FastQCFastQC Report
Thu 26 May 2016
SRR936161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742393
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62100.22644456866685408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43750.15953220417350833No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36140.13178271677327064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32660.11909306944701216No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31370.11438914845538185No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG31200.11376925189059336No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28390.10352272631967774No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27590.10060556601479073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA10850.042.515087100-101
GGTATCA59750.039.9361951
GTATCAA120250.033.05611
CCCGTAC2900.032.8383498-99
TAAATCG3300.032.4622296-97
TACGCAG7600.031.326908100-101
TTATACG7750.031.100996-97
CGGTATC9600.030.690578100-101
CCGTACA6850.030.412254100-101
CCTGTCG9400.030.39016396-97
CGTACAT16050.028.740728100-101
TCGAGTA3600.028.107197100-101
GGGTATC11300.026.600147100-101
TCGGTAT3250.026.555578100-101
AGTATCA26500.026.166834100-101
GCGTATC3550.025.988096100-101
CGAGTAC11550.025.251387100-101
AGGTATC17050.025.13502100-101
TATCAAC158450.024.97092
ACGTATC4350.024.62943100-101
Produced by FastQC (version 0.11.5)