FastQCFastQC Report
Thu 26 May 2016
SRR936163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3291759
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53520.16258784437135282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38390.11662457670807613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA10400.044.64701100-101
CGGTATC9150.033.830887100-101
GGTATCA64900.033.646611
GTATCAA124100.031.0687081
CGTACAT17550.029.340677100-101
TCGTATC6350.025.780428100-101
TACGCAG9400.025.014992100-101
CCGGTAT4200.024.803896100-101
TCGTATA5050.024.75478100-101
CCTGTCG9150.024.71768896-97
ATCAACG155850.024.5072983
TCAACGC156300.024.4363674
TATCAAC161100.023.7086432
TCGTACA11400.023.498428100-101
TCGAGTA5450.023.484055100-101
CAACGCA162800.023.4234585
ACGTATC3850.023.193253100-101
CGAGTAC12100.023.12297100-101
TATACGT4250.023.10682996-97
AGTATCA32100.022.81031100-101
Produced by FastQC (version 0.11.5)