FastQCFastQC Report
Thu 26 May 2016
SRR936160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2762533
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56380.20408805976254402No Hit
AACAAAAATGAAGTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTAC35110.12709350440338632No Hit
TCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGT30490.11036972228024064No Hit
ATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAAT30290.10964574902815642No Hit
TACATCAAGTTGGGGGGACAAAAAAAAGGGAGGCCTCAGACCTGGGCCAT28880.10454173760096258No Hit
TTTTTTAAATCTTCCGCCTTAATACTTCATTTTTGTTTTTAATTTCTGAA28710.10392636033669099No Hit
CCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAA28460.10302139377158571No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCG952.5318403E-422.4162225
ACGCGTG900.004862471519.72261419
CGCGGAA1851.9571962E-719.19130759
AATTCGC1902.5958434E-718.6828955
CCGCGAC950.00665520918.67984634
CATAACG1000.00894079517.74746126
GACGTAA3055.7661964E-1016.29127731
AGTGTAC13800.015.69006911
CAATTCG2151.7296712E-514.86022354
ATACGTA2151.7338203E-514.8564561
ACGACCG1707.343858E-414.61846722
TCGCGGA2453.6875936E-614.49087158
ATTCGCG2252.6580383E-514.19899756
ACGAGAC3756.9849193E-1014.19694127
CAGTGTA16800.014.15596910
GCCGTAT2658.262548E-613.40377468
CCCGATA7000.013.18478338
ACGTAAT4052.2118911E-913.14483932
GTGTACA17150.013.04013212
CGTAGTA3052.5075715E-612.80237538
Produced by FastQC (version 0.11.5)