##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936165_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3078684 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.398983136950726 39.0 36.0 41.0 30.0 41.0 2 36.25678699080516 39.0 36.0 41.0 29.0 41.0 3 36.16693236460773 39.0 36.0 41.0 29.0 41.0 4 36.0410042082916 39.0 35.0 41.0 28.0 41.0 5 35.961700518793094 39.0 35.0 41.0 28.0 41.0 6 35.864321573763334 39.0 35.0 40.0 28.0 41.0 7 35.68768538765265 39.0 35.0 40.0 27.0 41.0 8 35.51111578843428 38.0 35.0 40.0 27.0 41.0 9 35.410360075928544 38.0 35.0 40.0 27.0 41.0 10-11 35.61599534086642 39.0 35.0 40.0 27.0 41.0 12-13 35.784488599674404 39.0 35.0 41.0 28.0 41.0 14-15 35.714953044872416 39.0 35.0 41.0 27.0 41.0 16-17 35.540795028005476 39.0 35.0 41.0 27.0 41.0 18-19 35.37848590501656 39.0 35.0 41.0 26.0 41.0 20-21 35.165018235064075 39.0 35.0 41.0 25.5 41.0 22-23 34.89615676698226 39.0 34.0 41.0 24.0 41.0 24-25 34.652874897196334 39.0 34.0 40.5 24.0 41.0 26-27 33.705352027034934 37.5 33.0 39.5 22.5 40.5 28-29 33.72951900877128 38.0 33.0 39.5 22.0 40.5 30-31 34.084964224974044 38.0 33.0 40.0 22.0 41.0 32-33 33.91838688218732 38.0 33.0 40.0 21.5 41.0 34-35 33.63778744424566 38.0 33.0 40.0 20.0 41.0 36-37 33.33919395429996 37.0 33.0 40.0 18.5 41.0 38-39 33.053842973166454 37.0 32.0 40.0 18.0 41.0 40-41 32.66767342799716 36.0 32.0 39.5 14.5 41.0 42-43 32.30155352091998 36.0 31.5 39.0 12.5 41.0 44-45 31.93054288780531 35.0 31.0 39.0 8.0 40.5 46-47 31.533039116713503 35.0 31.0 38.0 7.5 40.0 48-49 31.072851582039597 35.0 31.0 37.0 4.0 39.5 50-51 30.633816266950426 35.0 30.0 37.0 2.0 39.0 52-53 30.17865311931981 35.0 30.0 36.0 2.0 39.0 54-55 29.713746198050856 34.0 29.5 36.0 2.0 38.5 56-57 29.266806694029007 34.0 29.0 35.5 2.0 37.0 58-59 28.838752856740086 34.0 29.0 35.0 2.0 37.0 60-61 28.489684553530015 34.0 29.0 35.0 2.0 36.0 62-63 28.137353492596187 34.0 28.0 35.0 2.0 36.0 64-65 27.754843790398755 34.0 27.0 35.0 2.0 35.5 66-67 27.37660052152153 33.5 26.5 35.0 2.0 35.0 68-69 27.007772964032682 33.0 25.5 35.0 2.0 35.0 70-71 26.573911450476892 33.0 25.0 35.0 2.0 35.0 72-73 26.076263429439333 33.0 24.5 35.0 2.0 35.0 74-75 25.467014964835627 33.0 21.5 35.0 2.0 35.0 76-77 23.747696093525676 31.0 9.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.0979191503138779 1101 2 1.0654543947300255 1101 3 1.0226491965571824 1101 4 1.1155484519139947 1101 5 1.1529342978268673 1101 6 1.1235021183299523 1101 7 1.1374465826615108 1101 8 1.2028786517263228 1101 9 1.2364207840446895 1101 10-11 1.1777704763911387 1101 12-13 1.2477223706622667 1101 14-15 1.2944716468327684 1101 16-17 1.3825637275722684 1101 18-19 1.324364876759006 1101 20-21 1.3341818173504763 1101 22-23 1.3143250601153156 1101 24-25 1.2815870064961814 1101 26-27 1.2537636044617528 1101 28-29 1.1967393879044224 1101 30-31 1.3003196291305912 1101 32-33 1.2889939404200774 1101 34-35 1.237263055321698 1101 36-37 1.2530365902548155 1101 38-39 1.2722070435458264 1101 40-41 1.2229613891104805 1101 42-43 1.2954396580596992 1101 44-45 1.0654015356744182 1101 46-47 1.0586096485905578 1101 48-49 1.1931745382214949 1101 50-51 1.142956783450174 1101 52-53 1.2074266055797125 1101 54-55 1.0966001158755567 1101 56-57 1.0947276142815454 1101 58-59 1.129789780824943 1101 60-61 1.0661528424745832 1101 62-63 0.9684377993356534 1101 64-65 0.7980353695997486 1101 66-67 0.9708121204375892 1101 68-69 1.0653376356624165 1101 70-71 0.8803354475739447 1101 72-73 0.7918734731168122 1101 74-75 0.8326062230953362 1101 76-77 0.8261887898644389 1102 1 1.1012901058526836 1102 2 1.1969657521602457 1102 3 1.177985110164684 1102 4 1.1631242020794303 1102 5 1.212979767563482 1102 6 1.1316994166747065 1102 7 1.111450802098929 1102 8 1.197102305622785 1102 9 1.1005499341029719 1102 10-11 1.0644609079608927 1102 12-13 1.2218131056106145 1102 14-15 1.1441986505653503 1102 16-17 1.185944353581462 1102 18-19 1.2454343863216621 1102 20-21 1.1640261857650103 1102 22-23 1.2224211334597257 1102 24-25 1.1735943441971557 1102 26-27 1.102841278717321 1102 28-29 1.125215329182069 1102 30-31 1.0845945920975097 1102 32-33 1.0286439434953607 1102 34-35 1.165117455830341 1102 36-37 1.151608176560245 1102 38-39 1.2555341892601746 1102 40-41 1.4057429128060974 1102 42-43 1.3861314412722052 1102 44-45 1.3248439533957637 1102 46-47 1.1134058313854176 1102 48-49 1.1812870847119932 1102 50-51 1.275389799695823 1102 52-53 1.1610092740218896 1102 54-55 1.1974671976684697 1102 56-57 1.2251433555842368 1102 58-59 1.2719230940021973 1102 60-61 1.146309741225128 1102 62-63 1.0065637214576135 1102 64-65 1.1019815884458275 1102 66-67 1.0991387066726226 1102 68-69 1.1034494781723225 1102 70-71 1.142149724152361 1102 72-73 1.080593888331979 1102 74-75 1.1553802179036659 1102 76-77 1.3917627304292104 1103 1 0.7853513052950305 1103 2 0.6615955616777711 1103 3 0.6847550668859625 1103 4 0.7017382024249628 1103 5 0.8007388592578693 1103 6 0.7916687900006991 1103 7 0.8703843003544662 1103 8 0.7690369277628832 1103 9 0.6573574608879511 1103 10-11 0.8229999478645666 1103 12-13 0.8301138375499946 1103 14-15 0.8401889067520258 1103 16-17 0.8342945063323981 1103 18-19 0.8307095073635509 1103 20-21 0.81699565342592 1103 22-23 0.7725078742321116 1103 24-25 0.5925199192910924 1103 26-27 0.55549577460161 1103 28-29 0.5740549672322288 1103 30-31 0.899645041851663 1103 32-33 0.8312604565248094 1103 34-35 0.8424532557121083 1103 36-37 0.8963020804000621 1103 38-39 0.8096781205445325 1103 40-41 0.9240538419006015 1103 42-43 0.9260236372251214 1103 44-45 0.9187850172629268 1103 46-47 0.6730154208148598 1103 48-49 0.7466249402936214 1103 50-51 0.833979491499889 1103 52-53 0.8467522331102835 1103 54-55 0.9381868941552902 1103 56-57 0.8626119090948983 1103 58-59 0.6042456353969534 1103 60-61 0.6676236651193115 1103 62-63 0.6435189570514765 1103 64-65 0.6715550452959782 1103 66-67 0.6944045872635591 1103 68-69 0.5369210843948302 1103 70-71 0.6628397870920821 1103 72-73 0.5860253697992981 1103 74-75 0.6145560245552097 1103 76-77 0.8145744305325664 1104 1 0.4452494367410438 1104 2 0.40985628199351964 1104 3 0.3504996059593708 1104 4 0.205481774405456 1104 5 0.4161490439386881 1104 6 0.24406324108107924 1104 7 0.43011937377000464 1104 8 0.5769093556141556 1104 9 0.44467623700762005 1104 10-11 0.410112568546559 1104 12-13 0.6043035567095103 1104 14-15 0.4916095021988056 1104 16-17 0.5433718334997835 1104 18-19 0.5442565625751854 1104 20-21 0.3864827249804037 1104 22-23 0.4407176997001585 1104 24-25 0.47995915786134447 1104 26-27 0.33745993385627315 1104 28-29 0.26840670970209857 1104 30-31 0.4562981189551536 1104 32-33 0.4583973895450768 1104 34-35 0.5088115176356212 1104 36-37 0.28022088091809394 1104 38-39 0.3864964111462825 1104 40-41 0.41800604619497506 1104 42-43 0.3622871481786447 1104 44-45 0.3131316100287478 1104 46-47 0.21371702079564514 1104 48-49 0.27767932845680576 1104 50-51 0.09136922719581264 1104 52-53 0.22322176568085084 1104 54-55 0.12512031469505658 1104 56-57 0.20281414311059365 1104 58-59 0.19979982778774286 1104 60-61 0.07187770300703278 1104 62-63 0.1270710890827189 1104 64-65 0.051218589202822074 1104 66-67 0.023762811072085555 1104 68-69 -0.010465094301331135 1104 70-71 -0.19356764554387595 1104 72-73 -0.06863290790787246 1104 74-75 -0.17322736001429107 1104 76-77 -0.07717950728839895 1105 1 0.12932884026054126 1105 2 0.3079468360221682 1105 3 0.2695989900984088 1105 4 0.36175652230683397 1105 5 0.3466094090611591 1105 6 -0.004880315769874244 1105 7 0.19348380279826216 1105 8 0.46020023550718037 1105 9 0.21208757308237125 1105 10-11 0.26023016287949474 1105 12-13 0.47895425987248785 1105 14-15 0.2338243525140271 1105 16-17 0.31486558930462394 1105 18-19 0.5029089083181546 1105 20-21 0.5204091043463066 1105 22-23 0.4712263502347511 1105 24-25 0.4162739664373305 1105 26-27 0.22890233595313703 1105 28-29 0.35466849784148025 1105 30-31 0.35633133515187865 1105 32-33 0.46549430342185616 1105 34-35 0.5489952436879335 1105 36-37 0.4997531291574333 1105 38-39 0.47922736729213966 1105 40-41 0.6549827164926185 1105 42-43 0.554717122493507 1105 44-45 0.3138087777170355 1105 46-47 0.27154368480754343 1105 48-49 0.38691663912389274 1105 50-51 0.4285707401308336 1105 52-53 0.41671333248387654 1105 54-55 0.3966872266280994 1105 56-57 0.4543429049514103 1105 58-59 0.5561677728084291 1105 60-61 0.24984673499993804 1105 62-63 0.19652673646355368 1105 64-65 0.5321248397429095 1105 66-67 0.5247310643902132 1105 68-69 0.17240042650519527 1105 70-71 -0.2980409888949431 1105 72-73 -0.21152002516104673 1105 74-75 -0.3486008291512839 1105 76-77 -0.15589159483521797 1106 1 0.3328465200927795 1106 2 0.3159387994109366 1106 3 0.009062456053243295 1106 4 0.2749520964709333 1106 5 0.07392866249227126 1106 6 0.20338950917734877 1106 7 0.33203412542641075 1106 8 0.3590042433633016 1106 9 0.4184679394321478 1106 10-11 0.21071962670168176 1106 12-13 0.2081307922077329 1106 14-15 0.3094335699181343 1106 16-17 0.29663145888068954 1106 18-19 0.3785099323686154 1106 20-21 0.120234669034609 1106 22-23 0.2828141271760458 1106 24-25 0.3485914030813291 1106 26-27 0.38957138858771145 1106 28-29 0.3963236265246266 1106 30-31 0.43487317944016013 1106 32-33 0.3817855403332615 1106 34-35 0.4674768371408504 1106 36-37 0.3599682225022889 1106 38-39 0.3472816001686283 1106 40-41 0.48737282462113285 1106 42-43 0.4468045990118199 1106 44-45 0.21728940934148255 1106 46-47 0.4329197407408394 1106 48-49 0.4595165208546099 1106 50-51 0.25812939148107716 1106 52-53 0.29522845931461816 1106 54-55 0.37753983413320924 1106 56-57 0.42067819598170786 1106 58-59 0.29555286755446986 1106 60-61 0.2119949848283511 1106 62-63 0.17477480746311613 1106 64-65 0.30716444095220297 1106 66-67 0.39542616951450427 1106 68-69 0.10855840861281152 1106 70-71 -0.011747163358752033 1106 72-73 0.1978459280109668 1106 74-75 0.23598474152173665 1106 76-77 0.2847998054682961 1107 1 0.2085943144666942 1107 2 0.24803900192078032 1107 3 0.04239184093813009 1107 4 0.21115352201268678 1107 5 0.20959317497658247 1107 6 0.04740772367377133 1107 7 -0.07241061262061521 1107 8 0.1528241622226858 1107 9 0.23120009111635653 1107 10-11 0.34340708498670125 1107 12-13 0.27934785588329447 1107 14-15 0.3465174736883725 1107 16-17 0.2454995450145745 1107 18-19 0.2271465786779956 1107 20-21 0.1767689715601719 1107 22-23 0.3610579863895609 1107 24-25 -0.020544068944289506 1107 26-27 -0.026766987369882145 1107 28-29 0.2175507131847212 1107 30-31 0.4346688472328921 1107 32-33 0.5220264564340553 1107 34-35 0.5102764617345201 1107 36-37 0.4989757420186436 1107 38-39 0.36275936622760696 1107 40-41 0.3930872925715079 1107 42-43 0.3279441895729107 1107 44-45 0.2668573959289091 1107 46-47 0.3548396662062778 1107 48-49 0.5026877931586036 1107 50-51 0.3716989179633927 1107 52-53 0.4442440427776013 1107 54-55 0.40354013937864863 1107 56-57 0.4609822113259838 1107 58-59 0.36785799453885204 1107 60-61 0.3007375786400672 1107 62-63 0.48949663547325883 1107 64-65 0.535619580123619 1107 66-67 0.5972801938566867 1107 68-69 0.6099748385623975 1107 70-71 0.6448455686106946 1107 72-73 0.8246004628419819 1107 74-75 0.7670442621041609 1107 76-77 0.6244440122971362 1108 1 0.2592058266920958 1108 2 0.3339063946515921 1108 3 0.42449577485106715 1108 4 0.2208663897900749 1108 5 0.20107855007800168 1108 6 0.19490322258607762 1108 7 0.20603329860102093 1108 8 0.38615979413660995 1108 9 0.40666019332417846 1108 10-11 0.1994493170952225 1108 12-13 0.3639154551631236 1108 14-15 0.46786690570551315 1108 16-17 0.44611525933260765 1108 18-19 0.4488832496940063 1108 20-21 0.28498330845379627 1108 22-23 0.3289608943454141 1108 24-25 0.3342904386404797 1108 26-27 0.18570767886005513 1108 28-29 0.33656227324932786 1108 30-31 0.32184529956769126 1108 32-33 0.4042488570357037 1108 34-35 0.4083506103528407 1108 36-37 0.36037030882059895 1108 38-39 0.3118866777826099 1108 40-41 0.44338090032663047 1108 42-43 0.4381974033656846 1108 44-45 0.4037116339548277 1108 46-47 0.31635916445486956 1108 48-49 0.3218699150860935 1108 50-51 0.45883049410369736 1108 52-53 0.3236401050935598 1108 54-55 0.5268545884358176 1108 56-57 0.47683379772370316 1108 58-59 0.4178830993487388 1108 60-61 0.35765783261885886 1108 62-63 0.3659238115915784 1108 64-65 0.40576931469143673 1108 66-67 0.4490143651663061 1108 68-69 0.3672924596607814 1108 70-71 0.39879655928097435 1108 72-73 0.34872230628924683 1108 74-75 0.28052118130223036 1108 76-77 0.4143370097791106 1109 1 -0.025130029691155187 1109 2 -0.09254250787069651 1109 3 -0.21582663542930192 1109 4 -0.08524446542257635 1109 5 -0.07598488987243002 1109 6 -0.1655420625120314 1109 7 -0.1418144864616977 1109 8 0.09423758291257656 1109 9 0.004497034644266762 1109 10-11 -0.16156705713940056 1109 12-13 -0.04681997523086778 1109 14-15 0.13054509542536863 1109 16-17 0.1410478164769131 1109 18-19 0.014846141150592018 1109 20-21 0.053348487533718014 1109 22-23 0.11087818360758916 1109 24-25 0.07378450016283011 1109 26-27 0.15262866503826444 1109 28-29 0.2668820483200278 1109 30-31 0.3267695481790369 1109 32-33 0.24776983472026615 1109 34-35 0.2131407572821189 1109 36-37 0.2895625517215876 1109 38-39 0.3206243034587004 1109 40-41 0.4150198374006493 1109 42-43 0.39711263346195125 1109 44-45 0.2795795925275897 1109 46-47 0.11154887232772026 1109 48-49 0.2897657110555265 1109 50-51 0.3843112837518987 1109 52-53 0.38467183323574616 1109 54-55 0.4138876690285862 1109 56-57 0.4629752627478254 1109 58-59 0.4692345698937359 1109 60-61 0.47819591629874125 1109 62-63 0.5202875881301559 1109 64-65 0.5419727942571235 1109 66-67 0.5812234331187831 1109 68-69 0.5472107463824862 1109 70-71 0.4338313451375235 1109 72-73 0.36654302056173904 1109 74-75 0.30811890456819313 1109 76-77 0.4294234762351792 1110 1 -0.3824999093544008 1110 2 -0.5059673118061312 1110 3 -0.5467907439576507 1110 4 -0.38189557265692997 1110 5 -0.38547247637920634 1110 6 -0.624458350670622 1110 7 -0.4713078357211842 1110 8 -0.4424490438339319 1110 9 -0.35382642342646875 1110 10-11 -0.3607960532154877 1110 12-13 -0.4032644615862395 1110 14-15 -0.44241287315191613 1110 16-17 -0.5815278196524005 1110 18-19 -0.45881807896560645 1110 20-21 -0.6324114361883986 1110 22-23 -0.6094238370230656 1110 24-25 -0.45977221842100136 1110 26-27 -0.31397246341349216 1110 28-29 -0.3186326440799476 1110 30-31 -0.38407322821043977 1110 32-33 -0.27277757439849637 1110 34-35 -0.21249400992887502 1110 36-37 -0.06494690705115147 1110 38-39 -0.20663388357930046 1110 40-41 -0.20359385385201278 1110 42-43 -0.09083619329398118 1110 44-45 -0.178046939185883 1110 46-47 -0.2875028405195543 1110 48-49 -0.12136242637040695 1110 50-51 -0.19164310667984097 1110 52-53 -0.14041494858119918 1110 54-55 -0.1191108483723049 1110 56-57 -0.16292804873943112 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-0.766797959892699 2314 16-17 -1.4298002295989392 2314 18-19 -0.9509997292562673 2314 20-21 -0.7797381347120407 2314 22-23 -0.6051142377967196 2314 24-25 -0.5546436080418005 2314 26-27 -0.5830276906728216 2314 28-29 -0.6165349731033487 2314 30-31 -0.6838769344771265 2314 32-33 -0.8244927651147194 2314 34-35 -0.7968990927839457 2314 36-37 -0.8464658258638735 2314 38-39 -0.681928902669469 2314 40-41 -0.5885476510398959 2314 42-43 -0.5561845426322094 2314 44-45 -0.5768772101594308 2314 46-47 -0.543054359984108 2314 48-49 -0.5334969679114643 2314 50-51 -0.495831812368035 2314 52-53 -0.5363610201961002 2314 54-55 -0.5387470762668869 2314 56-57 -0.464591699252761 2314 58-59 -0.5111698831906679 2314 60-61 -0.48514945653566244 2314 62-63 -0.46458809991709415 2314 64-65 -0.5284236552429142 2314 66-67 -0.4436410325714064 2314 68-69 -0.2832485641803828 2314 70-71 -0.1465610536892612 2314 72-73 -0.10298511658416842 2314 74-75 -0.06191802923447298 2314 76-77 -0.10521485268871089 2315 1 -1.233639592843332 2315 2 -1.1700305697216749 2315 3 -1.1469466738335043 2315 4 -1.1266831071265102 2315 5 -1.0767689757092 2315 6 -1.1075264481710931 2315 7 -1.337189980199959 2315 8 -1.0997204924977808 2315 9 -1.1187324557420553 2315 10-11 -1.1121060635148368 2315 12-13 -1.1810637240202055 2315 14-15 -1.4555253796582193 2315 16-17 -1.3453835402189824 2315 18-19 -1.25277136645024 2315 20-21 -1.1706288741985205 2315 22-23 -1.2027919444906487 2315 24-25 -1.3064373346485638 2315 26-27 -1.2222675341245832 2315 28-29 -1.1151534587249543 2315 30-31 -1.1799495031697447 2315 32-33 -1.1643152777552004 2315 34-35 -1.0347596444460692 2315 36-37 -1.3205042345371112 2315 38-39 -1.1894058472104234 2315 40-41 -1.1325272624923883 2315 42-43 -1.3385274787187456 2315 44-45 -1.2097476601400672 2315 46-47 -1.166695061745063 2315 48-49 -0.9655687676663547 2315 50-51 -0.9816449893558961 2315 52-53 -1.0511076626248546 2315 54-55 -1.0580027273937596 2315 56-57 -1.1111688540818037 2315 58-59 -0.8441896365457033 2315 60-61 -1.08716113331511 2315 62-63 -1.20110885948095 2315 64-65 -1.19730097887172 2315 66-67 -1.1611336249170456 2315 68-69 -1.153863055381077 2315 70-71 -1.312743858884815 2315 72-73 -2.7174135388931937 2315 74-75 -3.366637910914765 2315 76-77 -3.0428572787106205 2316 1 -3.4514344386945126 2316 2 -3.4245336979044225 2316 3 -2.7727953722149294 2316 4 -2.7270128110470537 2316 5 -3.4313525053743206 2316 6 -2.9471091613629454 2316 7 -3.1800184749836475 2316 8 -3.1534012741407977 2316 9 -2.7664595231880824 2316 10-11 -2.8268255415414743 2316 12-13 -3.0019007448315946 2316 14-15 -2.888686030006987 2316 16-17 -3.9451909682124864 2316 18-19 -3.881012042303233 2316 20-21 -3.1618773930893056 2316 22-23 -3.2664977720630617 2316 24-25 -3.4060812455154874 2316 26-27 -3.046305797577155 2316 28-29 -3.10799074116375 2316 30-31 -3.3175063837702012 2316 32-33 -3.5025692530011554 2316 34-35 -3.429488831163056 2316 36-37 -3.3557625315295994 2316 38-39 -3.4157577778916206 2316 40-41 -3.324573211027431 2316 42-43 -3.5727518135377494 2316 44-45 -3.4379915395939094 2316 46-47 -3.157751765035119 2316 48-49 -3.4558608782208395 2316 50-51 -3.8295405128131534 2316 52-53 -4.771366033033193 2316 54-55 -3.893457200443688 2316 56-57 -3.7007783751835213 2316 58-59 -3.7355682572264612 2316 60-61 -3.5718708804041697 2316 62-63 -3.4965993908381954 2316 64-65 -3.3930177645388113 2316 66-67 -3.5101245573918156 2316 68-69 -3.440856880885388 2316 70-71 -3.124244741432353 2316 72-73 -3.156648127459629 2316 74-75 -3.2165581990964576 2316 76-77 -3.0949831570868085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82626.0 3 7525.0 4 9653.0 5 11267.0 6 12685.0 7 13707.0 8 15438.0 9 15751.0 10 19072.0 11 20727.0 12 16994.0 13 14663.0 14 15150.0 15 15612.0 16 15998.0 17 16978.0 18 17656.0 19 18908.0 20 20100.0 21 21866.0 22 24168.0 23 27508.0 24 32415.0 25 37380.0 26 43972.0 27 51440.0 28 60472.0 29 71694.0 30 84439.0 31 101194.0 32 123659.0 33 154607.0 34 204532.0 35 271837.0 36 376254.0 37 495190.0 38 427738.0 39 107052.0 40 757.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.028651043549754 26.4082456169416 28.303653829099463 22.259449510409183 2 22.690624564490207 26.629078686244206 28.185917566544273 22.49437918272131 3 22.814264190024605 26.818969454762343 27.77685012347909 22.589916231733966 4 22.44041115982976 26.73653174124388 28.369586439431373 22.453470659494986 5 22.71231171450253 26.68280975498196 28.151897928209817 22.452980602305686 6 22.81666715393436 26.509993860427038 28.138572435591332 22.534766550047262 7 22.62294059438749 26.361788763151377 28.177918610717846 22.83735203174329 8 22.644201577641557 26.739370769156302 27.757226038200354 22.859201615001794 9 22.542053682261646 26.613981324490787 28.06293652451522 22.781028468732345 10-11 23.232250052589727 26.609038611013876 27.601705287968365 22.557006048428036 12-13 23.324352407688867 26.22176892560743 27.636707344785844 22.81717132191786 14-15 22.835655614957975 26.52502665312733 27.776830327588215 22.86248740432648 16-17 22.982749432877313 26.345920557834006 27.98215092293157 22.689179086357107 18-19 23.10993861905545 26.338058459713192 27.568714885610014 22.98328803562135 20-21 22.908442966425536 26.637803712417895 27.57658072108003 22.87717260007654 22-23 23.109121320941707 26.624532800267964 27.2045435311 23.06180234769033 24-25 23.150827320559326 26.71370759782132 27.031452362082515 23.104012719536843 26-27 23.064773370041785 26.784525529516916 26.988102784096025 23.162598316345278 28-29 23.33802948211235 26.68703216499172 27.032973851102955 22.94196450179297 30-31 22.918210342250134 26.541171594138685 27.155496275525664 23.385121788085513 32-33 22.970240068504218 26.886168420392675 27.108397825393826 23.03519368570928 34-35 23.107003170560567 26.380038462538007 27.209159541568127 23.3037988253333 36-37 23.031188892265547 26.582066504706216 27.42453806430676 22.96220653872148 38-39 22.928179577532276 26.640276105198836 27.38215939136788 23.04938492590101 40-41 22.794424106891967 26.574550493889042 27.254352450993608 23.37667294822538 42-43 22.746281622130162 26.87333670324187 27.057143989521233 23.323237685106736 44-45 22.792365094437386 26.50966464097031 27.420773203763265 23.277197060829042 46-47 22.719597914849306 26.62326122376708 27.304525244846012 23.3526156165376 48-49 22.913799511625218 26.53895562131331 27.176357488261722 23.37088737879975 50-51 22.81131090563571 26.763216600323926 27.03364024670695 23.391832247333415 52-53 22.862081027540512 26.58523544147826 27.15589631726821 23.39678721371302 54-55 22.875356926390435 26.593548301154655 27.1408963524913 23.390198419963607 56-57 22.836072968794745 26.91251018703204 27.050032073820628 23.201384770352583 58-59 22.918979642456108 26.76800417647287 26.97913436622203 23.333881814848993 60-61 22.838960295536125 26.551517559559297 26.99423046996703 23.61529167493755 62-63 23.043783803277474 26.403342648068385 27.070514201315888 23.48235934733825 64-65 22.90805891688963 26.38780725138447 27.037017182197577 23.66711664952833 66-67 22.750976014223035 26.552704118234182 27.26627643426005 23.430043433282734 68-69 22.909817374411297 26.250281689202616 27.28977374365803 23.550127192728056 70-71 22.717698118364442 26.107134784656832 27.329758349811613 23.845408747167117 72-73 23.02030401571345 25.969550315644106 27.185200307793234 23.82494536084921 74-75 22.768538083937454 26.136536523520043 27.032102337375246 24.062823055167257 76-77 22.662913003341917 25.865441930786105 27.489634430709298 23.982010635162684 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6775.0 1 4133.0 2 1350.0 3 1294.0 4 1379.0 5 1448.5 6 1587.0 7 1760.5 8 1906.5 9 1948.0 10 2033.0 11 2215.0 12 2390.5 13 2469.0 14 2692.5 15 3121.0 16 3601.0 17 3876.0 18 4181.5 19 4935.5 20 6122.0 21 7749.0 22 8638.0 23 9975.0 24 13099.0 25 17262.5 26 19639.0 27 22247.5 28 29401.5 29 37337.5 30 40728.0 31 46697.0 32 58602.0 33 69975.5 34 80761.5 35 86110.0 36 91745.5 37 103082.0 38 110589.0 39 112395.0 40 116099.5 41 125042.5 42 136782.0 43 143283.0 44 142846.0 45 141158.0 46 143829.0 47 148559.5 48 149368.0 49 149892.5 50 150102.5 51 146160.0 52 142532.0 53 140605.5 54 132777.5 55 118118.5 56 101586.5 57 93812.0 58 89839.0 59 77759.0 60 64129.0 61 58606.0 62 51605.0 63 38490.0 64 28538.0 65 24700.0 66 21588.5 67 17095.5 68 13724.0 69 9810.0 70 7886.0 71 6837.5 72 5180.0 73 4197.5 74 3824.0 75 3233.0 76 2130.0 77 1281.0 78 944.0 79 788.0 80 519.5 81 351.5 82 247.0 83 198.0 84 169.0 85 135.5 86 108.5 87 86.0 88 74.0 89 67.0 90 60.5 91 49.0 92 56.0 93 56.5 94 45.0 95 41.0 96 42.0 97 45.5 98 49.0 99 107.5 100 166.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0096469790338989 2 0.01283015730097665 3 0.013967006682075848 4 0.015103856063175046 5 0.012342936137648423 6 0.009549534801233254 7 0.015915891335388756 8 0.017702368934258923 9 0.01737755482537344 10-11 0.0198623827583474 12-13 0.016029576273498675 14-15 0.007990427078582926 16-17 0.006674929937596714 18-19 0.017215147770930694 20-21 0.022412173513098453 22-23 0.02070689944144966 24-25 0.018985384664356588 26-27 0.023938799824860232 28-29 0.008932387994350833 30-31 0.008542611063688251 32-33 0.01083255053133092 34-35 0.01182323356343165 36-37 0.011709548625321729 38-39 0.014194376558295687 40-41 0.011725789330766003 42-43 0.014941449008732302 44-45 0.013025045766307941 46-47 0.023678948537751845 48-49 0.02338661583975491 50-51 0.03590819973729035 52-53 0.03896145236081391 54-55 0.027398070084490647 56-57 0.02041456674345272 58-59 0.015867169219055934 60-61 0.020041030518234415 62-63 0.024848279329739587 64-65 0.025627833191064753 66-67 0.027934013364151696 68-69 0.0411701883012352 70-71 0.05370801290421492 72-73 0.08422429843400621 74-75 0.08393196573600928 76-77 0.08950252770339534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3078684.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.62736889218156 #Duplication Level Percentage of deduplicated Percentage of total 1 73.73520589551121 35.85549057421984 2 13.169127249941095 12.80760017541932 3 5.141078654815302 7.499913847542724 4 2.521286490739292 4.904141130722137 5 1.4611116180547719 3.5525006821900837 6 0.8866769929359487 2.5870061534223976 7 0.5984962783946945 2.037230951706762 8 0.42105733374862614 1.6379928234362278 9 0.3110903893437163 1.361475640928635 >10 1.5393030613516907 13.604022399782782 >50 0.12911572834409912 4.360297092994889 >100 0.08087023361002707 7.272259407076895 >500 0.00403378786263821 1.2878064837905145 >1k 0.0014790555494037221 1.0047911320677094 >5k 6.722979770016919E-5 0.22747150469910926 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6958 0.22600565696252037 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 9.744423266564545E-5 3.248141088854848E-5 2 0.0 0.0 0.0 2.5985128710838786E-4 3.248141088854848E-5 3 0.0 0.0 0.0 5.521839851053242E-4 3.248141088854848E-5 4 0.0 0.0 0.0 0.0011693307919877455 3.248141088854848E-5 5 0.0 0.0 0.0 0.0021437731186442 6.496282177709697E-5 6 0.0 0.0 0.0 0.005099581509502112 6.496282177709697E-5 7 0.0 0.0 0.0 0.011823233563431648 6.496282177709697E-5 8 0.0 0.0 0.0 0.02039832603800845 1.624070544427424E-4 9 0.0 0.0 0.0 0.027544236433489116 1.624070544427424E-4 10 0.0 0.0 0.0 0.03897769306625818 1.948884653312909E-4 11 0.0 0.0 0.0 0.04992392853569902 1.948884653312909E-4 12 0.0 0.0 0.0 0.06574237563842213 1.948884653312909E-4 13 0.0 0.0 0.0 0.08708266259219849 1.948884653312909E-4 14 0.0 0.0 0.0 0.11329516117925711 1.948884653312909E-4 15 0.0 0.0 0.0 0.14366528036004994 2.2736987621983939E-4 16 0.0 0.0 0.0 0.17280110592707792 2.2736987621983939E-4 17 0.0 0.0 0.0 0.20518507258296076 2.2736987621983939E-4 18 0.0 0.0 0.0 0.2380237789912833 2.2736987621983939E-4 19 0.0 0.0 0.0 0.2730387399291386 2.5985128710838786E-4 20 0.0 0.0 0.0 0.31117191631229446 2.5985128710838786E-4 21 0.0 0.0 0.0 0.3503769792547725 2.5985128710838786E-4 22 0.0 0.0 0.0 0.3977998391520533 2.5985128710838786E-4 23 0.0 0.0 0.0 0.44619714137599054 2.5985128710838786E-4 24 0.0 0.0 0.0 0.4958287372136926 2.5985128710838786E-4 25 0.0 0.0 0.0 0.545005593298955 2.5985128710838786E-4 26 0.0 0.0 0.0 0.6035046143092309 2.5985128710838786E-4 27 0.0 0.0 0.0 0.6545004294042519 2.5985128710838786E-4 28 0.0 0.0 0.0 0.7087768669990164 2.5985128710838786E-4 29 0.0 0.0 0.0 0.7632806744700008 3.248141088854848E-4 30 0.0 0.0 0.0 0.8232738403811498 3.248141088854848E-4 31 0.0 0.0 0.0 0.889016216019572 3.572955197740333E-4 32 0.0 0.0 0.0 0.964048275172119 3.572955197740333E-4 33 0.0 0.0 0.0 1.0367416727406904 3.897769306625818E-4 34 0.0 0.0 0.0 1.1166459435265197 4.2225834155113027E-4 35 0.0 0.0 0.0 1.1971673611192315 4.2225834155113027E-4 36 0.0 0.0 0.0 1.2841200980678757 4.2225834155113027E-4 37 0.0 0.0 0.0 1.3752629370211429 4.2225834155113027E-4 38 0.0 0.0 0.0 1.4673802183010662 4.2225834155113027E-4 39 0.0 0.0 0.0 1.5589128341849958 4.2225834155113027E-4 40 0.0 0.0 0.0 1.6555125501675392 4.2225834155113027E-4 41 0.0 0.0 0.0 1.7614669124859843 4.2225834155113027E-4 42 0.0 0.0 0.0 1.871481451165498 4.2225834155113027E-4 43 0.0 0.0 0.0 1.988739344473158 4.2225834155113027E-4 44 0.0 0.0 0.0 2.118372655329355 4.2225834155113027E-4 45 0.0 0.0 0.0 2.2517088470268467 4.2225834155113027E-4 46 0.0 0.0 0.0 2.3931978728573635 4.5473975243967877E-4 47 0.0 0.0 0.0 2.5375777442569616 4.5473975243967877E-4 48 0.0 0.0 0.0 2.6885838234778237 5.197025742167757E-4 49 0.0 0.0 0.0 2.840629307847119 5.197025742167757E-4 50 3.248141088854848E-5 0.0 0.0 2.999690776968341 5.197025742167757E-4 51 3.248141088854848E-5 0.0 0.0 3.1680744110145764 5.197025742167757E-4 52 3.248141088854848E-5 0.0 0.0 3.341265293872317 5.521839851053242E-4 53 3.248141088854848E-5 0.0 0.0 3.53072936358522 5.521839851053242E-4 54 3.248141088854848E-5 0.0 0.0 3.723311648743424 5.521839851053242E-4 55 3.248141088854848E-5 0.0 0.0 3.921090959643796 5.521839851053242E-4 56 3.248141088854848E-5 0.0 0.0 4.13917115234951 5.521839851053242E-4 57 3.248141088854848E-5 0.0 0.0 4.353191168694156 5.521839851053242E-4 58 3.248141088854848E-5 0.0 0.0 4.573025357587852 5.521839851053242E-4 59 3.248141088854848E-5 0.0 0.0 4.79393143304087 5.521839851053242E-4 60 3.248141088854848E-5 0.0 0.0 5.0187352778005145 5.521839851053242E-4 61 3.248141088854848E-5 0.0 0.0 5.259162681197551 5.521839851053242E-4 62 3.248141088854848E-5 0.0 0.0 5.5074181046187265 5.521839851053242E-4 63 3.248141088854848E-5 0.0 0.0 5.76178003328695 5.846653959938727E-4 64 3.248141088854848E-5 0.0 0.0 6.027770307053274 5.846653959938727E-4 65 3.248141088854848E-5 0.0 0.0 6.306460812477019 5.846653959938727E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 440 0.0 21.777826 4 CTCGCGC 575 0.0 20.987654 36 CGCGGAA 530 0.0 20.763577 59 ATTCGCG 550 0.0 20.655315 56 TCGCGCT 615 0.0 20.201708 37 CGATCGT 595 0.0 19.684692 1 CGGTATA 150 1.32780515E-5 18.929426 10 CAATTCG 585 0.0 18.812668 54 AATTCGC 590 0.0 18.652328 55 CGCGCTT 670 0.0 18.012377 38 CCTCGCG 690 0.0 17.489996 35 TCGCGGA 635 0.0 16.770643 58 CGCTCCG 705 0.0 16.61494 25 GACGTAT 795 0.0 16.073719 39 AACTCGA 795 0.0 15.627736 13 GCGTAAG 960 0.0 15.529055 38 ACTCGAG 810 0.0 15.339082 14 ACGTATT 835 0.0 15.302727 40 GGGCGGT 735 0.0 14.969455 1 CGTATTA 880 0.0 14.923541 41 >>END_MODULE