FastQCFastQC Report
Thu 26 May 2016
SRR936167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227252
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112380.5045679608773502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83250.3737789886371187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59400.26669635945999826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13000.051.73353100-101
CGTACAT14550.041.9274100-101
GGTATCA86200.041.0005261
CGGTATC11000.038.415165100-101
ACGTATC3800.038.372448100-101
AGTATCA27700.037.063496100-101
TCGTACA8050.035.85774100-101
CCGGTAT3950.035.40852100-101
GCGTATC3900.033.573383100-101
TCGTATC6000.032.73405100-101
AGGTATC22050.032.389908100-101
AACGTAC1750.032.3089398-99
GTATCAA171700.032.192571
CCGTACA6300.031.647638100-101
TACGCAG9900.030.65999100-101
TCGAGTA4950.030.65999100-101
TATACGT2950.030.26056196-97
ATACACG6400.030.22115996-97
CCTGTCG8600.030.10222296-97
CCGTATC6450.029.98891100-101
Produced by FastQC (version 0.11.5)