FastQCFastQC Report
Thu 26 May 2016
SRR936166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233296
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111030.4971575644249575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83990.3760809135913914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59440.2661537028678688No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14250.052.418087100-101
GGTATCA89100.041.752631
CGGTATC10900.039.315002100-101
CGTACAT15800.038.611717100-101
TACGCAG10100.037.71472100-101
GCGTATC3950.036.91657100-101
TTTACGC2700.036.37122398-99
CCGTACA7400.034.5851100-101
ACCGTAT2650.033.6886198-99
AGTATCA30850.033.37669100-101
AGGTATC22300.033.228962100-101
CCGGTAT3450.032.77833100-101
TCGTATC6550.032.712482100-101
GGGTATC11750.032.671883100-101
ACGTATC4950.032.46466100-101
GTATCAA176850.031.2674261
CCCGGTA3150.031.17533598-99
CCGTATC7450.031.15736100-101
TACCGTA2300.031.05210998-99
TCGGTAT3850.030.918726100-101
Produced by FastQC (version 0.11.5)