##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936163_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3291759 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.197324895291544 39.0 36.0 41.0 29.0 41.0 2 36.055367054514015 39.0 36.0 41.0 28.0 41.0 3 35.958729360199214 39.0 35.0 41.0 27.0 41.0 4 35.83455471679427 39.0 35.0 41.0 27.0 41.0 5 35.75889182652801 39.0 35.0 40.0 27.0 41.0 6 35.6627420172619 39.0 35.0 40.0 27.0 41.0 7 35.49962831422349 39.0 35.0 40.0 27.0 41.0 8 35.31800171276208 38.0 34.0 40.0 26.0 41.0 9 35.23248816210421 38.0 34.0 40.0 26.0 41.0 10-11 35.44635451744797 39.0 35.0 40.0 27.0 41.0 12-13 35.633530127813124 39.0 35.0 41.0 27.0 41.0 14-15 35.582807550613516 39.0 35.0 41.0 27.0 41.0 16-17 35.41859230885372 39.0 35.0 41.0 26.0 41.0 18-19 35.265565917796536 39.0 35.0 41.0 25.5 41.0 20-21 35.080600372019944 39.0 34.5 41.0 24.5 41.0 22-23 34.8419665595203 39.0 34.0 41.0 24.0 41.0 24-25 34.62211054940535 39.0 34.0 40.5 23.5 41.0 26-27 33.697365147327005 37.5 33.0 39.5 22.0 40.5 28-29 33.727743586331805 38.0 33.0 39.5 22.0 40.5 30-31 34.09242520488286 38.0 33.0 40.0 22.0 41.0 32-33 33.929340665583354 38.0 33.0 40.0 21.0 41.0 34-35 33.64988901070826 38.0 33.0 40.0 19.5 41.0 36-37 33.35023007455892 37.0 33.0 40.0 18.0 41.0 38-39 33.060358155016814 37.0 32.0 40.0 17.5 41.0 40-41 32.67784731506772 36.0 32.0 39.5 13.5 41.0 42-43 32.312703785422926 36.0 31.5 39.0 11.0 41.0 44-45 31.942271442107398 35.5 31.0 39.0 8.0 40.5 46-47 31.545156556114833 35.0 31.0 38.5 7.0 40.0 48-49 31.080495716727743 35.0 31.0 37.5 2.0 40.0 50-51 30.639850912536428 35.0 30.0 37.0 2.0 39.0 52-53 30.18278677144955 35.0 30.0 36.5 2.0 39.0 54-55 29.720866867835706 34.0 29.0 36.0 2.0 38.5 56-57 29.268893014342787 34.0 29.0 35.5 2.0 37.0 58-59 28.8369125747055 34.0 29.0 35.0 2.0 37.0 60-61 28.47674632316643 34.0 29.0 35.0 2.0 36.5 62-63 28.12262000346927 34.0 27.5 35.0 2.0 36.0 64-65 27.741069288486795 34.0 27.0 35.0 2.0 36.0 66-67 27.348156411207505 33.5 26.0 35.0 2.0 35.0 68-69 26.976324208424735 33.0 25.5 35.0 2.0 35.0 70-71 26.538235788221435 33.0 25.0 35.0 2.0 35.0 72-73 26.036814055950025 33.0 24.5 35.0 2.0 35.0 74-75 25.434471964685144 33.0 21.5 35.0 2.0 35.0 76-77 23.727456201988055 30.5 9.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7168241893218408 1101 2 0.5241335129398763 1101 3 0.6221663724574213 1101 4 0.7417862806340167 1101 5 0.7423537160331648 1101 6 0.697901459783381 1101 7 0.7910038922685132 1101 8 0.8208705969157322 1101 9 0.7639579245174986 1101 10-11 0.7698357237872742 1101 12-13 0.9887331740598881 1101 14-15 1.009606055769197 1101 16-17 0.902545330858338 1101 18-19 1.0437186397166158 1101 20-21 1.0415177246532608 1101 22-23 0.959809553116493 1101 24-25 1.0433711582093252 1101 26-27 0.9720524257995606 1101 28-29 0.994401980610327 1101 30-31 0.989102741721581 1101 32-33 1.147782755458742 1101 34-35 1.068307513900173 1101 36-37 1.1399627802470533 1101 38-39 1.145681803118599 1101 40-41 1.0517992420284301 1101 42-43 0.907684738613078 1101 44-45 0.7834187413474112 1101 46-47 0.7420937921799613 1101 48-49 0.8169083126597556 1101 50-51 0.8146353463421576 1101 52-53 0.9663110950564864 1101 54-55 0.8851337948384526 1101 56-57 0.8664523560486899 1101 58-59 0.8983303319267222 1101 60-61 0.7660371412389324 1101 62-63 0.7149909071975422 1101 64-65 0.4673822989189311 1101 66-67 0.5005162751005692 1101 68-69 0.5430235671003452 1101 70-71 0.6606289620647878 1101 72-73 0.7914322009281634 1101 74-75 0.6509959370909932 1101 76-77 0.8017824583295514 1102 1 0.7405682760768642 1102 2 0.8203378985991208 1102 3 0.7370366710872958 1102 4 0.7124414761616293 1102 5 0.8816258110856765 1102 6 0.8510525084131544 1102 7 0.7433891928208496 1102 8 0.8402236399054814 1102 9 0.93279941781838 1102 10-11 0.8847447684263585 1102 12-13 1.0029975915676346 1102 14-15 0.760385684847563 1102 16-17 0.872491121250313 1102 18-19 1.0123946153892405 1102 20-21 0.843751695680055 1102 22-23 0.8243932234087268 1102 24-25 0.8260437176816353 1102 26-27 0.7203472942924307 1102 28-29 0.7803829476697715 1102 30-31 0.9579844755937259 1102 32-33 0.8354061847866348 1102 34-35 0.8599031718084333 1102 36-37 0.8510253954358191 1102 38-39 0.975185491820568 1102 40-41 1.033816589594359 1102 42-43 0.9930084157106975 1102 44-45 0.990584286000626 1102 46-47 0.7577990289206333 1102 48-49 0.9338758094157384 1102 50-51 0.9515381510823389 1102 52-53 0.8332805879741016 1102 54-55 0.8846900839350695 1102 56-57 1.0409101268909708 1102 58-59 0.9957767498300214 1102 60-61 0.9082458459777598 1102 62-63 0.7216724696659362 1102 64-65 0.781680814962133 1102 66-67 0.8600818509553285 1102 68-69 0.5915879276509486 1102 70-71 0.704388919840337 1102 72-73 0.6163253192684053 1102 74-75 0.5130687943291967 1102 76-77 0.8618474738259074 1103 1 0.4754803475650604 1103 2 0.39853018833876064 1103 3 0.5756985582243814 1103 4 0.40986897359338315 1103 5 0.5834122557167376 1103 6 0.47212261150279744 1103 7 0.6289726064408825 1103 8 0.6484486136401983 1103 9 0.6022028094733969 1103 10-11 0.5825074681273961 1103 12-13 0.6600221164739111 1103 14-15 0.7553350472081064 1103 16-17 0.7242183454623117 1103 18-19 0.8122387721811961 1103 20-21 0.7569592935305849 1103 22-23 0.7468826906885013 1103 24-25 0.6867868895951972 1103 26-27 0.5905901613614688 1103 28-29 0.7865981775296333 1103 30-31 1.000459174512251 1103 32-33 0.9515495139117789 1103 34-35 0.9156829275983327 1103 36-37 0.8442181134275231 1103 38-39 0.8264635328283632 1103 40-41 0.965408922007164 1103 42-43 0.9494725132366213 1103 44-45 0.9670432951824495 1103 46-47 0.5723017725213033 1103 48-49 0.7789239738361502 1103 50-51 0.8040116995485604 1103 52-53 0.6547338879730376 1103 54-55 0.8724542381727112 1103 56-57 0.8508066013881788 1103 58-59 0.8223835186453634 1103 60-61 0.8413446120543178 1103 62-63 0.7806211893949779 1103 64-65 0.7896913585787182 1103 66-67 0.809782860059105 1103 68-69 0.8116641818593457 1103 70-71 0.8310419601512713 1103 72-73 0.8502451129776105 1103 74-75 0.8111920873392613 1103 76-77 0.9368614236935571 1104 1 0.5814618990299749 1104 2 0.5073628680345692 1104 3 0.408778563655666 1104 4 0.394243121857599 1104 5 0.5231838443383907 1104 6 0.35673472545278173 1104 7 0.4908218803036135 1104 8 0.4653625128779382 1104 9 0.49572609908883436 1104 10-11 0.32729070725565634 1104 12-13 0.5252411668114334 1104 14-15 0.4920330527959891 1104 16-17 0.4093741827576167 1104 18-19 0.3767583956780456 1104 20-21 0.3188094098374279 1104 22-23 0.36979831049445977 1104 24-25 0.44491013223198195 1104 26-27 0.23288586122935584 1104 28-29 0.31813804644533406 1104 30-31 0.45468492376718217 1104 32-33 0.4719109918164506 1104 34-35 0.506930825204698 1104 36-37 0.5234281783551893 1104 38-39 0.6527689991013617 1104 40-41 0.6358986991955575 1104 42-43 0.6018402767655289 1104 44-45 0.4236306135242387 1104 46-47 0.3743806768285758 1104 48-49 0.4647658084217525 1104 50-51 0.4796900900640253 1104 52-53 0.4745982247366065 1104 54-55 0.4332707746569824 1104 56-57 0.5452807130804089 1104 58-59 0.49984234047239795 1104 60-61 0.28477904859300196 1104 62-63 0.3005756676792437 1104 64-65 0.3105705431721013 1104 66-67 0.29098480977213015 1104 68-69 0.1978282265425868 1104 70-71 0.19054002203424503 1104 72-73 0.07125477729832141 1104 74-75 0.02095997911790093 1104 76-77 -0.002738653216635356 1105 1 0.22425338427428443 1105 2 0.3980268443405137 1105 3 0.4263768267210253 1105 4 0.4421398025660608 1105 5 0.431684440229688 1105 6 0.169136934380532 1105 7 0.21061550131674522 1105 8 0.49822385310236683 1105 9 0.26125054243195933 1105 10-11 0.3389607368732186 1105 12-13 0.4076399537891433 1105 14-15 0.2551010670029825 1105 16-17 0.4214811009966084 1105 18-19 0.46392696801689226 1105 20-21 0.4928589337143734 1105 22-23 0.4874275481075898 1105 24-25 0.5143205538398306 1105 26-27 0.3208376471332812 1105 28-29 0.5090979034314174 1105 30-31 0.5327419641343951 1105 32-33 0.6809183903084346 1105 34-35 0.6489048773835364 1105 36-37 0.638819873636443 1105 38-39 0.48530967900743605 1105 40-41 0.6725038297606574 1105 42-43 0.6602213994011379 1105 44-45 0.5357953509190736 1105 46-47 0.419418259279869 1105 48-49 0.5179473369714387 1105 50-51 0.7068600783821992 1105 52-53 0.5821666342378293 1105 54-55 0.532387557551953 1105 56-57 0.6701367551821953 1105 58-59 0.6548855868410008 1105 60-61 0.40151415426045745 1105 62-63 0.3416703376970638 1105 64-65 0.36774306845323324 1105 66-67 0.47072037563286884 1105 68-69 0.24079110428457184 1105 70-71 0.05611048470548852 1105 72-73 0.11980575845399244 1105 74-75 -0.07937071105402183 1105 76-77 -0.0670220496790499 1106 1 0.2613603025132818 1106 2 0.4002281022021492 1106 3 0.20499317892228675 1106 4 0.20157376483021494 1106 5 0.04300519494667299 1106 6 0.25561492180191436 1106 7 0.28042682207146186 1106 8 0.3894188216558305 1106 9 0.22603041664579848 1106 10-11 -0.028334860611053614 1106 12-13 0.196319199072164 1106 14-15 0.172396664487259 1106 16-17 0.2876760695500664 1106 18-19 0.2760338862558882 1106 20-21 0.14977717270809165 1106 22-23 0.3995344663465872 1106 24-25 0.4746979123303987 1106 26-27 0.284202427007493 1106 28-29 0.3889721172678975 1106 30-31 0.43116963709036327 1106 32-33 0.4317045538304498 1106 34-35 0.47280424845271796 1106 36-37 0.4058010057119148 1106 38-39 0.40968074818982103 1106 40-41 0.42470508762229287 1106 42-43 0.477674229589816 1106 44-45 0.3920846590951754 1106 46-47 0.5992924731163534 1106 48-49 0.39829324892112083 1106 50-51 0.34050787712433817 1106 52-53 0.41565720027556097 1106 54-55 0.50785925566516 1106 56-57 0.3879040507796816 1106 58-59 0.40913086985986524 1106 60-61 0.41597956306548767 1106 62-63 0.4262615326656167 1106 64-65 0.3636550181387648 1106 66-67 0.319776979406452 1106 68-69 0.18450179610846718 1106 70-71 -0.15143668510583197 1106 72-73 -0.19843323525669732 1106 74-75 -0.33833297520496686 1106 76-77 -0.49453777294948864 1107 1 0.41446438041955247 1107 2 0.3437214216236484 1107 3 0.38910180715750897 1107 4 0.4094035187646554 1107 5 0.447627981371042 1107 6 0.2570756643460399 1107 7 0.18949414955022803 1107 8 0.26139517153844594 1107 9 0.17150762152652987 1107 10-11 0.3971266934567055 1107 12-13 0.6183013455521831 1107 14-15 0.4899045623512137 1107 16-17 0.5021545206992428 1107 18-19 0.4670694483809186 1107 20-21 0.3027790855852288 1107 22-23 0.364004222599668 1107 24-25 0.1032932518660914 1107 26-27 0.29865406889370405 1107 28-29 0.41983827383398875 1107 30-31 0.503419876200006 1107 32-33 0.478146167603164 1107 34-35 0.5419757697697065 1107 36-37 0.5507724574697264 1107 38-39 0.42170868778927684 1107 40-41 0.4816139071452312 1107 42-43 0.47765249234956997 1107 44-45 0.5673585483222041 1107 46-47 0.5324620525732122 1107 48-49 0.48426258492089147 1107 50-51 0.3962486822717146 1107 52-53 0.44196723128735727 1107 54-55 0.4908418948559543 1107 56-57 0.42388787827294294 1107 58-59 0.4457095731610323 1107 60-61 0.4689503192316202 1107 62-63 0.42671801471065507 1107 64-65 0.35061847059217754 1107 66-67 0.5067491267559596 1107 68-69 0.3996809109680477 1107 70-71 0.32931281493764075 1107 72-73 0.2773761146549063 1107 74-75 0.3279482177347788 1107 76-77 0.35655488565990723 1108 1 0.29956217805780483 1108 2 0.179427535611552 1108 3 0.253862762043525 1108 4 0.23928950106436986 1108 5 0.21423127641599393 1108 6 0.13198073496046447 1108 7 0.03441564277375164 1108 8 0.22790999775073573 1108 9 0.022550040469290877 1108 10-11 0.055639700004796566 1108 12-13 0.14467395113157977 1108 14-15 0.32236573268211544 1108 16-17 0.2953497309035171 1108 18-19 0.18363081385545144 1108 20-21 0.15240837789509953 1108 22-23 0.16631647194767396 1108 24-25 0.21812020915753294 1108 26-27 0.05906459221478855 1108 28-29 0.2772083561891634 1108 30-31 0.3346930032333333 1108 32-33 0.37555188892913094 1108 34-35 0.41668923551760173 1108 36-37 0.44432163612973596 1108 38-39 0.35864088667280214 1108 40-41 0.3936647590302442 1108 42-43 0.40195059692811697 1108 44-45 0.2587309746358102 1108 46-47 0.3229059752067478 1108 48-49 0.43907042363196425 1108 50-51 0.42119666026998104 1108 52-53 0.3267618136422996 1108 54-55 0.41162569146331407 1108 56-57 0.29705290663651596 1108 58-59 0.3392837559069086 1108 60-61 0.30364029883256904 1108 62-63 0.26013772156219517 1108 64-65 0.13314014894836745 1108 66-67 -0.026585650016222928 1108 68-69 0.20121617115255575 1108 70-71 -0.017198370083743697 1108 72-73 0.08673751402696439 1108 74-75 -0.08601332812304108 1108 76-77 0.05161352885532722 1109 1 0.23617074158928375 1109 2 0.22204377257186536 1109 3 0.15437306489183555 1109 4 0.3466187304447459 1109 5 0.15765281735306758 1109 6 0.2301637961920875 1109 7 0.06964976922951394 1109 8 0.2573668613901461 1109 9 0.3723890835475743 1109 10-11 0.17638505945007665 1109 12-13 0.23535993262885313 1109 14-15 0.32722385545886823 1109 16-17 0.29582278088845726 1109 18-19 0.23725765631407825 1109 20-21 0.21476424142024797 1109 22-23 0.08390818056512472 1109 24-25 0.09953627123240949 1109 26-27 0.04131022692520503 1109 28-29 0.1956038106788185 1109 30-31 0.26813842566380686 1109 32-33 0.2689216819089708 1109 34-35 0.3955647845133754 1109 36-37 0.41466481573953473 1109 38-39 0.3689207411363995 1109 40-41 0.4343609390300358 1109 42-43 0.42003301404876225 1109 44-45 0.29623001852620234 1109 46-47 0.19583041469044105 1109 48-49 0.364533770874921 1109 50-51 0.35458093890062514 1109 52-53 0.4781667517949906 1109 54-55 0.5788696594740124 1109 56-57 0.5362376780059179 1109 58-59 0.5339986180524292 1109 60-61 0.3282437140088881 1109 62-63 0.36149341416799885 1109 64-65 0.3939436209718927 1109 66-67 0.3788230030354427 1109 68-69 0.3601601162166226 1109 70-71 0.4542442647560385 1109 72-73 0.6514433297230227 1109 74-75 0.5277723560123384 1109 76-77 0.6519178786110835 1110 1 -0.600412600463855 1110 2 -0.5725664705362732 1110 3 -0.5911665257730192 1110 4 -0.6131071556250447 1110 5 -0.4646746544360738 1110 6 -0.668244793641172 1110 7 -0.8484200516198115 1110 8 -0.661001268551459 1110 9 -0.5135725823572557 1110 10-11 -0.5848041015578929 1110 12-13 -0.45397895935020927 1110 14-15 -0.5357943533281215 1110 16-17 -0.6469998105639334 1110 18-19 -0.5437946680185846 1110 20-21 -0.7172605705532362 1110 22-23 -0.6130300666991602 1110 24-25 -0.5330759801843286 1110 26-27 -0.5161252878277836 1110 28-29 -0.548136746691064 1110 30-31 -0.5306714587391284 1110 32-33 -0.3447447295886832 1110 34-35 -0.24039431134388423 1110 36-37 -0.29629184775128437 1110 38-39 -0.3199271799704846 1110 40-41 -0.28934921834865435 1110 42-43 -0.2911536686990601 1110 44-45 -0.335438291135155 1110 46-47 -0.27295488904328735 1110 48-49 -0.2371097745424109 1110 50-51 -0.2643109300196045 1110 52-53 -0.2164454188488243 1110 54-55 -0.15416307008171515 1110 56-57 -0.25430473053251035 1110 58-59 -0.2768772725149482 1110 60-61 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-0.8033650016540435 2314 34-35 -0.8085115427742338 2314 36-37 -0.7897420134225115 2314 38-39 -0.8673003172616163 2314 40-41 -0.6378285575114901 2314 42-43 -0.5884969208738866 2314 44-45 -0.6269854693559438 2314 46-47 -0.6716686117893111 2314 48-49 -0.638485379423404 2314 50-51 -0.6054753602703968 2314 52-53 -0.5998197278237853 2314 54-55 -0.6075074096690258 2314 56-57 -0.4596245309280249 2314 58-59 -0.4127442967002004 2314 60-61 -0.5838766955956309 2314 62-63 -0.5771262991220389 2314 64-65 -0.36806334877307734 2314 66-67 -0.45629566743290084 2314 68-69 -0.22373026870338464 2314 70-71 -0.20656057248457316 2314 72-73 -0.23351578242650817 2314 74-75 -0.09590646150706661 2314 76-77 -0.2233504994711808 2315 1 -1.3773775071778545 2315 2 -1.2806098780896562 2315 3 -1.2455020722681027 2315 4 -1.3149172086121013 2315 5 -1.2114266029806586 2315 6 -1.2255502552917648 2315 7 -1.3389796403836627 2315 8 -1.2451341791335153 2315 9 -1.1284370291821233 2315 10-11 -1.281603009211878 2315 12-13 -1.3236907314144304 2315 14-15 -1.561228854317008 2315 16-17 -1.3466312153530495 2315 18-19 -1.3796354030777493 2315 20-21 -1.424001886696587 2315 22-23 -1.39616232279608 2315 24-25 -1.601673258703137 2315 26-27 -1.4938594730256085 2315 28-29 -1.3791020049025562 2315 30-31 -1.5523062878453402 2315 32-33 -1.5119736729411315 2315 34-35 -1.3633433593185273 2315 36-37 -1.679307083815246 2315 38-39 -1.64968319141245 2315 40-41 -1.5942780560132874 2315 42-43 -1.7533636335731728 2315 44-45 -1.679679020786672 2315 46-47 -1.5422004100349263 2315 48-49 -1.3827293365701827 2315 50-51 -1.2021225559976543 2315 52-53 -1.2720691002362372 2315 54-55 -1.3299924490683708 2315 56-57 -1.3724491180640541 2315 58-59 -1.2640281707023497 2315 60-61 -1.2176402266118949 2315 62-63 -1.3566262019102986 2315 64-65 -1.447016435531694 2315 66-67 -1.241988100928637 2315 68-69 -1.3271429725621182 2315 70-71 -1.3341958326117513 2315 72-73 -2.806445100915038 2315 74-75 -3.3585173257766066 2315 76-77 -3.1046197631208514 2316 1 -3.2021226550237643 2316 2 -3.132959136346045 2316 3 -2.5085378018510625 2316 4 -2.4840426973037566 2316 5 -2.9642775577683764 2316 6 -2.7105871345183488 2316 7 -3.0914544158865667 2316 8 -2.9080648764805233 2316 9 -2.4299982352898866 2316 10-11 -2.5960111724265076 2316 12-13 -2.7170460147469555 2316 14-15 -2.7052828006611804 2316 16-17 -3.9941830082880756 2316 18-19 -3.836653505619509 2316 20-21 -3.2099152760719463 2316 22-23 -3.2846676246892734 2316 24-25 -3.357156102817175 2316 26-27 -3.034563809764421 2316 28-29 -3.158827058815376 2316 30-31 -3.2438047601601596 2316 32-33 -3.3644129410923966 2316 34-35 -3.4639128426120678 2316 36-37 -3.367651112807124 2316 38-39 -3.4116062745431037 2316 40-41 -3.439282258544239 2316 42-43 -3.622257207114977 2316 44-45 -3.537238300061926 2316 46-47 -3.209034929636598 2316 48-49 -3.2802235852482795 2316 50-51 -3.6851065888630643 2316 52-53 -4.766657953659159 2316 54-55 -3.974324991556145 2316 56-57 -3.929465606635283 2316 58-59 -4.021280423122377 2316 60-61 -3.595552667469189 2316 62-63 -3.8451261652855813 2316 64-65 -3.813955739066163 2316 66-67 -3.723798032310061 2316 68-69 -3.824458442966243 2316 70-71 -3.557175172547467 2316 72-73 -3.7004018986485256 2316 74-75 -3.6323885085811156 2316 76-77 -3.59734944664606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102792.0 3 8227.0 4 10055.0 5 11507.0 6 13439.0 7 14477.0 8 15795.0 9 15864.0 10 18128.0 11 19708.0 12 16953.0 13 15612.0 14 16175.0 15 16723.0 16 17186.0 17 18012.0 18 19172.0 19 20234.0 20 21701.0 21 23348.0 22 26169.0 23 29609.0 24 33838.0 25 39935.0 26 46431.0 27 54145.0 28 63661.0 29 75674.0 30 88927.0 31 106714.0 32 130120.0 33 162762.0 34 214264.0 35 286607.0 36 400114.0 37 526437.0 38 464767.0 39 125409.0 40 1068.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.135148897333043 26.382401078680417 27.876463384141054 22.60598663984549 2 22.87348446754888 26.48366088532465 27.849915004094083 22.792939643032387 3 22.97127193388896 26.74217052110846 27.428462396898983 22.858095148103597 4 22.662814463808427 26.67502613727134 27.826797926762165 22.835361472158073 5 22.989201751126508 26.71242678194197 27.511912223705227 22.786459243226297 6 23.089613333491318 26.609317924884575 27.61310319704956 22.687965544574553 7 22.964401117506846 26.31673935930215 27.696790434044154 23.022069089146846 8 22.82160828220458 26.70541847793556 27.55471330982216 22.9182599300377 9 22.838457548805103 26.466097654869664 27.74350290759944 22.951941888725795 10-11 23.273804278799552 26.573754751436446 27.39259702653512 22.75984394322888 12-13 23.26874468855042 26.409081807793356 27.37759245074575 22.944581052910472 14-15 22.943896420889786 26.645459352639676 27.492632861047532 22.918011365423006 16-17 23.273425702471442 26.406458133040783 27.561981255275835 22.758134909211936 18-19 23.098909773510847 26.423202703248805 27.430357950759475 23.047529572480872 20-21 23.043080793371022 26.571809400006597 27.436226894741004 22.948882911881377 22-23 23.20766633177006 26.68102473195991 27.033767635436 23.07754130083403 24-25 23.202760048154083 26.74426377547703 27.00615173884006 23.04682443752883 26-27 23.207969085820153 26.7656400936274 26.96670867116977 23.05968214938268 28-29 23.3266914084319 26.68074448904295 27.004019941876955 22.988544160648196 30-31 23.14135559378245 26.614725146120655 27.06279405661564 23.181125203481255 32-33 23.05520341919553 26.89464341731184 27.039596161836048 23.010557001656583 34-35 23.205040797427465 26.642895511432275 27.017312474023086 23.134751217117174 36-37 23.140556883861528 26.732421483647308 27.227276435318622 22.899745197172543 38-39 23.0424278681275 26.687550919989288 27.26645318566391 23.003568026219305 40-41 22.942494435109495 26.677674159083637 27.18862779894662 23.191203606860253 42-43 22.949434476836952 26.84651656842875 26.983956980406653 23.220091974327644 44-45 22.97857846988029 26.616907071281897 27.21623107524812 23.188283383589695 46-47 22.94650029193924 26.565923952372657 27.17689208609921 23.310683669588894 48-49 23.045625370710695 26.602959122157078 27.063384843888254 23.288030663243976 50-51 22.968965153606096 26.70705938592815 27.014274613154672 23.309700847311085 52-53 23.027047676588218 26.65088960243956 27.03276972420478 23.289292996767443 54-55 23.027469520431286 26.61374354224536 27.05615728159411 23.30262965572924 56-57 22.933723519686716 26.847882927078278 26.984585896957135 23.233807656277868 58-59 23.05779397450686 26.76448206801198 27.04046047649193 23.137263480989226 60-61 23.06593766540933 26.693381317610825 26.89080902974648 23.34987198723336 62-63 23.03268692017253 26.58224405546987 27.09386647162398 23.29120255273362 64-65 23.031595298673523 26.67822581110766 26.92612144819914 23.36405744201968 66-67 23.02705109076489 26.688170997414012 27.041594622600513 23.243183289220585 68-69 23.051013892489497 26.47104143372461 27.136185663544094 23.3417590102418 70-71 22.93196053462066 26.405341282459172 27.185344695914477 23.477353487005693 72-73 23.049418323582476 26.292102445102405 27.129408180503027 23.529071050812085 74-75 22.968946777284067 26.272099105667674 27.04804961121141 23.710904505836847 76-77 22.88888061159411 26.15258078027355 27.335845407837095 23.62269320029525 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2973.0 1 1871.5 2 788.5 3 861.0 4 915.0 5 945.0 6 1058.0 7 1196.5 8 1325.0 9 1398.0 10 1507.0 11 1704.5 12 1846.0 13 1899.0 14 2051.0 15 2419.0 16 2853.0 17 3071.0 18 3380.0 19 4218.5 20 5479.5 21 7357.0 22 8503.0 23 10196.0 24 14065.5 25 19272.0 26 22302.0 27 25687.5 28 34064.5 29 42888.0 30 46720.0 31 52640.5 32 64216.0 33 75127.5 34 86728.0 35 93072.0 36 99556.0 37 111274.0 38 119305.5 39 122103.0 40 126492.0 41 134779.0 42 144148.0 43 149619.0 44 150139.0 45 151893.5 46 156061.0 47 159260.0 48 159526.0 49 160449.0 50 159826.0 51 153973.0 52 149666.0 53 148700.5 54 141525.0 55 127780.0 56 113430.5 57 106616.0 58 100489.5 59 84808.5 60 68793.5 61 62333.0 62 55232.5 63 41489.0 64 30340.0 65 25834.0 66 22660.5 67 17394.0 68 13210.0 69 9293.5 70 7468.0 71 6382.5 72 4458.0 73 3194.0 74 2769.0 75 2400.0 76 1687.0 77 1106.5 78 870.0 79 738.0 80 519.5 81 346.5 82 230.5 83 201.0 84 197.0 85 151.0 86 97.0 87 85.0 88 77.0 89 67.0 90 63.5 91 62.0 92 61.5 93 52.0 94 51.5 95 49.0 96 38.0 97 45.0 98 51.5 99 111.5 100 172.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010025035247112563 2 0.01288065134780523 3 0.013609744820322508 4 0.014642627239721984 5 0.011908526717782194 6 0.009660488510853923 7 0.015310962922862823 8 0.016951423236026695 9 0.016890665446650254 10-11 0.019715902652654705 12-13 0.01579702523787434 14-15 0.008642795538798556 16-17 0.006668167384064265 18-19 0.01847036797043769 20-21 0.024743609723555098 22-23 0.02106776346628049 24-25 0.019958933810160465 26-27 0.025579029327481143 28-29 0.008916205590992536 30-31 0.009144047301154186 32-33 0.01037439253602709 34-35 0.011392085508082456 36-37 0.011862958375749865 38-39 0.01347303979422552 40-41 0.011073107113856147 42-43 0.01562994131708913 44-45 0.014202133266742797 46-47 0.02535118761731949 48-49 0.02378667454087617 50-51 0.0374267982558869 52-53 0.04035836159330012 54-55 0.02879919216443245 56-57 0.02071840617736596 58-59 0.016996991578059027 60-61 0.02068802728267774 62-63 0.02598914440577211 64-65 0.026065091642492662 66-67 0.02882957105912067 68-69 0.04313803045727224 70-71 0.055821218989604036 72-73 0.08557734633671542 74-75 0.08582037749422117 76-77 0.09042278003948648 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3291759.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.76252862753911 #Duplication Level Percentage of deduplicated Percentage of total 1 78.52672683660792 46.929557605997246 2 12.112101975940982 14.47699682153692 3 4.035600327641754 7.235330403299896 4 1.75581543976919 4.1972788193552555 5 0.9523623723990917 2.845779177214589 6 0.601186291371856 2.1557047769156767 7 0.37772652907307713 1.5801724754977529 8 0.2744808480486362 1.3122935631374266 9 0.20756707617949513 1.1164259999080501 >10 1.0539991120817318 11.295072879931148 >50 0.07179668376939213 2.9171903467701044 >100 0.028493614429230728 2.9133137483710176 >500 0.0016837013995951561 0.6265107370570953 >1k 4.5919128795488814E-4 0.3983726450077134 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3472 0.10547552235749943 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.075778937643977E-5 0.0 3 0.0 0.0 0.0 1.5189447344109941E-4 0.0 4 3.0378894688219886E-5 0.0 0.0 4.2530452563507837E-4 0.0 5 3.0378894688219886E-5 0.0 0.0 0.0014278080503463346 0.0 6 6.075778937643977E-5 0.0 0.0 0.0030378894688219884 0.0 7 6.075778937643977E-5 0.0 0.0 0.006106157832332197 3.0378894688219886E-5 8 6.075778937643977E-5 0.0 0.0 0.009295941774595284 3.0378894688219886E-5 9 6.075778937643977E-5 0.0 0.0 0.01315406139999921 3.0378894688219886E-5 10 6.075778937643977E-5 3.0378894688219886E-5 0.0 0.016951423236026695 3.0378894688219886E-5 11 6.075778937643977E-5 3.0378894688219886E-5 0.0 0.022541139858659156 3.0378894688219886E-5 12 6.075778937643977E-5 3.0378894688219886E-5 0.0 0.030804199213854962 6.075778937643977E-5 13 6.075778937643977E-5 3.0378894688219886E-5 0.0 0.04189249577505522 6.075778937643977E-5 14 6.075778937643977E-5 3.0378894688219886E-5 0.0 0.056079439594453905 6.075778937643977E-5 15 6.075778937643977E-5 3.0378894688219886E-5 0.0 0.07199798041108113 6.075778937643977E-5 16 6.075778937643977E-5 3.0378894688219886E-5 0.0 0.08740008001800861 6.075778937643977E-5 17 6.075778937643977E-5 3.0378894688219886E-5 0.0 0.10444263993809996 6.075778937643977E-5 18 6.075778937643977E-5 3.0378894688219886E-5 0.0 0.11969284507158634 6.075778937643977E-5 19 1.5189447344109941E-4 3.0378894688219886E-5 0.0 0.13752525625357143 6.075778937643977E-5 20 1.5189447344109941E-4 3.0378894688219886E-5 0.0 0.15818290464156093 6.075778937643977E-5 21 1.5189447344109941E-4 3.0378894688219886E-5 0.0 0.1810582123417905 6.075778937643977E-5 22 2.1265226281753919E-4 3.0378894688219886E-5 0.0 0.20569549593393685 6.075778937643977E-5 23 2.73410052193979E-4 3.0378894688219886E-5 0.0 0.23142641973485908 9.113668406465966E-5 24 3.0378894688219883E-4 3.0378894688219886E-5 0.0 0.25642825006326403 9.113668406465966E-5 25 3.0378894688219883E-4 3.0378894688219886E-5 0.0 0.2861995668577195 9.113668406465966E-5 26 3.3416784157041873E-4 3.0378894688219886E-5 0.0 0.31615315702030433 9.113668406465966E-5 27 3.3416784157041873E-4 3.0378894688219886E-5 0.0 0.34607636828820093 9.113668406465966E-5 28 3.3416784157041873E-4 3.0378894688219886E-5 0.0 0.3753616227676449 1.2151557875287954E-4 29 3.3416784157041873E-4 3.0378894688219886E-5 0.0 0.4078974189787284 1.5189447344109941E-4 30 3.3416784157041873E-4 3.0378894688219886E-5 0.0 0.4410711719782645 1.5189447344109941E-4 31 3.3416784157041873E-4 3.0378894688219886E-5 0.0 0.4761891742378467 1.5189447344109941E-4 32 4.2530452563507837E-4 3.0378894688219886E-5 0.0 0.5163196941209852 1.822733681293193E-4 33 4.2530452563507837E-4 3.0378894688219886E-5 0.0 0.5579995376332229 1.822733681293193E-4 34 4.2530452563507837E-4 3.0378894688219886E-5 0.0 0.6029603017717883 1.822733681293193E-4 35 4.2530452563507837E-4 3.0378894688219886E-5 0.0 0.6496830418022704 1.822733681293193E-4 36 4.2530452563507837E-4 3.0378894688219886E-5 0.0 0.7014790572456854 1.822733681293193E-4 37 4.5568342032329827E-4 3.0378894688219886E-5 0.0 0.7512396867449895 1.822733681293193E-4 38 4.5568342032329827E-4 3.0378894688219886E-5 0.0 0.8015471363486817 2.1265226281753919E-4 39 4.5568342032329827E-4 3.0378894688219886E-5 0.0 0.8554089166308955 2.1265226281753919E-4 40 4.5568342032329827E-4 3.0378894688219886E-5 0.0 0.9105466104900146 2.1265226281753919E-4 41 4.5568342032329827E-4 3.0378894688219886E-5 0.0 0.9708487164461311 2.1265226281753919E-4 42 4.8606231501151817E-4 3.0378894688219886E-5 0.0 1.0399607018618313 2.1265226281753919E-4 43 4.8606231501151817E-4 3.0378894688219886E-5 0.0 1.1080398048581321 2.1265226281753919E-4 44 4.8606231501151817E-4 3.0378894688219886E-5 0.0 1.1810706676886127 2.1265226281753919E-4 45 4.8606231501151817E-4 3.0378894688219886E-5 0.0 1.258202681302003 2.1265226281753919E-4 46 5.16441209699738E-4 3.0378894688219886E-5 0.0 1.3443572266377946 2.1265226281753919E-4 47 5.771989990761778E-4 3.0378894688219886E-5 0.0 1.4292966161860574 2.1265226281753919E-4 48 5.771989990761778E-4 3.0378894688219886E-5 0.0 1.5183979143066062 2.73410052193979E-4 49 5.771989990761778E-4 3.0378894688219886E-5 0.0 1.6090181571615663 2.73410052193979E-4 50 5.771989990761778E-4 3.0378894688219886E-5 0.0 1.6983017286502444 2.73410052193979E-4 51 5.771989990761778E-4 3.0378894688219886E-5 0.0 1.7971850308604003 3.3416784157041873E-4 52 5.771989990761778E-4 3.0378894688219886E-5 0.0 1.8963417431227498 3.3416784157041873E-4 53 5.771989990761778E-4 6.075778937643977E-5 0.0 2.0084398645222814 3.3416784157041873E-4 54 5.771989990761778E-4 6.075778937643977E-5 0.0 2.1246391367047224 3.3416784157041873E-4 55 5.771989990761778E-4 6.075778937643977E-5 0.0 2.244787665196632 3.3416784157041873E-4 56 6.075778937643977E-4 6.075778937643977E-5 0.0 2.376024490249742 3.3416784157041873E-4 57 6.379567884526176E-4 6.075778937643977E-5 0.0 2.5098435213513506 3.645467362586386E-4 58 6.379567884526176E-4 6.075778937643977E-5 0.0 2.648097871077439 4.2530452563507837E-4 59 6.379567884526176E-4 6.075778937643977E-5 0.0 2.7843775926487937 4.2530452563507837E-4 60 6.379567884526176E-4 6.075778937643977E-5 0.0 2.9250318750552515 4.2530452563507837E-4 61 6.683356831408375E-4 6.075778937643977E-5 0.0 3.073766943448776 4.2530452563507837E-4 62 6.683356831408375E-4 6.075778937643977E-5 0.0 3.2245070188917233 4.2530452563507837E-4 63 6.683356831408375E-4 6.075778937643977E-5 0.0 3.381292494377626 4.5568342032329827E-4 64 6.683356831408375E-4 6.075778937643977E-5 0.0 3.547191638269995 4.8606231501151817E-4 65 6.683356831408375E-4 6.075778937643977E-5 0.0 3.724087942039499 4.8606231501151817E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCGT 500 0.0 23.423893 1 ATTCGCG 495 0.0 22.949091 56 CGCGGAA 470 0.0 21.90487 59 TCGCGCT 570 0.0 20.552584 37 CTCGCGC 570 0.0 20.550398 36 CGCGCTT 545 0.0 20.191385 38 TCGCGGA 560 0.0 19.651442 58 CAATTCG 565 0.0 19.479311 54 CGGTTAC 425 0.0 19.207342 4 CCTCGCG 610 0.0 19.20137 35 AATTCGC 635 0.0 17.888906 55 CGTTCGG 185 4.1609565E-6 17.267342 11 ACTCGAG 680 0.0 17.225021 14 CGCTCCG 685 0.0 17.099812 25 AACTCGA 685 0.0 17.099031 13 GATCGTT 715 0.0 16.876205 2 ATCGTTC 735 0.0 16.416492 3 GACGCTC 765 0.0 15.31462 23 ACGCTCC 815 0.0 15.245826 24 GCGTTCG 190 1.0070965E-4 14.945062 10 >>END_MODULE