FastQCFastQC Report
Thu 26 May 2016
SRR936162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311167
Sequences flagged as poor quality0
Sequence length77
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34560.10437407717581143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA4650.024.4334259
TCGCGGA4950.023.66880258
CTCGCGC5300.023.440236
ATTCGCG5150.022.74756256
CGATCGT5750.020.9878061
TCGCGCT6000.020.70738637
CGCGCTT6200.020.0397138
CAATTCG6050.019.95100854
AATTCGC6200.019.4689155
GACGTAT3500.019.2711339
CCTCGCG6450.019.25977535
CGCTCCG6700.018.54308925
CGGTTAC3900.016.3821354
GATCGTT7600.016.3462072
ATCGTTC7650.016.2391243
ACGCTCC8600.015.68580224
AACTCGA7650.015.31163613
CGGCCAA8150.014.81182350
GCGCCCT8400.014.78853331
ACTCGAG8000.014.64131214
Produced by FastQC (version 0.11.5)