FastQCFastQC Report
Thu 26 May 2016
SRR936165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078684
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80250.2606633223806016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56750.18433200679251266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41810.1358047789250212No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14400.047.947994100-101
GGTATCA83650.039.908551
CGTACAT18400.037.848100-101
CGGTATC10900.037.132767100-101
CCTGTCG9800.032.79417496-97
GTATCAA167800.032.697011
GCGTATC4100.032.664318100-101
GCGTACA7150.032.050114100-101
TCGAGTA5800.031.813292100-101
CGAGTAC15250.029.858398100-101
CGAGCCG6500.028.84205896-97
TCGTATC6850.028.674658100-101
AGTATCA36300.028.203093100-101
ACGTATC6050.028.039124100-101
CCGTACA9050.027.952154100-101
AGGTATC25150.027.926657100-101
AGCGTAC4250.027.30904498-99
CCGTATC7350.027.128912100-101
TACGCAG11250.026.983147100-101
CGTCCTA3700.026.543438100-101
Produced by FastQC (version 0.11.5)