##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936164_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3082942 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.38406236640196 39.0 36.0 41.0 29.0 41.0 2 36.250640135299335 39.0 36.0 41.0 29.0 41.0 3 36.11218861723639 39.0 36.0 41.0 28.0 41.0 4 35.9979315212547 39.0 35.0 41.0 28.0 41.0 5 35.92351169759275 39.0 35.0 41.0 28.0 41.0 6 35.82727732146761 39.0 35.0 40.0 27.0 41.0 7 35.679156792440466 39.0 35.0 40.0 27.0 41.0 8 35.55824566274682 38.0 35.0 40.0 27.0 41.0 9 35.71483537478162 39.0 35.0 40.0 27.0 41.0 10-11 35.92254800771471 39.0 35.0 41.0 28.5 41.0 12-13 35.826181777016885 39.0 35.0 41.0 27.5 41.0 14-15 35.66061022231362 39.0 35.0 41.0 27.0 41.0 16-17 35.5030235729378 39.0 35.0 41.0 26.5 41.0 18-19 35.33144071474585 39.0 35.0 41.0 26.0 41.0 20-21 35.11695387068586 39.0 34.5 41.0 25.0 41.0 22-23 34.83435319250248 39.0 34.0 41.0 24.0 41.0 24-25 34.59889190260472 38.5 34.0 40.5 23.5 41.0 26-27 33.63485544003099 37.5 33.0 39.5 22.0 40.5 28-29 33.631989670905256 38.0 33.0 39.5 21.5 40.5 30-31 34.02866644912554 38.0 33.0 40.0 22.0 41.0 32-33 33.890819872706004 38.0 33.0 40.0 21.5 41.0 34-35 33.6298701045949 38.0 33.0 40.0 20.0 41.0 36-37 33.31406023856433 37.0 33.0 40.0 18.5 41.0 38-39 32.99763505119461 37.0 32.0 40.0 17.5 41.0 40-41 32.63681103958491 36.0 32.0 39.5 13.5 41.0 42-43 32.260356827990925 36.0 31.5 39.0 12.0 41.0 44-45 31.892101765132136 35.0 31.0 39.0 8.0 40.5 46-47 31.470688550092735 35.0 31.0 38.0 7.0 40.0 48-49 31.057353171094363 35.0 31.0 37.0 2.0 39.5 50-51 30.601229766891493 35.0 30.0 37.0 2.0 39.0 52-53 30.112032435251784 35.0 30.0 36.0 2.0 39.0 54-55 29.649754682378067 34.0 29.0 36.0 2.0 38.5 56-57 29.216327293864104 34.0 29.0 35.0 2.0 37.0 58-59 28.7858910417387 34.0 29.0 35.0 2.0 37.0 60-61 28.42423762756484 34.0 29.0 35.0 2.0 36.0 62-63 28.050821585355806 34.0 27.5 35.0 2.0 36.0 64-65 27.664691713305018 34.0 27.0 35.0 2.0 35.5 66-67 27.307945786849054 33.5 26.0 35.0 2.0 35.0 68-69 26.94097667098505 33.0 25.5 35.0 2.0 35.0 70-71 26.477256951314686 33.0 25.0 35.0 2.0 35.0 72-73 25.97949182955761 33.0 24.0 35.0 2.0 35.0 74-75 25.390043503899847 33.0 21.0 35.0 2.0 35.0 76-77 23.61616355416352 30.5 8.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.0259375249449434 1101 2 0.9776399946548509 1101 3 1.0365717120658857 1101 4 0.987737687633178 1101 5 1.022016249472884 1101 6 0.9564158709914281 1101 7 0.9881552541077099 1101 8 0.8825575039262858 1101 9 0.9576162172196518 1101 10-11 1.0291619167889365 1101 12-13 1.2149615767298485 1101 14-15 1.2370826924761502 1101 16-17 1.1332783118710381 1101 18-19 1.0869841549682775 1101 20-21 1.1802418464882223 1101 22-23 1.0428470341096272 1101 24-25 1.1497065205487544 1101 26-27 1.169886085178831 1101 28-29 1.201388585809262 1101 30-31 1.2125666402595385 1101 32-33 1.3257890591240056 1101 34-35 1.3270924917906513 1101 36-37 1.3074068919011665 1101 38-39 1.2254459197806824 1101 40-41 1.2142631095165441 1101 42-43 1.3628849972108057 1101 44-45 1.309596581098905 1101 46-47 1.3275320571845732 1101 48-49 1.3097632723921144 1101 50-51 1.240078838689179 1101 52-53 1.4684724371950963 1101 54-55 1.338941791516973 1101 56-57 1.4106972326459726 1101 58-59 1.5118405944156628 1101 60-61 1.3730448262282202 1101 62-63 1.3338889782447687 1101 64-65 1.2961253578941232 1101 66-67 1.5378674675424726 1101 68-69 1.3621904559846278 1101 70-71 1.2329456707465276 1101 72-73 0.8363455066012513 1101 74-75 0.9532651127468732 1101 76-77 1.113346184544696 1102 1 0.7611547253426849 1102 2 0.7299504511199828 1102 3 0.7372554129546955 1102 4 0.6150778954831395 1102 5 0.599529584086369 1102 6 0.6937115710142976 1102 7 0.49738815149509463 1102 8 0.5059606220426502 1102 9 0.566788082989234 1102 10-11 0.8076469261271413 1102 12-13 0.842134650900995 1102 14-15 0.7429293746922099 1102 16-17 0.781081907477251 1102 18-19 0.843565723908938 1102 20-21 0.8752253288129026 1102 22-23 0.6687407070021649 1102 24-25 0.7673878939191567 1102 26-27 0.5702938595550933 1102 28-29 0.7409372576071505 1102 30-31 0.8675939210257724 1102 32-33 0.8600797163941678 1102 34-35 0.7243310862777506 1102 36-37 0.7057970148083257 1102 38-39 0.7847230926102355 1102 40-41 0.7693428603509886 1102 42-43 0.7263551277988967 1102 44-45 0.6522667327239482 1102 46-47 0.8100545710023006 1102 48-49 0.7178122297788398 1102 50-51 0.76903644556484 1102 52-53 0.736152404025205 1102 54-55 0.8762716154303583 1102 56-57 0.8270189707873072 1102 58-59 0.9647346427933101 1102 60-61 0.8359142418046126 1102 62-63 0.7387746876731107 1102 64-65 0.7549683990054596 1102 66-67 0.8106774537339163 1102 68-69 0.6431857116630759 1102 70-71 0.5931807586675255 1102 72-73 0.7234101670930002 1102 74-75 0.7488134738828194 1102 76-77 0.8387605941218865 1103 1 0.45261910777821157 1103 2 0.3356514203998273 1103 3 0.4478772775639399 1103 4 0.5203646284133896 1103 5 0.4108654488640653 1103 6 0.2767352353926995 1103 7 0.405563744053552 1103 8 0.2881122785728607 1103 9 0.4214172216457186 1103 10-11 0.5196316069741655 1103 12-13 0.5107615262042344 1103 14-15 0.4170484403591175 1103 16-17 0.31760261870160633 1103 18-19 0.40347050411574514 1103 20-21 0.37484335829580573 1103 22-23 0.3565113633627419 1103 24-25 0.47051785760823606 1103 26-27 0.4145883825486436 1103 28-29 0.4123515093779133 1103 30-31 0.6182236978814473 1103 32-33 0.5366016375872391 1103 34-35 0.7009697338648735 1103 36-37 0.5796974557351575 1103 38-39 0.586595875075659 1103 40-41 0.6470961619641642 1103 42-43 0.6121790393905187 1103 44-45 0.6325461999106672 1103 46-47 0.610676933833961 1103 48-49 0.5524252236566376 1103 50-51 0.38897133264035944 1103 52-53 0.5136475789783788 1103 54-55 0.5068149625597087 1103 56-57 0.5601702156909099 1103 58-59 0.5014880357472578 1103 60-61 0.4159028420012909 1103 62-63 0.47038482388949276 1103 64-65 0.42898876918471274 1103 66-67 0.313269752401645 1103 68-69 0.26426917933852323 1103 70-71 0.14407613551940202 1103 72-73 0.19916761344215672 1103 74-75 0.1992175085353729 1103 76-77 0.3798787119799556 1104 1 0.4304912947332582 1104 2 0.512239802221309 1104 3 0.5976463289797067 1104 4 0.5413224984907643 1104 5 0.4359936589526541 1104 6 0.29016839157640106 1104 7 0.32178477299535757 1104 8 0.32268155992387904 1104 9 0.3302351370652872 1104 10-11 0.26254101561866605 1104 12-13 0.4472400715690057 1104 14-15 0.42170610307100276 1104 16-17 0.4592227179533026 1104 18-19 0.3492642597524309 1104 20-21 0.34259553858125713 1104 22-23 0.5186773193120899 1104 24-25 0.4598461583443765 1104 26-27 0.31257304063511526 1104 28-29 0.47340790799866994 1104 30-31 0.5446300246013749 1104 32-33 0.6483726456635281 1104 34-35 0.7150572981080927 1104 36-37 0.692062762596457 1104 38-39 0.6941869855357723 1104 40-41 0.7169773390557594 1104 42-43 0.8220850017699419 1104 44-45 0.5755940441295344 1104 46-47 0.5818381851386327 1104 48-49 0.6680123646015979 1104 50-51 0.5119489204022045 1104 52-53 0.5012451471551955 1104 54-55 0.44333853230443054 1104 56-57 0.459833442541008 1104 58-59 0.4954240232850644 1104 60-61 0.4758256856444021 1104 62-63 0.42536792880758156 1104 64-65 0.4601575007546579 1104 66-67 0.49603810461527686 1104 68-69 0.36266311019028663 1104 70-71 0.4778549914257262 1104 72-73 0.45031809574423676 1104 74-75 0.4020364492671682 1104 76-77 0.5435030663234386 1105 1 0.10546044850455871 1105 2 0.2295073933112377 1105 3 0.21509807866564756 1105 4 0.23547771518972382 1105 5 0.22128945333324168 1105 6 0.2127711365210061 1105 7 0.2673867259997351 1105 8 0.2670262461931969 1105 9 0.22987912251008424 1105 10-11 0.4247502733864721 1105 12-13 0.5301211877610186 1105 14-15 0.5998972479512474 1105 16-17 0.6074959902171599 1105 18-19 0.454431692140588 1105 20-21 0.3173669537968138 1105 22-23 0.3372575165934961 1105 24-25 0.5063552235277342 1105 26-27 0.3011294595414711 1105 28-29 0.33816389001575686 1105 30-31 0.41547076976917197 1105 32-33 0.5767239040955445 1105 34-35 0.45684271099040785 1105 36-37 0.5475757444553295 1105 38-39 0.5914751504888116 1105 40-41 0.599691137146408 1105 42-43 0.5314603056943739 1105 44-45 0.34066337363359267 1105 46-47 0.3432815981634718 1105 48-49 0.22174273024313607 1105 50-51 0.18775348819869464 1105 52-53 0.2695039744651666 1105 54-55 0.13743229184233385 1105 56-57 0.3302150237233974 1105 58-59 0.2654287493799643 1105 60-61 0.3593750098061115 1105 62-63 0.17346835910855063 1105 64-65 0.276163668207662 1105 66-67 0.0038204747798822325 1105 68-69 0.05158897795195827 1105 70-71 -0.15798679128685222 1105 72-73 -0.609214285288818 1105 74-75 -0.8500065391486338 1105 76-77 -0.8016722832260683 1106 1 0.1932462452832837 1106 2 0.1068913767915518 1106 3 0.02516485033564919 1106 4 0.3290576504671989 1106 5 0.14776008610229496 1106 6 0.16572410025200668 1106 7 -0.1587715997880963 1106 8 0.004331716068556091 1106 9 0.017444986532666462 1106 10-11 -0.17455459823231223 1106 12-13 0.2921462963674699 1106 14-15 0.2633946913409133 1106 16-17 0.2121938072760443 1106 18-19 0.34766218456272924 1106 20-21 0.2020006203435898 1106 22-23 0.2462755222770454 1106 24-25 0.15027086778018628 1106 26-27 0.09702414529806447 1106 28-29 0.2775660731026832 1106 30-31 0.42284573453719787 1106 32-33 0.25610397165344523 1106 34-35 0.20284785662506266 1106 36-37 0.2207862770461304 1106 38-39 0.27209777250584466 1106 40-41 0.37200544011075465 1106 42-43 0.3116300629607949 1106 44-45 0.24202882080570376 1106 46-47 0.33811168107034817 1106 48-49 0.27070191072808214 1106 50-51 0.22447487840610592 1106 52-53 0.20822540796598332 1106 54-55 0.1875112125847771 1106 56-57 0.26134945569986456 1106 58-59 0.015812417581077653 1106 60-61 -0.2460084918387615 1106 62-63 -0.2355979310624896 1106 64-65 -0.019116497904438035 1106 66-67 -0.11878942906949774 1106 68-69 -0.05276983826270154 1106 70-71 -0.10566070713186804 1106 72-73 -0.16353225877149313 1106 74-75 0.020123947856223623 1106 76-77 -0.047206472849175896 1107 1 0.19136251894271794 1107 2 0.19171689772435485 1107 3 0.24187649784096976 1107 4 0.19062362168872227 1107 5 0.1800919266331391 1107 6 -0.052001569295512695 1107 7 0.006050982705232855 1107 8 0.09392840331442187 1107 9 0.009891743051056778 1107 10-11 0.0822647949473847 1107 12-13 0.155948324541761 1107 14-15 -0.05278823237598829 1107 16-17 -0.12200512507648398 1107 18-19 0.018010617510753946 1107 20-21 -0.03038843728976559 1107 22-23 0.018188001486613814 1107 24-25 0.016604424498588344 1107 26-27 -0.10207080883276376 1107 28-29 -0.016123682377489956 1107 30-31 0.22388663440281675 1107 32-33 0.2831698421780757 1107 34-35 0.13113094423483318 1107 36-37 0.18551474917913424 1107 38-39 0.28279730556741356 1107 40-41 0.2023990499380801 1107 42-43 0.17978196899670706 1107 44-45 0.12524820461017683 1107 46-47 0.24403392787843003 1107 48-49 0.27337591235312075 1107 50-51 0.2775606955961081 1107 52-53 0.25395991800616 1107 54-55 0.12689450488702292 1107 56-57 0.134979739248422 1107 58-59 0.0766230319015655 1107 60-61 0.14851563721993344 1107 62-63 0.08965145650943285 1107 64-65 0.12089786442381367 1107 66-67 0.27522937086661514 1107 68-69 0.1840222126725628 1107 70-71 0.12175215533353878 1107 72-73 0.08174686910661322 1107 74-75 -0.02289218013994443 1107 76-77 0.08987270529057056 1108 1 0.36030533039862433 1108 2 0.2351165382646485 1108 3 0.3733401098584963 1108 4 0.21627697057913764 1108 5 0.34100580255778823 1108 6 0.3746597943112988 1108 7 0.29649832478346383 1108 8 0.3595812321908056 1108 9 0.3659425126718361 1108 10-11 0.35542410774882427 1108 12-13 0.4642833370871031 1108 14-15 0.47354682242049506 1108 16-17 0.3744987961543913 1108 18-19 0.419231919235898 1108 20-21 0.40662059632914094 1108 22-23 0.3807188366941645 1108 24-25 0.4700192916999981 1108 26-27 0.4396678337355837 1108 28-29 0.5363420779480421 1108 30-31 0.6646921767774643 1108 32-33 0.6631518204414917 1108 34-35 0.6228931905654846 1108 36-37 0.5534516991017853 1108 38-39 0.6060800810773515 1108 40-41 0.3833617015546764 1108 42-43 0.41741315066360585 1108 44-45 0.4160238055513936 1108 46-47 0.3165269320645443 1108 48-49 0.29537128747825037 1108 50-51 0.18923834214256274 1108 52-53 0.42906367705765547 1108 54-55 0.5281706518429914 1108 56-57 0.5147715873783199 1108 58-59 0.4505387213751817 1108 60-61 0.5126725319560883 1108 62-63 0.4406083229698261 1108 64-65 0.454577467523098 1108 66-67 0.5670332183204962 1108 68-69 0.6287502047033477 1108 70-71 0.615845933365545 1108 72-73 0.6076153331801457 1108 74-75 0.6132747151162299 1108 76-77 0.5564102080531441 1109 1 -0.0017769852634827998 1109 2 -0.10636082010799441 1109 3 -0.1530054731113779 1109 4 -0.03543504551895893 1109 5 -0.03317490317193261 1109 6 -0.15787862188385304 1109 7 -0.0616765010226743 1109 8 -0.18171548826282446 1109 9 -0.09428650777799419 1109 10-11 -0.15299284517298162 1109 12-13 -0.05222617009653874 1109 14-15 0.12557791648906402 1109 16-17 0.21198333960246174 1109 18-19 0.052212596043943904 1109 20-21 0.16821112096751278 1109 22-23 0.21757674385150239 1109 24-25 0.1936588496068694 1109 26-27 0.051687291731951746 1109 28-29 0.25429084695903725 1109 30-31 0.44187860229945386 1109 32-33 0.3866033670362299 1109 34-35 0.4483183555272845 1109 36-37 0.414369947984234 1109 38-39 0.3733825854574704 1109 40-41 0.39838110930576676 1109 42-43 0.4948721772695137 1109 44-45 0.5127328008050576 1109 46-47 0.45099474669294537 1109 48-49 0.47318403270484666 1109 50-51 0.3539421747955416 1109 52-53 0.39172075994725475 1109 54-55 0.5420752679138836 1109 56-57 0.44691691593549265 1109 58-59 0.4940946413111611 1109 60-61 0.4095210089317014 1109 62-63 0.2100972630843927 1109 64-65 0.24116578627100083 1109 66-67 0.2861945632764211 1109 68-69 0.31994080752827614 1109 70-71 0.3100202583528535 1109 72-73 0.16292967052860874 1109 74-75 0.22039244961770166 1109 76-77 0.38082209314585924 1110 1 -0.21727989646486634 1110 2 -0.21215868991087916 1110 3 -0.3365684291601738 1110 4 -0.3438038656355431 1110 5 -0.27004909388674747 1110 6 -0.21730799500919318 1110 7 -0.3723502993501313 1110 8 -0.5260121448692772 1110 9 -0.4910114924836506 1110 10-11 -0.37976151615156795 1110 12-13 -0.2851976397529299 1110 14-15 -0.20661567927794522 1110 16-17 -0.27305070400660014 1110 18-19 -0.3618089263538167 1110 20-21 -0.41969473926982204 1110 22-23 -0.3583763236837427 1110 24-25 -0.5582198021463967 1110 26-27 -0.5661377797541718 1110 28-29 -0.3284005838966664 1110 30-31 -0.256727619471512 1110 32-33 -0.30625326075875137 1110 34-35 -0.35912145245754346 1110 36-37 -0.21452265935347015 1110 38-39 -0.22067076678214192 1110 40-41 -0.2398860295378853 1110 42-43 -0.23715442834178901 1110 44-45 -0.14342164703679927 1110 46-47 -0.21114262328761058 1110 48-49 -0.33686208986214794 1110 50-51 -0.2548222030648617 1110 52-53 -0.2737382109505333 1110 54-55 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-0.6101384511437331 2314 30-31 -0.7943364757523597 2314 32-33 -0.9058851856564019 2314 34-35 -0.9545612004135009 2314 36-37 -1.3606302708935374 2314 38-39 -0.9209905048339877 2314 40-41 -0.7877656788818364 2314 42-43 -0.7650977051493513 2314 44-45 -0.7189668288537554 2314 46-47 -0.514402866663854 2314 48-49 -0.42368295089682917 2314 50-51 -0.4162711872545515 2314 52-53 -0.37625567129448 2314 54-55 -0.5014529541857691 2314 56-57 -0.6046528754302187 2314 58-59 -0.45196293229743034 2314 60-61 -0.4376060014606189 2314 62-63 -0.47056007063416416 2314 64-65 -0.3769187705890644 2314 66-67 -0.3209220285340493 2314 68-69 -0.33613655263071607 2314 70-71 -0.3065168326740384 2314 72-73 -0.2232254350142533 2314 74-75 -0.20888157659509687 2314 76-77 -0.23475850202657256 2315 1 -1.1688684501982962 2315 2 -1.0096268725342128 2315 3 -0.8164835839677735 2315 4 -0.9083792625570695 2315 5 -1.0484130200420196 2315 6 -1.0536905598238278 2315 7 -1.4506968042707697 2315 8 -1.307128591734596 2315 9 -0.9384347561543791 2315 10-11 -1.0334302494852068 2315 12-13 -0.9229170094341086 2315 14-15 -0.887463471462894 2315 16-17 -0.8908759772700066 2315 18-19 -0.828292678647216 2315 20-21 -0.7615441184364613 2315 22-23 -0.9463753780577591 2315 24-25 -1.0195397218162938 2315 26-27 -0.9119191779645917 2315 28-29 -0.8237872640055173 2315 30-31 -0.9619327115575089 2315 32-33 -0.8332521799206063 2315 34-35 -0.9949320028878148 2315 36-37 -1.0247088157257167 2315 38-39 -1.060798424572745 2315 40-41 -1.211392603820471 2315 42-43 -1.160726750432584 2315 44-45 -1.1243315646501664 2315 46-47 -0.9833981216715095 2315 48-49 -0.9623561425034843 2315 50-51 -0.8819030655980953 2315 52-53 -0.9363520782189347 2315 54-55 -1.0597298948857947 2315 56-57 -1.0835303850136668 2315 58-59 -0.8667643218782146 2315 60-61 -1.0891280730876254 2315 62-63 -0.9928707275143402 2315 64-65 -0.8221259108832548 2315 66-67 -0.909584845553649 2315 68-69 -1.1506457656016487 2315 70-71 -1.1487964824304306 2315 72-73 -1.0701327491288168 2315 74-75 -1.0770777428475462 2315 76-77 -1.1273892763053297 2316 1 -2.1291478830845563 2316 2 -1.7808171403435793 2316 3 -2.012790226990276 2316 4 -2.7852200987377387 2316 5 -2.2743853506672664 2316 6 -2.161101712191737 2316 7 -1.7969542395306135 2316 8 -1.6079405070901203 2316 9 -1.9056188043876574 2316 10-11 -1.7713390901319954 2316 12-13 -2.445578927920799 2316 14-15 -3.6773931652037533 2316 16-17 -2.735892399374343 2316 18-19 -2.2520100820047517 2316 20-21 -2.0041689363575586 2316 22-23 -2.451373797197924 2316 24-25 -2.309584097901066 2316 26-27 -2.2807832907152843 2316 28-29 -2.8642963517857503 2316 30-31 -3.0307679564329533 2316 32-33 -3.0657955160999997 2316 34-35 -2.8953220501405 2316 36-37 -2.7860296559387194 2316 38-39 -2.9659591809619883 2316 40-41 -2.610508544233511 2316 42-43 -2.8816357473833847 2316 44-45 -2.8020586496922775 2316 46-47 -2.7183991730323953 2316 48-49 -2.49288786636367 2316 50-51 -2.5355043457636235 2316 52-53 -2.5370784336725976 2316 54-55 -2.584388126226937 2316 56-57 -2.44707112410255 2316 58-59 -2.618398768024864 2316 60-61 -2.408785314163911 2316 62-63 -2.339484683579169 2316 64-65 -2.30159891687423 2316 66-67 -2.362064478936727 2316 68-69 -2.5228131371637907 2316 70-71 -2.4298262662370327 2316 72-73 -2.4994468036091675 2316 74-75 -2.804199951713496 2316 76-77 -3.158824585026128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82353.0 3 7689.0 4 9802.0 5 12010.0 6 13276.0 7 13837.0 8 15733.0 9 16423.0 10 19249.0 11 20782.0 12 16864.0 13 14954.0 14 15398.0 15 16169.0 16 16264.0 17 17271.0 18 17868.0 19 19048.0 20 20625.0 21 22298.0 22 24751.0 23 27892.0 24 32420.0 25 37779.0 26 44442.0 27 51831.0 28 60999.0 29 71248.0 30 84424.0 31 101141.0 32 121950.0 33 153581.0 34 201617.0 35 269679.0 36 377093.0 37 497369.0 38 430844.0 39 105324.0 40 645.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.020815898088642 26.401112482143063 28.29988361365963 22.278188006108664 2 22.665376876092193 26.572962213612804 28.228247516520575 22.533413393774424 3 22.818972654110965 26.77367429403384 27.76080067891094 22.646552372944257 4 22.451627973371053 26.726534214043784 28.34600760456274 22.475830208022423 5 22.79727611854354 26.643683254539397 28.077999995458253 22.48104063145881 6 22.790444182032882 26.496535302285146 28.135908282833526 22.57711223284845 7 22.640353150981 26.343448866978264 28.138466484623613 22.877731497417127 8 22.600348105937105 26.685385876891182 27.818530720430058 22.895735296741655 9 22.543405858647333 26.5618744764662 28.122564817620454 22.77215484726601 10-11 23.19586416433685 26.630460260955395 27.607741228016813 22.565934346690934 12-13 23.35471867061606 26.186050812210244 27.594303659893654 22.864926857280043 14-15 22.791003585363026 26.50643232454956 27.81437210731922 22.8881919827682 16-17 23.018280543221273 26.348414901783578 27.937040802904285 22.69626375209086 18-19 23.142350331443357 26.33805211092793 27.563682086876636 22.95591547075208 20-21 22.947263759060924 26.607171531869962 27.5648193654393 22.88074534362982 22-23 23.123832930847218 26.62216935041207 27.195047020153716 23.058950698586994 24-25 23.156832807963983 26.7046980867385 27.05619159348481 23.0822775118127 26-27 23.078239444216003 26.764720433220845 26.978897758875327 23.17814236368783 28-29 23.311262660684413 26.69652279044842 27.04675455359683 22.94545999527034 30-31 22.939488008268114 26.541557177481394 27.161422350277913 23.35753246397258 32-33 22.9603769113724 26.90943412201479 27.089993964578024 23.040195002034785 34-35 23.079712930549835 26.372616094406055 27.200115876245984 23.347555098798125 36-37 23.00083289336775 26.62134818430057 27.41163179787373 22.966187124457953 38-39 22.884802736011665 26.630451885079466 27.41359694242521 23.07114843648366 40-41 22.813463842059463 26.53364379033285 27.233923834225042 23.418968533382643 42-43 22.75822948743403 26.858585308059947 27.042837974443724 23.3403472300623 44-45 22.79660773629612 26.51866674517777 27.387090406024072 23.297635112502032 46-47 22.707205836456964 26.588055221582536 27.297384715505814 23.407354226454686 48-49 22.91804709814582 26.545873741958893 27.16004646197811 23.376032697917175 50-51 22.779371277646582 26.76337329961866 27.04939215113398 23.40786327160078 52-53 22.80497342893951 26.60722185801286 27.204038522156825 23.383766190890807 54-55 22.891419677892994 26.553460876403978 27.167410540837324 23.387708904865704 56-57 22.83224167631802 26.88008318004717 27.04897371263954 23.238701430995274 58-59 22.927429685306354 26.707798977456115 26.985893021062573 23.378878316174955 60-61 22.834138710959976 26.528099214174862 26.99656409342175 23.641197981443412 62-63 23.031370851085263 26.388251938200163 27.075931569785183 23.50444564092939 64-65 22.90545078204334 26.403703715719708 27.03657321943607 23.654272282800886 66-67 22.758109878886724 26.539481124775776 27.227761157906656 23.474647838430844 68-69 22.88019610674255 26.21464343712433 27.32441162299095 23.58074883314217 70-71 22.719651801277998 26.115293605246475 27.330652801203676 23.83440179227185 72-73 22.97968954129036 26.029309379488303 27.166467862736027 23.824533216485307 74-75 22.79569477106499 26.138231459373145 27.022870209971856 24.043203559590008 76-77 22.7359883920978 25.798919712009766 27.485668655417996 23.979423240474443 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6622.0 1 4006.5 2 1309.5 3 1304.5 4 1381.0 5 1428.5 6 1575.5 7 1719.0 8 1853.0 9 1943.0 10 1990.5 11 2145.5 12 2353.5 13 2454.0 14 2642.5 15 3041.5 16 3537.5 17 3823.0 18 4074.0 19 4905.5 20 5985.5 21 7637.0 22 8789.0 23 10072.5 24 13098.0 25 17218.0 26 19596.0 27 22242.0 28 29755.5 29 37734.5 30 40846.0 31 46640.5 32 58492.5 33 70003.0 34 80321.0 35 85186.0 36 91324.0 37 103094.5 38 110793.5 39 112860.0 40 116935.5 41 125761.5 42 136756.5 43 143001.0 44 142274.5 45 141108.5 46 144549.5 47 149096.0 48 149762.0 49 150388.0 50 150267.5 51 146305.5 52 143090.0 53 141337.5 54 133198.0 55 118138.5 56 101915.0 57 94364.0 58 90255.0 59 77829.0 60 64240.0 61 58968.0 62 51765.5 63 38521.5 64 28681.0 65 24882.0 66 21819.0 67 17185.0 68 13635.0 69 9726.5 70 7797.0 71 6836.5 72 5190.5 73 4212.0 74 3919.0 75 3266.5 76 2104.0 77 1241.0 78 888.0 79 764.0 80 532.5 81 350.0 82 238.5 83 202.0 84 175.5 85 122.5 86 86.5 87 77.0 88 66.5 89 67.0 90 61.5 91 45.0 92 40.0 93 36.0 94 32.5 95 31.0 96 34.0 97 31.5 98 34.5 99 71.0 100 102.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034382742198847726 2 0.008790304845177106 3 0.011612284629422156 4 0.01887807166012205 5 0.013915279625760071 6 0.013688223781050697 7 0.0168994421562261 8 0.017742792436575193 9 0.01699675180395869 10-11 0.019932259510558423 12-13 0.01985116813744793 14-15 0.008741650021310814 16-17 0.005952106786309959 18-19 0.011806903924887332 20-21 0.01093111709529404 22-23 0.01407746237198105 24-25 0.021602741796634514 26-27 0.014580228885266087 28-29 0.008725431746688715 30-31 0.00828753833189207 32-33 0.00997423889259026 34-35 0.011125736390759217 36-37 0.01020129473729963 38-39 0.01618583807285379 40-41 0.012634035930614329 42-43 0.015893909129656023 44-45 0.018667234090034776 46-47 0.01699675180395869 48-49 0.013672005506428601 50-51 0.026030330768467263 52-53 0.029955153227014975 54-55 0.024895051544920405 56-57 0.015310051243260495 58-59 0.01724002592329016 60-61 0.020775609790907517 62-63 0.024830178446432014 64-65 0.02049989912233185 66-67 0.020143097080645694 68-69 0.03071741213425358 70-71 0.055563808855307685 72-73 0.06542451982554326 74-75 0.07663134758941297 76-77 0.0740202053752552 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3082942.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.67596287342572 #Duplication Level Percentage of deduplicated Percentage of total 1 73.88806701043146 35.965728065889536 2 13.06066841219629 12.71481221468381 3 5.122411638828369 7.480149562620405 4 2.498805667790177 4.865270876530416 5 1.4306891317482 3.4820085530194533 6 0.8917706025440157 2.6044675644626993 7 0.6011639295867641 2.048356318218563 8 0.4058595345576895 1.5804482908764752 9 0.30811020840582864 1.3497804958757018 >10 1.5759888378124451 13.735657904657389 >50 0.12951286179600552 4.357674569841017 >100 0.08153098865217671 7.337608045303175 >500 0.003881829478475772 1.2571809452211293 >1k 0.0014724180778052378 0.9974048150813338 >5k 6.692809444569263E-5 0.22345177771893351 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6859 0.22248229126594013 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.487309848839194E-5 0.0 2 0.0 0.0 0.0 2.5949239395356774E-4 0.0 3 0.0 0.0 0.0 5.189847879071355E-4 3.243654924419597E-5 4 0.0 0.0 0.0 0.0013298985190120347 3.243654924419597E-5 5 0.0 0.0 0.0 0.0026922335872682653 3.243654924419597E-5 6 0.0 0.0 0.0 0.005935888511687862 3.243654924419597E-5 7 0.0 0.0 0.0 0.012390761811282859 3.243654924419597E-5 8 0.0 0.0 0.0 0.019883604686692126 9.73096477325879E-5 9 0.0 0.0 0.0 0.02880365572884602 9.73096477325879E-5 10 0.0 0.0 0.0 0.038631930149837394 1.2974619697678387E-4 11 3.243654924419597E-5 0.0 0.0 0.05001715893455018 1.2974619697678387E-4 12 3.243654924419597E-5 0.0 0.0 0.06493797158688032 1.2974619697678387E-4 13 3.243654924419597E-5 0.0 0.0 0.08427015493642112 1.2974619697678387E-4 14 3.243654924419597E-5 0.0 0.0 0.11132223700608056 1.6218274622097984E-4 15 3.243654924419597E-5 0.0 0.0 0.13996370998870558 1.6218274622097984E-4 16 3.243654924419597E-5 0.0 0.0 0.17259487852836675 1.6218274622097984E-4 17 3.243654924419597E-5 0.0 0.0 0.20402589474599261 1.6218274622097984E-4 18 3.243654924419597E-5 0.0 0.0 0.23506767237268816 1.6218274622097984E-4 19 3.243654924419597E-5 0.0 0.0 0.26841244499572164 1.946192954651758E-4 20 3.243654924419597E-5 0.0 0.0 0.30944467978962953 1.946192954651758E-4 21 3.243654924419597E-5 0.0 0.0 0.35102833592068877 1.946192954651758E-4 22 3.243654924419597E-5 0.0 0.0 0.40020214457488984 1.946192954651758E-4 23 3.243654924419597E-5 0.0 0.0 0.4485974760472302 2.5949239395356774E-4 24 3.243654924419597E-5 0.0 0.0 0.4968306247733496 2.919289431977637E-4 25 3.243654924419597E-5 0.0 0.0 0.5484371746208654 2.919289431977637E-4 26 3.243654924419597E-5 0.0 0.0 0.6012763133396606 2.919289431977637E-4 27 3.243654924419597E-5 0.0 0.0 0.6548939292403165 2.919289431977637E-4 28 3.243654924419597E-5 0.0 0.0 0.7081547430992863 2.919289431977637E-4 29 3.243654924419597E-5 0.0 0.0 0.7620642879431401 4.5411168941874355E-4 30 3.243654924419597E-5 0.0 0.0 0.8236612949578682 4.865482386629395E-4 31 3.243654924419597E-5 0.0 0.0 0.890837388442598 4.865482386629395E-4 32 3.243654924419597E-5 0.0 0.0 0.9629114008632015 4.865482386629395E-4 33 3.243654924419597E-5 0.0 0.0 1.0392994743332828 4.865482386629395E-4 34 3.243654924419597E-5 0.0 0.0 1.1198718626558657 4.865482386629395E-4 35 3.243654924419597E-5 0.0 0.0 1.2030716114672284 4.865482386629395E-4 36 3.243654924419597E-5 0.0 0.0 1.292726233578186 4.865482386629395E-4 37 3.243654924419597E-5 0.0 0.0 1.3823808556891437 4.865482386629395E-4 38 3.243654924419597E-5 0.0 0.0 1.470316340690159 4.865482386629395E-4 39 3.243654924419597E-5 0.0 0.0 1.5623063943466986 4.865482386629395E-4 40 3.243654924419597E-5 0.0 0.0 1.6571184277874835 4.865482386629395E-4 41 3.243654924419597E-5 0.0 0.0 1.7625047762818762 4.865482386629395E-4 42 3.243654924419597E-5 0.0 0.0 1.876000262087318 4.865482386629395E-4 43 3.243654924419597E-5 0.0 0.0 1.9944261033778774 4.865482386629395E-4 44 3.243654924419597E-5 0.0 0.0 2.1253400161274523 4.865482386629395E-4 45 3.243654924419597E-5 0.0 0.0 2.258394741127144 4.865482386629395E-4 46 3.243654924419597E-5 0.0 0.0 2.4026076390668396 5.189847879071355E-4 47 3.243654924419597E-5 0.0 0.0 2.5432849531389174 5.189847879071355E-4 48 3.243654924419597E-5 0.0 0.0 2.688211455161985 6.162944356397234E-4 49 3.243654924419597E-5 0.0 0.0 2.836543794855693 6.162944356397234E-4 50 3.243654924419597E-5 0.0 0.0 2.9892226321481234 6.487309848839194E-4 51 3.243654924419597E-5 0.0 0.0 3.1506269011872425 6.487309848839194E-4 52 3.243654924419597E-5 0.0 0.0 3.326951982878692 6.487309848839194E-4 53 3.243654924419597E-5 0.0 0.0 3.5108347805440387 6.487309848839194E-4 54 3.243654924419597E-5 0.0 0.0 3.7029564617174113 6.487309848839194E-4 55 3.243654924419597E-5 0.0 0.0 3.901370833444158 6.487309848839194E-4 56 3.243654924419597E-5 0.0 0.0 4.116587337679398 6.487309848839194E-4 57 3.243654924419597E-5 0.0 0.0 4.3273600346681835 7.460406326165073E-4 58 3.243654924419597E-5 0.0 0.0 4.547928569528716 7.460406326165073E-4 59 3.243654924419597E-5 0.0 0.0 4.7698270029082614 7.460406326165073E-4 60 3.243654924419597E-5 0.0 0.0 4.99918584261397 7.460406326165073E-4 61 3.243654924419597E-5 0.0 0.0 5.236167271392066 7.460406326165073E-4 62 3.243654924419597E-5 0.0 0.0 5.47908458868185 7.460406326165073E-4 63 3.243654924419597E-5 3.243654924419597E-5 0.0 5.734133175388963 7.460406326165073E-4 64 3.243654924419597E-5 3.243654924419597E-5 0.0 5.989116888997588 7.784771818607032E-4 65 3.243654924419597E-5 3.243654924419597E-5 0.0 6.260351313777554 7.784771818607032E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 500 0.0 27.689545 59 CTCGCGC 540 0.0 27.605282 36 ATTCGCG 510 0.0 26.450115 56 CGCGCTT 590 0.0 24.667486 38 TCGCGCT 595 0.0 24.458607 37 CGATCGT 610 0.0 24.435877 1 TCGCGGA 585 0.0 22.452621 58 CAATTCG 605 0.0 22.297516 54 CCTCGCG 715 0.0 21.839773 35 AATTCGC 635 0.0 21.24409 55 CGCTCCG 710 0.0 20.99863 25 GACGTAT 800 0.0 20.854893 39 ATCGTTC 800 0.0 19.519611 3 ACGTATT 895 0.0 19.434488 40 ACGCTCC 795 0.0 18.755016 24 AACTCGA 805 0.0 18.51903 13 GCGCCCT 805 0.0 18.516928 31 CGGCCAA 775 0.0 18.32674 50 GATCGTT 835 0.0 18.276392 2 CGTATTA 940 0.0 18.128538 41 >>END_MODULE