FastQCFastQC Report
Thu 26 May 2016
SRR936166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233296
Sequences flagged as poor quality0
Sequence length77
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132810.5946815827369054No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56630.2535714029846469No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG3500.027.38511856
AATTCGC3600.026.62442255
CGCGGAA3800.026.15908459
CTCGCGC4350.025.296636
TCGCGCT4100.025.10752337
CGATCGT4100.024.2401181
TCGCGGA4200.022.82297758
CAATTCG4250.022.5524554
CCTCGCG5100.022.2715335
ACTCGAG4650.022.13781414
CGCTCCG4850.021.2253925
AACTCGA5050.021.08770413
ATCGTTC5100.020.8790913
CGCGCTT4950.020.79706238
CGGTTAC3500.020.2830034
GACGTAT3350.020.1343439
AACGCGA900.00486146719.72311853
TCGCGAT900.004865247319.72046926
CGTAAGT4750.019.43159139
GATCGTT5350.018.576542
Produced by FastQC (version 0.11.5)