FastQCFastQC Report
Thu 26 May 2016
SRR936162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311167
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53000.16006441233559046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39210.11841746429582078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA10900.040.955368100-101
CGGTATC9850.036.559074100-101
GGTATCA63500.034.584081
GTATCAA122300.030.900881
CGTACAT16600.027.609509100-101
TCGAGTA5700.026.628174100-101
CCGTACG2150.026.29576396-97
CGAGTAC11950.023.659292100-101
ATCAACG158450.023.6184183
TCAACGC159100.023.5589644
AACGTAT5200.023.4624998-99
CCGGTAT5500.023.267612100-101
TATCAAC162450.023.256962
TACGCAG9250.023.165243100-101
CCTGTCG10150.023.15963496-97
ACGTATC4650.023.040695100-101
CGTCCTA4200.022.674973100-101
CGTATAG3950.022.603214100-101
CCGTATC6850.022.592216100-101
CAACGCA165750.022.5771875
Produced by FastQC (version 0.11.5)