FastQCFastQC Report
Thu 26 May 2016
SRR936164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082942
Sequences flagged as poor quality0
Sequence length77
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68590.22248229126594013No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA5000.027.68954559
CTCGCGC5400.027.60528236
ATTCGCG5100.026.45011556
CGCGCTT5900.024.66748638
TCGCGCT5950.024.45860737
CGATCGT6100.024.4358771
TCGCGGA5850.022.45262158
CAATTCG6050.022.29751654
CCTCGCG7150.021.83977335
AATTCGC6350.021.2440955
CGCTCCG7100.020.9986325
GACGTAT8000.020.85489339
ATCGTTC8000.019.5196113
ACGTATT8950.019.43448840
ACGCTCC7950.018.75501624
AACTCGA8050.018.5190313
GCGCCCT8050.018.51692831
CGGCCAA7750.018.3267450
GATCGTT8350.018.2763922
CGTATTA9400.018.12853841
Produced by FastQC (version 0.11.5)