##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936162_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3311167 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.17830964128357 39.0 36.0 41.0 29.0 41.0 2 36.04451300704555 39.0 36.0 41.0 28.0 41.0 3 35.90044023753559 39.0 35.0 41.0 27.0 41.0 4 35.78961798060925 39.0 35.0 41.0 27.0 41.0 5 35.71300843479052 39.0 35.0 40.0 27.0 41.0 6 35.62422070526796 39.0 35.0 40.0 27.0 41.0 7 35.48024699448865 39.0 35.0 40.0 27.0 41.0 8 35.35568486880909 38.0 35.0 40.0 26.0 41.0 9 35.53244520738458 39.0 35.0 40.0 27.0 41.0 10-11 35.75894344803508 39.0 35.0 41.0 27.0 41.0 12-13 35.679418011836916 39.0 35.0 41.0 27.0 41.0 14-15 35.52966567376396 39.0 35.0 41.0 26.5 41.0 16-17 35.37713531211202 39.0 35.0 41.0 26.0 41.0 18-19 35.22128225486664 39.0 35.0 41.0 25.0 41.0 20-21 35.03669038740722 39.0 34.5 41.0 24.5 41.0 22-23 34.78384010833642 39.0 34.0 41.0 24.0 41.0 24-25 34.57212562821507 39.0 34.0 40.5 23.0 41.0 26-27 33.6256273090424 37.5 33.0 39.5 21.5 40.5 28-29 33.633224328461836 38.0 33.0 39.5 21.5 40.5 30-31 34.03091764927592 38.0 33.0 40.0 22.0 41.0 32-33 33.90312765861704 38.0 33.0 40.0 20.5 41.0 34-35 33.64259549578743 38.0 33.0 40.0 19.5 41.0 36-37 33.32579752093446 37.0 33.0 40.0 18.0 41.0 38-39 33.00812266490938 37.0 32.0 40.0 16.0 41.0 40-41 32.64444876987479 36.0 32.0 39.5 13.0 41.0 42-43 32.27269630314629 36.0 31.5 39.0 9.5 41.0 44-45 31.906162993289072 35.5 31.0 39.0 8.0 40.5 46-47 31.48929002976896 35.0 31.0 38.5 6.5 40.0 48-49 31.06551285392733 35.0 31.0 37.5 2.0 40.0 50-51 30.610723349199844 35.0 30.0 37.0 2.0 39.0 52-53 30.1214239571728 35.0 30.0 36.5 2.0 39.0 54-55 29.659364206033704 34.0 29.0 36.0 2.0 38.5 56-57 29.21610281209012 34.0 29.0 35.5 2.0 37.0 58-59 28.783624172383938 34.0 29.0 35.0 2.0 37.0 60-61 28.41358107277585 34.0 28.5 35.0 2.0 36.5 62-63 28.033992094026065 34.0 27.5 35.0 2.0 36.0 64-65 27.653061141283423 34.0 27.0 35.0 2.0 36.0 66-67 27.289172065317153 33.5 26.0 35.0 2.0 35.0 68-69 26.9078299282398 33.0 25.5 35.0 2.0 35.0 70-71 26.44337207999476 33.0 25.0 35.0 2.0 35.0 72-73 25.94595712025398 33.0 24.0 35.0 2.0 35.0 74-75 25.35190583863635 33.0 20.5 35.0 2.0 35.0 76-77 23.592479177281 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.003051679613705 1101 2 0.8617294077499835 1101 3 0.9225541235801629 1101 4 1.0214084174247873 1101 5 0.9170635847276003 1101 6 0.8769290158955485 1101 7 0.8895033905655865 1101 8 0.8619926579650397 1101 9 1.043012964857411 1101 10-11 0.9538084995015552 1101 12-13 1.0901498344580816 1101 14-15 1.055463122964504 1101 16-17 1.0430297012838565 1101 18-19 1.1756633198133457 1101 20-21 1.068623201078232 1101 22-23 1.0985004125543014 1101 24-25 1.1144880835832183 1101 26-27 1.1191062955379891 1101 28-29 1.190319627274171 1101 30-31 1.119645052856498 1101 32-33 1.3154538477720337 1101 34-35 1.237952091040924 1101 36-37 1.2948911023816478 1101 38-39 1.2558497433613596 1101 40-41 1.0636690786193341 1101 42-43 1.211847225659362 1101 44-45 1.2242878788355966 1101 46-47 1.14300759360518 1101 48-49 1.2188397975203067 1101 50-51 1.1584520801592397 1101 52-53 1.0624053143406407 1101 54-55 1.1263764478290348 1101 56-57 1.1272829182561814 1101 58-59 1.1567819048497547 1101 60-61 0.988286996231512 1101 62-63 0.993743301926564 1101 64-65 0.9989049278752375 1101 66-67 0.9841732446876783 1101 68-69 1.0379184950124198 1101 70-71 0.9839565167637865 1101 72-73 0.8635057515727134 1101 74-75 0.9115078713706382 1101 76-77 1.0856117232800955 1102 1 0.8828609005993968 1102 2 0.9558470548088067 1102 3 1.0319340917836612 1102 4 0.8102796415901352 1102 5 0.8293052381457215 1102 6 0.8937811621594562 1102 7 0.8678817689439668 1102 8 0.8855220697297455 1102 9 0.9158269553184581 1102 10-11 1.122965892188354 1102 12-13 1.1357778153801803 1102 14-15 1.0438573995940743 1102 16-17 1.0722824834778137 1102 18-19 1.0411641147259019 1102 20-21 1.080546889472501 1102 22-23 1.0891204443508116 1102 24-25 1.2019284651412434 1102 26-27 1.1063876945840931 1102 28-29 1.2159158116938826 1102 30-31 1.3091522070695376 1102 32-33 1.2889037682807754 1102 34-35 1.2414497063032428 1102 36-37 1.1920294171670278 1102 38-39 1.2191247831069845 1102 40-41 1.1277390309245803 1102 42-43 1.1007184498247042 1102 44-45 0.9115692778816964 1102 46-47 0.9943589449565273 1102 48-49 0.8924487005409709 1102 50-51 0.857816150111546 1102 52-53 0.8485738358032755 1102 54-55 0.8673939359053442 1102 56-57 1.0242622505296275 1102 58-59 1.0871475646271769 1102 60-61 0.8889229262792071 1102 62-63 0.7611518869822085 1102 64-65 0.8259319549022628 1102 66-67 1.0293242780740037 1102 68-69 0.9345798622620229 1102 70-71 0.7494573116763483 1102 72-73 0.9057950997444166 1102 74-75 0.7882964246297846 1102 76-77 0.7964225340909046 1103 1 0.7041109173618452 1103 2 0.6465769489347011 1103 3 0.49272113716743604 1103 4 0.6454477056241785 1103 5 0.5013798928765354 1103 6 0.5345526023244673 1103 7 0.5244933504797444 1103 8 0.5711427695408631 1103 9 0.6279903343102191 1103 10-11 0.5619075683040791 1103 12-13 0.6732709676095396 1103 14-15 0.6156471175329727 1103 16-17 0.6865169561113191 1103 18-19 0.5262298409074546 1103 20-21 0.5373242578326511 1103 22-23 0.4317777717261464 1103 24-25 0.5705385328416739 1103 26-27 0.6521026415725402 1103 28-29 0.5980378532458843 1103 30-31 0.7682281417064161 1103 32-33 0.6627840491349772 1103 34-35 0.713916229831014 1103 36-37 0.683556409179161 1103 38-39 0.7000853235581275 1103 40-41 0.6860209469991005 1103 42-43 0.5976548205633776 1103 44-45 0.63699192750083 1103 46-47 0.6750187546642472 1103 48-49 0.5860440545365719 1103 50-51 0.6458584898215634 1103 52-53 0.6685734484044694 1103 54-55 0.7311214375952488 1103 56-57 0.6866436903519393 1103 58-59 0.5768240319870905 1103 60-61 0.41557333212912795 1103 62-63 0.5983970046397005 1103 64-65 0.4396259565283458 1103 66-67 0.5034224459259597 1103 68-69 0.4391552905487899 1103 70-71 0.2921943041404482 1103 72-73 0.0472926643468945 1103 74-75 0.015273040144904826 1103 76-77 0.022993572851149224 1104 1 0.5505811249836654 1104 2 0.6612175206320146 1104 3 0.5859468805603001 1104 4 0.5164991343187921 1104 5 0.5519305202692593 1104 6 0.5810667618625445 1104 7 0.48599638559942093 1104 8 0.41707527994594074 1104 9 0.4149039474306093 1104 10-11 0.5586411329724896 1104 12-13 0.5804529884595979 1104 14-15 0.7027316828260908 1104 16-17 0.6203965614607867 1104 18-19 0.4318901019173538 1104 20-21 0.576297109512808 1104 22-23 0.6254726333625769 1104 24-25 0.6088198653091723 1104 26-27 0.4289154226266376 1104 28-29 0.37884728711781435 1104 30-31 0.6246865825282057 1104 32-33 0.6697651302919141 1104 34-35 0.8469289938520461 1104 36-37 0.6715756276061029 1104 38-39 0.593230747397449 1104 40-41 0.671392999581748 1104 42-43 0.7442967572366896 1104 44-45 0.6999125041740513 1104 46-47 0.654962366861124 1104 48-49 0.5809689968800278 1104 50-51 0.4602279696730669 1104 52-53 0.48991531808169597 1104 54-55 0.5933198471884573 1104 56-57 0.5103427558736051 1104 58-59 0.403573220658604 1104 60-61 0.5485557493233202 1104 62-63 0.4309726869553252 1104 64-65 0.3680035607837766 1104 66-67 0.48128902418382324 1104 68-69 0.2902268054439361 1104 70-71 0.3365264539960897 1104 72-73 0.1486301873874183 1104 74-75 0.29409016841837143 1104 76-77 0.5186031396081958 1105 1 -0.032410481724710394 1105 2 0.28982400646329864 1105 3 0.16066873926259717 1105 4 0.22114481947755849 1105 5 0.06526494060954491 1105 6 0.11461363742655806 1105 7 0.29126455435052634 1105 8 0.3059261983325925 1105 9 0.2617926711104488 1105 10-11 0.4261134164270075 1105 12-13 0.3831224779336253 1105 14-15 0.2880808977035869 1105 16-17 0.27656677386389816 1105 18-19 0.27921530215228074 1105 20-21 0.26237098726734587 1105 22-23 0.26051347712756723 1105 24-25 0.3432466196261146 1105 26-27 0.2025548156826389 1105 28-29 0.40825417260889907 1105 30-31 0.5454710617364569 1105 32-33 0.47573457958583276 1105 34-35 0.390389960181011 1105 36-37 0.42853938034568984 1105 38-39 0.27269859465092594 1105 40-41 0.36560322855714134 1105 42-43 0.36426120623548996 1105 44-45 0.22509844252332556 1105 46-47 0.23868623475776118 1105 48-49 0.10322409037762625 1105 50-51 0.029623525911606663 1105 52-53 0.12065634196076402 1105 54-55 0.0688115986152944 1105 56-57 0.17710375402642242 1105 58-59 0.3806218679460436 1105 60-61 0.2834557401049196 1105 62-63 0.09900043348665122 1105 64-65 0.23555822243770663 1105 66-67 0.20381376298714216 1105 68-69 0.15691978828984432 1105 70-71 0.19636456638610156 1105 72-73 -0.20049335878421104 1105 74-75 -0.5033878838786663 1105 76-77 -0.46789948375138124 1106 1 0.07834027170902402 1106 2 0.011281218646331581 1106 3 0.19227931095241502 1106 4 0.2598124768372543 1106 5 0.3118217828471401 1106 6 0.2538048768050203 1106 7 -0.07715855676863015 1106 8 -0.02973769510985136 1106 9 0.1307063222986642 1106 10-11 -0.08771882395854647 1106 12-13 0.3154778034835317 1106 14-15 0.13732082919195676 1106 16-17 0.23319082715926953 1106 18-19 0.34509384386571895 1106 20-21 0.36435535143222353 1106 22-23 0.38955722840103846 1106 24-25 0.15297291240417366 1106 26-27 0.08072666850289778 1106 28-29 0.3093669022037986 1106 30-31 0.48207872411369834 1106 32-33 0.2625950138207642 1106 34-35 0.25129772413446005 1106 36-37 0.30997895570479983 1106 38-39 0.4534038018397908 1106 40-41 0.2999681191200594 1106 42-43 0.43642172376326727 1106 44-45 0.29569073973425475 1106 46-47 0.3235150791546779 1106 48-49 0.09134373767130555 1106 50-51 0.07190360327463097 1106 52-53 0.2504085254530928 1106 54-55 0.15410468900265428 1106 56-57 0.11651413900000662 1106 58-59 0.006439458288820532 1106 60-61 -0.23169888509890768 1106 62-63 -0.20568526556922961 1106 64-65 -0.08695693529755388 1106 66-67 -0.14616409909504569 1106 68-69 -0.0785066008244435 1106 70-71 -0.4692689503272227 1106 72-73 -0.534821343393034 1106 74-75 -0.365379689550533 1106 76-77 -0.3993760310353913 1107 1 -0.3466624739986415 1107 2 -0.3892674069150601 1107 3 -0.2613644826794612 1107 4 -0.3753596329645177 1107 5 -0.3500557431117741 1107 6 -0.4587692849433296 1107 7 -0.38378536829072374 1107 8 -0.3467550752551105 1107 9 -0.4920620070506274 1107 10-11 -0.37230091988067215 1107 12-13 -0.26337781504516755 1107 14-15 -0.6185432837085969 1107 16-17 -0.44351960129643686 1107 18-19 -0.29076948402043 1107 20-21 -0.42977333048804667 1107 22-23 -0.41118004017634746 1107 24-25 -0.4216971604142046 1107 26-27 -0.7076161255439644 1107 28-29 -0.5708869817747555 1107 30-31 -0.26765619531728646 1107 32-33 -0.17907546735252566 1107 34-35 -0.3855824369667218 1107 36-37 -0.3583375311456578 1107 38-39 -0.22979644759578122 1107 40-41 -0.2745535196362141 1107 42-43 -0.3413852924227676 1107 44-45 -0.3066462997516055 1107 46-47 -0.4748958932091263 1107 48-49 -0.43592408786827264 1107 50-51 -0.3832288614568462 1107 52-53 -0.4223494660304219 1107 54-55 -0.4273390814841953 1107 56-57 -0.48713678610077693 1107 58-59 -0.4958334901147232 1107 60-61 -0.5361607337672929 1107 62-63 -0.5404797207876584 1107 64-65 -0.5116075301763097 1107 66-67 -0.5490954092288085 1107 68-69 -0.6394996403633684 1107 70-71 -0.784327940658649 1107 72-73 -0.6987324436441646 1107 74-75 -0.81957538885095 1107 76-77 -0.4939206047430851 1108 1 0.25300839922632434 1108 2 0.43459309482835096 1108 3 0.3426420095980589 1108 4 0.36784871638843697 1108 5 0.26763322928712796 1108 6 0.3689198343871709 1108 7 0.3695547017396734 1108 8 0.2640391320017059 1108 9 0.3459097876803341 1108 10-11 0.35355577003733885 1108 12-13 0.4210208131734632 1108 14-15 0.34602053235683883 1108 16-17 0.3902314084209877 1108 18-19 0.3275922251858461 1108 20-21 0.4224575634193002 1108 22-23 0.29619243631722725 1108 24-25 0.37546433979937177 1108 26-27 0.21939761570879313 1108 28-29 0.3919359572559742 1108 30-31 0.4603825599901725 1108 32-33 0.4842431121827673 1108 34-35 0.40119287978456697 1108 36-37 0.48208356081497783 1108 38-39 0.5060043628369257 1108 40-41 0.47058269469920333 1108 42-43 0.5404363130288132 1108 44-45 0.32941913623467656 1108 46-47 0.4269007566105465 1108 48-49 0.5058185838309193 1108 50-51 0.3857741604363092 1108 52-53 0.5122940919111798 1108 54-55 0.4927631024440764 1108 56-57 0.4931820327231229 1108 58-59 0.4821171936944282 1108 60-61 0.3227444841585019 1108 62-63 0.39588324293458044 1108 64-65 0.3132628639117243 1108 66-67 0.3467071748781585 1108 68-69 0.32937753636700506 1108 70-71 0.31409781847918694 1108 72-73 0.34334819696359986 1108 74-75 0.4500519611362641 1108 76-77 0.42577554946232254 1109 1 -0.10628804346558951 1109 2 -0.036211811534563765 1109 3 -0.20551499527995531 1109 4 0.036536778439725026 1109 5 -0.11130313873892561 1109 6 -0.05151705611373103 1109 7 0.03243941984653276 1109 8 -0.01167723116582664 1109 9 -0.07632867057885306 1109 10-11 -0.1241925451591186 1109 12-13 -0.05926114056806142 1109 14-15 0.013010177887203156 1109 16-17 0.06474270692863371 1109 18-19 -0.016553720333781996 1109 20-21 -0.11579384877339294 1109 22-23 0.07701094336180603 1109 24-25 0.019068559084594483 1109 26-27 -0.03578172692316883 1109 28-29 0.14640553681873314 1109 30-31 0.16817539225704792 1109 32-33 0.11924906764075871 1109 34-35 0.23751158294385277 1109 36-37 0.2656377493348643 1109 38-39 0.29883952795004376 1109 40-41 0.2469464208046972 1109 42-43 0.27359442538840284 1109 44-45 0.3033703090321893 1109 46-47 0.2650749085703694 1109 48-49 0.43080335047023866 1109 50-51 0.31055315155485275 1109 52-53 0.3368523244513959 1109 54-55 0.3883927570942589 1109 56-57 0.430209014697958 1109 58-59 0.43144797973232585 1109 60-61 0.27266537080420505 1109 62-63 0.15110368797530072 1109 64-65 0.3375219798199893 1109 66-67 0.29762962534756454 1109 68-69 0.13703429155666313 1109 70-71 0.3173182975962625 1109 72-73 0.20848724166984667 1109 74-75 0.18969353934931732 1109 76-77 0.3593100123390336 1110 1 -0.2859927561351938 1110 2 -0.24613434341638651 1110 3 -0.40496681641419485 1110 4 -0.3021794612304163 1110 5 -0.3672358204789603 1110 6 -0.3896930481042631 1110 7 -0.23283506614565397 1110 8 -0.5225177205921199 1110 9 -0.411115883034725 1110 10-11 -0.508235771153629 1110 12-13 -0.4853581935658937 1110 14-15 -0.410931549525138 1110 16-17 -0.5357588118420082 1110 18-19 -0.634097504086391 1110 20-21 -0.6681662004524895 1110 22-23 -0.6921704243248925 1110 24-25 -0.7853574032927426 1110 26-27 -0.717331985407732 1110 28-29 -0.5930349128486156 1110 30-31 -0.5104107141588727 1110 32-33 -0.4682746615411659 1110 34-35 -0.5805547320725637 1110 36-37 -0.6235473573395822 1110 38-39 -0.516702970332382 1110 40-41 -0.5301035590583325 1110 42-43 -0.6224501272772542 1110 44-45 -0.6236862114843547 1110 46-47 -0.6601002727911371 1110 48-49 -0.7609876658649171 1110 50-51 -0.664569189566496 1110 52-53 -0.6193786007884192 1110 54-55 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-0.5156888588122328 2314 30-31 -0.6261065343364649 2314 32-33 -0.7685409300209542 2314 34-35 -0.7621635134611253 2314 36-37 -1.2736324659860898 2314 38-39 -0.8577983262119133 2314 40-41 -0.7343325849062836 2314 42-43 -0.8274471446751335 2314 44-45 -0.743231678119102 2314 46-47 -0.5678501749541915 2314 48-49 -0.5597405202341434 2314 50-51 -0.3781402191911418 2314 52-53 -0.21249608491603666 2314 54-55 -0.456869111715708 2314 56-57 -0.37674870978791475 2314 58-59 -0.38719371081265663 2314 60-61 -0.3601262319557712 2314 62-63 -0.34673069758911623 2314 64-65 -0.2777069547514479 2314 66-67 -0.25210151078458765 2314 68-69 -0.28942228558118543 2314 70-71 -0.26931420623813906 2314 72-73 -0.24748194044341076 2314 74-75 -0.19684618584200564 2314 76-77 -0.25106585807999693 2315 1 -1.2245380640630472 2315 2 -1.1151293048140474 2315 3 -0.9479097422569183 2315 4 -0.9657061093689379 2315 5 -1.074845953035414 2315 6 -1.080977013053861 2315 7 -1.540042245220242 2315 8 -1.439402977735952 2315 9 -1.113103280712032 2315 10-11 -1.2010067024164144 2315 12-13 -1.1435597603146732 2315 14-15 -1.1134063366137283 2315 16-17 -1.011730314868906 2315 18-19 -0.9339163097251699 2315 20-21 -0.8961404545354412 2315 22-23 -1.0205609034543954 2315 24-25 -1.0796728722121784 2315 26-27 -1.0446160415897694 2315 28-29 -1.0617793830300002 2315 30-31 -1.1494269100600292 2315 32-33 -1.08769784903118 2315 34-35 -1.1313524495471228 2315 36-37 -1.2492422393221574 2315 38-39 -1.1163065990567702 2315 40-41 -1.2312980304949264 2315 42-43 -1.241956816941844 2315 44-45 -1.154809102193397 2315 46-47 -1.0709491875843256 2315 48-49 -1.025941902693308 2315 50-51 -1.0388707103316683 2315 52-53 -1.1003288540043634 2315 54-55 -1.1051465859712941 2315 56-57 -1.3057202054023556 2315 58-59 -1.1770743712266842 2315 60-61 -1.2497977565481335 2315 62-63 -1.079462927405281 2315 64-65 -0.8877129270315898 2315 66-67 -0.9338717794311755 2315 68-69 -1.2012496972868547 2315 70-71 -1.1342874900027233 2315 72-73 -1.0980091973645436 2315 74-75 -1.1219631223803432 2315 76-77 -1.1570498000676004 2316 1 -2.0047564739449157 2316 2 -1.6455923687051381 2316 3 -1.9063904060447072 2316 4 -2.9042726331044904 2316 5 -2.3935775881597507 2316 6 -2.338802143356652 2316 7 -2.008433137128108 2316 8 -1.7106188796768507 2316 9 -1.9599084095209491 2316 10-11 -1.9744838340712931 2316 12-13 -2.5301657920329674 2316 14-15 -3.590993434074008 2316 16-17 -2.8198922954459675 2316 18-19 -2.255454819468916 2316 20-21 -2.0772083170633096 2316 22-23 -2.618148102194482 2316 24-25 -2.4836702275434916 2316 26-27 -2.3780603079505767 2316 28-29 -2.8358434627574596 2316 30-31 -3.066054489055105 2316 32-33 -3.11518819263555 2316 34-35 -3.0801275020543706 2316 36-37 -3.1486316803969387 2316 38-39 -3.201585612237068 2316 40-41 -2.987687836176974 2316 42-43 -3.0821113445918584 2316 44-45 -2.9215591980927087 2316 46-47 -2.9325242340147817 2316 48-49 -2.7797778749983486 2316 50-51 -2.83406159742875 2316 52-53 -2.859564305478049 2316 54-55 -2.9137780933564876 2316 56-57 -2.7256835294559245 2316 58-59 -2.950218229050108 2316 60-61 -2.6805758639072508 2316 62-63 -2.7435882624198626 2316 64-65 -2.7106554279589297 2316 66-67 -2.647216088962491 2316 68-69 -2.661102385822364 2316 70-71 -2.58152815358795 2316 72-73 -2.4923447765773723 2316 74-75 -2.725028450381295 2316 76-77 -2.9431653843697347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103511.0 3 8191.0 4 9857.0 5 12515.0 6 13998.0 7 14741.0 8 16054.0 9 16347.0 10 18400.0 11 19878.0 12 17179.0 13 16159.0 14 16318.0 15 17078.0 16 17646.0 17 18728.0 18 19609.0 19 20724.0 20 22357.0 21 23902.0 22 26191.0 23 29660.0 24 34482.0 25 39803.0 26 46908.0 27 55354.0 28 64424.0 29 75506.0 30 89628.0 31 106740.0 32 130578.0 33 162939.0 34 213485.0 35 285218.0 36 400943.0 37 531736.0 38 470461.0 39 122974.0 40 945.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.06437301082498 26.40322725809306 27.94307297452225 22.58932675655971 2 22.891618666021525 26.500826212142766 27.8510963658445 22.756458755991208 3 22.994549356923187 26.733280939180293 27.379287556308107 22.89288214758841 4 22.62272055869533 26.745243266265327 27.81083015915688 22.82120601588246 5 23.00825293993806 26.642621392038357 27.54986182676226 22.799263841261315 6 23.06226445926023 26.621566885641336 27.62309736242699 22.69307129267144 7 22.990222967568574 26.31274663083849 27.69874246967936 22.998287931913577 8 22.75412745769388 26.741201598635644 27.59253458540259 22.91213635826789 9 22.81312762281962 26.460132537628294 27.76421363212245 22.962526207429633 10-11 23.23514743592261 26.58320266280903 27.45325539519992 22.72839450606844 12-13 23.26265685850281 26.38853710496049 27.39212945447186 22.956676582064834 14-15 22.95238182971676 26.62051995392779 27.51938716166758 22.90771105468787 16-17 23.236727195572875 26.40228151868684 27.58535496960467 22.775636316135614 18-19 23.10360523491672 26.446257651923165 27.415080907185008 23.035056205975106 20-21 23.060565239322102 26.620207743724343 27.383600991902284 22.935626025051274 22-23 23.209512216486594 26.663157831146982 27.06657826616204 23.060751686204387 24-25 23.218716804582385 26.7591938232527 26.978713095078245 23.04337627708667 26-27 23.183497034767854 26.78921268057349 26.97939893110283 23.047891353555833 28-29 23.32464784350775 26.66549074779379 27.00203208446319 23.007829324235267 30-31 23.156766722220645 26.590960024694237 27.088816494880234 23.163456758204884 32-33 23.060938324703873 26.91692987973623 27.010954143916322 23.011177651643575 34-35 23.171116994205338 26.64835289302753 27.064097704334745 23.11643240843239 36-37 23.18476339481567 26.69640733438694 27.232132002654915 22.886697268142477 38-39 23.034169097478028 26.718551160914426 27.30120584554629 22.946073896061257 40-41 22.977110799701638 26.6818112553532 27.155797011163774 23.18528093378139 42-43 22.94585655384894 26.848171809161343 27.02398320571489 23.181988431274824 44-45 22.971539195434744 26.62613588805917 27.251330246554183 23.150994669951903 46-47 22.962375177724205 26.589851951620957 27.16722341250411 23.280549458150727 48-49 23.040614369263416 26.608955021011337 27.069384611318558 23.28104599840669 50-51 22.950971707097505 26.758124117805654 27.000218259317748 23.29068591577909 52-53 23.048648557201247 26.666634442343483 27.021258083899447 23.26345891655582 54-55 22.985439067186796 26.651251287446982 27.05432282365764 23.308986821708586 56-57 22.955711210590852 26.84216193798926 26.991074461245518 23.211052390174373 58-59 23.040394684160393 26.811920449985372 26.992582756611988 23.155102109242247 60-61 23.07580541946062 26.67008609436261 26.879918731198998 23.374189754977767 62-63 23.033945714467396 26.615960737443206 27.0624268671744 23.287666680914995 64-65 23.03175050529125 26.661404569204283 26.919192971867716 23.387651953636755 66-67 23.029945397844187 26.679599286148402 27.050616645501634 23.239838670505776 68-69 23.020579950770525 26.479542699984172 27.140346298061875 23.35953105118343 70-71 22.94633310272274 26.423849174326953 27.16674868224857 23.463069040701736 72-73 23.075561983830724 26.316382753418566 27.116112701390634 23.491942561360077 74-75 22.964774281237236 26.287096476340054 27.03494614596813 23.71318309645458 76-77 22.89389002412147 26.155939952929742 27.29706277837839 23.653107244570403 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2996.0 1 1891.5 2 784.0 3 821.5 4 862.0 5 935.0 6 1053.0 7 1154.0 8 1302.5 9 1395.0 10 1474.5 11 1653.5 12 1871.0 13 1989.0 14 2098.0 15 2395.5 16 2851.0 17 3118.0 18 3412.0 19 4166.0 20 5423.5 21 7387.5 22 8554.0 23 10200.5 24 14068.0 25 19453.0 26 22617.0 27 25834.5 28 34346.0 29 43404.5 30 47169.0 31 53087.5 32 64834.0 33 76105.5 34 87677.0 35 93805.0 36 99929.0 37 111927.0 38 120542.5 39 123284.0 40 127148.5 41 135375.0 42 144954.5 43 150172.0 44 150866.0 45 152962.5 46 156972.5 47 160382.5 48 161185.0 49 161828.5 50 160651.5 51 155065.0 52 151299.0 53 149482.5 54 141682.5 55 128251.5 56 113903.5 57 107003.0 58 100662.5 59 85175.0 60 69233.5 61 62439.0 62 55261.5 63 41540.0 64 30573.0 65 26150.0 66 22889.0 67 17647.5 68 13364.5 69 9354.5 70 7647.0 71 6465.5 72 4481.0 73 3244.0 74 2810.0 75 2458.5 76 1744.5 77 1164.5 78 947.0 79 771.5 80 490.0 81 332.5 82 249.5 83 218.0 84 189.0 85 136.5 86 100.0 87 87.0 88 77.0 89 60.0 90 47.5 91 42.0 92 40.0 93 39.5 94 35.5 95 29.5 96 29.0 97 30.5 98 32.0 99 78.0 100 124.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0036543007344540463 2 0.008275028109424865 3 0.011023303868394436 4 0.018029897012141034 5 0.014133989617557798 6 0.013107161312008727 7 0.016640658716398176 8 0.01793929451459259 9 0.016912466209043516 10-11 0.020476164445949116 12-13 0.019917449044400355 14-15 0.008380731023231386 16-17 0.006478078574713991 18-19 0.012019931341427358 20-21 0.010781697208265243 22-23 0.01440579711020314 24-25 0.02269592563588608 26-27 0.01404338712000935 28-29 0.008486433937037909 30-31 0.008244827276908714 32-33 0.00964916598890965 34-35 0.010932701370845989 36-37 0.009800170151490395 38-39 0.01642925288878513 40-41 0.01248804424542767 42-43 0.015613830410849105 44-45 0.018014796595882964 46-47 0.016912466209043516 48-49 0.013726278378589784 50-51 0.026712636360533917 52-53 0.03134846415176281 54-55 0.025262996399758757 56-57 0.015055115009300348 58-59 0.017229574950463084 60-61 0.020279859034594148 62-63 0.025293197232274904 64-65 0.021140582761304396 66-67 0.02038556194840067 68-69 0.02884179505292243 70-71 0.05546382891590789 72-73 0.06568681072262439 74-75 0.07693662083488995 76-77 0.07538127796030826 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3311167.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.81743630828472 #Duplication Level Percentage of deduplicated Percentage of total 1 78.582921728545 47.00628915416164 2 12.129018948952394 14.510536369218768 3 3.9832076329550166 7.147958066608807 4 1.7582149981193618 4.206876546651035 5 0.9640899973561119 2.8834696006151805 6 0.559385883206158 2.0076617664262777 7 0.38646175403893857 1.6182005950468543 8 0.2687551072192491 1.2860993206890932 9 0.20607024649020186 1.1093934460014165 >10 1.0594385762947378 11.347733853847325 >50 0.07104488846773165 2.8856489400317287 >100 0.029263884375172126 2.9680756672230806 >500 0.001670706700133702 0.628636476947613 >1k 4.556472797318716E-4 0.39342019653116433 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3456 0.10437407717581143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.020083251614914E-5 3.020083251614914E-5 3 0.0 0.0 0.0 1.2080333006459656E-4 3.020083251614914E-5 4 0.0 0.0 0.0 6.342174828391319E-4 3.020083251614914E-5 5 3.020083251614914E-5 0.0 0.0 0.001479840793291308 3.020083251614914E-5 6 3.020083251614914E-5 0.0 0.0 0.002869079089034168 3.020083251614914E-5 7 3.020083251614914E-5 0.0 0.0 0.00631197399587517 6.040166503229828E-5 8 3.020083251614914E-5 0.0 0.0 0.010026676395361514 6.040166503229828E-5 9 3.020083251614914E-5 0.0 0.0 0.013952784622460903 6.040166503229828E-5 10 3.020083251614914E-5 0.0 0.0 0.018452708667367125 6.040166503229828E-5 11 1.2080333006459656E-4 0.0 0.0 0.02425126851046776 6.040166503229828E-5 12 1.2080333006459656E-4 0.0 0.0 0.03162027164440815 6.040166503229828E-5 13 1.2080333006459656E-4 0.0 0.0 0.043398596325706314 6.040166503229828E-5 14 1.8120499509689485E-4 0.0 0.0 0.05692856929294113 9.060249754844743E-5 15 2.1140582761304397E-4 0.0 0.0 0.07193838305346725 9.060249754844743E-5 16 2.1140582761304397E-4 0.0 0.0 0.08882064842999463 9.060249754844743E-5 17 2.1140582761304397E-4 0.0 0.0 0.10512909798871516 9.060249754844743E-5 18 2.7180749264534227E-4 0.0 0.0 0.12179995753762948 9.060249754844743E-5 19 3.020083251614914E-4 0.0 0.0 0.14085668285531958 9.060249754844743E-5 20 3.3220915767764056E-4 0.0 0.0 0.16100063814359106 9.060249754844743E-5 21 3.3220915767764056E-4 0.0 0.0 0.1821110200723793 9.060249754844743E-5 22 3.624099901937897E-4 0.0 0.0 0.2054562636073626 9.060249754844743E-5 23 3.624099901937897E-4 0.0 0.0 0.2304021512657018 9.060249754844743E-5 24 3.624099901937897E-4 0.0 0.0 0.2553178380915248 9.060249754844743E-5 25 3.624099901937897E-4 0.0 0.0 0.281683164878123 9.060249754844743E-5 26 3.624099901937897E-4 0.0 0.0 0.31058536159607775 9.060249754844743E-5 27 3.624099901937897E-4 0.0 0.0 0.34090699744229147 9.060249754844743E-5 28 3.624099901937897E-4 0.0 0.0 0.36932598083998786 1.2080333006459656E-4 29 3.624099901937897E-4 0.0 0.0 0.39943621085858855 1.2080333006459656E-4 30 3.624099901937897E-4 0.0 0.0 0.43160009748828737 1.2080333006459656E-4 31 3.624099901937897E-4 0.0 0.0 0.4692303348034092 1.2080333006459656E-4 32 3.926108227099388E-4 0.0 0.0 0.509488044547436 1.2080333006459656E-4 33 3.926108227099388E-4 0.0 0.0 0.5512859967497864 1.2080333006459656E-4 34 3.926108227099388E-4 0.0 0.0 0.5948959989031057 1.2080333006459656E-4 35 3.926108227099388E-4 0.0 0.0 0.6423717076184922 1.2080333006459656E-4 36 3.926108227099388E-4 0.0 0.0 0.6934715162358166 1.2080333006459656E-4 37 3.926108227099388E-4 0.0 0.0 0.7468665881243682 1.2080333006459656E-4 38 3.926108227099388E-4 0.0 0.0 0.7986308150570479 1.510041625807457E-4 39 3.926108227099388E-4 0.0 0.0 0.8516634769554058 1.510041625807457E-4 40 3.926108227099388E-4 0.0 0.0 0.9052397538390543 1.510041625807457E-4 41 3.926108227099388E-4 0.0 0.0 0.9644635864032228 1.510041625807457E-4 42 4.530124877422371E-4 0.0 0.0 1.0286403555000396 1.510041625807457E-4 43 4.530124877422371E-4 0.0 0.0 1.0960788145086007 1.510041625807457E-4 44 4.8321332025838624E-4 0.0 0.0 1.1711278833112313 1.510041625807457E-4 45 4.8321332025838624E-4 0.0 0.0 1.2499520561783806 1.510041625807457E-4 46 4.8321332025838624E-4 0.0 0.0 1.3345143872235983 1.510041625807457E-4 47 5.738158178068336E-4 0.0 0.0 1.4183518982884282 1.510041625807457E-4 48 5.738158178068336E-4 0.0 0.0 1.5040316601367434 1.510041625807457E-4 49 6.040166503229828E-4 0.0 0.0 1.5923388944139634 2.1140582761304397E-4 50 6.040166503229828E-4 0.0 0.0 1.6839984211004762 2.1140582761304397E-4 51 6.040166503229828E-4 0.0 0.0 1.7795236543490558 2.1140582761304397E-4 52 6.040166503229828E-4 0.0 0.0 1.881240058263446 2.1140582761304397E-4 53 6.040166503229828E-4 0.0 0.0 1.9880000012080332 2.1140582761304397E-4 54 6.040166503229828E-4 0.0 0.0 2.1037899930749493 2.1140582761304397E-4 55 6.040166503229828E-4 0.0 0.0 2.226073163932837 2.1140582761304397E-4 56 6.342174828391319E-4 0.0 0.0 2.354003890471245 2.1140582761304397E-4 57 6.342174828391319E-4 0.0 0.0 2.4868271518772684 3.020083251614914E-4 58 6.644183153552811E-4 0.0 0.0 2.6261133914417485 3.020083251614914E-4 59 6.644183153552811E-4 0.0 0.0 2.7621983427595165 3.020083251614914E-4 60 6.644183153552811E-4 0.0 0.0 2.9037496447627076 3.020083251614914E-4 61 6.946191478714302E-4 0.0 0.0 3.0506162932887406 3.020083251614914E-4 62 6.946191478714302E-4 0.0 0.0 3.197664146809871 3.020083251614914E-4 63 6.946191478714302E-4 0.0 0.0 3.353983655913459 3.020083251614914E-4 64 6.946191478714302E-4 0.0 0.0 3.517702368983503 3.020083251614914E-4 65 6.946191478714302E-4 0.0 0.0 3.6938940258827175 3.020083251614914E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 465 0.0 24.43342 59 TCGCGGA 495 0.0 23.668802 58 CTCGCGC 530 0.0 23.4402 36 ATTCGCG 515 0.0 22.747562 56 CGATCGT 575 0.0 20.987806 1 TCGCGCT 600 0.0 20.707386 37 CGCGCTT 620 0.0 20.03971 38 CAATTCG 605 0.0 19.951008 54 AATTCGC 620 0.0 19.46891 55 GACGTAT 350 0.0 19.27113 39 CCTCGCG 645 0.0 19.259775 35 CGCTCCG 670 0.0 18.543089 25 CGGTTAC 390 0.0 16.382135 4 GATCGTT 760 0.0 16.346207 2 ATCGTTC 765 0.0 16.239124 3 ACGCTCC 860 0.0 15.685802 24 AACTCGA 765 0.0 15.311636 13 CGGCCAA 815 0.0 14.811823 50 GCGCCCT 840 0.0 14.788533 31 ACTCGAG 800 0.0 14.641312 14 >>END_MODULE