FastQCFastQC Report
Thu 26 May 2016
SRR936165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078684
Sequences flagged as poor quality0
Sequence length77
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69580.22600565696252037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC4400.021.7778264
CTCGCGC5750.020.98765436
CGCGGAA5300.020.76357759
ATTCGCG5500.020.65531556
TCGCGCT6150.020.20170837
CGATCGT5950.019.6846921
CGGTATA1501.32780515E-518.92942610
CAATTCG5850.018.81266854
AATTCGC5900.018.65232855
CGCGCTT6700.018.01237738
CCTCGCG6900.017.48999635
TCGCGGA6350.016.77064358
CGCTCCG7050.016.6149425
GACGTAT7950.016.07371939
AACTCGA7950.015.62773613
GCGTAAG9600.015.52905538
ACTCGAG8100.015.33908214
ACGTATT8350.015.30272740
GGGCGGT7350.014.9694551
CGTATTA8800.014.92354141
Produced by FastQC (version 0.11.5)