0.17373745278637642 1107 30-31 0.06960859304456335 1107 32-33 0.14969273736112854 1107 34-35 0.1293427160057945 1107 36-37 0.05167129231957546 1107 38-39 -0.004559255194109824 1107 40-41 0.13659279239553967 1107 42-43 0.25725119053559453 1107 44-45 0.14354466224451556 1107 46-47 0.08356638267180472 1107 48-49 0.16995944209288893 1107 50-51 0.054950951060668274 1107 52-53 0.08498277314591007 1107 54-55 0.051715409067426776 1107 56-57 0.10318518588793424 1107 58-59 0.018967268075872568 1107 60-61 0.04543672719514902 1107 62-63 -0.022984742790850277 1107 64-65 0.11380372906686631 1107 66-67 0.17131822558391008 1107 68-69 0.04532910499504439 1107 70-71 -0.07438590781506704 1107 72-73 -0.24953229822992995 1107 74-75 -0.22865984863026156 1107 76-77 -0.2457635716922688 1108 1 -0.03118023264921277 1108 2 0.055322439544589486 1108 3 -0.09539006125829985 1108 4 -0.11674466768747038 1108 5 -0.014389572290795627 1108 6 -0.08165266437173813 1108 7 -0.16483574654649402 1108 8 -0.00527614793986686 1108 9 -0.14683668182229326 1108 10-11 -0.11336379279575937 1108 12-13 -0.20077089316315977 1108 14-15 -0.1018704294395647 1108 16-17 -0.012005646319295238 1108 18-19 -0.06984288211534562 1108 20-21 -0.3344979265534036 1108 22-23 -0.2666836843524365 1108 24-25 -0.20199758358016595 1108 26-27 -0.3895678451342732 1108 28-29 -0.17938483066628663 1108 30-31 -0.19227973488536776 1108 32-33 -0.23087050538820364 1108 34-35 -0.1037285847928544 1108 36-37 -0.10342705090575066 1108 38-39 -0.08585632146823485 1108 40-41 -0.1021436594699594 1108 42-43 -0.22114738259113764 1108 44-45 -0.20188527637525766 1108 46-47 -0.2941261767403027 1108 48-49 -0.31859493915451864 1108 50-51 -0.3370629287528608 1108 52-53 -0.30331096954225245 1108 54-55 -0.26329530189141437 1108 56-57 -0.3170526193534826 1108 58-59 -0.30723448686951826 1108 60-61 -0.4814818099251994 1108 62-63 -0.4082404205399719 1108 64-65 -0.3137755524154606 1108 66-67 -0.41037528730739936 1108 68-69 -0.25883473451516537 1108 70-71 -0.3381625845845697 1108 72-73 -0.19981442143575023 1108 74-75 -0.43279828293794864 1108 76-77 -0.3263501286809465 1109 1 0.18891207520319853 1109 2 0.11083785974305016 1109 3 -0.015834013116688084 1109 4 0.24320642059817743 1109 5 0.06083664438810388 1109 6 0.039400706634026506 1109 7 -0.07650597752425625 1109 8 0.003442417177751622 1109 9 0.19732820268882278 1109 10-11 0.10946226361922129 1109 12-13 0.16975501183364372 1109 14-15 0.11089781749174676 1109 16-17 0.3207263851299018 1109 18-19 0.2004788424908881 1109 20-21 0.24647604096713138 1109 22-23 0.2416832367592079 1109 24-25 0.3180456044229345 1109 26-27 0.26045468547457773 1109 28-29 0.3474772747228414 1109 30-31 0.39229288600201073 1109 32-33 0.47357317589474945 1109 34-35 0.5096087031046181 1109 36-37 0.5103429495625491 1109 38-39 0.45504521508807017 1109 40-41 0.4578813167245954 1109 42-43 0.451422303593386 1109 44-45 0.37257760535725737 1109 46-47 0.2144835337960096 1109 48-49 0.394513608427733 1109 50-51 0.428884215375394 1109 52-53 0.5459293899068136 1109 54-55 0.4438095929223813 1109 56-57 0.5710320707595891 1109 58-59 0.6235670928747865 1109 60-61 0.6903099718661139 1109 62-63 0.5157686749575454 1109 64-65 0.5359539241771643 1109 66-67 0.6039555327963555 1109 68-69 0.5264265008698565 1109 70-71 0.6244142302221505 1109 72-73 0.5712019535818094 1109 74-75 0.4671247071288107 1109 76-77 0.6083204717363664 1110 1 -0.6531011575491235 1110 2 -0.8799963680356555 1110 3 -0.8766325134719466 1110 4 -0.8774319362426937 1110 5 -0.5846042037980652 1110 6 -0.8266169202386422 1110 7 -0.8709816813645759 1110 8 -0.7134192133835526 1110 9 -0.7563761113093719 1110 10-11 -0.6852306266739063 1110 12-13 -0.6129528144373566 1110 14-15 -0.7250556612708436 1110 16-17 -0.872745725811555 1110 18-19 -0.6760834638175908 1110 20-21 -0.9612034905954445 1110 22-23 -1.126346111127127 1110 24-25 -0.838609018725812 1110 26-27 -0.7748545910576965 1110 28-29 -0.7010291778816296 1110 30-31 -0.6991661937522764 1110 32-33 -0.4738480499237383 1110 34-35 -0.3966937353298192 1110 36-37 -0.5174273871016482 1110 38-39 -0.4031472493986641 1110 40-41 -0.5053583392036813 1110 42-43 -0.460444038553387 1110 44-45 -0.4612772778105416 1110 46-47 -0.6598620597377796 1110 48-49 -0.5029026286531995 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12-13 -0.7700196732169289 2314 14-15 -0.7737330816123418 2314 16-17 -1.0665301703882903 2314 18-19 -0.6853170030222131 2314 20-21 -0.6529370881772181 2314 22-23 -0.62027587370806 2314 24-25 -0.4761186262899173 2314 26-27 -0.43417250580600353 2314 28-29 -0.40161974091036967 2314 30-31 -0.445319879258264 2314 32-33 -0.5386449648473359 2314 34-35 -0.5120173930746006 2314 36-37 -0.6851427322027206 2314 38-39 -0.5362733904253716 2314 40-41 -0.34976982019756164 2314 42-43 -0.30873126928259254 2314 44-45 -0.2516926863536 2314 46-47 -0.32803726881843787 2314 48-49 -0.4526751715258719 2314 50-51 -0.30098566588802456 2314 52-53 -0.30053843394570023 2314 54-55 -0.2427433384138986 2314 56-57 -0.2171616155930387 2314 58-59 -0.3143667871469411 2314 60-61 -0.2820669154591613 2314 62-63 -0.27307362182354566 2314 64-65 -0.15142455837450797 2314 66-67 -0.2605250701129904 2314 68-69 -0.11137363882023621 2314 70-71 -0.006323626655863279 2314 72-73 -0.030117600444629744 2314 74-75 0.16772183917044003 2314 76-77 0.22329231814624606 2315 1 -1.4236739097965767 2315 2 -1.4414552215155538 2315 3 -1.4992636496497695 2315 4 -1.4566773054924482 2315 5 -1.411892242133888 2315 6 -1.2417469434791428 2315 7 -1.449251728201041 2315 8 -1.2762034355715457 2315 9 -1.3801643637692607 2315 10-11 -1.1826106960399372 2315 12-13 -1.2751071902699422 2315 14-15 -1.6567957874432437 2315 16-17 -1.4766969495348121 2315 18-19 -1.316400401284902 2315 20-21 -1.518345332130103 2315 22-23 -1.542665496210212 2315 24-25 -1.513493027805346 2315 26-27 -1.6263770723916657 2315 28-29 -1.536368196364009 2315 30-31 -1.6342024763554193 2315 32-33 -1.5767752020398405 2315 34-35 -1.515893893087629 2315 36-37 -1.7293281994189442 2315 38-39 -1.675336003569008 2315 40-41 -1.712882895763748 2315 42-43 -1.9383309471943484 2315 44-45 -1.8201685891130737 2315 46-47 -1.6031501590100987 2315 48-49 -1.4682738588424904 2315 50-51 -1.397961743884359 2315 52-53 -1.4533365755660483 2315 54-55 -1.6185718032521201 2315 56-57 -1.7544030688134846 2315 58-59 -1.5904163980052566 2315 60-61 -1.565095877332837 2315 62-63 -1.6493287377260692 2315 64-65 -1.6573319232466162 2315 66-67 -1.515202590844524 2315 68-69 -1.6615471377934377 2315 70-71 -1.6085219078864021 2315 72-73 -3.120334459678741 2315 74-75 -3.485480360993499 2315 76-77 -3.109125052605709 2316 1 -3.3904564683731166 2316 2 -3.3905036214151067 2316 3 -2.821002062003636 2316 4 -2.6841731807403946 2316 5 -3.3617153707435747 2316 6 -2.8692854566134685 2316 7 -3.3053550972681833 2316 8 -2.9712935059999026 2316 9 -2.7441587753411483 2316 10-11 -2.6881195783711718 2316 12-13 -2.7089267865076962 2316 14-15 -2.9273154809223385 2316 16-17 -4.1644533035067965 2316 18-19 -3.963414653798935 2316 20-21 -3.382058805533667 2316 22-23 -3.4363147262845857 2316 24-25 -3.4788058432709335 2316 26-27 -3.142597838896151 2316 28-29 -3.110930989499476 2316 30-31 -3.5308876756159115 2316 32-33 -3.7712398606249664 2316 34-35 -3.633996709379012 2316 36-37 -3.5314815812100377 2316 38-39 -3.564375707362096 2316 40-41 -3.473615846086396 2316 42-43 -3.7906495718612945 2316 44-45 -3.441113650280748 2316 46-47 -3.1589109082234863 2316 48-49 -3.3550920950496383 2316 50-51 -3.7407098483522354 2316 52-53 -4.986496042946381 2316 54-55 -4.006319899293537 2316 56-57 -3.8348576426115777 2316 58-59 -4.108855430040972 2316 60-61 -3.850175987685752 2316 62-63 -3.8804978753116224 2316 64-65 -3.8357702137538716 2316 66-67 -3.7696571545308686 2316 68-69 -3.6329886017054385 2316 70-71 -3.3566566267544253 2316 72-73 -3.371172555366247 2316 74-75 -3.4499275262481746 2316 76-77 -3.5773771857639964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57138.0 3 5800.0 4 7844.0 5 9211.0 6 10591.0 7 11067.0 8 13388.0 9 14540.0 10 18752.0 11 21418.0 12 15651.0 13 12138.0 14 11838.0 15 12141.0 16 11992.0 17 12569.0 18 13245.0 19 13890.0 20 14891.0 21 15902.0 22 17742.0 23 20306.0 24 23428.0 25 27308.0 26 32212.0 27 37521.0 28 43961.0 29 52021.0 30 61611.0 31 74066.0 32 90225.0 33 113509.0 34 153362.0 35 204780.0 36 277730.0 37 346095.0 38 278619.0 39 68230.0 40 520.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.91444682275509 25.669186119346893 29.11362053567219 22.30274652222583 2 22.54805064991807 26.09618527031985 28.928280675158614 22.427483404603468 3 22.701667240689492 26.021015971006385 28.550278786496293 22.727038001807834 4 22.460771212104884 26.178839445767636 28.979683295792963 22.380706046334513 5 22.68539390423298 26.11217482985988 28.701860988923155 22.500570276983982 6 22.566014251094607 25.954926164889358 28.955702525799744 22.52335705821629 7 22.551100641286723 25.808815638037853 28.752465839728163 22.887617880947257 8 22.5066752406724 26.171927974759374 28.299607613501543 23.021789171066683 9 22.295214034306202 26.056160935045618 28.88953453387376 22.75909049677442 10-11 22.985708351576797 26.01183299091756 28.33635332816903 22.66610532933661 12-13 23.183614286217864 25.63853455598113 28.250398364913266 22.92745279288774 14-15 22.75647300340892 25.856878194743288 28.471157058339646 22.915491743508138 16-17 22.86002549925947 25.804047238511917 28.67126587277266 22.66466138945595 18-19 22.942997935897182 25.925921767965924 28.103614234383556 23.02746606175334 20-21 22.735152049693458 26.157056043456627 28.102922077338853 23.00486982951106 22-23 23.118626150658102 26.309045148840937 27.490167047365897 23.08216165313506 24-25 23.177543569094546 26.50210287104599 27.03734647783975 23.283007082019715 26-27 23.049990165074068 26.645598668557607 27.066165250108003 23.238245916260322 28-29 23.418100341926777 26.477480652961155 27.00891524122339 23.09550376388867 30-31 22.968303470900263 26.453392759601336 27.109433074391127 23.468870695107277 32-33 22.98656305490066 26.835722799610153 26.954333605070246 23.22338054041894 34-35 23.119615931488056 26.31665445665994 26.98257318089797 23.58115643095404 36-37 22.942744507081528 26.411113653115486 27.491882112763367 23.15425972703962 38-39 23.00544577074327 26.41480585058314 27.533776464077107 23.045971914596482 40-41 22.853539067107825 26.5564910605897 27.26178317622132 23.328186696081154 42-43 22.835440173136494 26.795472747369846 27.113087239356176 23.25599984013748 44-45 22.92276663440293 26.40147986592476 27.421099225692803 23.25465427397951 46-47 22.768323675643533 26.535765786763776 27.237424681920473 23.458485855672215 48-49 22.879321916527097 26.65168666482691 27.00552128407971 23.463470134566283 50-51 22.74451434206127 26.944943668579153 26.935309759374594 23.375232229984995 52-53 22.8652577132519 26.68491862301408 27.110128870100446 23.33969479363357 54-55 22.806230828995766 26.726681546883913 27.10653127764779 23.360556346472535 56-57 22.81079248392612 26.939872980731554 26.977347818813985 23.271986716528335 58-59 22.79570650553414 26.84226621354131 26.933491520065527 23.428535760859024 60-61 22.75558667120528 26.713633534487325 26.868450856783088 23.662328937524308 62-63 23.008930433960572 26.517310392477878 26.837356343648473 23.63640282991308 64-65 22.81068872879019 26.496396881099322 26.775242266295773 23.917672123814715 66-67 22.75478549077595 26.617152878592414 26.91636885414316 23.711692776488473 68-69 22.877731965384605 26.29040216264241 27.064089863658037 23.767776008314954 70-71 22.73511775878272 26.06636071724415 27.154418806905067 24.04410271706806 72-73 23.0280031140784 25.868038119375253 26.982231104610342 24.12172766193601 74-75 22.79162304135455 26.080102813439442 26.86068509339936 24.26758905180665 76-77 22.76068847344885 25.765446096228278 27.32417759454916 24.14968783577371 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14577.0 1 8636.0 2 2552.0 3 2381.5 4 2354.0 5 2472.5 6 2612.0 7 2716.0 8 2856.0 9 2913.0 10 3028.0 11 3109.5 12 3264.5 13 3453.0 14 3691.5 15 4082.0 16 4566.5 17 4899.0 18 5339.0 19 6143.5 20 7031.0 21 8494.0 22 9434.0 23 10611.0 24 13054.0 25 15988.5 26 17657.0 27 18705.0 28 22315.0 29 26101.5 30 27326.0 31 30667.5 32 37000.5 33 42617.0 34 48935.5 35 52629.0 36 56049.5 37 62668.5 38 67126.5 39 68386.0 40 71914.0 41 79105.0 42 88078.5 43 93389.0 44 92697.5 45 93650.5 46 98263.5 47 102609.5 48 103987.0 49 104736.0 50 106645.0 51 106468.0 52 105131.0 53 104921.0 54 101277.5 55 91941.0 56 82307.5 57 78577.0 58 75712.0 59 66174.5 60 55072.0 61 50642.0 62 44906.0 63 33667.0 64 24722.0 65 21280.0 66 18483.0 67 14266.5 68 11114.5 69 7819.5 70 6257.0 71 5258.0 72 3652.5 73 2790.0 74 2534.0 75 2222.5 76 1512.0 77 924.0 78 735.0 79 585.5 80 383.5 81 266.0 82 172.0 83 143.0 84 125.0 85 102.0 86 82.5 87 68.0 88 63.0 89 54.0 90 44.0 91 38.0 92 40.0 93 42.0 94 39.0 95 34.5 96 33.0 97 33.5 98 41.0 99 77.0 100 106.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008126606239437657 2 0.012526647186757493 3 0.012526647186757493 4 0.0141878871362558 5 0.011853171531555478 6 0.008755183517626206 7 0.014502175775350071 8 0.016432805986929182 9 0.016432805986929182 10-11 0.019171606984750716 12-13 0.015108303865031886 14-15 0.007004146814100965 16-17 0.005769441446230601 18-19 0.016904238945570596 20-21 0.021551220966464505 22-23 0.019238954550270918 24-25 0.01865527564909584 26-27 0.022179798244653052 28-29 0.007498028961249108 30-31 0.008014360296903987 32-33 0.009900092131469633 34-35 0.011381738572914066 36-37 0.01012458401653697 38-39 0.013222572030466244 40-41 0.010393974278617777 42-43 0.015198100619058822 44-45 0.013177673653452774 46-47 0.023751241440124422 48-49 0.022696129580307933 50-51 0.03329214655548631 52-53 0.0369962626590974 54-55 0.026130855421838208 56-57 0.019059361042217046 58-59 0.015624635200686765 60-61 0.01948589562384499 62-63 0.024267572775779303 64-65 0.0255920748976766 66-67 0.0269165770195739 68-69 0.04047588687764114 70-71 0.052800491367838036 72-73 0.08416200771174523 74-75 0.08360077799907689 76-77 0.08894368486367954 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2227252.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.633961605615646 #Duplication Level Percentage of deduplicated Percentage of total 1 73.87831314995715 35.929950452226656 2 12.839121182284833 12.48834653258174 3 5.0450069024217745 7.360760159773395 4 2.58502186027173 5.028794156085298 5 1.4746869628977834 3.585993456693638 6 0.9251413583737119 2.6995973581748527 7 0.6265551700342123 2.1330302058270707 8 0.4441219126259989 1.7279526437492334 9 0.3279531139743264 1.4354693238122536 >10 1.6474861631798616 14.462890387964473 >50 0.1292698600443016 4.32772936910477 >100 0.07259104872979301 6.123721176182492 >500 0.0035252936817899393 1.1767266593655328 >1k 0.0010204797499918114 0.6546644617688631 >5k 9.277088636289196E-5 0.25794050502636734 >10k+ 9.277088636289196E-5 0.6064331516633554 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13441 0.6034790854380195 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5717 0.25668402138599494 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.4898377013467716E-5 0.0 2 0.0 0.0 0.0 8.081707862424189E-4 0.0 3 0.0 0.0 0.0 0.0011673578023501606 0.0 4 1.3469513104040316E-4 0.0 0.0 0.0024694107357407244 0.0 5 2.244918850673386E-4 0.0 0.0 0.004804126340441046 0.0 6 2.244918850673386E-4 0.0 0.0 0.0084857932555454 0.0 7 2.244918850673386E-4 0.0 0.0 0.01652260274095612 0.0 8 2.244918850673386E-4 0.0 0.0 0.024873700865461115 0.0 9 2.693902620808063E-4 0.0 0.0 0.034122766530235465 0.0 10 2.693902620808063E-4 0.0 0.0 0.045302462406588924 0.0 11 3.5918701610774173E-4 0.0 0.0 0.05782910959334642 0.0 12 3.5918701610774173E-4 0.0 0.0 0.07381293181014092 0.0 13 4.0408539312120946E-4 0.0 0.0 0.0968009008410364 0.0 14 4.938821471481449E-4 0.0 0.0 0.12800527286539645 0.0 15 4.938821471481449E-4 0.0 0.0 0.16176885237952418 0.0 16 4.938821471481449E-4 0.0 0.0 0.1976426556132849 0.0 17 4.938821471481449E-4 0.0 0.0 0.23095725135727793 0.0 18 4.938821471481449E-4 0.0 0.0 0.2651698146415403 0.0 19 5.836789011750803E-4 0.0 0.0 0.3009987194982876 0.0 20 7.632724092289512E-4 0.0 0.0 0.3401950026310449 0.0 21 7.632724092289512E-4 0.0 0.0 0.3867995179710244 0.0 22 8.979675402693544E-4 0.0 0.0 0.4358285456697311 0.0 23 9.877642942962897E-4 0.0 0.0 0.4871922889731382 0.0 24 0.0010326626713097575 0.0 0.0 0.5379274549983567 0.0 25 0.0010326626713097575 0.0 0.0 0.5920748976765988 0.0 26 0.0010326626713097575 0.0 0.0 0.6495897186308509 0.0 27 0.0010326626713097575 0.0 0.0 0.7101127308450054 0.0 28 0.0010326626713097575 0.0 0.0 0.7676724501762711 0.0 29 0.0010326626713097575 0.0 0.0 0.8244239987212942 0.0 30 0.0010326626713097575 0.0 0.0 0.8854857914596104 0.0 31 0.0010775610483232253 0.0 0.0 0.9541803082902159 0.0 32 0.0010775610483232253 0.0 0.0 1.0276789514612625 0.0 33 0.0010775610483232253 0.0 0.0 1.106610298250939 0.0 34 0.0010775610483232253 0.0 0.0 1.1900763811189754 0.0 35 0.0010775610483232253 0.0 0.0 1.2768200455089949 0.0 36 0.0010775610483232253 0.0 0.0 1.3740250317431526 0.0 37 0.0010775610483232253 0.0 0.0 1.465168737080492 0.0 38 0.0010775610483232253 0.0 0.0 1.5567165278109527 0.0 39 0.0011224594253366929 0.0 0.0 1.6519010870795043 0.0 40 0.0011224594253366929 0.0 0.0 1.753057130490847 0.0 41 0.0011673578023501606 0.0 0.0 1.8612622190933041 0.0 42 0.001257154556377096 0.0 0.0 1.9779082025742933 0.0 43 0.001257154556377096 0.0 0.0 2.105554288423582 0.0 44 0.0013020529333905638 0.0 0.0 2.2382289924983794 0.0 45 0.0013020529333905638 0.0 0.0 2.372924123538782 0.0 46 0.0013020529333905638 0.0 0.0 2.5220316336005086 0.0 47 0.0013020529333905638 0.0 0.0 2.6705554647610596 0.0 48 0.0013469513104040316 0.0 0.0 2.820426247232015 0.0 49 0.0013469513104040316 0.0 0.0 2.9708807086041453 0.0 50 0.0013469513104040316 0.0 0.0 3.1270821622339997 0.0 51 0.0013469513104040316 0.0 0.0 3.291095933464197 0.0 52 0.0013469513104040316 0.0 0.0 3.463775091457994 0.0 53 0.0013469513104040316 0.0 0.0 3.651180917112208 0.0 54 0.0013469513104040316 0.0 0.0 3.847566418169116 0.0 55 0.0013918496874174991 0.0 0.0 4.05140504981026 0.0 56 0.0015265448184579025 0.0 0.0 4.276794902417867 0.0 57 0.00157144319547137 0.0 0.0 4.487323392234018 0.0 58 0.00157144319547137 0.0 0.0 4.719537798147672 0.0 59 0.00157144319547137 0.0 0.0 4.945107244263334 0.0 60 0.00157144319547137 0.0 0.0 5.183697219712902 0.0 61 0.0016163415724848378 0.0 0.0 5.427046423125897 0.0 62 0.0016163415724848378 0.0 0.0 5.66999154114577 0.0 63 0.0016163415724848378 0.0 0.0 5.928740887874385 0.0 64 0.0016163415724848378 0.0 0.0 6.195706637596464 0.0 65 0.0016612399494983056 0.0 0.0 6.4875011898069905 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 305 0.0 23.272085 4 CGCGGAA 385 0.0 23.050066 59 CGATCGT 435 0.0 22.029718 1 ATTCGCG 410 0.0 21.644575 56 TCGCGCT 450 0.0 21.297306 37 CGCGCTT 470 0.0 19.634491 38 GGCGCGA 95 0.0066386415 18.687721 47 TGCGTAA 380 0.0 18.681847 37 CTCGCGC 505 0.0 18.274096 36 GCCTAGT 410 0.0 18.18226 17 TTGCGTA 410 0.0 18.17981 36 AATTCGC 475 0.0 17.934975 55 ATACTCG 495 0.0 17.929844 23 CAATTCG 495 0.0 17.929037 54 ATCGTTC 535 0.0 17.910807 3 ACGCTCG 180 3.1966028E-6 17.750944 45 GACGTAT 380 0.0 17.74656 39 CCTCGCG 545 0.0 17.584538 35 AACTCGA 530 0.0 17.41211 13 TCGCGGA 510 0.0 17.400541 58 >>END_MODULE