FastQCFastQC Report
Thu 26 May 2016
SRR936161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742393
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56150.20474818889925694No Hit
AACAAAAATGAAGTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTAC34210.12474506753773072No Hit
TCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGT30430.11096148509713961No Hit
ATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAAT29780.10859129234941892No Hit
TACATCAAGTTGGGGGGACAAAAAAAAGGGAGGCCTCAGACCTGGGCCAT28200.10282990074726708No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAT1350.002682495915.77733544
TATACGG1858.020611E-515.34924112
ATTCGCG2101.3849254E-515.21358356
CGCGGAA1655.8590417E-415.06045959
GTACGTA1655.862287E-415.059360540
ATACGTA1759.1606413E-414.1977891
ATCGGTT2051.9219505E-413.8509978
GCCGTAT3101.9934487E-713.7505868
CCCTATA3102.0103835E-713.74104919
CGTAGTA3102.0117295E-713.74029738
TGATACG1600.00829087613.311398555
CGCGCTT2701.0089936E-513.14658238
TAAGGCG17200.013.00135617
CGCGACC14750.012.76488971
CCGGGGT14500.012.73276948
GACGCGA14600.012.6520269
CCGTATC3406.125483E-712.53752269
TTCCCCG15600.012.51567544
GCCGTAA2851.7594111E-512.45488339
CGGGGTG14550.012.44522149
Produced by FastQC (version 0.11.5)