FastQCFastQC Report
Thu 26 May 2016
SRR936163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3291759
Sequences flagged as poor quality0
Sequence length77
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34720.10547552235749943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT5000.023.4238931
ATTCGCG4950.022.94909156
CGCGGAA4700.021.9048759
TCGCGCT5700.020.55258437
CTCGCGC5700.020.55039836
CGCGCTT5450.020.19138538
TCGCGGA5600.019.65144258
CAATTCG5650.019.47931154
CGGTTAC4250.019.2073424
CCTCGCG6100.019.2013735
AATTCGC6350.017.88890655
CGTTCGG1854.1609565E-617.26734211
ACTCGAG6800.017.22502114
CGCTCCG6850.017.09981225
AACTCGA6850.017.09903113
GATCGTT7150.016.8762052
ATCGTTC7350.016.4164923
GACGCTC7650.015.3146223
ACGCTCC8150.015.24582624
GCGTTCG1901.0070965E-414.94506210
Produced by FastQC (version 0.11.5)