##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936166_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2233296 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.519560774747276 39.0 36.0 41.0 30.0 41.0 2 36.384481949549006 39.0 36.0 41.0 30.0 41.0 3 36.24149821608958 39.0 36.0 41.0 29.0 41.0 4 36.12621031873966 39.0 35.0 41.0 29.0 41.0 5 36.0365110580953 39.0 35.0 41.0 29.0 41.0 6 35.92941508872984 39.0 35.0 40.0 28.0 41.0 7 35.74483991374184 39.0 35.0 40.0 28.0 41.0 8 35.59316051253394 38.0 35.0 40.0 27.0 41.0 9 35.70846139517556 39.0 35.0 40.0 28.0 41.0 10-11 35.909624384765834 39.0 35.0 41.0 29.0 41.0 12-13 35.79134069106827 39.0 35.0 41.0 27.5 41.0 14-15 35.606671036888976 39.0 35.0 41.0 27.0 41.0 16-17 35.403567417843405 39.0 35.0 41.0 26.0 41.0 18-19 35.187491268510755 39.0 35.0 41.0 25.5 41.0 20-21 34.90973364032354 39.0 34.5 41.0 24.5 41.0 22-23 34.52067885313904 38.5 34.0 40.5 23.0 41.0 24-25 34.19979371296953 38.0 33.5 40.0 21.5 41.0 26-27 33.18747559660699 37.5 32.0 39.5 18.0 40.5 28-29 33.16308832326749 37.5 32.5 39.5 17.5 40.5 30-31 33.52746702631447 38.0 33.0 40.0 18.0 41.0 32-33 33.37110374083865 38.0 33.0 40.0 15.0 41.0 34-35 33.09280744693046 37.0 33.0 40.0 13.0 41.0 36-37 32.76183788445419 37.0 32.0 40.0 9.0 41.0 38-39 32.43009659265946 36.0 32.0 40.0 7.5 41.0 40-41 32.04072724797788 36.0 31.5 39.0 6.0 41.0 42-43 31.65764121728602 35.0 31.0 39.0 2.0 41.0 44-45 31.28624597903726 35.0 31.0 38.5 2.0 40.0 46-47 30.858140837578183 35.0 30.5 37.5 2.0 40.0 48-49 30.449480498778485 35.0 30.0 37.0 2.0 39.0 50-51 30.0091197046876 35.0 30.0 36.5 2.0 39.0 52-53 29.537529284071613 34.0 29.0 36.0 2.0 39.0 54-55 29.091615486706644 34.0 29.0 35.5 2.0 37.5 56-57 28.673349614202507 34.0 29.0 35.0 2.0 37.0 58-59 28.26298350061971 34.0 28.0 35.0 2.0 36.5 60-61 27.92295132396243 34.0 27.0 35.0 2.0 36.0 62-63 27.55489845501895 34.0 26.5 35.0 2.0 36.0 64-65 27.17230161160903 33.5 26.0 35.0 2.0 35.0 66-67 26.813882038028098 33.0 25.0 35.0 2.0 35.0 68-69 26.44343203946096 33.0 25.0 35.0 2.0 35.0 70-71 25.98204425208302 33.0 23.5 35.0 2.0 35.0 72-73 25.494864764903532 33.0 21.5 35.0 2.0 35.0 74-75 24.913026531189775 32.5 11.5 35.0 2.0 35.0 76-77 23.190989237432028 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.109727100142024 1101 2 0.9468520467403749 1101 3 1.226593129596104 1101 4 1.148906338093461 1101 5 0.9960929061559156 1101 6 1.1172297797315096 1101 7 1.1922631748529184 1101 8 1.1369564511380545 1101 9 1.1639325089273385 1101 10-11 1.1844529305066658 1101 12-13 1.3466513121128187 1101 14-15 1.353081499380103 1101 16-17 1.2952974767621654 1101 18-19 1.3353063050553544 1101 20-21 1.2423753177130692 1101 22-23 1.1641114848571519 1101 24-25 1.2034370110869688 1101 26-27 1.182833421887068 1101 28-29 1.288619524108448 1101 30-31 1.080275384746976 1101 32-33 1.1385367680367438 1101 34-35 1.1816929093029245 1101 36-37 1.1423041738142956 1101 38-39 1.1463993092624634 1101 40-41 1.0604919019432728 1101 42-43 1.0671661157534977 1101 44-45 0.952166036048606 1101 46-47 1.0538852131111973 1101 48-49 0.9954431013920058 1101 50-51 0.9290826503195753 1101 52-53 1.0225109636589345 1101 54-55 1.0478249639635706 1101 56-57 1.018779532316831 1101 58-59 1.0833692073680226 1101 60-61 1.014907607457726 1101 62-63 1.0229683527435292 1101 64-65 0.8418945177220394 1101 66-67 1.0317379262990976 1101 68-69 0.9759682288743718 1101 70-71 0.7884032436761785 1101 72-73 0.8313864016584773 1101 74-75 0.9691425054520053 1101 76-77 1.0100885027526267 1102 1 0.9922527216085228 1102 2 1.10476859897598 1102 3 1.0504727840163284 1102 4 0.8273010689818605 1102 5 1.0530855007021813 1102 6 1.0303029885880477 1102 7 1.0729314441593019 1102 8 0.8662456800199294 1102 9 1.0649747893376684 1102 10-11 1.0118183868053023 1102 12-13 1.2150199466710419 1102 14-15 1.0920070129285406 1102 16-17 1.1028329887015147 1102 18-19 1.1479933317795883 1102 20-21 1.0867312150360533 1102 22-23 1.039566779314974 1102 24-25 1.1678845419773083 1102 26-27 0.9401653776116277 1102 28-29 1.0510135871630837 1102 30-31 1.0992719887155076 1102 32-33 1.0420069629304862 1102 34-35 1.0070326031882573 1102 36-37 1.0692482415268358 1102 38-39 1.024384991971253 1102 40-41 1.1197914265956541 1102 42-43 1.133521086336149 1102 44-45 1.0997443522606254 1102 46-47 1.1481561189091316 1102 48-49 1.083771496546337 1102 50-51 1.0467697193883474 1102 52-53 1.019524101352463 1102 54-55 1.1500866459125803 1102 56-57 1.2113495187544814 1102 58-59 1.1478556952278467 1102 60-61 1.0426298466849744 1102 62-63 0.9907012143991025 1102 64-65 0.927706195860047 1102 66-67 1.0442635598640386 1102 68-69 1.0331403642365338 1102 70-71 0.8585015918764753 1102 72-73 0.8056858113756995 1102 74-75 0.9281222683952173 1102 76-77 1.016054726334179 1103 1 0.270701887998527 1103 2 0.4280394376051859 1103 3 0.41484788559279195 1103 4 0.34936891812593274 1103 5 0.2960316372774443 1103 6 0.3694706889616626 1103 7 0.45872152733277716 1103 8 0.2842376446558035 1103 9 0.39812360655929524 1103 10-11 0.4658884603292961 1103 12-13 0.3951594149219986 1103 14-15 0.44881718543837223 1103 16-17 0.41100834892465343 1103 18-19 0.41734992849683294 1103 20-21 0.3912479703491698 1103 22-23 0.32065959624397067 1103 24-25 0.4037173162059773 1103 26-27 0.36058209694757437 1103 28-29 0.398523638313641 1103 30-31 0.4983803383357994 1103 32-33 0.5969964684319251 1103 34-35 0.6069755120048796 1103 36-37 0.6195090875783507 1103 38-39 0.32899435261019505 1103 40-41 0.37315311567975584 1103 42-43 0.4889380117189468 1103 44-45 0.4639005579981763 1103 46-47 0.47088579516553963 1103 48-49 0.3471525031014089 1103 50-51 0.38302683437128593 1103 52-53 0.4368631984412765 1103 54-55 0.27620317675038564 1103 56-57 0.18377049515725474 1103 58-59 0.14394115234857097 1103 60-61 0.10107156297651798 1103 62-63 0.09619997973859995 1103 64-65 0.046659704745295016 1103 66-67 -0.02898412743370926 1103 68-69 0.02806362451815403 1103 70-71 -0.2796664803075224 1103 72-73 -0.39126163926913193 1103 74-75 -0.23871675993414243 1103 76-77 -0.25280400100009004 1104 1 0.49757721200409577 1104 2 0.3232422235364467 1104 3 0.3915113986801515 1104 4 0.4193963479767575 1104 5 0.3779294631046284 1104 6 0.4950496313176487 1104 7 0.3845239156075735 1104 8 0.289332786616292 1104 9 0.34941203687642286 1104 10-11 0.5206866177764624 1104 12-13 0.5429338055219901 1104 14-15 0.6122345872663715 1104 16-17 0.48336614810364864 1104 18-19 0.5257665711298074 1104 20-21 0.40048948510944626 1104 22-23 0.3968630101957302 1104 24-25 0.42835431766418 1104 26-27 0.13259291255662475 1104 28-29 0.4185713283392474 1104 30-31 0.5608906144222416 1104 32-33 0.5438337248718312 1104 34-35 0.5406674639087612 1104 36-37 0.4439580422701255 1104 38-39 0.35521375907242714 1104 40-41 0.42128383378840084 1104 42-43 0.5181517462306893 1104 44-45 0.49817057626377803 1104 46-47 0.4556527043253844 1104 48-49 0.297746427205265 1104 50-51 0.2110289565235206 1104 52-53 0.371448215255878 1104 54-55 0.4385091889460213 1104 56-57 0.2888920517136917 1104 58-59 0.15779915682468015 1104 60-61 0.28807949005067357 1104 62-63 0.18504511383759592 1104 64-65 0.1141860586954877 1104 66-67 0.15864081405436892 1104 68-69 -0.0504200698595767 1104 70-71 0.03928886734539461 1104 72-73 -0.11768301856027108 1104 74-75 -0.11960602437749301 1104 76-77 -0.11602266443950882 1105 1 0.1633049486694702 1105 2 0.36843260265972333 1105 3 0.44271648282150267 1105 4 0.3333325761515553 1105 5 0.09727055209256719 1105 6 0.3629075143306082 1105 7 0.2573622739729018 1105 8 0.27787318608832834 1105 9 0.2611450310784633 1105 10-11 0.36275415798901633 1105 12-13 0.5784748726808644 1105 14-15 0.6497311019057719 1105 16-17 0.5777092808054078 1105 18-19 0.5046775434205841 1105 20-21 0.4121365549540883 1105 22-23 0.3306018310811112 1105 24-25 0.38619581561582805 1105 26-27 0.1403333958098827 1105 28-29 0.30267640483601355 1105 30-31 0.5137724055728015 1105 32-33 0.524833262219218 1105 34-35 0.32003993992218227 1105 36-37 0.441261665675313 1105 38-39 0.5120556489752417 1105 40-41 0.5900022418391728 1105 42-43 0.4357224708808012 1105 44-45 0.26563681742554834 1105 46-47 0.2979177667172088 1105 48-49 0.27233024974108844 1105 50-51 0.28186949072600953 1105 52-53 0.14981540501017676 1105 54-55 0.02626283592572065 1105 56-57 0.025838685816449214 1105 58-59 0.06826703693445779 1105 60-61 0.07154193454450919 1105 62-63 0.10616328380123363 1105 64-65 0.13860125827022074 1105 66-67 0.046994937296719996 1105 68-69 -0.042055977712610826 1105 70-71 -0.16619694662936624 1105 72-73 -0.5514915466576902 1105 74-75 -0.5497569675569416 1105 76-77 -0.6515983090268449 1106 1 0.06254813641372436 1106 2 0.07756966368263818 1106 3 0.07996344321649218 1106 4 0.30502465499773734 1106 5 0.14320917120850396 1106 6 0.07185418233321172 1106 7 -0.06289965118308061 1106 8 0.10654793982169508 1106 9 0.08623557100133894 1106 10-11 -0.13995948590952878 1106 12-13 0.1663895018415502 1106 14-15 0.14547660033883147 1106 16-17 0.05450350137746085 1106 18-19 -0.06954574108070943 1106 20-21 -0.07093954857016627 1106 22-23 -0.12890748524493745 1106 24-25 -0.37172750076977934 1106 26-27 -0.4730715116788957 1106 28-29 -0.1570036349117956 1106 30-31 -0.03879921513039619 1106 32-33 -0.10327689796521611 1106 34-35 -0.1198761741301837 1106 36-37 -0.040910940376960525 1106 38-39 0.09835780574129416 1106 40-41 0.08628588310133978 1106 42-43 0.05539839414024428 1106 44-45 -0.12801933495320483 1106 46-47 -0.06332886418483952 1106 48-49 -0.23457801755419538 1106 50-51 -0.18127348508647145 1106 52-53 -0.27549445637022885 1106 54-55 -0.3475787496916922 1106 56-57 -0.12074557428823951 1106 58-59 -0.27263514343510664 1106 60-61 -0.47681963136418304 1106 62-63 -0.492143413707943 1106 64-65 -0.36581233883424247 1106 66-67 -0.4471738417689153 1106 68-69 -0.5968971006054389 1106 70-71 -0.7209368960621951 1106 72-73 -0.786658115555948 1106 74-75 -0.6526939537701857 1106 76-77 -0.504624786479944 1107 1 0.009048145952633035 1107 2 -0.10219122497841937 1107 3 -0.020980991412550054 1107 4 -0.045615364113253065 1107 5 0.034680062200372674 1107 6 -0.2346182141468489 1107 7 -0.11479772755902928 1107 8 -0.20849765200105708 1107 9 -0.13745591921630762 1107 10-11 0.003342563959876088 1107 12-13 0.10652965325301977 1107 14-15 0.12830055609821756 1107 16-17 -0.05701273674349494 1107 18-19 0.10165273534865094 1107 20-21 -0.030763750682154978 1107 22-23 -0.02164352547091397 1107 24-25 -0.07615112689774861 1107 26-27 -0.1835768124074164 1107 28-29 -0.16929316724827004 1107 30-31 0.059390134639720316 1107 32-33 0.15558194613505805 1107 34-35 -0.010848657289869834 1107 36-37 0.1323289655943114 1107 38-39 0.14528445321402472 1107 40-41 0.05314813881203406 1107 42-43 0.10528054717913093 1107 44-45 0.012956984505581914 1107 46-47 -0.02426683724199208 1107 48-49 -0.016558132668052394 1107 50-51 0.05079171292047846 1107 52-53 -0.07442489618015102 1107 54-55 -0.13055609520496603 1107 56-57 0.028318285783477393 1107 58-59 -0.046465300312782176 1107 60-61 -0.07444110518320812 1107 62-63 -0.01582271071546515 1107 64-65 0.017169859078975946 1107 66-67 0.054026090380428826 1107 68-69 -0.18117240089434006 1107 70-71 -0.1790937596367641 1107 72-73 -0.2528571062567586 1107 74-75 -0.40708724203011215 1107 76-77 -0.1357422319775381 1108 1 0.05073455087238443 1108 2 0.23840558909076748 1108 3 0.10516667507420863 1108 4 -0.016750967679570294 1108 5 -0.021921391634897702 1108 6 0.17843075478965886 1108 7 0.25256455049855475 1108 8 0.1668783568102512 1108 9 0.19676121649410305 1108 10-11 0.13432492405001994 1108 12-13 0.2635152468814681 1108 14-15 0.09759942192444271 1108 16-17 0.20798362199071363 1108 18-19 0.09300129207973384 1108 20-21 0.28252494473912293 1108 22-23 0.22537118649985644 1108 24-25 0.42743190613916937 1108 26-27 0.3396219197878949 1108 28-29 0.43857834988296673 1108 30-31 0.5009374094252692 1108 32-33 0.5612194465376774 1108 34-35 0.5251630489672152 1108 36-37 0.5789774495000728 1108 38-39 0.6017045508015144 1108 40-41 0.48476501008877193 1108 42-43 0.5505029668493542 1108 44-45 0.5474908749879326 1108 46-47 0.5352010823029225 1108 48-49 0.4820993715211692 1108 50-51 0.4012136598153475 1108 52-53 0.5002542442768636 1108 54-55 0.6546590105095831 1108 56-57 0.709552568831203 1108 58-59 0.6986854724447369 1108 60-61 0.724091920351217 1108 62-63 0.746234906914701 1108 64-65 0.6400304681248699 1108 66-67 0.7054350874100699 1108 68-69 0.5457989497678035 1108 70-71 0.6109390510354835 1108 72-73 0.6794616023076436 1108 74-75 0.9143746613738202 1108 76-77 0.8257210383740734 1109 1 -0.2949970460403435 1109 2 -0.3385455601555094 1109 3 -0.5235052627999863 1109 4 -0.3070452272798505 1109 5 -0.28968056952679433 1109 6 -0.4768970519459259 1109 7 -0.48810084969655776 1109 8 -0.3679400046596655 1109 9 -0.4513415311048732 1109 10-11 -0.3128904201668874 1109 12-13 -0.2656075828111 1109 14-15 -0.30439594029787287 1109 16-17 -0.1998665997970548 1109 18-19 -0.14282130021248918 1109 20-21 -0.14675661113845706 1109 22-23 -0.04324876898539287 1109 24-25 0.0277063341844368 1109 26-27 -0.18088682684192037 1109 28-29 -0.0051622512815470145 1109 30-31 0.05681876863685176 1109 32-33 0.02779806568003096 1109 34-35 0.0133818166938795 1109 36-37 0.07955091309793971 1109 38-39 -0.0020222057291263695 1109 40-41 0.031424570076261205 1109 42-43 -0.01771969771287374 1109 44-45 -0.08094614657992238 1109 46-47 -0.2962847473242469 1109 48-49 -0.0823572576500986 1109 50-51 -0.08791546924384619 1109 52-53 -0.020694060745217513 1109 54-55 0.10887381270151408 1109 56-57 0.12424000982315775 1109 58-59 0.10603442300634924 1109 60-61 0.20437049751719982 1109 62-63 0.17772085298118157 1109 64-65 0.24279398047005785 1109 66-67 0.23447116981727234 1109 68-69 0.06538763178932072 1109 70-71 0.14893407092622724 1109 72-73 -0.18114172021042307 1109 74-75 -0.06437089525362438 1109 76-77 0.03702966289786147 1110 1 -0.23891744344518173 1110 2 -0.19226717630756696 1110 3 -0.44704469752741005 1110 4 -0.4996764351819962 1110 5 -0.4900041197728058 1110 6 -0.3917328386527785 1110 7 -0.24827443840146657 1110 8 -0.48254244778498645 1110 9 -0.5346503701191949 1110 10-11 -0.42123892871624946 1110 12-13 -0.3220819099493397 1110 14-15 -0.3389357455370643 1110 16-17 -0.395073652809522 1110 18-19 -0.4235352054403094 1110 20-21 -0.5688556672906984 1110 22-23 -0.5300136719484811 1110 24-25 -0.7235296435217933 1110 26-27 -0.8395773549169121 1110 28-29 -0.6833499757424164 1110 30-31 -0.6080127949115521 1110 32-33 -0.626823308456899 1110 34-35 -0.6331167344812201 1110 36-37 -0.5386099633369525 1110 38-39 -0.6414511896597261 1110 40-41 -0.6974137460209491 1110 42-43 -0.6488560877119802 1110 44-45 -0.5523379081550388 1110 46-47 -0.6610761064042059 1110 48-49 -0.7514550558642092 1110 50-51 -0.6453642198083429 1110 52-53 -0.6885068926150844 1110 54-55 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26-27 -0.4373810072703037 2314 28-29 -0.6647629070476597 2314 30-31 -0.6397914102478452 2314 32-33 -0.8675914692681346 2314 34-35 -0.9149738709284776 2314 36-37 -1.4801902567815581 2314 38-39 -0.9853287864650397 2314 40-41 -0.720184868199798 2314 42-43 -0.5946316519422474 2314 44-45 -0.5822207995266915 2314 46-47 -0.47908660579846796 2314 48-49 -0.3035594216379778 2314 50-51 -0.23221978583337943 2314 52-53 -0.3031364973173787 2314 54-55 -0.49518824929397454 2314 56-57 -0.5387163083745534 2314 58-59 -0.45127986812035203 2314 60-61 -0.3385387038648666 2314 62-63 -0.35353283116889145 2314 64-65 -0.3302773890110231 2314 66-67 -0.1311108830692369 2314 68-69 -0.2055317909994585 2314 70-71 -0.144266587661253 2314 72-73 -0.13172095676887707 2314 74-75 -0.19948864194935112 2314 76-77 -0.16830911768751022 2315 1 -1.1116559470499467 2315 2 -1.0089816868681254 2315 3 -0.8466925377685897 2315 4 -0.7369309457816868 2315 5 -0.8857272604977808 2315 6 -1.0090328906385793 2315 7 -1.4088045630956358 2315 8 -1.3297766010850438 2315 9 -0.8088359966002585 2315 10-11 -1.106395450324925 2315 12-13 -1.0701862546445327 2315 14-15 -1.02746873499445 2315 16-17 -0.9772898132832282 2315 18-19 -1.0148452932291292 2315 20-21 -0.9499640801844862 2315 22-23 -1.0961460328799433 2315 24-25 -1.2622174815191798 2315 26-27 -1.0494370780550284 2315 28-29 -0.9453241576509654 2315 30-31 -1.1121525799629879 2315 32-33 -1.0874960842702563 2315 34-35 -1.2114545065016458 2315 36-37 -1.3151874583780767 2315 38-39 -1.1117413382530117 2315 40-41 -1.122822604218431 2315 42-43 -1.3522546022357176 2315 44-45 -1.152349257626799 2315 46-47 -1.0450380308832514 2315 48-49 -0.9570641083072466 2315 50-51 -0.8891893570619374 2315 52-53 -0.9315346316372448 2315 54-55 -1.165726138042995 2315 56-57 -1.2491244434122564 2315 58-59 -1.3200853729505866 2315 60-61 -1.3205018408080527 2315 62-63 -1.190532248143846 2315 64-65 -1.0664159540457447 2315 66-67 -1.156725320846924 2315 68-69 -1.2780085444078786 2315 70-71 -1.277085411608116 2315 72-73 -1.1195820427361838 2315 74-75 -1.205795554735456 2315 76-77 -1.1208645065255318 2316 1 -2.0691778658597215 2316 2 -1.8353721806451446 2316 3 -1.852991801601732 2316 4 -2.7964902008514656 2316 5 -2.418982341177397 2316 6 -2.156599616678534 2316 7 -1.915435578007127 2316 8 -1.5741260147831255 2316 9 -1.7821407726298162 2316 10-11 -1.7839560798378216 2316 12-13 -2.5901248149858915 2316 14-15 -3.7605725884675287 2316 16-17 -2.903639070561475 2316 18-19 -2.339605879534247 2316 20-21 -1.9887387295300059 2316 22-23 -2.3272552129611626 2316 24-25 -2.2580853375321546 2316 26-27 -2.1672680895047662 2316 28-29 -2.619594842170436 2316 30-31 -2.6385735082954653 2316 32-33 -2.6985616174487355 2316 34-35 -2.7901094818543015 2316 36-37 -2.722305410150124 2316 38-39 -2.707572525354532 2316 40-41 -2.4130700200057476 2316 42-43 -2.5976271554913843 2316 44-45 -2.441678828764008 2316 46-47 -2.5498239809205785 2316 48-49 -2.2529149921273763 2316 50-51 -2.484175059712328 2316 52-53 -2.487949543575766 2316 54-55 -2.5657904528776534 2316 56-57 -2.3643663540548623 2316 58-59 -2.5921488229887153 2316 60-61 -2.280915923849676 2316 62-63 -2.2745567028460414 2316 64-65 -2.3320347787879 2316 66-67 -2.2752873065325865 2316 68-69 -2.2000460793979997 2316 70-71 -2.025041376220056 2316 72-73 -1.9239646988539114 2316 74-75 -2.060337691770126 2316 76-77 -2.575815915409855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57338.0 3 5882.0 4 7990.0 5 9949.0 6 11047.0 7 11251.0 8 13408.0 9 15019.0 10 19046.0 11 21465.0 12 15761.0 13 12306.0 14 12149.0 15 12304.0 16 12313.0 17 12913.0 18 13504.0 19 14146.0 20 15175.0 21 16346.0 22 18029.0 23 20469.0 24 23695.0 25 27479.0 26 32276.0 27 37660.0 28 44204.0 29 51746.0 30 61751.0 31 73649.0 32 89392.0 33 112351.0 34 152284.0 35 203092.0 36 277518.0 37 349469.0 38 281038.0 39 67458.0 40 424.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.86270047782872 25.699626594161725 29.162910890831967 22.27476203717759 2 22.50964464277318 26.137776772699894 28.926091697434735 22.426486887092185 3 22.701435235710672 26.017448174282908 28.564097029439594 22.717019560566822 4 22.435288806562394 26.18911128587577 29.03010689506298 22.345493012498856 5 22.742126681573684 26.08084451396872 28.66871806079214 22.50831074366545 6 22.614699359775866 26.013986765596282 28.932479104044482 22.438834770583366 7 22.570106119268292 25.8075163523097 28.750730563648048 22.871646964773955 8 22.493452261522588 26.222438589199726 28.298322489108003 22.985786660169687 9 22.354269990711472 26.06744169590418 28.9126242463708 22.665664067013548 10-11 23.01227261489733 26.04511155619731 28.34592453519744 22.59669129370792 12-13 23.167273302170766 25.6557328958571 28.241536326247633 22.9354574757245 14-15 22.79893108354856 25.85888914637819 28.441193362149747 22.900986407923504 16-17 22.86860965763684 25.822211176564096 28.680275216006052 22.628903949793006 18-19 22.961986214259355 25.943828838995593 28.07633298582376 23.017851960921295 20-21 22.765231409954996 26.178477866595017 28.06354537515954 22.992745348290455 22-23 23.129412402207542 26.321825774194725 27.4985708569462 23.050190966651538 24-25 23.14128926863514 26.558692032681268 27.06344452145366 23.23657417722993 26-27 23.066810601078434 26.65959534008983 27.07892634960343 23.194667709228305 28-29 23.414253293162187 26.487095138980937 27.056054510476855 23.042597057380014 30-31 22.944615357058094 26.488372717868803 27.14642359758364 23.420588327489465 32-33 22.97083254406152 26.8154483220641 27.0242638948784 23.189455238995986 34-35 23.091413583445252 26.31076561098455 27.038141500304295 23.559679305265902 36-37 22.971526348656745 26.413791451164983 27.47732992038519 23.137352279793085 38-39 23.02456739687524 26.423723105337594 27.498658681755117 23.053050816032044 40-41 22.847610496216962 26.582124008338575 27.26788461237038 23.30238088307408 42-43 22.8145102493045 26.85755768648575 27.08011260530997 23.24781945889978 44-45 22.89854546724816 26.452181530416745 27.402164556837306 23.24710844549779 46-47 22.78828179651413 26.58905316937331 27.214069403852026 23.408595630260532 48-49 22.859703184946707 26.657756273810744 27.02274053470418 23.459800006538366 50-51 22.732252405259796 26.931989854486314 26.960273855416126 23.37548388483776 52-53 22.879867203918668 26.646894467247996 27.11155533737407 23.361682991459272 54-55 22.824734058395855 26.683333878257397 27.125035886608618 23.366896176738134 56-57 22.868788054057333 26.927544156757584 26.969081592735627 23.234586196449456 58-59 22.803955254160773 26.84250998039302 26.947576177471404 23.4059585879748 60-61 22.696480059762937 26.666859248072615 26.944422797697264 23.69223789446718 62-63 22.992236087090014 26.558341990633323 26.848685325826132 23.600736596450535 64-65 22.83496851858777 26.45197755041762 26.81922523726104 23.893828693733568 66-67 22.68849397401504 26.617633227787156 26.984611457209013 23.709261340988792 68-69 22.898817714044103 26.270478145894693 27.09853965558016 23.732164484481043 70-71 22.706159626689022 26.072607112863576 27.205949391729895 24.015283868717507 72-73 22.977376222517854 25.955039213322895 27.005440084023107 24.062144480136144 74-75 22.80172259267226 26.125757331325733 26.86027909227791 24.212240983724097 76-77 22.751968596557855 25.757163294090233 27.32516207523517 24.165706034116738 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14373.0 1 8494.5 2 2462.0 3 2381.5 4 2455.0 5 2503.0 6 2568.5 7 2679.0 8 2864.5 9 2957.0 10 3035.5 11 3164.0 12 3384.0 13 3554.0 14 3713.5 15 4075.0 16 4646.0 17 5015.0 18 5299.5 19 6122.0 20 7209.5 21 8603.5 22 9448.0 23 10667.0 24 13073.5 25 15908.5 26 17556.0 27 18483.5 28 22397.5 29 26474.5 30 27565.0 31 30884.5 32 37368.0 33 42922.0 34 49088.0 35 52864.0 36 56460.0 37 63222.0 38 67594.0 39 68800.0 40 72244.5 41 79602.5 42 88980.5 43 94445.0 44 93567.5 45 94012.5 46 98395.0 47 102881.5 48 104308.0 49 104895.5 50 106882.5 51 106746.5 52 105211.0 53 105057.5 54 101288.5 55 91917.5 56 82010.5 57 77859.0 58 75461.5 59 66231.5 60 54905.0 61 50411.0 62 44742.5 63 33673.0 64 24791.5 65 21311.0 66 18426.5 67 14185.0 68 11041.0 69 7856.5 70 6459.0 71 5327.5 72 3629.0 73 2813.5 74 2565.0 75 2245.0 76 1517.0 77 916.0 78 723.0 79 572.0 80 386.0 81 284.5 82 173.5 83 129.0 84 116.0 85 93.5 86 74.5 87 65.0 88 57.5 89 43.5 90 38.0 91 39.0 92 34.0 93 33.0 94 30.5 95 27.0 96 30.0 97 27.5 98 24.5 99 56.5 100 89.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0030896038859157047 2 0.008552381771157965 3 0.012671853619045572 4 0.020239144296143458 5 0.01535846569375488 6 0.014686812675077553 7 0.0192988300699952 8 0.02077646671108532 9 0.019433160673730663 10-11 0.02328397131414734 12-13 0.021895888408880865 14-15 0.008664323940937519 16-17 0.006313538375566875 18-19 0.011821093128720957 20-21 0.011015109506308166 22-23 0.01408232495826796 24-25 0.023216806012279607 26-27 0.014977862316504394 28-29 0.008238943695775212 30-31 0.008328497431598856 32-33 0.010074795280159907 34-35 0.012044977468280066 36-37 0.010365844921586749 38-39 0.018716730787141516 40-41 0.013477837241458367 42-43 0.01629877991990314 44-45 0.01853762331549423 46-47 0.017642085957257792 48-49 0.014462928335518443 50-51 0.026194467728415757 52-53 0.031343807538275266 54-55 0.02547803784182661 56-57 0.01658982956132998 58-59 0.018716730787141516 60-61 0.021224235390203537 62-63 0.026619847973578067 64-65 0.021067516352512163 66-67 0.020866020446908964 68-69 0.030090055236744257 70-71 0.05684423381405779 72-73 0.06846383103717554 74-75 0.07952371741139554 76-77 0.07582962580867024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2233296.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.7202607528984 #Duplication Level Percentage of deduplicated Percentage of total 1 73.98148231011228 36.04397109034611 2 12.687247495078887 12.36252012393601 3 5.123920707900036 7.489162588981965 4 2.5564067519472924 4.981952141813686 5 1.4751679629012309 3.593528390343496 6 0.9239401248030046 2.700876228028072 7 0.6389747573846953 2.179171175601164 8 0.43371352628165505 1.690450887400103 9 0.31967037980939794 1.401698183336274 >10 1.6578765526848875 14.518229098930806 >50 0.12368087665342918 4.150216455962872 >100 0.07312072100648082 6.182008326095739 >500 0.003506109049959387 1.1563516596536352 >1k 0.0011071923315660852 0.6982882398172567 >5k 9.226602763050711E-5 0.2545645874909956 >10k+ 9.226602763050711E-5 0.5970108222616832 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13281 0.5946815827369054 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5663 0.2535714029846469 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.955373582364362E-5 0.0 2 0.0 0.0 0.0 7.612067545009708E-4 0.0 3 0.0 0.0 0.0 0.0013433060373546544 0.0 4 1.3433060373546543E-4 0.0 0.0 0.002820942678444774 0.0 5 2.6866120747093085E-4 0.0 0.0 0.005552331621065904 0.0 6 3.1343807538275264E-4 0.0 0.0 0.00940314226148258 0.0 7 3.1343807538275264E-4 0.0 0.0 0.01907494573043609 0.0 8 3.1343807538275264E-4 0.0 0.0 0.027045228218740374 0.0 9 3.582149432945745E-4 0.0 0.0 0.037299130970547566 0.0 10 3.582149432945745E-4 0.0 0.0 0.04849334794850302 0.0 11 4.477686791182181E-4 0.0 0.0 0.06201596205787321 0.0 12 5.373224149418617E-4 0.0 0.0 0.08167300707116298 0.0 13 5.373224149418617E-4 0.0 0.0 0.10544952393234036 0.0 14 5.373224149418617E-4 0.0 0.0 0.13545002543326098 0.0 15 5.820992828536835E-4 0.0 0.0 0.1687640151596564 0.0 16 5.820992828536835E-4 0.0 0.0 0.20373474899878924 0.0 17 6.268761507655053E-4 0.0 0.0 0.23561587895200636 0.0 18 6.716530186773272E-4 0.0 0.0 0.2713478195456402 0.0 19 8.955373582364362E-4 0.0 0.0 0.30828873557289316 0.0 20 9.850910940600798E-4 0.0 0.0 0.3484535860897973 0.0 21 9.850910940600798E-4 0.0 0.0 0.3916632636247054 0.0 22 0.0011194216977955453 0.0 0.0 0.4438730916098896 0.0 23 0.0012089754336191889 0.0 0.0 0.4966202420100157 0.0 24 0.0012089754336191889 0.0 0.0 0.5469046646749915 0.0 25 0.0012089754336191889 0.0 0.0 0.6006816830370896 0.0 26 0.0012089754336191889 0.0 0.0 0.6588020575866342 0.0 27 0.0012089754336191889 0.0 0.0 0.7162955559854135 0.0 28 0.0012089754336191889 0.0 0.0 0.7722666408751907 0.0 29 0.0012089754336191889 0.0 0.0 0.8306556766322064 0.0 30 0.0012985291694428325 0.0 0.0 0.8961194575192899 0.0 31 0.001388082905266476 4.477686791182181E-5 0.0 0.9645832885564655 0.0 32 0.001432859773178298 4.477686791182181E-5 0.0 1.0368531533661458 0.0 33 0.001432859773178298 4.477686791182181E-5 0.0 1.1157947714946876 0.0 34 0.001432859773178298 4.477686791182181E-5 0.0 1.1995722913577063 0.0 35 0.001432859773178298 4.477686791182181E-5 0.0 1.285588654616316 0.0 36 0.001432859773178298 4.477686791182181E-5 0.0 1.3786797630049936 0.0 37 0.0014776366410901196 4.477686791182181E-5 0.0 1.472039532601142 0.0 38 0.0015224135090019415 4.477686791182181E-5 0.0 1.5642351036315831 0.0 39 0.0015671903769137634 4.477686791182181E-5 0.0 1.6603710390382644 0.0 40 0.0015671903769137634 4.477686791182181E-5 0.0 1.7617010911227173 0.0 41 0.0015671903769137634 4.477686791182181E-5 0.0 1.8663894083005568 0.0 42 0.0017015209806492287 4.477686791182181E-5 0.0 1.9834809178899706 0.0 43 0.0017015209806492287 4.477686791182181E-5 0.0 2.11176664445734 0.0 44 0.0017910747164728725 4.477686791182181E-5 0.0 2.2469032318152182 0.0 45 0.0017910747164728725 4.477686791182181E-5 0.0 2.387233935850868 0.0 46 0.0018358515843846942 4.477686791182181E-5 0.0 2.535221484299439 0.0 47 0.0019254053202083378 4.477686791182181E-5 4.477686791182181E-5 2.681776172974832 0.0 48 0.0019254053202083378 4.477686791182181E-5 4.477686791182181E-5 2.8332115402526132 0.0 49 0.0020149590560319813 4.477686791182181E-5 4.477686791182181E-5 2.9859454366998373 0.0 50 0.0020597359239438034 4.477686791182181E-5 4.477686791182181E-5 3.1377837957888253 0.0 51 0.0020597359239438034 4.477686791182181E-5 4.477686791182181E-5 3.3039955294775076 0.0 52 0.0020597359239438034 4.477686791182181E-5 4.477686791182181E-5 3.4764312478059334 0.0 53 0.0020597359239438034 4.477686791182181E-5 4.477686791182181E-5 3.666553828959529 0.0 54 0.0020597359239438034 4.477686791182181E-5 4.477686791182181E-5 3.8629003947528675 0.0 55 0.002104512791855625 4.477686791182181E-5 4.477686791182181E-5 4.065650052657596 0.0 56 0.0022836202635029123 4.477686791182181E-5 4.477686791182181E-5 4.284564159878493 0.0 57 0.002373173999326556 4.477686791182181E-5 4.477686791182181E-5 4.503657374571038 0.0 58 0.002373173999326556 4.477686791182181E-5 4.477686791182181E-5 4.73425824431692 0.0 59 0.002373173999326556 4.477686791182181E-5 4.477686791182181E-5 4.962082948252269 0.0 60 0.0024627277351501995 4.477686791182181E-5 4.477686791182181E-5 5.195549537544508 0.0 61 0.002507504603062021 4.477686791182181E-5 4.477686791182181E-5 5.439135698984819 0.0 62 0.002507504603062021 4.477686791182181E-5 4.477686791182181E-5 5.691050357856728 0.0 63 0.002507504603062021 4.477686791182181E-5 8.955373582364362E-5 5.9525920433296795 0.0 64 0.002507504603062021 4.477686791182181E-5 8.955373582364362E-5 6.229313087024738 0.0 65 0.002507504603062021 4.477686791182181E-5 8.955373582364362E-5 6.513601421396895 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCGCG 350 0.0 27.385118 56 AATTCGC 360 0.0 26.624422 55 CGCGGAA 380 0.0 26.159084 59 CTCGCGC 435 0.0 25.2966 36 TCGCGCT 410 0.0 25.107523 37 CGATCGT 410 0.0 24.240118 1 TCGCGGA 420 0.0 22.822977 58 CAATTCG 425 0.0 22.55245 54 CCTCGCG 510 0.0 22.27153 35 ACTCGAG 465 0.0 22.137814 14 CGCTCCG 485 0.0 21.22539 25 AACTCGA 505 0.0 21.087704 13 ATCGTTC 510 0.0 20.879091 3 CGCGCTT 495 0.0 20.797062 38 CGGTTAC 350 0.0 20.283003 4 GACGTAT 335 0.0 20.13434 39 AACGCGA 90 0.004861467 19.723118 53 TCGCGAT 90 0.0048652473 19.720469 26 CGTAAGT 475 0.0 19.431591 39 GATCGTT 535 0.0 18.57654 2 >>END_MODULE