FastQCFastQC Report
Thu 26 May 2016
SRR936164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082942
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78810.2556324445935084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56490.18323406668046302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41290.13393051182928514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13100.049.528492100-101
GGTATCA80550.042.1117671
CGTACAT19200.039.83839100-101
CGGTATC12550.034.149837100-101
CCGTACA9250.034.10625100-101
GTATCAA166650.032.7102851
GCGTATC4050.031.599726100-101
ACGTATC5500.030.303669100-101
TCGGTAT3700.029.762531100-101
CCGTATC7350.029.155132100-101
TACGCAG13350.028.53636100-101
AGTATCA36200.028.529278100-101
AGGTATC21550.027.345621100-101
ACCGTAC3950.027.12314698-99
CGAGTAC14350.026.96257100-101
CCTGTCG10850.026.60495696-97
ACGTACA8750.026.531172100-101
GCGTACA6800.026.261057100-101
GGGTATC14150.025.871315100-101
TCGTATC5750.025.362852100-101
Produced by FastQC (version 0.11.5)